Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F28 I0 R1 54 59.4 1320120 91.9% 1213190 218.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,951,494 T→G 16.7% pseudogene (936/984 nt) ilvG → pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive, acetolactate synthase II, large subunit, cryptic, interrupted

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,951,4940TG16.7% 176.1 / 3.3 79pseudogene (936/984 nt)ilvGpseudogene, acetolactate synthase 2 large subunit, valine‑insensitive, acetolactate synthase II, large subunit, cryptic, interrupted
Reads supporting (aligned to +/- strand):  ref base T (31/31);  new base G (13/3);  total (45/34)
Fisher's exact test for biased strand distribution p-value = 2.74e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGGATGCACGGCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCA  >  NC_000913/3951268‑3951719
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                  
tAGAAGCAGTTTACTCGTACTGTCGGGGGATGCTGGGGATGCACGGCC‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGt                                                                                                                                                                                                                                    <  1:290824/227‑1 (MQ=255)
        gATTATCCGTACTATCTGGGCATGCTGGGGATGCACGGCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCCCGTTTTGATGACCGGGTGACAGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAACTGAACAAGCAGCGGCATGCACATGAGGCGATACAAGGGGATATaaac                                                                                                                                                                                                                           >  1:249010/1‑227 (MQ=255)
        gATTATCCGTACTATCTGGGCATGATGGGGATGCACGGCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGGGACCGGCAAACTGAACACCTTCGCGCCCCACGCCAGCGTTATCCAAATGGATATCGACCCGGCAAAAATGACCAAGCTGCGTCAGGCCCATTTGGAATTACAAGGGGACTTaaaa                                                                                                                                                                                                                           >  1:456767/1‑227 (MQ=255)
           tATCCGTACTATCTGGGGATGGTGGGGA‑GCACGGGACCCACAGCGGCAAGCTTCGCGGTGCAGGGGTGTGCCCTGCTGATCGCCGTGGGCGCACGGTTTGAGGACCGGGTGACCGGCAAACTGAACCCCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGct                                                                                                                                                                                                                        <  1:340223/228‑1 (MQ=255)
               cGTACTATCTGGGCATGCTGGGGATGCACGGCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGGGTTATCCATATGGATATGGACCCGGCAAAAATAAACAAGCTGCTTCAGGCACACGTGGCCTTACAAGGGGAATTAAAtccccgtt                                                                                                                                                                                                                    >  1:473057/1‑221 (MQ=255)
                  aCTATCTGGGGATGGTGGGGATGCACGCCA‑CCAAAGCGGCAAACTGCGCGTGGCAGGAGCTTGACCTTCTGATCGCCGTGGGCGGCCGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCTCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTAc                                                                                                                                                                                                                 <  1:41688/228‑1 (MQ=255)
                   ttttCTGGGCCTGCTGGGGATGCACGGAA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGTCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTAc                                                                                                                                                                                                                 <  2:249010/224‑1 (MQ=255)
                   ccaTCTGGGAGTGGTGGGGATGCACGGAC‑CCAAAGCGGCAAACGTCGCGGTGCAGGCGTGTGACCTGCTGATCGCCGGGGGCGCAAGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTAcc                                                                                                                                                                                                                <  2:589943/226‑1 (MQ=255)
                     aTCTGGGCATGCTGGGGATGCACGGCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTAcc                                                                                                                                                                                                                <  2:103358/226‑1 (MQ=255)
                     aTCTGGGCATGCTGGGGATGCACGGCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTAcc                                                                                                                                                                                                                <  2:542721/226‑1 (MQ=255)
                              tGCTGGGGATGCACGGCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATAGGGATATCGACCCGGCACGACTGAACAAGCTGCGTCAGGCCCATGTGGCCTTAACAGGGGATTTAAATGCCCTGTTACCAGAATTAcagc                                                                                                                                                                                                     >  1:481359/1‑228 (MQ=255)
                              tGCTGGGGATGCACGGCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGAGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGCCAGGCACATGTGGCATTACAAGGGAATTTCAATGCTCTGTTACCAGCATTAcagc                                                                                                                                                                                                     >  2:125724/1‑228 (MQ=255)
                               gCTGGGGATGCAAGGCA‑CCAAAGCGGCAATCTTGGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTAcagca                                                                                                                                                                                                    <  2:366197/228‑1 (MQ=255)
                                cTGGGGATGCACGGCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACTTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCACACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAACGAACAAGTTGGGTCAGGCACATGTGGACTTACACGTGGCTTTTAATGCCCTGTTCACACCCTTAcacatg                                                                                                                                                                                                   >  1:80564/1‑224 (MQ=255)
                                cTGGGGATGCACGGCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACCCGCCAGTGTTATCCATATGGAAATCGACCCGGCAGAAATGACCAAGCGGCGTCAGGCACATGTGGCATTACAAGGGGATTTAACTGCCCTGTTCCCACCATTAcagcac                                                                                                                                                                                                   >  1:328162/1‑227 (MQ=255)
                                      aTGCAGGGCA‑CCAAAGCGGAAAACTTCGGGGTGCAGGAGTGTAACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGtt                                                                                                                                                                                              <  2:523848/227‑1 (MQ=255)
                                        gcgcGGCC‑CAAAAGCGGCAAACTGCGGGGGGCAGGAGTGTGACCTGCTGATCGCCGTGGGCACAGGTTTGGAGGACCGGGTGACCGGCAAACTGAACCCCTTCGCGCCACGCGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTaa                                                                                                                                                                                            <  1:266602/224‑1 (MQ=255)
                                         cACGGCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGACCAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAAt                                                                                                                                                                                          >  2:506084/1‑228 (MQ=255)
                                             gCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAAt                                                                                                                                                                                      >  2:100104/1‑228 (MQ=255)
                                                         gCAAACTTCGCGGTGCGGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCTCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGACATGAACAAGCTGCGTCAGGCACATGTGGCCTTACAAGGTGATTTAAATGCTCTGTTACCACCCATACAACAGCCGTTCAATCAAGGAcagtcacca                                                                                                                                                                           >  1:146484/1‑220 (MQ=255)
                                                         gCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGCCCCGGCAGAAATGACCAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCCATGACTGgcagca                                                                                                                                                                           >  2:41996/1‑228 (MQ=255)
                                                            aaCTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACAGGTAGCaaa                                                                                                                                                                         >  2:447020/1‑226 (MQ=255)
                                                                        cAGGGGTGGGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCt                                                                                                                                                           <  2:566142/228‑1 (MQ=255)
                                                                                tgACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCCCACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTTGGTCAGGACCCTGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTAAAGCAGCCGTTAAATCAATTCATGGCAGCAACA‑CTCCGCGCGACGGCGtggg                                                                                                                                                    >  1:78691/1‑225 (MQ=255)
                                                                                tgACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGCACAAGCTGCGTCAGGCACATGTGGCATTACAAGTGGATTTAAATCCTCTGATACCACAATTACAGGAGCCGTTCACTCAATGACTGACAGAAACA‑CACCGCACAACTGCGcgagg                                                                                                                                                   >  1:142521/1‑226 (MQ=255)
                                                                                tgACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGGGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCCGAAAAA‑CTGCCCGCAGCTGCGtgat                                                                                                                                                    >  2:466501/1‑227 (MQ=255)
                                                                                tgACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACCGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCAAATGTGGCATTACAAGGTGATTTAAATACTCTGTTACCAGCATTACCGCAGCCGTTAAATCAATAACTGTCAGCACCA‑CTGAGcacacacgcgcgcgg                                                                                                                                                   >  1:443824/1‑217 (MQ=255)
                                                                                  aCCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGTCAGCAAAA‑CTTCGCGCCGCTCCGtgatgac                                                                                                                                                 >  2:326751/1‑227 (MQ=255)
                                                                                  aCCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTGAAATCAATGACTGGCAGCAACC‑CTGCGCGCatcgcagtgctgac                                                                                                                                                 >  2:247920/1‑215 (MQ=255)
                                                                                   ccTGGTGATCGCCGGGGGCGCCGGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGaa                                                                                                                                                 <  2:512286/227‑1 (MQ=255)
                                                                                   ccTGCTGATCGCCGGGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGaa                                                                                                                                                 <  2:102748/227‑1 (MQ=255)
                                                                                            cGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTGTCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGGGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CGGCGCGAACCTGCGGGTTGAACAATCCTgg                                                                                                                                        >  1:271976/1‑227 (MQ=255)
                                                                                                       cACTTTTTGGTGACCGGGTGCCCGGCAAACTGAACCCCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCAt                                                                                                                            <  1:636375/228‑1 (MQ=255)
                                                                                                              tgatgaCCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCCCGCAGCTGCGGGATGAACATTCCTGGCGTTACAACCAAccccgg                                                                                                                      >  1:102851/1‑224 (MQ=255)
                                                                                                                    ccGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAGGGGGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGG‑ACCAAAACCTGCCGGCACCCCCGTGATGAAAATTCCGTGCGTTACAGCCATCCCGGGGAcccca                                                                                                               >  1:340804/1‑224 (MQ=255)
                                                                                                                          taCCGGCAAACTGAACACCTTCGCGCACCACGCCAGTGTTATCCATATGGATAGCGACCCGGTAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTAcg                                                                                                         <  2:645925/227‑1 (MQ=255)
                                                                                                                              ggCAAACTGAACACCTGCGCACCACACGCCAGGGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCTTCAGGCAAATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTAcgcg                                                                                                       <  2:481359/226‑1 (MQ=255)
                                                                                                                                 aaaCTGAACCCCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCgtt                                                                                                  <  2:473057/228‑1 (MQ=255)
                                                                                                                                     ttaaCTCCTTCGCGCCACAACCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTTCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCgttgtt                                                                                               <  2:271976/225‑1 (MQ=255)
                                                                                                                                            agtCGGGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGAATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTaaaa                                                                                        <  2:50731/225‑1 (MQ=255)
                                                                                                                                               cgcgCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTaaa                                                                                                                                                                                           >  1:364298/1‑126 (MQ=255)
                                                                                                                                                gcgcCACACGCCAATGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAAt                                                                                                                                                                                          <  2:364298/126‑1 (MQ=255)
                                                                                                                                                 cgcCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTCAATCAATGACTGGCAGCAACA‑CTCCGCGCAGCTGGGTGATGAAAATTCCTGGCGTTACGACCATCCCGGTGCCCACATCAACGCCCCGTTGTTTTTAAAAAAACtt                                                                                  >  1:494948/1‑227 (MQ=255)
                                                                                                                                                   ccACACGCCAGTGTGATCCATACGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCTGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAAACAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTGCGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGt                                                                                 <  2:80564/227‑1 (MQ=255)
                                                                                                                                                    cacaCGCCTGTTTTATCCATATGGATATCGACCCGGCAAAAATGAACAAGCTGCGTCAGGCACATGGGGCATTACAAGGTGATTTAAATGCTCTGTTCCCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCg                                                                               <  1:100104/228‑1 (MQ=255)
                                                                                                                                                      cacGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGCTGATTTAAATGATCTGTTACCAGCATTACAGCAGCCGTACAATCAATGACTGGCAGCAACA‑CTGCGCGCAACGACGTGATGAACATTCCTGGCGTTACGCCCATCCCGGCGACGCTATCCACGACCCGTTGTTTGTCAaacaaccgcccgc                                                                             >  1:406353/1‑221 (MQ=255)
                                                                                                                                                         gCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGCCGTTACGACCATCCCGGTGCCGCCATCTACGCGCCGTTGTTGTTAAAACAACTTCCGGATCg                                                                          >  2:527380/1‑228 (MQ=255)
                                                                                                                                                           cgttgtTTTCCATTTGGATAGCGACCCGGGAAAAATGAACAAGCTGCTCCAGGCCCATGTGGCATTACAAGGTGATTTAAATGCCCTGTTACCAGCATTAACGCAGCCGTTAAATCCATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTa                                                                        <  1:125724/225‑1 (MQ=255)
                                                                                                                                                                   tCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGCCGCAATCTCCGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCg                                                                >  2:182614/1‑228 (MQ=255)
                                                                                                                                                                   tCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGGGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCg                                                                >  2:154881/1‑228 (MQ=255)
                                                                                                                                                                   tCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCACATGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCg                                                                >  2:158113/1‑228 (MQ=255)
                                                                                                                                                                   tCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGAGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCg                                                                >  2:41845/1‑228 (MQ=255)
                                                                                                                                                                        atGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACAAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGt                                                                                                                                                       >  1:270885/1‑136 (MQ=255)
                                                                                                                                                                        atGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGGGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTTCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCTGTGTTGTTAAAACAACTGTCGGATCGTAAACCCACGGATTg                                                           >  2:250578/1‑228 (MQ=255)
                                                                                                                                                                        atGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGGGATTTAAATGCTCTGTTACCAGCATTAAAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGACCGTAAACCTCCGAATTg                                                           >  2:594771/1‑228 (MQ=255)
                                                                                                                                                                         tGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGtg                                                                                                                                                      <  2:270885/136‑1 (MQ=255)
                                                                                                                                                                          ggATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAAACACTGTCGGCTCGTAAACCTGCGGATTGcg                                                         >  2:55692/1‑228 (MQ=255)
                                                                                                                                                                           gATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGCGACGCTATCTCCGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGcgt                                                        >  2:558340/1‑228 (MQ=255)
                                                                                                                                                                              ctCGCCCCGGAAGAAATGACCAAGCTGCGTCAGGCACAGGGGGCATTACAAGGTGATTTAAATGCTCGGTTACCAGCATTACAGCGGCCGTTAAAGCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCAGCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGcgtcgt                                                     <  1:655720/227‑1 (MQ=255)
                                                                                                                                                                              atCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGTCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTTGTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGcgtcgt                                                     >  2:578386/1‑228 (MQ=255)
                                                                                                                                                                               tCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTACATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCACCA‑CTGCGAGCAGCTGCGTGATGAACATTTCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGCTGCTGTTAAACCAACTGTCGGTTCGTAACCCTGCGGATTGCGCCGTg                                                    >  2:160690/1‑228 (MQ=255)
                                                                                                                                                                               tCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCCCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTg                                                    >  1:117969/1‑228 (MQ=255)
                                                                                                                                                                                  aCCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACACCTGTCGGATCGTAAACCTGCGGATTGCGTCGTGAcc                                                 >  2:116887/1‑228 (MQ=255)
                                                                                                                                                                                  aCCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGcgtcgt                                                     >  2:180098/1‑224 (MQ=255)
                                                                                                                                                                                  aCCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGcgtcgt                                                     <  1:180098/224‑1 (MQ=255)
                                                                                                                                                                                    ccGTCAGAAATGAACAAGCTGCGTTAGGCACATGTGGCATTTCAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACcac                                               <  2:78691/228‑1 (MQ=255)
                                                                                                                                                                                     cGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGt                                                                         >  2:430137/1‑201 (MQ=255)
                                                                                                                                                                                     cGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCCATCTACGCGCCGTGGTTGTTAAAACAACTGACGGATCGTAAACCTGCGGAATGCGTCCTGACcaca                                              >  2:96692/1‑228 (MQ=255)
                                                                                                                                                                                      ggCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTTATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTa                                                                        <  1:430137/201‑1 (MQ=255)
                                                                                                                                                                                               gAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgc                                    <  2:102851/228‑1 (MQ=255)
                                                                                                                                                                                               gAACAAGCGTGGGCAGGCACATGTGGACTTACAAGGTGATTTAAAGGCTCCTTTACCAGCATTACGGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGGGATGAACATTCCTGGCGTTACGACCAGCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgc                                    <  1:506084/228‑1 (MQ=255)
                                                                                                                                                                                                  cAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCg                                                                               >  2:584873/1‑182 (MQ=255)
                                                                                                                                                                                                  cAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCg                                                                               <  1:584873/182‑1 (MQ=255)
                                                                                                                                                                                                     gggggggcAGGCACAGGGGGCTATACAAGGGGAATGAAAGGCTCTGGTAACAGCATTAAAGCGGCCGTTAAAACAATGACTGGCAGCAAAA‑CGGCGCGCAGCTGGGTGATGAACATTCCTGGCGTTACGACCAGCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCAc                               <  1:41996/220‑1 (MQ=255)
                                                                                                                                                                                                                 ccaTTTGGCATTACAAGGGTATTTAAATGCTCTGTTCCCAGCATTACAGCCGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTg                  <  1:635384/227‑1 (MQ=255)
                                                                                                                                                                                                                   atttgGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCTTCGTGACCCCAGATGTGGGGCAGCACCAAATGTGGGCTgcg                >  2:635384/4‑228 (MQ=255)
                                                                                                                                                                                                                   aTGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGGTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGCCGTGACCACAGATGTGGGGCCGCACCAAAAGTGGGCTgc                 >  1:446563/1‑227 (MQ=255)
                                                                                                                                                                                                                    ttgggattttaaaGGTGATTTAAATCGTCTTTTCCCAGAATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CCGCGCGCAGCCGCGGGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTgcgc               <  1:527380/217‑1 (MQ=255)
                                                                                                                                                                                                                           ttACAAGTGGTTTGAAATGTTCTGGTGCCAGTATTACAGGAGGCGTTAAATCAATGTCTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATTAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGcaca         <  1:55692/227‑1 (MQ=255)
                                                                                                                                                                                                                                 ggTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGCCGTGACCACAGAAGTGGGGCAGCACCAAATTTGGGCTGGGCAGCACACCCCCca  >  1:321911/1‑228 (MQ=255)
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                  
TAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGGATGCACGGCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCA  >  NC_000913/3951268‑3951719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A