Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F27 I0 R1 322 127.1 2757226 91.8% 2531133 223.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,366,268 T→G 13.4% V240G (GTG→GGG)  puuE → 4‑aminobutyrate aminotransferase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,366,2680TG13.4% 278.4 / 2.5 112V240G (GTG→GGG) puuE4‑aminobutyrate aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base T (51/41);  new base G (11/7);  total (64/48)
Fisher's exact test for biased strand distribution p-value = 7.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

TGGTTCGGTGTATCACGTACCTTATCCGTCAGATTTACACGGCATTTCAACACAGGACTCCCTC‑GACGCCATCGAACGCTTGTTTAAATCAGACATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGGCACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCACGCGGTGCTCAACATTATCGACAAAGAAT  >  NC_000913/1366035‑1366501
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                         
tGGTTCGGTGTATCACGTACCTTATCCGTCAGATTTACACGGCATTTCAACACAGGACTCCCTC‑GACGCCATCGAACGCTTGTTTAAATCAGACATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGGGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGAGGATTGGTG‑AGAAAGTGCaaa                                                                                                                                                                                                                                      >  2:167017/1‑239 (MQ=255)
      ggTGTATCACGTCCCTTACCCGTCAGATTTACACGGCATTTCAACACAGGACCCCCTC‑GACGCCATCGAACGCTTGTTTAAATCAGACATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGATTATTTGCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCt                                                                                                                                                                                                                                <  1:667195/239‑1 (MQ=255)
       gtgtATCACGTACCTTATCCGTCAGATTTACACGGCATTTCAACACAGGACTCCCTC‑GACGCCCTCGAACGCTTGTTTAAATCAGACATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGCTTATTTTCGAACCGGTGAAGGGCGCGGGCGGTTCCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTAGCCGCCTGTGCAGCGGCCCCGGTATTGGGATGATTGCTGCGTGAAG‑CCAAAGCGGCtt                                                                                                                                                                                                                               >  2:706672/1‑239 (MQ=255)
       gtgtATCACGTACCTTATCCGTCAGATTTACACGGCATTTCAACACAGGACTCCCTC‑GACGCCATCGAACGCTTGTTTAAATCAGACATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCtt                                                                                                                                                                                                                               >  1:1082945/1‑239 (MQ=255)
            tCACGTACCTTATCCGTCAGATTTACACGGCATTTCAACACAGGACTCCCTC‑GACGCCATCGAACGCTTGTTTAAATCAGACATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTGGCCGCCTGTGCGACGAGCCCGGTATGGGGAGTGTTGCTT‑ATGAAAGTCAAAGGGCGTTTgcgg                                                                                                                                                                                                                          >  2:697683/1‑238 (MQ=255)
               cctcccTTATCCGTCAGATTTACACGGCATTTCAAAACAGGACTCCCTC‑GACGCCATCGAACGCTTGTTTAAATCAGACATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTa                                                                                                                                                                                                                       <  2:1082945/235‑1 (MQ=255)
                  aCCTTATCCGTCAGATTTACACGGCATTTCAACACAGGACTCCCTC‑GACGCCATCGAACGCTTGTTTAAATCAGACATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAACGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGGATTGTGATGATTGCTG‑GAGAAGGGCAAAGCGGCGTTGGGGGTaggg                                                                                                                                                                                                                    >  2:473143/1‑236 (MQ=255)
                  aCCTTATCCGTCAGATTTACACGGCATTTCAACACAGGACTCCCTC‑GACGCCATCGAACGCTTGTTTAAATCAGACATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGCTTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCg                                                                                                                                                                                                                    >  1:955110/1‑239 (MQ=255)
                    cTTATCCGTCAGATTTTCACGGCATTTCAACACGGGACCCCCCC‑GACGCCATCGAACGCTTGTTTAAATCAGACATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGGGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGt                                                                                                                                                                                                                  <  2:445638/239‑1 (MQ=255)
                    cTTATCCGTCAGATTTACACGGCATTTCAACACCGGACTCCCTC‑GACGCCATCGAACGCTTGTTTAAATCAGACATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCCGGGCGAGGGCGGTTTCAACGTTGCGCCAACAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCCAAGCGGCTTTCCGCGTCCCGGt                                                                                                                                                                                                                  >  1:428355/1‑239 (MQ=255)
                    cTTATCCGTCAGATTTACACGGCATTTCAACACAGGACTCCCTC‑GACGCCATCGAACGCTTGTTTAAATCAGACATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGGGCTGGTTGCCGCTATTCGCCGCCTGGGCGACGGGCACGGTATTGGGATGATTGCTG‑GTGAAGTGCAAACGGGCTTGGCGCGTACCgga                                                                                                                                                                                                                  >  2:1246785/1‑238 (MQ=255)
                    cTTATCCGTCAGATTTACACGGCATTTCAACACAGGACTCCCTC‑GACGCCATCGAACGCTTGTTTAAATCAGACATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCCCGGGATTGTGGGGTTTGCGG‑ATGAAGGGCAAGGCGGCTTTGCGCGGACCgga                                                                                                                                                                                                                  >  2:826173/1‑238 (MQ=255)
                    cTTATCCGTCAGATTTACACGGCATTTCAACACAGGACTCCCTC‑GACGCCATCGAACGCTTGTTTAAATCAGACATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGt                                                                                                                                                                                                                  >  1:725355/1‑239 (MQ=255)
                      tATCCGTCAGATTTACACGGCATTTCAACACAGGACTCCCTC‑GACGCCATCGAACGCTTGTTTAAATCAGACATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGGGCAAAGCGGCTTTGCGCGTACCGGTaa                                                                                                                                                                                                                >  1:489714/1‑239 (MQ=255)
                       acccgtacgATTTACCCGGCATTTCAACACAGGACTCCCCC‑GACGCCATCGAACGCTTGTTTAAATCAGACATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGTTAAg                                                                                                                                                                                                               <  1:1365991/231‑1 (MQ=255)
                       aTCCGTCAGATTTACACGGCATTTCAACACAGGACTCCCTC‑GACGCCATCGAACGCTTGTTTAAATCAGACATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAACAGAGCTGGTTGCCGCTCTTCGCCGCCTGTGCGACGCGCCCGGACTTGTGATGGTTGCTG‑GTGAAGTGCAAAGGGGCTTTGCGGGTACCGGGAAg                                                                                                                                                                                                               >  2:163287/1‑239 (MQ=255)
                       aTCCGTCAGATTTACACGGCATTTCAACACAGGACTCCCTC‑GACGCCATCGAACGCTTGTTTAAATCAGACATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGGGCACGGTATTGTGGTGGTTGCTG‑GTGAAGGGCAAAGCGGCTTTGcccggcccggagaa                                                                                                                                                                                                               >  2:799336/1‑235 (MQ=255)
                       aTCCGTCAGATTTACACGGCATTTCAACACAGGACTCCCTC‑GACGCCATCGAACGCTTGTTTAAATCAGACATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGAGGGTTGCCGCTATTCGCCGCCTGTGCGCCGAGCCCGGGATTGTGATGGTGGTTG‑ATGAAGGGCCAAGCGGCTTTTCGGGACCCGGTaaa                                                                                                                                                                                                               >  2:950897/1‑238 (MQ=255)
                            tCAGATTTACACGGCATTTCAACACAGGACTCCCTC‑GACGCCATCGAACGCTTGTTTAAATCAGACATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGAACCGGTAAGCTGtt                                                                                                                                                                                                          >  1:471527/1‑239 (MQ=255)
                            tCAGATTTACACGGCATTTCAACACAGGACTCCCTC‑GACGCCATCGAACGCTTGTTTAAATCAGACATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGAAGAGCACGGTATTGGGATGATTGCTG‑ATGAATGGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGtt                                                                                                                                                                                                          >  1:826539/1‑239 (MQ=255)
                                ttttaccatgCATTTCAACCCAGGGCTGCCTC‑GACGCCACCGAACGCTTGTTTAAATCAGACACA‑‑‑‑‑‑GAAGCGAAGCCGGTGGCGGGGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGGGCGTACCGGTAAGCTGTTTGcc                                                                                                                                                                                                      <  2:263430/230‑1 (MQ=255)
                                    gacaggCATTTCAACACCGGACCCCCCC‑GGCGCCCTCGAACGCTTGTTTAAATCAGACATC‑‑‑‑‑‑GAAGGGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATg                                                                                                                                                                                                   <  2:39887/234‑1 (MQ=255)
                                    acacGGCATTTCAACACAGGACTCCCTC‑GACGCCATCGAACGCTTGTTTAAATCAGACATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGGGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACAAGCACGGTATTGTGATGATTGCTG‑TTGAAGGGAAAAGCGGCGTTGCGCGAACCGGGAAgggtgtggccctgg                                                                                                                                                                                                  >  2:1315016/1‑226 (MQ=255)
                                     cacGGCATTTCAACACAGGACTCCCTC‑GACGCCATCGAACGCTTGTTTAAATCAGACATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTa                                                                                                                                                                                                                       >  1:769118/1‑217 (MQ=255)
                                      caggCATTTCAACACAGGACTCCCCC‑GGCGCCAGCGAACGATTGTTTAAATCAGGCATCGAAGCGGAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGcc                                                                                                                                                                                                      <  1:578120/237‑1 (MQ=255)
                                           ttttcaacgcgggtaccccGC‑GAAGCCATCGAACGGTTGTTGAAATCAGACAGC‑‑‑‑‑‑GAAGCGAAGGAGGTGCGGGGTATTATGTGCGAACCGGGGCAGGGCGAGGGCGGTTGCAACGTTGCGCCAAAAGAGCTGGTTGCCGGTATTCGCCGCCTGTGCGACGAGCACGGTATTGGGATGATTGCTG‑ATGAAGTGCAAAGCGGCATTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTa                                                                                                                                                                                           <  2:428355/223‑1 (MQ=255)
                                           aTTTCAACACAGGACTCCCTC‑GACGCCATCGAACGCTTGTTTAAATCAGACATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTa                                                                                                                                                                                           <  1:687696/239‑1 (MQ=255)
                                                  accaGGGCCCCCTC‑GACGGCATCGAACGTTTGTTTAAATCAGACATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGAt                                                                                                                                                                                    <  1:473143/237‑1 (MQ=255)
                                                    caGGACTCCCTC‑GACGCCATCGAACGCTTGTTTAAATCAGACATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGGTTGTTGCCATGGTTCATTACGCCGaaaa                                                                                                                                                                                  >  2:687696/1‑239 (MQ=255)
                                                    caGGACTCCCTC‑GACGCCATCGAACGCTTGTTTAAATCAGACATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGcttgtct                                                                                                                                                                                  >  1:39887/1‑233 (MQ=255)
                                                       ggcTCCCTCTGAGGCCAACGGAAGCTGGTTTAAATCAGGCATC‑‑‑‑‑‑GGAGCGAAGCAGGTGGCGGGGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGc                                                                                                                                                                                <  2:891688/237‑1 (MQ=255)
                                                       gACTCCCTC‑GACGCCATCGAACGCTTGTTTAAATCAGACATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGGGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGcc                                                                                                                                                                               >  1:588479/1‑239 (MQ=255)
                                                       gACTCCCTC‑GACGCCATCGAACGCTTGTTTAAATCAGACATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTACAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGcc                                                                                                                                                                               >  1:1177552/1‑239 (MQ=255)
                                                            ccTC‑GACGCCATCGAACGCTTGTTTAAATCAGACATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGCTAAGCCGGAtt                                                                                                                                                                          >  1:218717/1‑239 (MQ=255)
                                                            ccTC‑GACGCCATCGAACGCTTGTTTAAATCAGACATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGCTAAGCCGGAtt                                                                                                                                                                          >  1:1156169/1‑239 (MQ=255)
                                                            ccTC‑GACGCCATCGAACGCTTGTTTAAATCAGACATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGAtt                                                                                                                                                                          >  1:1069390/1‑239 (MQ=255)
                                                               c‑GACGCCATCGAACGCTTGTTTAAATCAGACATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTaa                                                                                                                                                                       >  2:369399/1‑239 (MQ=255)
                                                                       aTCGAACGCTTGTTTAAATCAGACATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGACGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGCATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGAAAACTGTTTGCCAGGGCGCATTACGCCGATAAGCCGGATTTAATGACGaa                                                                                                                                                                >  2:533233/1‑238 (MQ=255)
                                                                                             aCATG‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGcc                                                                                                                                                                               <  2:540256/202‑1 (MQ=255)
                                                                                             aCATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGcc                                                                                                                                                                               >  1:540256/1‑202 (MQ=255)
                                                                                             aCATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGCAAAGCCTCGcgggcggg                                                                                                                                          >  1:1159122/1‑239 (MQ=255)
                                                                                             aCATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGcgggcggg                                                                                                                                          >  1:927553/1‑239 (MQ=255)
                                                                                                                   ggggggcgcgATTATGTTCGAACCGGGGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGc                                                                                                                           <  1:697683/231‑1 (MQ=255)
                                                                                                                     ggggcgctattattTTCGAACCGGGGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGGCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGt                                                                                                                         <  1:1315016/230‑1 (MQ=255)
                                                                                                                       gcggcgATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATTAAGTGCAAAGCGGCTTGGCGCGTACCGGTAAGCTGTTTGCCCTGGACAATTACGCCGGTAAGCCGGATTTAATGCCGATTGCGAAAAACCTCCcgggcggggacgccatttagggcggggg                                                                                                                      >  2:394234/1‑219 (MQ=255)
                                                                                                                            gATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGt                                                                                                                  <  1:533233/238‑1 (MQ=255)
                                                                                                                            gATTAATTTCGCACCGGTCCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGACGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGt                                                                                                                  <  1:467715/238‑1 (MQ=255)
                                                                                                                              ttattTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGGAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTAAGCCGATAAACCGGGTTTAATGACGATGGCGACACGCCCCTGGGGCGGGTTGCCGCTTTCGGGGGTGGTCGGGaaa                                                                                                               >  2:757794/1‑238 (MQ=255)
                                                                                                                              ttagtTGCGAACCGGTGCAGGGCGAGGGGTGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGGGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAAc                                                                                                               <  1:757794/239‑1 (MQ=255)
                                                                                                                                 tgttcgtccctgTGCTGGGCGGGGGGGGTTTCAACGTTGCGCCAAAAGGTCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAAcgcg                                                                                                            <  2:385087/228‑1 (MQ=255)
                                                                                                                                  tttCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGa                                                                                                           <  1:826173/239‑1 (MQ=255)
                                                                                                                                        aCCGGTGCAGGGCGAGGGCGGTTTCAACGTTGGGCCAAAAGGGCTGGTTGCCGGTATTCGCCGCCTGTGCGAGGAGCCCGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAAtatt                                                                                                      <  1:1301594/238‑1 (MQ=255)
                                                                                                                                            gttcAGGGCGAGTGCGGTTTCCACGTTACGCCAAAAGAGCTGGTTGCCGCTCTTCGCCTCCTGTGCGACGAGCGCGGCATTGTGCTGATTGCTG‑ATGAAACGCAAAGCGGCGTTGAGCGGCCAGGTAGTCTGTTTGCCTTGGATCATTAGGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCACGGCCGGGATGGCGCCTTCGGGGCGGGTCGGCAAACGGAATATTATGGa                                                                                                 >  2:377635/4‑239 (MQ=255)
                                                                                                                                                 gggggagggggGTTTCAACGTGGCGCCAAAAGGGTGGGTTGCGTCTATACGCCGCTTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCGTTGCGCGTACCGGTAAGCTGGTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCAc                                                                                            <  2:1159122/239‑1 (MQ=255)
                                                                                                                                                    cgagggcgGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGCCGGTAATGCGAATATTATGGACGCAccc                                                                                          >  1:330209/1‑238 (MQ=255)
                                                                                                                                                            gTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGACGGGCAAAGCGGCTTTGCGCGTACCGGTCAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGCCGATGGCGAAAAGCCTCGCGGGCGGGAGGCCCCTTCCGGGCGGGGCGGGGAAAGCGGAAGTTATGGACGCCCCCGCCCcgcgc                                                                                 >  2:503423/1‑239 (MQ=255)
                                                                                                                                                               tCAACCTTGCCCCAATAGGCGTGGTTGCCGTTATTCGCCGCTGGTGCGACGGACACGGTAGTGTGATGATTGGTG‑ATGAAGGGCAAAGCGGCTTTGCGCGTACCGGTAAGTTGTTTGCGATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCTTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCcgggcggg                                                                              <  2:1156169/239‑1 (MQ=255)
                                                                                                                                                                      tgggcCAAAGGAGGTGGTTCCCGTTTTTCGCCGCTTGGGCGACGAGGACGGTTTGGTGATTATTGCTG‑ATGAAGTGCAAAGCGGATTTGCGCGTACCGGTAAGCTTTTTGCCAGGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTggc                                                                        <  2:955110/235‑1 (MQ=255)
                                                                                                                                                                       gcgcCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGGTAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGTCGGGCTGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTggcg                                                                       >  1:1027454/1‑238 (MQ=255)
                                                                                                                                                                             aaaGGGGTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGGTTGCTG‑ATGAATGGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGGCACCTa                                                                <  1:369756/239‑1 (MQ=255)
                                                                                                                                                                             aaaGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCCTGGATCATTACGCCGATAAGcc                                                                                                                                                                               >  2:105353/1‑128 (MQ=255)
                                                                                                                                                                             aaaGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGcc                                                                                                                                                                               <  1:105353/128‑1 (MQ=255)
                                                                                                                                                                                     ggTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGCCGGTAACCCGAACATTATGGACGCACCCGCGCCGGCCGGGCTTggcggacccttgctcctta                                                        >  1:374291/1‑225 (MQ=255)
                                                                                                                                                                                                ttCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGGGGTCGGTAAAGCGAATATTATGGCAGCACCCGCGCCGGGCGGGCTTGGCGCACCCTACCCCGGGAAcccccggggg                                             >  2:582738/1‑232 (MQ=255)
                                                                                                                                                                                                        cTGGGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGGCACCTACGCCGGTAACCCGCTGGCGGTGGCTGc                                     <  1:950897/239‑1 (MQ=255)
                                                                                                                                                                                                          gtgCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTa                                                                                                                                                                                           <  2:607661/87‑1 (MQ=255)
                                                                                                                                                                                                          gtgCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTa                                                                                                                                                                                           >  1:607661/1‑87 (MQ=255)
                                                                                                                                                                                                          gtgCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGGGGTCGGTAACGCGAATAATATGGACGCACACGCGCCGGGCGGGCTTGGCGGCCCCAACGCCGGCAAACCACTGCCGGGGACTGcc                                    >  1:1333270/1‑238 (MQ=255)
                                                                                                                                                                                                                cgaGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGGCACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCAcg                             <  2:927553/239‑1 (MQ=255)
                                                                                                                                                                                                                aggaaatggTTTTGTGGTTTTTGCTT‑GTGAAGGGCAAAGGGGCTTGGCGGGTACCGGGAAGCTGTGTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGGCACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCAcg                             <  2:69997/232‑1 (MQ=255)
                                                                                                                                                                                                                 gaGCACGGTTTTGTGAGTGTTGCTG‑ATGAAGTGAAAAGGGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGGCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGGCACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCAcg                             <  1:1176280/238‑1 (MQ=255)
                                                                                                                                                                                                                 gaGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGGCACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCAcg                             <  1:394234/238‑1 (MQ=255)
                                                                                                                                                                                                                 gaGCACGGTATTGGTATGATTGCTG‑AAGAAGGGCAAAGCGGGTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGGCACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCAcg                             <  1:799336/238‑1 (MQ=255)
                                                                                                                                                                                                                 cgccccggtttttggttttttGCTG‑GTGAAGTGCAAAGGGGCTTTGCGCGTACCGGTAAGCTTGTTGCCATGGATCATTCCGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGGCACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCAcg                             <  2:725355/219‑1 (MQ=255)
                                                                                                                                                                                                                  agacaggTATTGTGATGATTGCTT‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGGCACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCAcgcg                           <  1:950653/234‑1 (MQ=255)
                                                                                                                                                                                                                  aGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGGCACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCAcgcg                           >  1:1003973/1‑239 (MQ=255)
                                                                                                                                                                                                                   gcctggtttggttatgatTGCTG‑GTGAAGGTCAAAGGGGCTTTGCGCGTCCCGGTAAGGTGTTTGCCATGGATCATTACGCCGATAGGCCGGATTTAATGACGATGGCGAAAAGCCTGGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGGCACCTACGCCTGTAACCCGCTGGCGGTGGCTGCCGCGCAcgcg                           <  2:330209/225‑1 (MQ=255)
                                                                                                                                                                                                                      gggTATTGGGATGATTGCTG‑ATGAAGGGCAAAGCGGCTTTGCCGGTTCCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGGCACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCACGCGGTg                        <  1:1246785/237‑1 (MQ=255)
                                                                                                                                                                                                                       gtttttttgatggTTGTTT‑ATGAGGTTCAAAGCGGGTTTGCGGGTTCCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGGCACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCACGCGGTGCt                      <  2:489714/232‑1 (MQ=255)
                                                                                                                                                                                                                         tATTGTGATGATTGCTG‑ATGAAGGGCAAAGCGGGTTTGCGCGTACCGGTAAGCTGGTTGCCATGGATCATTACGCCGATAAGGCGGATTTAAGGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGGCACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCAcgcg                           <  1:262628/232‑1 (MQ=255)
                                                                                                                                                                                                                                   atTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTACCGCGACTATTATGGACGCCCCCGCGCCGGGCCGGCTTGGCGCCACCTCCCCCGTGAACCCCCCGGCGGGGGGCGCCGCGCCCCCGGGGCGCCCCATTATcaa          >  2:429245/1‑237 (MQ=255)
                                                                                                                                                                                                                                   atTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGGCACCTACGCCGGTCACCCGCTGGCGGGGGGTGCCgcgccccccgggcccaaaaacctgc           >  2:950653/1‑217 (MQ=255)
                                                                                                                                                                                                                                   atTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGGCACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCACGCGGGGCCCAACATTATCGa          >  1:222907/1‑239 (MQ=255)
                                                                                                                                                                                                                                   atTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGGCACCTACGCCGGTAACCCGCTGGCGGGGGCTGCCGCGCACGCGGGGCTCAACATTATCg           >  1:131887/1‑238 (MQ=255)
                                                                                                                                                                                                                                   atTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGGCACCTACGCCGGTAACCCGCTGGCGGGGGCTGCCCCGCCCCCGGGGCCCAAccggtctct          >  1:69997/1‑231 (MQ=255)
                                                                                                                                                                                                                                   atTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGGCACCTACCCCGGAAACCCGCTGGCGGGGGCTGCCCCCCACCCGGGGGCCAACAATaggaa          >  2:483772/1‑235 (MQ=255)
                                                                                                                                                                                                                                   atTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGGCACCACCGCCGGTAACCCCCCGGCGGTGGGGGCCCCGCCCCCGGGGCCCAAAAATAACGa          >  2:130185/1‑239 (MQ=255)
                                                                                                                                                                                                                                   atTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAAAATTATGGCCGCACCCGCGCCGGGCTGGCTTGGCGGCACCTACGCCGGTAACCCGCTGGCGGCGGCGTCCGCGCACGGGGGGTCCACCATTTTCgg          >  1:1322974/1‑238 (MQ=255)
                                                                                                                                                                                                                                   atTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGGAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGGCACCTCCGCCGGTAACCCCCCGGCGGGGGGGTCCCGGCCCGCGGGGCCCAACATTATcca          >  2:1290314/1‑237 (MQ=255)
                                                                                                                                                                                                                                     tggtg‑atgaAGTTCAAAGCGGCTTTGCGCGTGCCGGTAAGCTGTTCGCCATGGACGCTTACGTTGATAAGCCGGATTTAATGACGAGGGCGAAGAGCCTGGCGGGGGGGATGCCGCTTGCGGGCGTGGCCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGGCGCCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCTCGCGGTGCTCAACATTATCGACa        <  2:340427/236‑1 (MQ=255)
                                                                                                                                                                                                                                     tggtg‑aggaAGTGCAAAGCGGCTTGGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGGCACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCACGCGGTGCTCAACATTATCGACa        <  1:1307779/236‑1 (MQ=255)
                                                                                                                                                                                                                                     tGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGa                                                                                                           >  1:86678/1‑140 (MQ=255)
                                                                                                                                                                                                                                     tGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGa                                                                                                           <  2:86678/140‑1 (MQ=255)
                                                                                                                                                                                                                                     tGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGGCACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCCGGCCCCCGGGGCTCAACATTATCGAcc        >  1:229317/1‑238 (MQ=255)
                                                                                                                                                                                                                                     tGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGGCACCTACGCCGGTAACCCGCTGGCGGGGGCGGCCccgcccgccgggtctcacatttacgaca        >  1:848414/1‑219 (MQ=255)
                                                                                                                                                                                                                                     tGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGGCACCTACGCCGGTAACCCCCGGGCGGGGGCTGCCCCGCCCGGGGTGCGCCACATTAACGACa        >  2:1049748/1‑239 (MQ=255)
                                                                                                                                                                                                                                       cTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGGCACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCCCGCGGGGCTCCACAATTTCCACaaa      >  2:1307779/1‑239 (MQ=255)
                                                                                                                                                                                                                                          gaggaAGTGAAAAGCGGGTTTGCGCGTACCGGGAAGCGGTTTGCCATGGACCAGTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCTCAGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGGCACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCACGCGGTGCTCAACATTATCGACAAAGa    <  2:1069390/236‑1 (MQ=255)
                                                                                                                                                                                                                                            tgaAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGGCACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCACGCGGTGCTCAACATTATCGACAAAGAAt  <  1:369399/239‑1 (MQ=255)
                                                                                                                                                                                                                                             gaAGTGCAAAGGGGCTTTGGGCGTACCGGTAAGCTTGTGGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCGCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGGCACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCACGCGGTGCTCAACATTATCGACAAAGAAt  <  2:131887/238‑1 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                         
TGGTTCGGTGTATCACGTACCTTATCCGTCAGATTTACACGGCATTTCAACACAGGACTCCCTC‑GACGCCATCGAACGCTTGTTTAAATCAGACATC‑‑‑‑‑‑GAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTG‑ATGAAGTGCAAAGCGGCTTTGCGCGTACCGGTAAGCTGTTTGCCATGGATCATTACGCCGATAAGCCGGATTTAATGACGATGGCGAAAAGCCTCGCGGGCGGGATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCACCCGCGCCGGGCGGGCTTGGCGGCACCTACGCCGGTAACCCGCTGGCGGTGGCTGCCGCGCACGCGGTGCTCAACATTATCGACAAAGAAT  >  NC_000913/1366035‑1366501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A