Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F27 I0 R1 322 127.1 2757226 91.8% 2531133 223.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,431,467 A→C 11.5% T807P (ACT→CCT)  stfR → Rac prophage, putative tail fiber protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,431,4670AC11.5% 252.1 / 2.1 112T807P (ACT→CCT) stfRRac prophage, putative tail fiber protein
Reads supporting (aligned to +/- strand):  ref base A (60/33);  new base C (2/12);  total (62/50)
Fisher's exact test for biased strand distribution p-value = 7.25e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

TGGTGCATTATGGCGTAATGATGGCGCTAAAACTTACCTGCTGCTTACCAATCAAGGTGATGTTTATGGTGGCTGGAATACATTAAGACCGTTT‑GCTATTGATAACGCAACCGGCGAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTCGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAAGAGACGGTTATGG  >  NC_000913/1431235‑1431702
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                            
tGGTGCATTATGGCGTAATGATGGCGCTAAAACTTACCTGCTGCTTACCAATCAAGGTGATGTTTATGGTGGCTGGAATACATTAAGACCGTTT‑GCTATTGATAACGCAACCGGCGAACT‑GGTTCTTGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AAACAGTTAAAAGAAACAAAGAGCCAAACCCCAAGAGGGGTTTTT‑GGGGTTGAGTTTGATGGTTCAAAAGA‑AATAATTTTa                                                                                                                                                                                                                                         >  2:756859/1‑238 (MQ=255)
 ggTGCATTATGGCGTAATGATGGCGCTAAAACTTACCTGCTGCTTACCAATCAAGGTGATGTTTATGGTGGCTGGAATACATTAAGACCGTTT‑GCTATTGATAACGCAACCGGCGAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGCAC‑GGC‑AAAGCCTTAACAGCACAAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTTAGTTTGATGGTTCCCAAGA‑TATTACTTTaa                                                                                                                                                                                                                                        >  2:1015895/1‑238 (MQ=255)
     cATTATGGCGTAATGATGGCGCTAAAACTTACCTGCTGCTTACCAATCAAGGTGATGTTTATGGTGGCTGGAATACATTAAGACCGTTT‑GCTATTGATAACGCAACCGGCGAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAAGCTGCAAACCCCAAGACGGGTTTAT‑GGTGTTGAGTTTGAAGGTTGCAAACA‑AATTACTTTACccgcc                                                                                                                                                                                                                                   >  2:628664/1‑239 (MQ=255)
      aTTATGGCGTAATGATGGCGCTAAAACTTACCTGCTGCTTACCAATCAAGGTGATGTTTATGGTGGCTGGAATACATTAAGACCGTTT‑GCTATTGATAACGCAACCGGCGAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGGTTCT‑GGTGTTGAGTTTGATGGTTCCAAAAA‑AATAAATTTAAacgccg                                                                                                                                                                                                                                  >  2:383752/1‑239 (MQ=255)
      aTTATGGCGTAATGATGGCGCTAAAACTTACCTGCTGCTTACCAATCAAGGTGATGTTTATGGTGGCTGGAATACATTAAGACAGTTT‑GCTATTGATAACGCAACCGGCGAACT‑TGTTATTGGAACCAAACTTT‑CCGC‑AAGCCTGAAC‑GGT‑AATCCACTAACAGCAACAAAGATGCCAACGCCAAGACTGGTGATT‑GGGGTTGAGTTTGACGGGTCCAAAGA‑GCATAATTTAAccccc                                                                                                                                                                                                                                   >  2:204955/1‑238 (MQ=255)
        tATGGCGTAATGATGGCGCTAAAACTTACCTGCTGCTTACCAATCAAGGTGATGTTTATGGTGGCTGGAATACATTAAGACCGTTT‑GCTATTGATAACGCAACCGGCGAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGCCGGGTTTCT‑GGGGTTGCGTTTGATGGTTCCAAAGA‑TTTTACTTTAACCGCcgcg                                                                                                                                                                                                                                >  1:1226151/1‑239 (MQ=255)
        tATGGCGTAATGATGGCGCTAAAACTTACCTGCTGCTTACCAACCAAGGTGATGTTTATGGTGGCTGGAATACATTAAGACCGTTT‑GCTATTGATAACGCAACCGGCGAACT‑GGTTATTGGACCCAAACTGT‑CCGC‑ACGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAAACTGCAACCGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACagccgc                                                                                                                                                                                                                                 >  1:1368619/1‑238 (MQ=255)
        tATGGCGTAATGATGGCCCTAAAACTTACCTGCTGCTTACCAATCAAGGTGATGTTTATGGGGGCTGAAATACATTAAGACCGTTT‑GCTATTGATAACGCAACCGGCGAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTGGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCcgcg                                                                                                                                                                                                                                <  1:383752/239‑1 (MQ=255)
             cGTAATGATGGCGCTAAAACTTACCTGCTGCTTACCAATCAAGGTGATGTTTATGGTGGCTGGAATACATTAAGACCGTTT‑GCTATTGATAACGCAACCGGCGAACT‑GGTTATTGGAACCAAACCGT‑CCGC‑AAGTCTGAAC‑GGG‑AATGCATTAATATCAACAAAGCTCAAAACGCCACAACGGGTTTCT‑GGTGTTGAGTTTTATGGTTTCAAAGA‑TATTACTTTAACCGCCGCGAAtgg                                                                                                                                                                                                                           >  2:717147/1‑238 (MQ=255)
               tAATGATGGCGCTAAAACTTACCTGCTGCTTACCAATCAAGGTGATGTTTATGGTGGCTGGAATACATTAAGACCGTTT‑GCTATTGATAACGCAACCGGCGAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGGGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTgg                                                                                                                                                                                                                         >  1:1108556/1‑239 (MQ=255)
                               ccTTTCCTGCTGCTTACAAATCAAGGTGATGTTTATGGTGGCTTGAATTCATTAAGCCCGTTT‑GCTAGTGATAACGAAACCGGCGAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAg                                                                                                                                                                                                         <  1:1358280/238‑1 (MQ=255)
                                  tACCTGGTTATTACCAATCAAGGGGATTTTTAGGGGGGCTGGAATTCATTAAGCCCTTTT‑GCTATTGAAAACGCAACCGGCAAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGACT‑GGT‑AATGAATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTCCTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGc                                                                                                                                                                                                      <  1:628664/239‑1 (MQ=255)
                                  tACCTGCTGCTTACCAATCAAGGGGATGTTTAGGGGGGCTGGAATGCATTAAGACTGTTT‑GCTTTTGAAAACGCACCCGGCAAACT‑GGTTATGGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAAAAAAGCTGCAAACGCAAAGACGGGTTTTT‑GGTGTTGAGTTTGAGGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGc                                                                                                                                                                                                      <  1:744205/239‑1 (MQ=255)
                                   aCCTGCTGTTTACCAATCAAGTGGATGTTTATGGTGGCTGGAATACATTAAGGCCGTTT‑GTTATTGATAACGCAACCGGCAAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCa                                                                                                                                                                                                     <  1:695640/239‑1 (MQ=255)
                                        ctgctTACCAATCAAGGTGATGTTTATGGTGGCTGGAATACATTAAGACCGTTT‑GCTATTGATAACGCAACCGGCGAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGa                                                                                                                                                                                                >  1:396079/1‑239 (MQ=255)
                                                   tCAAGGTGATGTTTATGGTGGCTGGAATACATTAAGACCGTTT‑GCTATTGATAACGCAACCGGCGAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCg                                                                                                                                                                                    >  1:1053870/1‑239 (MQ=255)
                                                   tCAAGGTGATGTTTATGGTGGCTGGAATACATTAAGACCGTTT‑GCTATTGATAACGCAACCGGCGAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAAGGCAACGGAAAAA‑AATGCCg                                                                                                                                                                                    >  2:66746/1‑239 (MQ=255)
                                                       ggTGATGTTTATGGTGGCTGGAATACATTAAGACCGTTT‑GCTATTGATAACGCAACCGGCGAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAAAAGGGAAACGGAAACA‑AAAGCCggggc                                                                                                                                                                                >  2:525360/1‑235 (MQ=255)
                                                           aTGTTTATGGTGGCTGGAATACATTAAGACCGTTT‑GCTATTGATAACGCAACCGGCGAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGGTTCT‑GGGGTTGAGTTTGATGGTTCCAAAGA‑TTTTCATTTAACCGCCCCGCATGTGGCTGCTTTTTCCCGAAGGGCAACGGATACA‑AAAACCCATGCggcg                                                                                                                                                                            >  2:661027/1‑237 (MQ=255)
                                                            tGTTTATGGTGGCTGGAATACATTAAGACCGTTT‑GCTATTGATAACGCAACCGGCGAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGAAACA‑TTTGCCGATGCGGAtg                                                                                                                                                                           >  1:1296733/1‑239 (MQ=255)
                                                             gTTTATGGTGGCTGGAATACATTAAGACCGTTT‑GCTATTGATAACGCAACCGGCGAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGAtgg                                                                                                                                                                          >  1:459101/1‑239 (MQ=255)
                                                               tgaggggtgttGGAATTAATTAAGACCGTTG‑GCTATTGGTAACGCACCCGGGTAATT‑GGTTATGGAAACCAAACTGT‑CGTA‑AAGCTGGAAC‑GGT‑AATGCATTAACAGAAACAAAGTTGCAAACGGCAAGAGGGGTGTGT‑GGTGTGGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGAtggtg                                                                                                                                                                        <  1:557064/229‑1 (MQ=255)
                                                                   gggtgggtgaattaaataaatccGTTTGGCTATGGATACCG‑AATCGGGGAAATGGGTTATTGGAACAAACCTGC‑CCGG‑AAGGCCG‑AC‑GGTGAATGCGTTAAAAGAAAAAAAGCGGCAGATGCCAAGACGGTTTGTT‑GGTGTAGATTTTGATGGTTCCAAGTA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCg                                                                                                                                                                     <  2:755750/218‑1 (MQ=255)
                                                                       gCTGGAATACATTAAGACCGTTT‑GCTATTGATAACGCAACCGGCGAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTCCTTTAACCGCGGCGCCTGTGGCTGCTTTTGCCAGCAGGGCAACGGATACA‑CAGGCCGATGCGGATGGTGGCGTTCCa                                                                                                                                                                >  1:648049/1‑239 (MQ=255)
                                                                       gCTGGAATACATTAAGACCGTTT‑GCTATTGATAACGCAACCGGCGAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCa                                                                                                                                                                >  1:424330/1‑239 (MQ=255)
                                                                       gCTGGAATACATTAAGACCGTTT‑GCTATTGATAACGCAACCGGCGAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAAAAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGATGCTTTTGCCAGAAGGGCAAAGGAAACA‑TATGCCGATGCGGATGGGGGGGTCCCa                                                                                                                                                                >  2:1236931/1‑239 (MQ=255)
                                                                                 ataaaGACCGTTT‑GCTTTTGATAACGAACCCGGCGAATG‑GGTTATTGGAACCAAACGGT‑CCGC‑AAGTGTGAAC‑GGT‑AATGCATTAACAACAACAAAGCTCAAAACGCCAAGACGGTTTTCT‑GGGGTTGAGTTTGATGTTTCCAAAGA‑TATTACTTAAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATg                                                                                                                                                              <  1:684363/228‑1 (MQ=255)
                                                                                 aTTAAGACCGTTT‑GCTATTGATAACGCAACCGGCGAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGGTACA‑TATGCCGATGCGGGTGGGGGCGTTCCCTGGAAGGCCg                                                                                                                                                      >  2:909265/1‑239 (MQ=255)
                                                                                     aGACCGTTT‑GCTATTGATAACGCAACCGGCGAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTTCCAGAAGGGCAACGGATACT‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATc                                                                                                                                                  >  1:398801/1‑239 (MQ=255)
                                                                                     aGACCGTTT‑GCTATTGATAACGCAACCGGCGAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGCTTCT‑GGGGTTGAGTTTGATGGTTCCCAAGC‑TATTACTTTAACCGCCCCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCCAGGCGGATGGTGGGGTTCCCTGACATGCCGAAtt                                                                                                                                                  >  2:839199/1‑238 (MQ=255)
                                                                                      gACCGTTT‑GCTATTGATAACGCAACCGGCGAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACTGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCCCGCCTGGGGCGTCTTTTTCCAGAAGGGCAACGTA‑ACATTATCCCGAGTCGGGTGGGGGGGGTCCAAGGAATGCCGaacta                                                                                                                                                 >  2:480119/1‑236 (MQ=255)
                                                                                           ttt‑GCTATTGATAACGCAACCGGCGAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGCTGGTTCCAAAGA‑TATTACTTTAACCGCCCCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATTGGGATGGTGGGGTTCCATGGAAGGGCGAATCTgttct                                                                                                                                            >  2:684363/1‑235 (MQ=255)
                                                                                           ttt‑GCTATTGATAACGCAACCGGCGAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGgc                                                                                                                                              >  1:78392/1‑237 (MQ=255)
                                                                                           ttt‑GCTATTGATAACGCAACCGGCGAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGgc                                                                                                                                              >  2:1338894/1‑237 (MQ=255)
                                                                                           ttt‑GCTATTGATAACGCAACCGGCGAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGgc                                                                                                                                              >  1:107099/1‑237 (MQ=255)
                                                                                           ttt‑GCTATTGATAACGCAACCGGCGAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGgc                                                                                                                                              >  1:1364431/1‑237 (MQ=255)
                                                                                           ttt‑GCTATTGATAACGCAACCGGCGAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGAACCA‑AATGCCGATGCGGAGGGGGGCGTTCCATGGAATGCGGAAACTggg                                                                                                                                              >  2:90865/1‑236 (MQ=255)
                                                                                           ttt‑GCTATTGATAACGCAACCGGCGAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAACCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCACAGA‑TCTTACTTTAACGGCCGCGCCTGTGGCTGCTTTTTCCCTAAAGGCAACGGATACA‑TATGCCTATCCGGATGGTGCGTTTCCATGGAATGCCGAACCTGgc                                                                                                                                              >  2:511473/1‑237 (MQ=255)
                                                                                            tt‑GCTATTGATAACGCAACCGGCGAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCGGAATCTgcggct                                                                                                                                           >  1:186214/1‑234 (MQ=255)
                                                                                              tgttATGGAAAACGCAACCGGCGAACT‑TGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGAAG‑GGT‑AATGCATTAAACGCAACAAAGCTGCAAACGCCAAGGCGGTTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCtt                                                                                                                                          <  1:564617/236‑1 (MQ=255)
                                                                                                  aTTGATAACGCAACCGGCGAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTTCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACaa                                                                                                                                      >  1:148097/1‑239 (MQ=255)
                                                                                                  aTTGATAACGCAACCGGCGAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACaa                                                                                                                                      >  1:946225/1‑239 (MQ=255)
                                                                                                      aTAACGCAACCGGCGAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGCATGCCGAATCTGGGGCTTACAATGt                                                                                                                                   >  1:687536/1‑238 (MQ=255)
                                                                                                        aaCGCAACCGGCGAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGTATTAACAGCAACAAAGCTGCAACCGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCCACGGAAACA‑TATTCCGATGCCGGTGGGGGCGTTCCCTGGATTGCCGAATCAGGCGCCTACAATGTcc                                                                                                                                 >  2:941680/1‑237 (MQ=255)
                                                                                                             aaCCGGCGAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGGGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGCCACCCGc                                                                                                                            >  1:1357283/1‑238 (MQ=255)
                                                                                                                    gAACT‑GTTTATGGGAACCAACCGGC‑CCGC‑AATTCTAAAG‑GGT‑AATGCATTAAAAGAAAAAAAGCTGCAAACGCCAAGCCGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGa                                                                                                                    <  1:1236931/239‑1 (MQ=255)
                                                                                                                     gaatttgtttgtGGAACAAAACTTA‑CCGA‑AAGTTTGAAC‑GTT‑AATGTATTAACAGACACAAAGCCGCAAACGCCAAGACGGGTTTTG‑GGGGTTGAGTTGGGTGTTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCg                                                                                                                     <  2:396079/227‑1 (MQ=255)
                                                                                                                      gaacggTTTTTGGGACCAAAACGTGCCCA‑AAGTC‑GAACGGGT‑AATGCTTTAAAAGCAACAAAGCGGCAAACGCCAAGAGGGGTTCCT‑GGGGTTGAGTTGGATGGTTCAAAAGA‑TTTTCTTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGa                                                                                                                    <  2:1368619/235‑1 (MQ=255)
                                                                                                                      ctattgttttttgcaCCAAACTTT‑TCGCAAACTCGGAAC‑GGT‑ATTTAATTAAAAGCAACTAAGCTGAAAGCGCCAAGGCGGGTTTGT‑GGTGTTGAGTTTGATTGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGa                                                                                                                    <  2:662361/225‑1 (MQ=255)
                                                                                                                              aTTGGACCCAACTTTC‑CCGC‑AAGGTGGAGC‑GTT‑AATGCATTAACAGCAAAAAAGCGGCAACCGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGTTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCCCCCGCTCTGGCGACAGCTatat                                                                                                           <  1:756859/239‑1 (MQ=255)
                                                                                                                               ttGGAACGAAATTTC‑GCGA‑AAGTCTGAGC‑GTA‑AAGGCATTACAAGTACAAAAGTGACAAACGCCAAGAGGGGTTTCT‑GGTGTTAAGTTTGTTGGTGCCAAAGA‑TATTTCTTGAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATAtt                                                                                                          <  2:407268/239‑1 (MQ=255)
                                                                                                                               ttGGAACCAAACTGG‑CCGC‑AAGTCGGAAC‑GTT‑AATGCATTAACAGCAAAAAAGCTGAAAACCCCAAGGCGGGTTTCG‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATAtt                                                                                                          <  1:90865/239‑1 (MQ=255)
                                                                                                                               ttGGAACCAAACTGC‑CCGA‑AAGTCTGAAC‑GGA‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGGCGGGTTTCG‑GGTGTGGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATAtt                                                                                                          <  1:66746/239‑1 (MQ=255)
                                                                                                                                gggAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGAAACAAAGCTGCAAACGCCAAGACGGGTTTCG‑GGTGTTGAGTTTGATGGTTCAAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTc                                                                                                         <  1:207034/238‑1 (MQ=255)
                                                                                                                                     ccAAACTGC‑CCGC‑TGGTCTGAAC‑GTT‑TATGCATTAATAGTAACAACGCTGAAACCGCCAAGACGTGTTTCT‑GGTGTTGATTTGGGTGGTGCAAAAGT‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGtt                                                                                                    <  2:687536/239‑1 (MQ=255)
                                                                                                                                       aaccTGT‑ACGA‑AAGTCTGAAC‑GTT‑AAGTCATTACACGCAAAAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTGGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTaa                                                                                                  <  1:1114222/236‑1 (MQ=255)
                                                                                                                                          aatttccccaaattCTGAAG‑GGT‑ATTGCTTTAAAAGAAAAAAACCTAC‑AACCCCAAAGCGGGTTTTTGGGTTTTGAGTTTGATGGTCCAAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAAc                                                                                                 <  2:107099/226‑1 (MQ=255)
                                                                                                                                             t‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTCCAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCta                                                                                            >  1:263905/1‑239 (MQ=255)
                                                                                                                                             t‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGAGCAACGGATACA‑TATGCCGATGCGGCTGGTGGCGTTCCATGGAATGCCGAATCTGGGGCTTACCAAGTCACCCGCCCTGGCGCCAGCCAAATTCTGGTTAACTTcca                                                                                            >  2:86958/1‑237 (MQ=255)
                                                                                                                                             t‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGCGTCTGCTTTTGCCAGACGGGCAACGGATACA‑TATGCCGACGCCGATGGTGCCTGTCCCGGGAATGCCGACGCTGGCGCTTCCCATGTCCCCCCCCCTGCCGACAGcccaatccctgttaactccac                                                                                            >  2:1339593/1‑219 (MQ=255)
                                                                                                                                                 gC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCCCTGGCGACAGCTATATTCTGGTTAACTTCtata                                                                                          >  1:1260799/1‑238 (MQ=255)
                                                                                                                                                            aC‑GGT‑AATGATTTAACAGCAACAAAGCGTCAAACGCAAAGCAGGGTTTTG‑GGTGTTGAGTTTGATGTTTCAAAAGA‑TTTTCCTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTCGGa                                                                               <  1:1187857/239‑1 (MQ=255)
                                                                                                                                                               ggT‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAAc                                                                                                                                                                                                                                       >  2:726546/1‑86 (MQ=255)
                                                                                                                                                               ggT‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAAc                                                                                                                                                                                                                                       <  1:726546/86‑1 (MQ=255)
                                                                                                                                                                     tGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAAccgcc                                                                                                                                                                                                                                   >  1:1243574/1‑85 (MQ=255)
                                                                                                                                                                     tGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAAccgcc                                                                                                                                                                                                                                   <  2:1243574/85‑1 (MQ=255)
                                                                                                                                                                     tGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGCCAGCTATATTCTGGTTAACTTCTATACCGGAGCCGGAAGTTGcc                                                                        >  1:1120308/1‑239 (MQ=255)
                                                                                                                                                                        tttacacacaacaaATGTACAAACGCAAAGGAGGGTTGTT‑GGTGGTTATGTTGTTGGTCCAAAAAA‑TTTTACGTTAACCGCGGCGGATGTGGATGCTTTTGGCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATTGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTCGGAAGTTGCCGGa                                                                     <  2:398801/231‑1 (MQ=255)
                                                                                                                                                                          gaaCAGCAACAAAGCTGCAAACGCCAAGGCGGGTTTCT‑GGTGTTGAGTTTGATGGTTCAAAAGA‑TATTACTTTAACCGCCGCGCATGGGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTCGGAAGTTGCCGGAcc                                                                   <  1:1308401/238‑1 (MQ=255)
                                                                                                                                                                            aCAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATCCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCCCTGCCGCCAGCCAATTTCCGGTTAACTTCTAAACCGGAGGCGGAGATGGCCGGAcccg                                                                 >  2:1308401/1‑238 (MQ=255)
                                                                                                                                                                            aCAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGCGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGTAGGGCAACGGATACA‑TATGCCGTTGCGGATGGTGCCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCACTTGCGACAGCCAGATTATGGTTAACTTCTTAACCGGAGTCGGAAGGTGCCGGAcccc                                                                 >  2:354255/1‑238 (MQ=255)
                                                                                                                                                                                 aacaaAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCCCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAATCGGAAATGGCCGGACCCCGccag                                                            >  2:587220/1‑236 (MQ=255)
                                                                                                                                                                                    tatgttGCAAACGCCAGTGCGGTTTTTG‑TGTTTTGAGTTTGATTGTTCAAAAGA‑TTTTCTTATAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTCGGAAGTTGCCGGACCCTGCAGATGa                                                         <  2:1357283/234‑1 (MQ=255)
                                                                                                                                                                                        ctccAAACGCAAAGGTGGTTTTCT‑GGTGTGGATTTTGATGGTACCAAAGA‑TTTTATTTTAACCGGCGCGCATGTGGCTGGTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTCGGAAGTTGCCGGACCCTGCAGATGAAg                                                       <  1:1144737/234‑1 (MQ=255)
                                                                                                                                                                                        cTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTCGGAAGTTGCCGGACCCTGCAGATGAAgg                                                      >  1:694023/1‑238 (MQ=255)
                                                                                                                                                                                         tgaaaccGCCAAGGGGGGTTTGT‑GGT‑TTGGGTTTTGGGGGTCCCAAGAGTATTCCTTAAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTCGGAAGTTGCCGGACCCTGCAGATGAAGgcg                                                    <  1:587220/233‑1 (MQ=255)
                                                                                                                                                                                         tgaaaaCGCAAAGGGGGGTTTGT‑GGGGTTGAGTTTGATGGTCCAAAAGA‑TTTTCCTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTCGGAAGTTGCCGGACCCTGCAGATGAAGgcg                                                    <  2:424330/236‑1 (MQ=255)
                                                                                                                                                                                         tgaaaaCCCCAAGGCGGGTTTAG‑GGGGTTTGTGTTGATGTTTCCAAAGA‑TATTACTTAAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTCGGAAGTTGCCGGACCCTGCAGATGAAGgcg                                                    <  1:480119/236‑1 (MQ=255)
                                                                                                                                                                                         ggCAACCGCAAAGGCGGTTTTCG‑GGTGTTGAGTTTGATGGTCCCAAAGA‑TTTTCCTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTCGGAAGTTGCCGGACCCTGCAGATGAAGgcg                                                    <  1:511473/238‑1 (MQ=255)
                                                                                                                                                                                           tcatccgccgagtggtgttttgggTTTTGTGTTTTTTGG‑TCCAAAGATTATTATGTAATCCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCTGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGACGGAAGTTGCCGGACCCTGCAGATGAAGgcg                                                    <  2:624766/216‑1 (MQ=255)
                                                                                                                                                                                           cAAACGCAAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGc                                                                                                                                                                                                      <  1:262661/92‑1 (MQ=255)
                                                                                                                                                                                            aaaCGCCAAGATGGGTTTTT‑GGTGTTGAGTTTGATGGTTCCAAATA‑TTTTGCTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTCGGAAGTTGCCGGACCCTGCAGATGAAGGCGCAt                                                 <  1:446536/239‑1 (MQ=255)
                                                                                                                                                                                            aaaCGCAAAGACGGGTTTTG‑GGTTTTGAGTTTGATGGTTCAAAAGA‑TTTTGCTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTCGGAAGTTGCCGGACCCTGCAGATGAAGGCGCAt                                                 <  1:909265/239‑1 (MQ=255)
                                                                                                                                                                                                       tcggtttttgggTGTTTAGTTTGATTGTTCCAAATA‑TTTTACTTTAGCCGCCGGGCATGTGGCTGGTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTCGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAAtg                                       <  2:694023/229‑1 (MQ=255)
                                                                                                                                                                                                           ttttttggtgttgtgttgttgttttCCAAAGT‑TATTCCTTTAGCCGGCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑GATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCGGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTCGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAAtggtgg                                   <  2:206492/216‑1 (MQ=255)
                                                                                                                                                                                                           tttttgggTTTTGTGTTTGATGTTTCAAAAGA‑TATTACTGTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTCGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAAtggtg                                    <  2:1053870/232‑1 (MQ=255)
                                                                                                                                                                                                           tttCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTCGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAAtggtgg                                   >  1:206492/1‑238 (MQ=255)
                                                                                                                                                                                                            tttttgtgtttttgttttgttgTTCCAAATG‑TTTTTTTTGAACCGTCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCCGGTTAACTTCTATACCGGAGTCGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATggtggt                                  <  2:523705/218‑1 (MQ=255)
                                                                                                                                                                                                                 ggTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCCACGGATACA‑TATGCCGATGCGGATGGTGTCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTCGGAAGTTGCCGGCCCCTCAAGTTGAAGGCGCATTACATaaagtggggttggt                              >  2:1047312/1‑227 (MQ=255)
                                                                                                                                                                                                                          tttGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGTGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCAGGCGCTTACAATGTCACCCGCTGTGGCACCAGCTATATTCTGGGTAACTTCTATACCGGGGTCGGAAGTTGCCGGCCCATGCAGATGAAGGCGCATTACAGAAATGGGGGTTTGttatcacgt                      >  2:296976/1‑230 (MQ=255)
                                                                                                                                                                                                                          tttGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTCGGAAGTTGCCGGACCCTGCAg                                                             >  1:1290027/1‑198 (MQ=255)
                                                                                                                                                                                                                                 gtaccAAAGT‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTCGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAaga             <  1:286037/236‑1 (MQ=255)
                                                                                                                                                                                                                                  ttCCAAAGT‑TTTTCGTTTACCCGCCGCGCATGTGGCTGCTTTTGCCAGGAGGGCAACGGATACA‑TAGGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTCGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAagag            <  2:148097/239‑1 (MQ=255)
                                                                                                                                                                                                                                  tccaaaaGA‑TCTTCCTTTAACCGCCGCGCATGGGGCTGGTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTCGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAaga             <  1:570046/234‑1 (MQ=255)
                                                                                                                                                                                                                                    ccAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTCGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAAGAGAc          >  1:799530/1‑239 (MQ=255)
                                                                                                                                                                                                                                          attttcaCTTAAACCGCCGGGCATGTGGGTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCTTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATAGTCTGGTTAACTTCTATACCGGAGTCGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTTGTCTGTTCTACCGTTCTTCAAGAGACGGTTa     <  1:941680/233‑1 (MQ=255)
                                                                                                                                                                                                                                            tatTCCTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTCGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAAGAGACGGTTa     <  1:1338894/237‑1 (MQ=255)
                                                                                                                                                                                                                                             atTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATCTTCTGGTTAACTTCTATACCGGAGTCGGAAGTTGCCGGACCCTGCAGCTGAAGGCGCATTACAGAAACGGTGGTCTGTTCTACCGTTCTTCAAGCGACGGTTATgg  >  2:619011/1‑239 (MQ=255)
                                                                                                                                                                                                                                             atTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTCGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAAGAGACGGTTATgg  >  1:633259/1‑239 (MQ=255)
                                                                                                                                                                                                                                             atTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTCGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAAGAGACGGTTATgg  >  1:1120370/1‑239 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                            
TGGTGCATTATGGCGTAATGATGGCGCTAAAACTTACCTGCTGCTTACCAATCAAGGTGATGTTTATGGTGGCTGGAATACATTAAGACCGTTT‑GCTATTGATAACGCAACCGGCGAACT‑GGTTATTGGAACCAAACTGT‑CCGC‑AAGTCTGAAC‑GGT‑AATGCATTAACAGCAACAAAGCTGCAAACGCCAAGACGGGTTTCT‑GGTGTTGAGTTTGATGGTTCCAAAGA‑TATTACTTTAACCGCCGCGCATGTGGCTGCTTTTGCCAGAAGGGCAACGGATACA‑TATGCCGATGCGGATGGTGGCGTTCCATGGAATGCCGAATCTGGCGCTTACAATGTCACCCGCTCTGGCGACAGCTATATTCTGGTTAACTTCTATACCGGAGTCGGAAGTTGCCGGACCCTGCAGATGAAGGCGCATTACAGAAATGGTGGTCTGTTCTACCGTTCTTCAAGAGACGGTTATGG  >  NC_000913/1431235‑1431702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A