Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A15 F18 I0 R1 55 72.5 2455354 98.2% 2411157 146.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,870,339 G→A 38.4% G134S (GGC→AGC)  iap → aminopeptidase in alkaline phosphatase isozyme conversion

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,870,3390GA38.4% 64.0 / 76.5 85G134S (GGC→AGC) iapaminopeptidase in alkaline phosphatase isozyme conversion
Reads supporting (aligned to +/- strand):  ref base G (27/25);  new base A (13/19);  total (40/45)
Fisher's exact test for biased strand distribution p-value = 3.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTACCGAGTATGGTATTCGATTTGTGGCGACCAGCGGCGAAGAGGAAGGGAAATTAGGCGCT  >  CP009273/2870192‑2870485
                                                                                                                                                   |                                                                                                                                                  
gcccgtGATAATCGTAAGCGCTGGCATAGCGGGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGACGCAGATCATCATTATGGCGACTCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAAACTcgg                                                                                                                                                   <  1:177123/149‑1 (MQ=255)
      gATAATCGTAAGAGCTTGCATAACGTGACGGGAAGTACGTTGATTTCCGCTCATGACCGCCACGCGCCGGAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCAGCGGGCt                                                                                                                                             <  1:498489/149‑1 (MQ=255)
      gATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCCcgg                                                                                                                                                   >  2:272891/1‑143 (MQ=255)
       aTAATCGTAAGAGCTGGCATAACGCGACGGNAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGCGCGATGCTGACGCCGATGCCAATCTcagc                                                                                                                                                  <  2:798744/143‑1 (MQ=255)
         aagcgtagaagCTGCCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTcggcgg                                                                                                                                                <  2:108665/134‑1 (MQ=255)
          aTCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAACGCGCCGCAGCAGATCAGCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCggg                                                                                                                                               <  2:980001/143‑1 (MQ=255)
          aTCGTAAGACCCTGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAACGCAGAGCGCCGCCGCAGATTATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCggg                                                                                                                                               <  2:465886/143‑1 (MQ=255)
             gTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGGTTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACACCCCTCTGAGCGGTGCTGACGCCGATGCCAATCTCAGCGGGCCAACGTTa                                                                                                                                      >  1:442826/1‑149 (MQ=255)
                ggagCTGGCATAACGTGACGGGAAGTACGGTAATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACg                                                                                                                                         >  2:831151/2‑143 (MQ=255)
                agagCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGGTCATGAAGGCAAAGGGCCGCAGCAGATGCTCATTCTGGCGCATCTGGATACTTACGCCCGGCTGAGCGATGCTGACGCCGTTGCCAGTGTCGGGGGGCGGagg                                                                                                                                         >  2:252493/1‑141 (MQ=255)
                agagCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCGGACg                                                                                                                                         >  2:1137013/1‑143 (MQ=255)
                     tGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAGGGCAAAGCGCCGCCGAAGATCATCATTGTGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGAGGCCGATGCCAAGCTCGACGGGCCGACGTTACAAgggag                                                                                                                              >  1:1155136/1‑146 (MQ=255)
                       gggtAACGTGACGGGAAGTACGGTGATTGCCGCTCGTGGAGGCAAAGCGCCGCAGCAGTTCATCAGTATGGCGCATCTGGACACTTGCGCCCCGCTGGGCGATGCTGACGCCGATGCCAATCTCAGCGGGCTGACGTTACAAg                                                                                                                                  <  2:461822/140‑1 (MQ=255)
                        cATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCAGCGGGCTGACGTTACAAGGAATGga                                                                                                                           <  1:383455/149‑1 (MQ=255)
                        cATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCAGATGAGTGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGga                                                                                                                           <  1:897296/149‑1 (MQ=255)
                         aTAACGTGACGGGAAGTACGGTGATTGTCGCTCATGAAGGCAAAGCGCCGCAGCAGAACATCATTATGGCGCATCTGGATACTTAAGCCCCGCTGAGCGATGCTGCCGCCGATGCCAAACTCAGCGGGCTGACGTTACAAgaa                                                                                                                                >  2:292475/1‑141 (MQ=255)
                           aaCGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGgatga                                                                                                                        <  1:1059822/149‑1 (MQ=255)
                              gtgtggggAAGTAGGGTGATTGCCGATCACGAAGGCAAAGCCCCGCAGAAGACGATCATTAGGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGTCGCCGATGCCGATCTCGGCGGGCTGACGTTACAAGGAATGga                                                                                                                           <  2:787479/138‑1 (MQ=255)
                              gTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGTGGGCGCGGCGCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATaa                                                                                                                     <  1:619670/149‑1 (MQ=255)
                               tGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAc                                                                                                                    <  1:785538/149‑1 (MQ=255)
                                    ggAAGTACGGTGTTTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTAGGGCGCATCTGGATACTGACGCCCCGCTGAGCGATGCTGACGCCCAGGTCTAGCTCAGCGGGCTGACGTTACAAGGAATGGATGATaa                                                                                                                     <  2:295083/143‑1 (MQ=255)
                                    ggAAGTACGGTGATTGCCGCTTATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGACACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCAGCGGGCTGACGTTACAAGGAATGGATGATaa                                                                                                                     <  2:322451/143‑1 (MQ=255)
                                      aaGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAg                                                                                                             >  1:1246617/1‑149 (MQ=255)
                                            ggTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGGTGCTGACGCCGATGCCCATCTCGGCGGGCTGACGGTCCAAGGAATGGAGGATAACGCCGCAGGATTAg                                                                                                       >  1:1197828/1‑149 (MQ=255)
                                             gTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCAGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAgg                                                                                                      >  1:836186/1‑149 (MQ=255)
                                                  ttccGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGGATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAg                                                                                                       <  2:963940/141‑1 (MQ=255)
                                                    ccGCCCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTTGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATg                                                                                               <  1:549472/149‑1 (MQ=255)
                                                     cGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAgggg                                                                                                    >  2:653688/1‑141 (MQ=255)
                                                       cTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCGGGTGTAGGTGTc                                                                                                  >  2:1036513/1‑143 (MQ=255)
                                                       cTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTc                                                                                                  >  2:1036546/1‑143 (MQ=255)
                                                          aTGAAGGCCAAGCGCCGCAGCAGATCATCATGATGGCGCATCTGGATACTTACGCCCCGCTGTGCGATGCGGACGCCGATGCCAAGCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATg                                                                                               <  2:1068400/143‑1 (MQ=255)
                                                          aTGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCAGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATg                                                                                               >  2:253890/1‑143 (MQ=255)
                                                            gAAGGCAAAGCGCCGCCGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAAtt                                                                                       <  1:1039342/149‑1 (MQ=255)
                                                              aGGCAAGGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTgg                                                                                     <  1:316701/149‑1 (MQ=255)
                                                              aGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCGGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTGGGTGTCATGCTgg                                                                                           >  2:371683/1‑143 (MQ=255)
                                                               ggCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCAGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGa                                                                                          >  2:915622/1‑143 (MQ=255)
                                                               ggCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGAGATGCTGACGCCGATGCCAATCTCAGCGGGCTGACGTTACAAGGCAGGGCTGATAACGCCGCAGGGTTAGGTGGCATGCCGGa                                                                                          >  2:86533/1‑143 (MQ=255)
                                                                gCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGaa                                                                                         >  2:648544/1‑143 (MQ=255)
                                                                gCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCa                                                                                   >  1:581904/1‑149 (MQ=255)
                                                                 cAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCACGCTGGAATTGGCAg                                                                                  >  1:751213/1‑149 (MQ=255)
                                                                 cAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCAGCGGGCGGACGTTACAAGGAATGGATGATAACGCCGCAGGTTGAGGGGTCAGGCTGGAATTGGGAg                                                                                  >  1:15624/1‑149 (MQ=255)
                                                                   aaGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCAGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGaa                                                                                <  1:1039139/149‑1 (MQ=255)
                                                                    aGCGCCGCCGCAGATCATCATTATGGCGGATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTgg                                                                                     <  2:634063/143‑1 (MQ=255)
                                                                    aGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCAGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAAc                                                                               <  1:473486/149‑1 (MQ=255)
                                                                    aGCGCCGCAGCACATCATCATTATGGCGCATCTGGGTATTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAAc                                                                               <  1:211122/149‑1 (MQ=255)
                                                                        ccgcAGCAGATCATCATTATGGCGCATCTGGATACTTCCGCCCCGCTGAGCGGTGCTGCCGCCGATGCCAATCTCGGCGGCCTGACGTTACAAGGCATGGATGAGAACGCCGCACGTTTAGGTGGCATGGTGGAATTGGCaca                                                                                 >  2:1003033/1‑141 (MQ=255)
                                                                            agcagATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCAGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGc                                                                             <  2:932415/143‑1 (MQ=255)
                                                                              cagAACATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCAGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCt                                                                           <  2:95193/143‑1 (MQ=255)
                                                                                    atcatTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGaaaaa                                                                     >  2:1124985/1‑143 (MQ=255)
                                                                                     tcatTATGGTGCATCTGGATACTTACGCCCCGCGGAGCGATGCTGACGCCGATGCCAATCTCAGCGGGCTGACGTTACAAGTAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCCGCCCGCCGGAAGAAt                                                                    <  2:296360/143‑1 (MQ=255)
                                                                                     tcatTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCAGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAAt                                                                    <  2:283603/143‑1 (MQ=255)
                                                                                      catcatGGCGCATCTGGACACTTACGTCCCGGTGAGCCATGGTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAAtg                                                                   <  2:698625/143‑2 (MQ=255)
                                                                                       atTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATAc                                                                  >  2:818223/1‑143 (MQ=255)
                                                                                       atTATGGCGCACCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGACTGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTAc                                                            <  1:757089/149‑1 (MQ=255)
                                                                                           tGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTACCGAg                                                        >  1:1272522/1‑149 (MQ=255)
                                                                                            ggCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCAGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTACCGAGt                                                       <  1:116604/149‑1 (MQ=255)
                                                                                              cgcATCTGGCTACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCAGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTACCGAGTAt                                                     <  1:177291/149‑1 (MQ=255)
                                                                                              cgcATCGGCATCCTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATACCCCCGCCGGTCTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTAcc                                                           <  2:1046382/143‑1 (MQ=255)
                                                                                                cggggcgtaagttACGCCACGCTGAGCGATGCTGAAGAATTTGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGccgaccga                                                         >  2:1052319/12‑143 (MQ=255)
                                                                                                  tCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCAGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAACAATACGCCTACCGAGt                                                       >  2:606340/1‑143 (MQ=255)
                                                                                                    tGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGTAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTACCGAGTATGGTAtt                                               <  1:445127/149‑1 (MQ=255)
                                                                                                     ggATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCAGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTACCGAGTATg                                                    <  2:538529/143‑1 (MQ=255)
                                                                                                       agaCTCACGCCCCTCTGAGCGATGCTGACGGCGATGCCAATCTCAGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATCCTGGAATTGGCAGAACGCCTGAAAAATACGCCTACCGAGTATGGTATTCGa                                            <  1:935959/147‑1 (MQ=255)
                                                                                                          cTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTACAGAGCATGGTAtt                                               >  2:52872/1‑143 (MQ=255)
                                                                                                            tACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATGCGCCTACCGAGTATGGTATTCGATTtgt                                       <  1:848634/149‑1 (MQ=255)
                                                                                                             aCGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTACCGAGTATGGTATTCGATTtgtg                                      <  1:1011138/149‑1 (MQ=255)
                                                                                                              ccccccGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTACCGAGTATGGTATTCGATTTGTgg                                     <  1:257898/147‑1 (MQ=255)
                                                                                                               gCCCCGATGGGCGGCGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGGTAACGCCGCAGGTTAAGGTGCCATGTTGGTATTGGCAGCACGCCTGAAAAATACGCCTACCGAGTATGGTATTCGAtt                                          <  2:560664/143‑1 (MQ=255)
                                                                                                                 cccGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGGTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTCCCGAGTATGGTATTCGATTtg                                        >  2:584451/1‑143 (MQ=255)
                                                                                                                 cccGCTGAGCGATGCTGACGCCGATGCCAATCTCAGCGGGCTGCCGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGCCATGCTGGAATTGGCAGAACGCCTGAACAATACACCTACAGAGAATGGTATTCGAttcg                                        >  2:69521/1‑141 (MQ=255)
                                                                                                                 cccGCTGAGCGATGCTGACGCCGATGCCAATCTCAGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTCCCGCGTATGGTATTCGCTTtg                                        >  2:426291/1‑143 (MQ=255)
                                                                                                                  ccGCTGAGCGATGCTGACGCCGATGCCAATCTCAGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTACCGAGTATGGTATTCGATTTGTGGCGAc                                 <  1:1205914/149‑1 (MQ=255)
                                                                                                                   cGCTGAGCGATGCTGACGCCGATGCCAATCTCAGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTACCGAGTATGGTATTCGATTtgtg                                      <  2:345029/143‑1 (MQ=255)
                                                                                                                       gAGCGATGCTGACGCCGATGCCAAGCTCCGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTACCGAGTATGGTATTCGATTTGTGGCGa                                  <  2:1043923/143‑1 (MQ=255)
                                                                                                                          cGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTACCGAGTATGGTATTCGATTTGTGGCGACCa                               >  2:597056/1‑143 (MQ=255)
                                                                                                                            aTGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTACCGAGTATGGTATTCGATTTGTGGCGACCAGCGGCGaa                       <  1:895453/149‑1 (MQ=255)
                                                                                                                            aTGCTGACGCCGATGCCAATCTCAGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGGTTAGGTGACATGCTGAAATTGTCAGAACGCACGAACCAAATTTCGACCCCGGCAGGTGATCGATTTGTGCGGACCAGCGGCGaa                       >  1:618320/1‑149 (MQ=255)
                                                                                                                             tGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTACCGAGTATGGTATTCGATTTGTGGCGACCAGCGGCGGag                      >  1:385080/1‑149 (MQ=255)
                                                                                                                             tGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTACCAAGTATGGTATTCGATTGTTGGCGACCAgcg                            >  2:188205/1‑143 (MQ=255)
                                                                                                                               cTGACCCCGGCGCTAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTACCGAGTATGGTATTCGATTTGTGGCGACCAgcggc                          <  2:1034603/143‑1 (MQ=255)
                                                                                                                                tGACGCCGATGCCAATCTCAGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTACCGAGTATGGTATTCCATCTGTGGCGACCAgcggcg                         >  2:178833/1‑143 (MQ=255)
                                                                                                                                        aggCCAATCTCAGCGGGCTGACGGTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTACCGAGTATGGTATTCGATTTGTGGCGACCAGCGGCGAAGAGGaa                 <  2:973090/141‑1 (MQ=255)
                                                                                                                                        aTGCCAATCTCAGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTACCGAGTATGGTATTCGATTTGTGGCGACCAGCGGCGAAGAGGAAGGGaaa           <  1:1042609/149‑1 (MQ=255)
                                                                                                                                        aTGCCAACCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTACCGAGTATGGTATTCGATTTGTGGCGACCAGCGGCGAAGAGGaa                 <  2:174388/143‑1 (MQ=255)
                                                                                                                                          gCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAACTACGCCTACCGAGTATGGTATTCGATTTGTGGCGACCAGCGGCGAAGAGGAAgg               >  2:414901/1‑143 (MQ=255)
                                                                                                                                          gCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGATTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTACCGAGTATGGTATTCGATTTGTGGCGACCAGCGGCGAAGAGGAAGGGAAAtt         >  1:579368/1‑149 (MQ=255)
                                                                                                                                            cAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTACCGAGTATGGTATTCGATTTGTGGCGACCAGCGGCGAAGAGAAAGGGAAATTAg       >  1:765753/1‑149 (MQ=255)
                                                                                                                                             aaTCTCAGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTACCGAGTATGGTATTCGATTTGTGGCGACCAGCGGCGAAGAGGAAGGGAAATTAgg      >  1:410508/1‑149 (MQ=255)
                                                                                                                                                 tcGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTACCGAGTATGGTATTCGATTTGTGGCGACCAGCGGCGAAGAGGAAGGGAAATTAGGCGCt  >  1:1078766/1‑149 (MQ=255)
                                                                                                                                                   ggcggGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTACCGAGTATGGTATTCGGTTTGTGGCGACCAGCGGCGAAGAGGAAGGGCAATTAgg      >  2:832425/1‑143 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCCCGCGATAATCGTAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAATACGCCTACCGAGTATGGTATTCGATTTGTGGCGACCAGCGGCGAAGAGGAAGGGAAATTAGGCGCT  >  CP009273/2870192‑2870485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A