Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A15 F18 I40 R1	11	54.2	1757834	98.4%	1729708	146.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	3,204,161	G→A	E8K (GAA→AAA)	rpsU →	30S ribosomal subunit protein S21

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,204,161	0	G	A	100.0%	136.6 / NA	40	E8K (GAA→AAA)	rpsU	30S ribosomal subunit protein S21
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/18); total (22/18)

CCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGT  >  CP009273/3204017‑3204304

ccTTTAAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAaaaa                                                                                                                                             <  1:254399/149‑1 (MQ=255)

 cTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAAc                                                                                                                                            >  1:202763/1‑149 (MQ=255)

 cTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAAc                                                                                                                                            >  1:374106/1‑149 (MQ=255)

  tttCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGGAGTAAAATTCCGCACCATTCTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACg                                                                                                                                           >  1:812870/1‑149 (MQ=255)

  tttCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACg                                                                                                                                           >  1:758303/1‑149 (MQ=255)

      aaaagaGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAACTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAaaaa                                                                                                                                             <  2:653875/143‑1 (MQ=255)

          aaGGCCGGGGTGCTTTACAAAGCAGCAGAAATGGCAGTAAAATTCCGCACCAGTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTc                                                                                                                                   <  1:12447/149‑1 (MQ=255)

              ccGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACg                                                                                                                               <  1:527204/149‑1 (MQ=255)

              ccGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCAGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTAGACg                                                                                                                               >  1:132709/1‑149 (MQ=255)

                     gCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGt                                                                                                                              <  2:184286/143‑1 (MQ=255)

                     gCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGt                                                                                                                              <  2:335445/143‑1 (MQ=255)

                           cAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCg                                                                                                                  >  1:729541/1‑149 (MQ=255)

                            aaaGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGGTCTg                                                                                                                       >  2:660148/1‑143 (MQ=255)

                             aaGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGc                                                                                                                      <  2:692854/143‑1 (MQ=255)

                                   cagcaATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCt                                                                                                                >  2:168259/1‑143 (MQ=255)

                                     gcaATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTTGCGTTGATGCCAGCGGCAAACCGAATGAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTc                                                                                                              >  2:736457/1‑143 (MQ=255)

                                               tAAAATTCCGCACCATTTTGCAATAAGCTGGCGTTGATGCCAGCGGCCAACCGAATTAATCAAAGGTGAGAGGCACCTGCCGGTAATTAAAGTACGTACAACCGAGCCCTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGaaa                                                                                              >  1:870548/1‑149 (MQ=255)

                                               tAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGaaa                                                                                              <  1:798605/149‑1 (MQ=255)

                                                  aaTTCCCCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCg                                                                                                 <  2:630570/143‑1 (MQ=255)

                                                     tCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAg                                                                                        >  1:211568/1‑149 (MQ=255)

                                                       cGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAACGCAGgt                                                                                      >  1:683581/1‑149 (MQ=255)

                                                                    aaTAAGCTGGCGTTGACGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAg                                                                         <  1:278205/149‑1 (MQ=255)

                                                                       aaGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACTTGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTtc                                                                      <  1:724014/149‑1 (MQ=255)

                                                                        aGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTtcg                                                                     >  1:83695/1‑149 (MQ=255)

                                                                           tGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCACGTTACGCGTACCTGCGAAAAAGCAGGTGTTATGGCGGAAGt                                                                        >  2:375521/1‑143 (MQ=255)

                                                                             gCGTTGATGCAAGCGGCAAGCCGAATTAATCAAGGGTGAGAGGCACATGCCGGTAATTAAAGTCCCTAAAAACGAGCAGTTCGACGTAGCTCTGCGTCGCTTCAAGCAAAAAAAAAAAAAAGCAGGTGTTCTGGCGGAAGTtc                                                                      <  2:745969/143‑1 (MQ=255)

                                                                                 tGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAACACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGa                                                            >  1:579627/1‑149 (MQ=255)

                                                                                     ggcAGCGGCAAACCGAATTAATCAAGGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGAAAAAAAAAAAAAGCAGGTGTTCTGGCGGAAGTTcgtcgtcgt                                                              <  2:118300/141‑1 (MQ=255)

                                                                                      ccAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCt                                                       >  1:200710/1‑149 (MQ=255)

                                                                                      ccAGCGGCAAAACGAATTAATCAAAGGTCAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCt                                                       <  1:4256/149‑1 (MQ=255)

                                                                                       cAGCGGCAAACCGAAGTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGa                                                            <  2:512148/143‑1 (MQ=255)

                                                                                                 ccGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAACCCg                                            >  1:578865/1‑149 (MQ=255)

                                                                                                        aaTCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGa                                           <  2:389084/143‑1 (MQ=255)

                                                                                                                     agGCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTAAgcgcgc                        >  1:401936/1‑149 (MQ=255)

                                                                                                                       gCACATGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCGCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTAAgc                            >  2:664977/1‑143 (MQ=255)

                                                                                                                            tGCCGGTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGCGAGTTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCt                 <  1:582148/149‑1 (MQ=255)

                                                                                                                                ggTAATTAAAGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTaaaa                   >  2:171973/1‑142 (MQ=255)

                                                                                                                                  caaTTAAAGTACGGAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCa           <  1:39586/148‑1 (MQ=255)

                                                                                                                                       aaaGTACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGaa      >  1:782197/1‑149 (MQ=255)

                                                                                                                                           tACGTAAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGt  >  1:605572/1‑149 (MQ=255)

CCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGT  >  CP009273/3204017‑3204304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A