Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A18 F25 I0 R1
|
17 |
19.2 |
625642 |
96.1% |
601241 |
148.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
CP009273 |
2,725,094 |
A→G |
21.0% |
G813G (GGT→GGC) |
clpB ← |
protein disaggregation chaperone |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP009273 | 2,725,094 | 0 | A | G | 21.0%
| 30.3
/ 2.8
| 19 | G813G (GGT→GGC) | clpB | protein disaggregation chaperone |
| Reads supporting (aligned to +/- strand): ref base A (6/9); new base G (2/2); total (8/11) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 |
TCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGACGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGTTTGTACAGACGTTTCAACTGAATCTGCGCAATCGAGGCAATGTGCTGTT > CP009273/2724955‑2725230
|
tCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTTCACGTTTCAGAGGACGTGCACCATAGAcg > 2:86280/1‑148 (MQ=255)
tCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGACGTGCACCATAGAcc > 2:157271/1‑149 (MQ=255)
ggACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCCCGTTTCAGAGGACGTGCGCCGTAGACCGGATCGTaa > 2:36383/1‑149 (MQ=255)
ggACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGACGTGCGCCATAGACCGGATCGTaa > 2:36428/1‑149 (MQ=255)
ggcGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGACGTGCACCATAGACCGGATCGTAAc < 1:46330/147‑1 (MQ=255)
aTCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGACGTGCACCATAGACCGGATCGTAACCGTTCTCGCt < 2:159656/149‑1 (MQ=255)
gTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGCATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGTACGTTTCAGAGGACGTGCACCATAGACCGGATCGTAACCGTTCTCGCTcagca < 1:172043/149‑1 (MQ=255)
aGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGACGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTGAGCGCCTCGTc > 2:288470/1‑149 (MQ=255)
gCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGACGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCATTTTCCGCGCCTCGTCAg > 2:103261/1‑149 (MQ=255)
ttACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGCATTGCACGTTTCAGAGGACGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGAt < 2:12229/149‑1 (MQ=255)
aCCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGTGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGACGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGAttt < 2:199172/149‑1 (MQ=255)
ccagccagTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTCTCAGAGGACGTGCACCATAGACCGGCTCGTAACCGTTCTCGCCCAGCAGTTTCCGCGCCTCGTCAGAAATGTGGATTTCATACCCACGttcttc < 1:275741/149‑1 (MQ=255)
ctattgtgttggCCGGCTGGATTGCGTTCTGCTGCTGAATTGCACGGTTCAGAGGACGTGCACCATAGACCGGACCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTTAGAAATGTGGATTTCAGAACCACGTTCTTCCAGACGt < 1:256163/138‑1 (MQ=255)
tGCTGGGCCCGCGGGTGTTCGATCTGCTGCTGAATTGCACGTTTCAGCGGACGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGTTTGTa < 1:80506/149‑1 (MQ=255)
gggTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGACGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGTTTGTACAGACGTTTCaa < 2:216101/149‑1 (MQ=255)
ttttCGATCTGCTGCTGAATTGCACGTTTCAGAGGACGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGACATGTGGATTTCATAACCACGTTCCTCCAGACGTTTGTACAGACGTTTCAAcgg > 1:283030/1‑147 (MQ=255)
gATCTGCTGCTGAATTGCACGTTTCAGAGGACGTGCACCATAGACCGGATCGTAACCGTTCTCGCGCAGCAGTTTCAGCGCCTCGCCAGAAATGTGGAATTCATTACCACGTTCTTCCAGACGTTTGTAAAGCCGTTCCACCTGactgt > 1:128782/1‑145 (MQ=255)
cAGAGGACGTGCGCCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGTTTGTACAGACGTTTCAACTGAATCTGCGCAATCGAGGCAATGTGCTGtt < 1:36428/149‑1 (MQ=255)
cAGAGGACGTGCGCCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGTTTGTACAGACGTTTCAACTGAATCTGCGCAATCGAGGCAATGTGCTGtt < 1:36383/149‑1 (MQ=255)
|
TCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAATCACTTTACCCGGAACCAATTCACCAGACAGTATTTGCTGTGCCAGCGGGTTTTCGATCTGCTGCTGAATTGCACGTTTCAGAGGACGTGCACCATAGACCGGATCGTAACCGTTCTCGCTCAGCAGTTTCAGCGCCTCGTCAGAAATGTGGATTTCATAACCACGTTCTTCCAGACGTTTGTACAGACGTTTCAACTGAATCTGCGCAATCGAGGCAATGTGCTGTT > CP009273/2724955‑2725230
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
