Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F32 I0 R1
|
15 |
16.5 |
534746 |
98.2% |
525120 |
146.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
CP009273 |
3,708,519 |
G→A |
19.1% |
N412N (AAC→AAT) |
bisC ← |
biotin sulfoxide reductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP009273 | 3,708,519 | 0 | G | A | 19.1%
| 30.4
/ 4.8
| 21 | N412N (AAC→AAT) | bisC | biotin sulfoxide reductase |
| Reads supporting (aligned to +/- strand): ref base G (9/8); new base A (2/2); total (11/10) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.64e-02 |
TTCAGAGATCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTGGTATCCTGATGATGAGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCTGCCCTGCATGGAAGAGAGCACCGCAGAACGCCGCGTGGGGTTAC > CP009273/3708390‑3708655
|
ttCAGAGATCACCGCCAGCTCCGGTTTTTGCCAGGCAAGAATCAAGCGAATGGTAACCTGATGATGAGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGCAATGTCAGTTCATACCGTTGTGGTGATATGCGccac > 2:3607/1‑149 (MQ=255)
gagaTCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTGGTATCCTGATGATGAGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGTAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAgg < 2:105869/149‑1 (MQ=255)
aTCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTGATATCCTGATGATGAGAAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCGTAGCGTTGATATGCGCCCCCAggggt > 2:44291/1‑147 (MQ=255)
caccccccggCTCCGGTGTTTGCGAGGCACGGAGCAGCTGATTGGTATCCTGATGATGAGTAAAGTTGGCACCGCCCGGCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAg < 1:146926/135‑1 (MQ=255)
caccacCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTGGTATCCTGATGATGAGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCCgg > 1:166816/1‑144 (MQ=255)
tGCCAGGCACGGATCAGGCGATTGGTATCCTGATGATGAGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCATTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCg > 2:278062/1‑149 (MQ=255)
tGCCAGGCACGGATCAGGCGATTGGTATCCTGATGATGAGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCATTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCg > 2:278024/1‑149 (MQ=255)
ggCGATTGGTATCCTGATGATGAGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAGGGATTTTa < 2:136098/149‑1 (MQ=255)
cGATTGGTATCCTGATGATGAGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAGGGATTTTATc < 2:184379/149‑1 (MQ=255)
gATTGGTATCCTGATGATGAGTAACGTTGGCACCACCCGCCCACCAGATAAAACAAATAACCGGGAAATGTCGATTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGAAACAGGGATTTTGTcg > 2:78993/1‑148 (MQ=255)
ggTAGCCTGAGGATGAGTAAAGTTGGCACCTCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGTTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAGGGATTTTATCCACCg < 2:116085/149‑1 (MQ=255)
tCCTGATGATGAGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATc > 2:224368/1‑149 (MQ=255)
tgaGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCAGGAAAAGTCGGAACATACCGTAGAGTTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCAcc > 2:148385/1‑149 (MQ=255)
gTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGGAATGTCGGGTCATACCGTTGTGTTGATATTCGCCACCAGGGTTGTACAGTGCTTTAACAATGCGGGCAACAGGGAGTTTATGCACCGCATCGCAGCCa > 1:184856/1‑144 (MQ=255)
taGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCATTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCAccc < 1:254572/143‑1 (MQ=255)
aaGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCATTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCAccc < 1:254609/144‑1 (MQ=255)
aGTTGGCACCGCGCGCCGATCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAGGGATTTTATTCACCGCATGGCAGTCACCCg < 1:82464/144‑1 (MQ=255)
accaGATAAAACGAATATCCGGGAAATGTCGGTTCATACCGTAGTATTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCTGCCCTGCATGGAagag > 2:140731/1‑149 (MQ=255)
aaaaCGAATATCCTGAAGATGAGTGTTCGTACCGTTGTGGCGATATGCTTCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCTGCCCTGCATGGAagagag < 1:92485/144‑1 (MQ=255)
ccGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCTGCCCTGCATGGAAGAGAGCACCGCAGAAcgccgc > 2:148762/1‑149 (MQ=255)
tCGGTTCATACCGTTGTGTTGATATGCTCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGTAAGGTGTCCTGCATGGAAGAGAGCACCGCAGAACGCCGCGTGGGGTTac < 2:46295/149‑1 (MQ=255)
|
TTCAGAGATCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTGGTATCCTGATGATGAGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCTGCCCTGCATGGAAGAGAGCACCGCAGAACGCCGCGTGGGGTTAC > CP009273/3708390‑3708655
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
