Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A20 F18 I40 R1	7	47.3	1551690	96.0%	1489622	148.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	3,960,035	C→A	R87S (CGC→AGC)	rho →	transcription termination factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,960,035	0	C	A	98.2%	166.2 / NA	57	R87S (CGC→AGC)	rho	transcription termination factor
Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (28/28); minor base G (0/1); total (28/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAG  >  CP009273/3959889‑3960178

tcttGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATcagc                                                                                                                                               <  2:245238/146‑1 (MQ=255)

tCCTGAAGCAGTACGAAAAGAGTGGCGAAGATATCTTGGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATcagc                                                                                                                                               <  2:245523/149‑1 (MQ=255)

      agcagcACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTc                                                                                                                                         <  1:738918/149‑1 (MQ=255)

            aCGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGCATAGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTc                                                                                                                                   >  2:605236/1‑149 (MQ=255)

                    ggTGGCGAAGATATCTTTGGTGCTGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTgg                                                                                                                           <  1:173319/148‑1 (MQ=255)

                     ttGGCGAGCATATCTTGGTTGATGGCGTACGGGAGATATTGCCGGAAGAAGTGGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCGAATCAGCCGTTTCAACCTCCGCACTGGt                                                                                                                          <  2:177508/148‑1 (MQ=255)

                       ggCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGa                                                                                                                        <  2:598850/149‑1 (MQ=255)

                        gCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGGCGGTTCAACCTCCGCACTGGTGag                                                                                                                       >  1:419204/1‑148 (MQ=255)

                        gCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAAACAGCTCCAACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGAt                                                                                                                       >  2:214046/1‑149 (MQ=255)

                          gAAGGTTTCTTTGGTGGTGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGTGCTCTCAGCCGTTTCAACCTCCGCACTGGTGATAc                                                                                                                     <  1:168392/149‑1 (MQ=255)

                           aaGAGATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATAcc                                                                                                                    <  2:229446/149‑1 (MQ=255)

                              atatCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCACCGCACTGGTGATACCAtc                                                                                                                 >  2:677606/1‑149 (MQ=255)

                                      ggTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCTCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGTACTGGTGATACCATCTCTGGTaa                                                                                                         <  2:407501/149‑1 (MQ=255)

                                       tgtatgggcgaCTGGAGGTATTGCAGGATGGATTTTGTTTTCTCGTTTCCGCAGACAGCTCCTACCTCTTCGGTCCTGATGACCTCTACGTTTCTCCTAGCTAAATCATCCGTTTCAACCTCCGCACTGGTGATACCCTCTTTGGTAAg                                                                                                        <  1:9400/139‑1 (MQ=255)

                                            ggCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCACCCTCCGCCCTGGTGATACCATCTCTGGTAAGATTcg                                                                                                   <  1:721476/149‑1 (MQ=255)

                                                 aCTGGAGATATTTCAGGATGGATTTTGTTTCCACCGTTCCGCATATAGCTCCTACCTCGCCGGTCCTAATGACAACTACGCTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTcgcccgc                                                                                              <  2:245471/149‑1 (MQ=255)

                                                  cTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTcgcccgcc                                                                                             >  1:55981/1‑149 (MQ=255)

                                                      agaTATTGCAGGATGGATTTGGTTTCCTCCGCTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCTAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGaaa                                                                                         <  2:520701/149‑1 (MQ=255)

                                                            tGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAGGCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGt                                                                                   <  2:340236/149‑1 (MQ=255)

                                                            tGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCCCTGGTAAGATTCGCCCGCCGCAAGAAGGt                                                                                   >  1:260226/1‑149 (MQ=255)

                                                             gCAGGATGGATTTGGTTTCCTCCGTTGCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTg                                                                                  >  1:767416/1‑149 (MQ=255)

                                                             gCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTg                                                                                  >  2:73461/1‑149 (MQ=255)

                                                              cAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGATGa                                                                                 >  2:104936/1‑149 (MQ=255)

                                                                    tgatTTGGTGTCTCCCGTGCCTCAGACAGCTCGTCCCTCGCCGGTCCTCATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTa                                                                           <  1:592229/148‑1 (MQ=255)

                                                                     gatTTGGTTTTCTCCGTGCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACATTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTAt                                                                          >  1:573187/1‑149 (MQ=255)

                                                                      atTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCCCCCGCCGAAAGAAGGTGAACGCTAtt                                                                         >  1:398738/1‑149 (MQ=255)

                                                                      atTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGCTTCCCCTAGCCAAATCAGACGTTTCAACCTCCACACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTAtt                                                                         >  2:86981/1‑149 (MQ=255)

                                                                           gTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGCTACCATCCCTGGTAAGATTCGCCCGCCGAAAGAGGGGGCACGCCATTTTccg                                                                    >  1:12183/1‑149 (MQ=255)

                                                                           gTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGCGAACGCTATTTTgcg                                                                    >  1:121944/1‑149 (MQ=255)

                                                                            tttCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGACGGTGAACGCTATTTTgcgc                                                                   >  1:135786/1‑149 (MQ=255)

                                                                            tttCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGATGATACCATCTCTGGTAAGATTCGCCCGCGGAAAGAAGGTGAACGCTATTTTgcgc                                                                   >  2:461324/1‑149 (MQ=255)

                                                                              tcctccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCgctg                                                                 <  2:546381/149‑1 (MQ=255)

                                                                                ctccGTTCCGCAGACAGCTCCTACATCGCCGGTCCTGATGACATCTACGTTTCCACTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTTCTTTGCgctgct                                                               >  1:176288/1‑149 (MQ=255)

                                                                                 tccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGctgctg                                                              >  2:593124/1‑149 (MQ=255)

                                                                                    gtgccGCAGGCAGCGCCGACCGCGCGGGTCCTGATGAGCTCTACGTTGCCCCTAGACAGATCGGCGGTTTCAGCCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCGCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGaaa                                                           <  1:30946/146‑1 (MQ=255)

                                                                                    gTTCCGCAGCCAGCTCCTCCCTCGCCGGTCCTGATGACATTTACGTGTCCCTTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGaaa                                                           <  2:405459/149‑1 (MQ=255)

                                                                                    gTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGaaa                                                           <  2:190387/149‑1 (MQ=255)

                                                                                       ccGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGtt                                                        >  1:294911/1‑149 (MQ=255)

                                                                                        gccagacatacccTACCTCGCCGGTCCTGATGACATCTACGCTTCCCCGAGTCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTTTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTa                                                       <  1:13014/138‑1 (MQ=255)

                                                                                        cGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCCCCGCCCTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAAGTGAACGCTATTTTACGCTGCTGAAAGTTa                                                       >  2:10692/1‑149 (MQ=255)

                                                                                                 cTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGCTTCGCCCGCGGCTAGCTGGTGTAGGCTTTTTTGCGCTGCTGAAAGTAAAGGAAGTTa                                              >  2:234721/1‑149 (MQ=255)

                                                                                                  tCCTACCTCGCCGGTCCTGATGACAGCTACGTTTCCCCTAGCCACATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCACTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTaa                                             <  2:478112/149‑1 (MQ=255)

                                                                                                   ccTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGGTTCAACCTCCGCACGGGTGATACCATATCTGGAAAGAGTCGCACGACGAAAGAATTTGAACGCTATTTTGCGCTGCTAAAAGTTAACGAAGTTAAc                                            <  2:568499/149‑1 (MQ=255)

                                                                                                    cTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCACCTTCCCCACCAGAGATACCATCACTGGTAAGATTCGCCCCCCCAAAGAACATTAACACTATTTCGCGCTACTTAAAAGTAACGACATTAACt                                           >  2:451084/1‑149 (MQ=255)

                                                                                                     tACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTCCGCCCCTGAACGTTAACGAAGTTAACtc                                          >  2:306156/1‑148 (MQ=255)

                                                                                                      aCCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTc                                         <  2:55981/149‑1 (MQ=255)

                                                                                                       ccTCGCCGGTCCTGATGACTTCTACTTTTCCCCTAGCCATATCAGCTGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAActtcgttaactttc                                        <  2:279265/149‑14 (MQ=255)

                                                                                                         tcgtcgGTCCTGTGTGCATCGACGTTTCCGCTAGCCAAACCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGAc                                      <  1:271107/149‑1 (MQ=255)

                                                                                                                       gaCTTCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGc                        <  2:661972/149‑1 (MQ=255)

                                                                                                                        aCATCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGcc                       <  2:213713/149‑1 (MQ=255)

                                                                                                                          agcTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCGCCGCACTGGTGATACCATCTCTCGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTACCGCAGTTAACTTCGACAAACCTGAAAACGCCCg                     <  2:378896/147‑1 (MQ=255)

                                                                                                                          aTCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCGGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCg                     >  1:716102/1‑149 (MQ=255)

                                                                                                                           tCTACGTTTCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGc                    >  1:709460/1‑149 (MQ=255)

                                                                                                                                 tttCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCAACTCAGGTACGATCCGCCCGCCGAAAGAAGGTGCACGCTATTATGCGCTGCTGAAAGTTAACGCAGTTAACTCCGGAAAACCTGAAGACGCGCGcaggcaa              >  1:601017/1‑144 (MQ=255)

                                                                                                                                  ttCCCCTAGCCAAATCAGCCGTTTCAACCTCCGCACGGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCAGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACaaaa             <  2:532195/149‑1 (MQ=255)

                                                                                                                                       cTAGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGCTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCctc        >  1:595761/1‑149 (MQ=255)

                                                                                                                                         aGCCAAATCAGCCGTTTCAACCTCCGCACTGGTGATACCAGCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCtt      <  1:686864/149‑1 (MQ=255)

                                                                                                                                          gCCAAATCAGCCGGTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCttt     <  2:317978/149‑1 (MQ=255)

                                                                                                                                             aaaTCAGCCGTTTCAACCTCCGCACTGGTGATACCCTCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCAGAAAACGCCCACAACAAAATCCTCTTTgag  >  2:12236/1‑149 (MQ=255)

TCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAG  >  CP009273/3959889‑3960178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A