breseq  version 0.32.0b  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 445,409 C→T W230* (TGG→TAG)  cyoB ← cytochrome o ubiquinol oxidase subunit I
RA 468,126 Δ1 bp coding (73/339 nt) glnK → nitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB
RA 1,449,090 C→A Q303K (CAA→AAA)  paaA → ring 1,2‑phenylacetyl‑CoA epoxidase subunit
RA 2,719,426 T→C intergenic (‑321/+2) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
JC 3,270,632 (TTCAACA)2→3 coding (272/336 nt) prlF → antitoxin of the SohA(PrlF)‑YhaV toxin‑antitoxin system
RA 3,960,035 C→A R87S (CGC→AGC)  rho → transcription termination factor
RA 4,390,235 T→A I44N (ATC→AAC)  hfq → global sRNA chaperone; HF‑I, host factor for RNA phage Q beta replication

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 345710 346916 1207 14 [13] [13] 15 prpC prpC
* * ÷ CP009273 748602 749945 1344 14 [13] [13] 14 gltA gltA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 360104 =NA (NA)71 (1.510) 58/296 0.0 NA intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 = 1391495NA (NA)33 (0.700) 24/296 1.1 100% intergenic (+146/‑127) insH1/ynaJ IS5 transposase and trans‑activator/DUF2534 family putative inner membrane protein
?CP009273 = 1972751 0 (0.000)intergenic (‑297/‑483) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
* ? CP009273 1972748 =2 (0.040)47 (1.000) 30/294 0.6 95.9% intergenic (‑294/‑486) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
?CP009273 = 2283592 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? CP009273 2269338 =0 (0.000)36 (0.760) 32/296 0.5 100% coding (587/1026 nt) yejE microcin C transporter YejABEF, permease subunit; ABC family
?CP009273 = 3577555 NA (NA)intergenic (+15/‑1) insB1/yrhA IS1 transposase B/pseudogene, interrupted by IS1E
* ? CP009273 = 22693462 (0.040)53 (1.120) 46/296 0.1 96.4% coding (595/1026 nt) yejE microcin C transporter YejABEF, permease subunit; ABC family
?CP009273 3576788 = NA (NA)intergenic (+10/‑55) yrhA/insA pseudogene, interrupted by IS1E/IS1 repressor TnpA
* ? CP009273 2455823 =2 (0.040)43 (0.910) 36/296 0.3 95.6% coding (1039/1341 nt) fadL long‑chain fatty acid outer membrane transporter
?CP009273 3576788 = NA (NA)intergenic (+10/‑55) yrhA/insA pseudogene, interrupted by IS1E/IS1 repressor TnpA
* ? CP009273 = 24558312 (0.040)41 (0.870) 34/296 0.4 95.3% coding (1047/1341 nt) fadL long‑chain fatty acid outer membrane transporter
?CP009273 = 3577555 NA (NA)intergenic (+15/‑1) insB1/yrhA IS1 transposase B/pseudogene, interrupted by IS1E