Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F20 I0 R1 52 51.1 1736300 96.9% 1682474 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,176,709 Δ1 bp 6.3% intergenic (+27/+463) zupT → / ← ribB zinc transporter/3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,176,7090A. 6.3% 182.6 / 7.6 63intergenic (+27/+463)zupT/ribBzinc transporter/3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (36/22);  new base . (2/2);  total (39/24)
Fisher's exact test for biased strand distribution p-value = 6.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-01

ATGAATTAATGCCGCTCGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGTTTCAAATGCAGGAAA  >  CP009273/3176561‑3176857
                                                                                                                                                     |                                                                                                                                                    
atggATTGATGCCGCTCGACACAGGGCTTGATTCTTACAGTCACCCCAGCTTTGGCGGTCTATGTGGAATTTCTGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCa                                                                                                                                                      <  1:387246/149‑1 (MQ=255)
 tgaATTAATGCCGCTCGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCAt                                                                                                                                                     >  2:44341/1‑149 (MQ=255)
 tgaATTAATGCCGCTCGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCAt                                                                                                                                                     <  1:504015/149‑1 (MQ=255)
   aaTTAATGCCGCTGGCCAAAGGGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGTATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATgg                                                                                                                                                   >  2:615383/1‑149 (MQ=255)
     ttAACGCCGCTCGCCACAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGAc                                                                                                                                                 <  2:164696/149‑1 (MQ=255)
       aaTGCCGCTCGCCAAAGAGATTGACCCTAATAATACCCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACaa                                                                                                                                               <  2:842575/149‑1 (MQ=255)
        acgCCGCTCGACAAAGAGATTGACTCTAATAATCACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAAc                                                                                                                                              <  2:723273/147‑1 (MQ=255)
            cGCTCGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTGTGTGGAATGTCAGTGATGGGAGTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGCAGTTGTCAA‑GCGCATGTACaaagtct                                                                                                                                          >  2:228513/1‑144 (MQ=255)
              cTCGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAAAGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGc                                                                                                                                        >  1:556438/1‑149 (MQ=255)
                    aaaGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCtt                                                                                                                                  >  2:89749/1‑149 (MQ=255)
                      agagATTGACCCTAATAATACCCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACTGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCa                                                                                                                                <  2:338698/149‑1 (MQ=255)
                       gctatggACGCTAATAACACCCCGCGCTATGGCGTACGATGTTGAATGTCAGTGATGGGAGTCAGTTGAGTGCTGCCACAAACGGCGGGAATTGGTTAAAAACAACGTCCGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCAc                                                                                                                               <  1:753770/143‑1 (MQ=255)
                        agaTTGACCCTACTACTCTCCCTAGCTATGGCGTACTATGTGGAAGGTCAGTGATGGGATTCAGTTTAGTTCTGCTACTAACGGCGGGAATTGGTTAAAAACAACAGCAGAAGTTGTCCATGCGC‑TGGACAACTTCTgatgttgttttt                                                                                                                              <  1:684527/149‑12 (MQ=25)
                        agaTTGACCCTAATGATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACAGCAGAAGTTGTCCATGCGC‑TGGACAACTTCTgatgttgttttt                                                                                                                              <  1:717541/149‑12 (MQ=37)
                          aTTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCTGCTTCACGaa                                                                                                                            >  2:329187/1‑149 (MQ=255)
                             gACCTTAATGATAACCCCAGCTAGGGCCTACTATGTGGAATGTCAGTGATGGGAGTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGc                                                                                                                         <  1:146718/149‑1 (MQ=255)
                                ccTAATAGTAACGCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTTTCCA‑GCGCATGGACAACTTCTACCAGCATCACGaaacactt                                                                                                                      >  1:58630/1‑144 (MQ=255)
                                ccTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTTCTACACACGGCGCGTATCTGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTACCAGCTTCCCGCAAGCCtt                                                                                                                      >  1:742277/1‑149 (MQ=255)
                                ccTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCtt                                                                                                                      >  1:160460/1‑149 (MQ=255)
                                ccTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGATTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCACCTGCACTAAATCCtt                                                                                                                      >  1:564585/1‑149 (MQ=255)
                                       ataaCCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCCGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTCATTCTag                                                                                                               >  1:432028/1‑148 (MQ=255)
                                        taaCCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTAcc                                                                                                              <  2:380205/149‑1 (MQ=255)
                                                  tATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTg                                                                                                    >  2:740729/1‑149 (MQ=255)
                                                   aTGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGt                                                                                                   <  2:780980/149‑1 (MQ=255)
                                                     gccgtcccgtgttGAATGTCGGTGATGTGATTCAGCTTAGTGGTGCTAGCACCGGAGGGAATTGGTTAAAATCTACATCAGAAGTGGTCCA‑GCGCATGGACAACTTCTGCCAGCGTCACGAAAGCCTTATTCTACCTTTTCGGCTGtct                                                                                                 <  1:422740/140‑1 (MQ=255)
                                                         tACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtctcttc                                                                                             >  2:768969/1‑149 (MQ=255)
                                                          aCTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTTTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAATGCCTTATTCTACCTTTTCGGCTGtctcttcc                                                                                            <  1:635359/149‑2 (MQ=255)
                                                          aCTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtctcttct                                                                                            <  2:83905/149‑1 (MQ=255)
                                                           cTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtctcttctc                                                                                           >  2:537936/1‑149 (MQ=255)
                                                              tgtgGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTAATTCTAACTTTTCGGCTGTCTCTTCTCTCg                                                                                        >  2:583226/1‑149 (MQ=255)
                                                                        cAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTaa                                                                              >  1:130778/1‑149 (MQ=255)
                                                                         aGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAg                                                                             >  2:695025/1‑149 (MQ=255)
                                                                             aTGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTAtt                                                                         >  2:735032/1‑149 (MQ=255)
                                                                             aTGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTAtt                                                                         <  1:291755/149‑1 (MQ=255)
                                                                             aTGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTAtt                                                                         >  2:39092/1‑149 (MQ=255)
                                                                              tGGGAGTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGTTTAACAACAACATCAGAAGTTGTCCA‑GCGCATGGACTTCCTATCCTACCGTCACTGGAGCCTTTTTCTACCTTTTCGGCTCTCTCTCCTCTCGTACTGTTTACGTAttt                                                                        <  1:401114/149‑1 (MQ=255)
                                                                                ggATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTAACTTTTCTGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGt                                                                      >  2:377421/1‑149 (MQ=255)
                                                                                ggAATCAGCTTAGGTCTGCTACAAACGGCGGGAATTGGTTAGAAACAAGATGAGGAGTTGTCCA‑GAGCATAGACAACTTCTACCAGCCTCACCCAAGCCGTATTCTACATTTTCTGCTGTCTCCTTTCTCGCACTGTTTAAGTCttttt                                                                      >  1:61557/1‑147 (MQ=255)
                                                                                   ttCCGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTCAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTCCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTcc                                                                   <  2:566920/149‑1 (MQ=255)
                                                                                    tCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcg                                                                  >  2:826576/1‑149 (MQ=255)
                                                                                     cAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcgc                                                                 <  1:259498/149‑1 (MQ=255)
                                                                                            gTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTACCGCGCTTCaa                                                          >  2:59719/1‑149 (MQ=255)
                                                                                                 gctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGCCAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTAATTGTTCCGCGCTTCAAACAGt                                                     >  1:41120/1‑149 (MQ=255)
                                                                                                   tACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGc                                                   <  1:810688/149‑1 (MQ=255)
                                                                                                     cAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGt                                                 <  2:675023/149‑1 (MQ=255)
                                                                                                      aaaCGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGtc                                                >  2:199769/1‑149 (MQ=255)
                                                                                                       aaCGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGtct                                               <  1:740729/149‑1 (MQ=255)
                                                                                                           gcggGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGt                                           >  2:105929/1‑149 (MQ=255)
                                                                                                                     tttAAAAACAACATCAGAAGGTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGTCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTCAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGaaa                                 <  1:195584/148‑1 (MQ=255)
                                                                                                                       tAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGGAACg                               >  1:393939/1‑149 (MQ=255)
                                                                                                                          aaaCAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGGGTCTCTGTCCTGGCGGATCGccc                            >  1:709025/1‑149 (MQ=255)
                                                                                                                           aagaaaaTCAGAGGTTGTCCA‑GCGTGGGGAGATATGCTGAAAGGTTGAGGGAGGCCTTATTGTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTGTAAGTATTTGTTCTGGGCTTCAAGCAGTGCGTTTCTGTCCTGGCGATACGccgg                           >  1:79718/7‑147 (MQ=255)
                                                                                                                              gctggcAGAAGTTGTCCATGCGC‑TGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACa                        >  2:404475/6‑149 (MQ=39)
                                                                                                                                  tCAGAGGTTGTCCC‑GCGCATGGACCACTTCTGCCAGCTTCCCGAATGCCTTCTCCTACCTCTCCGCCTGTCTCTTCTCTCGTACTGTTTACGTATTTGTTCCGCGCTGCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCTCGACAATaa                    <  1:651996/149‑1 (MQ=255)
                                                                                                                                   cAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCGTCAAGCAGTGCGTCTCTGTCCTGGCGAGACGCCCGACAGTAAc                   >  1:290990/1‑149 (MQ=255)
                                                                                                                                       aGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGGAACGGCCGACACTAACGttt               >  1:607058/1‑149 (MQ=255)
                                                                                                                                       aGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAAGAACGttt               >  2:862783/1‑149 (MQ=255)
                                                                                                                                        gTTGTCCATGCGC‑TGGACAACTTCTGCCAGCTTCACGCAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGttta              >  2:316636/1‑148 (MQ=255)
                                                                                                                                            tCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAACCTTATTCCACCCTTTCGGCTGTCTCTCCTCACGTACTGGTTAAGTCGTTGCTCCGCGCATGAAGCAGTCCGTCGCTGTCCTGCCGAAACGCCCGACAATAACGTTGCacag          >  1:733819/1‑146 (MQ=255)
                                                                                                                                             ccA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGCCAATAACGTTTCAAATg         >  1:73929/1‑149 (MQ=255)
                                                                                                                                             ccA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGTTTCAAATg         >  1:805534/1‑149 (MQ=255)
                                                                                                                                              cA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGTTTCAAATGc        >  2:317253/1‑149 (MQ=255)
                                                                                                                                                 gcgcATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGTTTCAAATGCAg      >  1:562635/1‑149 (MQ=255)
                                                                                                                                                 gcgcATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCAACAATAACGTTTCAAATGCAg      <  2:834881/149‑1 (MQ=255)
                                                                                                                                                     aTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGTTTCAAATGCAGGaaa  <  2:187902/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ATGAATTAATGCCGCTCGCCAAAGAGATTGACCCTAATAATAACCCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCA‑GCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGCAGTGCGTCTCTGTCCTGGCGAAACGCCCGACAATAACGTTTCAAATGCAGGAAA  >  CP009273/3176561‑3176857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A