Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A16 F20 I0 R1
|
52 |
51.1 |
1736300 |
96.9% |
1682474 |
149.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
CP009273 |
3,706,647 |
G→T |
28.7% |
V66L (GTG→TTG) |
tag → |
3‑methyl‑adenine DNA glycosylase I, constitutive |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP009273 | 3,706,647 | 0 | G | T | 28.7%
| 35.3
/ 22.2
| 35 | V66L (GTG→TTG) | tag | 3‑methyl‑adenine DNA glycosylase I, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (14/11); new base T (8/2); total (22/13) |
| Fisher's exact test for biased strand distribution p-value = 2.59e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.27e-01 |
TGATAATGAGTGGGGCGTGCCTGAAACTGACAGTAAAAAACTGTTCGAAATGATCTGCCTTGAAGGGCAGCAGGCTGGATTATCGTGGATCACCGTCCTCAAAAAACGCGAAAACTATCGCGCCTGCTTTCATCAGTTCGATCCGGTGAAGGTCGCAGCAATGCAGGAAGAGGATGTCGAAAGACTGGTACAGGACGCCGGGATTATCCGCCATCGAGGGAAAATTCAGGCAATTATTGGTAATGCGCGGGCGTACCTGCAAATGGAACAGAACGGCGAACCGTTTGTCGACTT > CP009273/3706502‑3706795
|
tGATAATGAGTGGGGCGTGCCTGAAACTGACAGTAAAAAACTGTTCGAAATGATCTGCCTTGAAGGGCAGCAGGCTGGATTATCGTGGATCACCGTCCTCAAAAAACGCGAAAACTATCGCGCCTGCTTTCATCAGTTCGATCCGGTGa > 2:658552/1‑149 (MQ=255)
tGATAATGAGTGGGGCGTGCCTGAAACTGACAGTAAAAAACTGTTCGAAATGATCTGCCTTGAAGGGCAGCAGGCTGGATTATCGTGGATCACCGTCCTCAAAAAACGAGTGGCCTTTCGCGCCTGCTTTCATCAGTTCGATCCGTTGa < 2:425195/149‑1 (MQ=255)
ggggCGTGCCTGAAACTGACAGTAAAAAACTGTTCGAAATGATCTGCCTTGAAGGGCAGCAGGCTGGATTATCAAGGCTCCCCATCCACAAAAAACGCGAAAACTCTCGCGCCTGCTTTCATCAGTTCGATCCGGTGAAGGTCgcagca > 1:95258/1‑149 (MQ=255)
gggCGTGCCTGAAACTGACAGTAAAAAACTGTTCGAAATGATCTGCCTTGAAGGGCAGCAGGCTGGATTATCGTGGATCACCGTCCTCAAAAAACGCGAAAACTATCGCGCCTGCTTTCATCAGTTCGATCCGGTGAAGGTCGCAGCaa > 2:104722/1‑149 (MQ=255)
ggCGTGCCTGAAACTGACAGTAAAAAACTGTTCGAAATGATCTGCCTTGAAGGGCAGCAGGCTGGATTATCGTGGATCACCGTCCTCAAAAAACGCGAAAACTATAGCGCCTGCTTTCATCAGTTCGATCCGTGGAAGGTCGAAGCAAt > 2:303712/1‑149 (MQ=255)
aaaCTGACAGTAAAAAACTGTTCGAAATGATCTGCCTTGAAGGGCAGCAGGCTGGATTATCGTGGATCACCGTCCTCAAAAAACGCGAAAACTATCGCGCCTGCTTTCATCAGTTCGATCCGGTGAAGGTCGCAGCAATGCAGGAagag < 2:609265/149‑1 (MQ=255)
gACAGTAAAAAACTGTTCGAAATGATCTGCCTTGAAGGGCAGCAGGCTGGATTATCGTGGATCACCGTCCTCAAAAAACGCGAAAACTATCGCGCCTGCTTTCATCAGTTCGATCCGGTGAAGGTCGCAGCAATGCAGGAAGAGGATGt > 1:687464/1‑149 (MQ=255)
aaaaCTGTTCGAAATGATCTGCCTTGAAGGGCAGCAGGCTGGATTATCGTGGATCACCGTCCTCAAAAAACGCGAAAACTATCGCGCCTGCTTTCATCAGTTCGATCCGGTGAAGGTCGCAGCAATGCAGGAAGAGGATGTCGCAAGAc > 1:237624/1‑149 (MQ=255)
tGTTCGAAATGATCTGCCTTGAAGGGCAGCAGGCTGGATTATCGTGGATCACCGTCCTCAAAAAACGCGAAAACTATCGCGCCTGCTTTCATCAGTTCGATCCGGGGAAGGTCGCAGCAATGCAGGAAGAGGATGTCGAAAGACTGGTa > 1:834162/1‑149 (MQ=255)
tGTTCGAAATGATCTGCCGTGAAGGGCAGCAGGCTGGATTATCGGGGATCACCGTCCTCAAAAAACGCGAAAACTATCGCGCCTGCTTTCATCAGTTCGATCCGGTGAAGGTCGCAGCAATGCAGGAAGAGGATGTCGAAAGACTGGTa < 2:99176/149‑1 (MQ=255)
ttCGAAATGATCTGCCTTGAAGGACAGCCGGCTGAATTATCGTGGATCACCGTACTCAAAAAACGCGAAAACTATCGCGCCTGCTTTCATCAGTTCGATCCGGTGAAGGTCGCAGCAATGCAGGAAGAGGATGTCGAAAGACTGGTCCa > 2:479778/1‑149 (MQ=255)
aaaTGATCTGCCTTGAAGGGCAGCAGGCTGGATTATCGTGGATCACCGTCCTCAAAAAACGCGAAAACTATCGCGCCTGCTTTCATCGGTTCGATCCGGTGCAGGTCGCACCACTGCAGGGAAAGGATGTCGAAAGACTGGTACCGGAc > 2:199777/1‑149 (MQ=255)
cTGCCTTGAAGGGCAGCAGGCTGGATTATCGTGGATCACCGTCCTCAAAAAACGCGAAAACTATCGCGCCTGCTTTCATCAGTTCGATCCGTTGAAGGTCGCAGCAATGCAGGAAGAGGATGTCGAAAGACTGGTACAGGACGCCGGGa > 1:715061/1‑149 (MQ=255)
cTGCCTTGAAGGGCAGCAGGCTGGATTATCGTGGATCACCGTCCTCAAAAAACGCGAAAACTATCGCGCCTGCTTTCATCAGTTCGATCCGGTGAAGGTCGCAGCAATGCAGGAAGAGGATGTCGAAAGACTGGTACAGGACGCCGGGa < 1:104722/149‑1 (MQ=255)
cTTGAAGGGCAGCAGGCTGGATTATCGTGGATCACCGTCCGCGAAAAACGAGAAAAGTATCGCGCCTGCTTTCATCAGTTCGATCAGGTGGAGGACGCAGCAATGCAGGAAGAGGGTGCCGAAAGACTGGTACAGGCGGCCGGGATTaa > 1:423117/1‑148 (MQ=255)
aGGGCAGCAGGCTGGATTATCGTGGATCAGCGTCCTCAAAAAACGCGAAAACTATCGCGCCTGCTTTCATCAGTTCGATCCGTTGAAGGACGCAGCAATGCAGGAAGAGGATGTCGAAAGACTGGTACAGGCCGCCGGGCTTATCCGcc < 1:640650/149‑1 (MQ=255)
gcagGCTGGATTATCGTGGATCACCGTCCTCAAAAAACGCGAAAACTATCGCGCCTGCTTTCATCAGTTCGATCCGGTGAAGGTCGCAGCAATGCAGGCAGAGGATGTCGAAAGACTGGTCCAGGACGCCCGGATTCTCCGCCATCTAg > 2:799884/1‑149 (MQ=255)
ttATCGTGGTTCACCGTCCTCAAAAAACGCGAAAACTATCGCGCCTGCTTTCATCAGGTCGATCCGGTGAAGGTCGCAGCAATGCAGGAAGAGGATGTCGAAAGACTGGTACAGGACGCCGGGATTATCCGCCATCGAGGGAAAATTCa < 1:374937/149‑1 (MQ=255)
tagcGTGGATCACCGTCCTCAAAAAACGCGAAAACTATCGCGCCTGCTGTCATCAGTTCGATCCGGTGAAGGTCGCAGCAATGCAGGAAGAGGATGTCGAAAGACTGGTACAGGACGCCGGGATTATCCGCTATCGAGGGAAAATTCAg < 1:510229/146‑1 (MQ=255)
tATCGTGGATGACGCTCCGTAAAAAACGCGAAAACTATCGCGCCGGCGTTCCTCAGTTCGATCCGGTGAAGGTCGCAGCAATGCAGGAAGAGGAGGTCGAAAGACTGGTACAGGACGCCGGGATTATCCGCCATCGAGGGAAAATTCAg < 1:509670/149‑1 (MQ=255)
tCGTGGATCACCGTCCTCAAAAAACGCGAAAACTATCGCGCCTGCTTTCATCAGTTCGATCCGTTGAAGGTCGCAGCAATGCAGGAAGAGGATGTCGAAAGACTGGTACAGGACGCCGGGATTATCCGCCATCGAGGGAAAATTCAGGc > 1:660564/1‑149 (MQ=255)
aTCACCGTCCTCAAAAAACGCGAAAACTATCGCGCCTGCTTTCATCAGTTCGATCCGGTGAAGGTCGCAGCAATGCAGGAAGAGGATGTCGAAAGACTGGTACAGGACGCCGGGATTATCCGCCATCGCGGGAAAATTCAGGCAattat > 1:374965/1‑149 (MQ=255)
aTCACCGTCCTCAAAAAACGCGAAAACTATCGCGCCTGCTTTCATCAGTTCGATCCGGTGAAGGTCGCAGCAATGCAGGAAGAGGATGTCGAAAGACTGGTACAGGACGCCGGGATTATCCGCCATCGAGGGAAAATTCAGGCAattat > 2:787893/1‑149 (MQ=255)
tCAAAAAACGCGAAAACTATCGCGCCTGCTTTCATCAGTTCGATCCGTTGAAGGTCGCAGCAATGCAGGAAGAGGATGTCGAAAGACTGGTACAGGACGCCGGGATTATCCGCCATCGAGGGAAAATTCAGGCAATTATTGGTAATgcg > 2:280260/1‑149 (MQ=255)
gcgAAAACTATCGCGCCTGCTTTCATCAGTTCGATCCGGTGAAGGTCGCAGCAATGCAGGAAGAGGATGTCGAAAGACTGGTACAGGACGCCGGGATTATCCGCCATCGAGGGAAAATTCAGGCAATTATTGGTAATGCGCGGGCGTAc < 2:788081/149‑1 (MQ=255)
cgAAAACTATCGCGCCTGCTTTCATCAGTTCGATCCGTTGAAGGTCGCAGCAATGCAGGAAGAGGATGTCGAAAGACTGGTACAGGACGCCGGGCTTATCCGCCATCGAGGGAAAATTCAGGCAATTCTTGGTAATGCGCGGGctcacc > 1:72319/1‑144 (MQ=255)
gAAAACTATCGCGCCTGCTTTCATCAGTTCGATCCGTTGAAGGTCGCAGCAATGCAGGAAGAGGATGTCGAAAGACTGGTACAGGACGCCGGGATTATCCGCCATCGAGGGAAAATTCAGGCAATTATTGGTAATGCGCGGGCGTACCt > 1:70660/1‑149 (MQ=255)
cgcgCCTGCTTTCATCAGTTCGATCCGGTGAAGGTCGCAGCAATGCAGGAAGAGGATGTCGAAAGACTGGTACAGGACGCCGGGATTATCCGCCATCGAGGGAAAATGCAGGCAATTATTGGTAATGCGGGGGCGTACCTGCAAATGGa > 1:656139/1‑149 (MQ=255)
gcgcCTGCTTTCATCAGTTCGATCCGGTGAAGGTCGCAGCAATGCAGGAAGAGGATGTCGAAAGACTGGTACAGGACGCCGGGATTATCCGCCATCGAGGGAAAATTCAGGCAATTATTGGTAATGCGCGGGCGTACCTGCAAATGGaa < 2:687464/149‑1 (MQ=255)
gcgcCTGCTTTCATCAGGTCGATCCGGTGAAGGTCGCAGCAATGCAGGAAGAGGATGTCGAAAGACTGGTACAGGACGCCGGGATTATCCGCCATCGAGGGAAAATTCAGGCAATTATTGGTAATGCGCGGGCGTACCTGCAAATGGaa < 1:436624/149‑1 (MQ=255)
ccTGCTTTCATCAGTTCGATCCGTTGAAGGTCGCAGCAATGCAGGAAGAGGATGTCGAAAGACTGGTACAGGACGCCGGGATTATCCGCCATCGAGGGAAAATTCAGGCAATTATTGGTAATGCGCGGGCGTACCTGCAAATGGAACAg > 2:731915/1‑149 (MQ=255)
ccTGCTTTCATCAGTTCGATCCGTTGAAGGTCGCAGCAATGCAGGAAGAGGATGTCGAAAGACTGGTACAGGACGCCGGGATTATCCGCCATCGAGGGAAAATTCAGGCAATAATTGGTAATGCGCGGGCGTACCTCCAAATGGAACAg > 2:172654/1‑149 (MQ=255)
gTTCGATCCGGTGAAGGTCGCAGCAATGCAGGAAGAGGATGTCGAAAGACTGGTACAGGACGCCGGGATTATCCGCCATCGAGGGAAAATTCAGGCAATTATTGGTAATGCGCGGGCGTACCTGCAAATGGAACAGAACGGCGAACCGt < 2:75889/149‑1 (MQ=255)
tCGATCCGGTGAAGGTCGCAGCAATGCAGGAAGAGGATGTCGAAAGACTGGTACAGGACGCCGGGATTATCCGCCATCGAGGGAAAATTCAGGCAATTATTGGTAATGCGCGGGCGTACCTGCAAATGGAACAGAACGGCGAACCGttt < 2:320705/149‑1 (MQ=255)
tCGATCCGGTGAAGGTCGCAGCAATGCAGGAAGAGGATGTCGAAAGACTGGTACAGGACGCCGGGATTATCCGCCATCGAGGGAAAATTCAGGCAATTATTGGTAATGCGCGGGCGTACCTGCAAATGGAACAGAACGGCGAACCGttt > 2:662495/1‑149 (MQ=255)
ggTGAAGGTCGCAGCAATGCAGGAAGAGGATGTCGAAAGACTGGTACAGGACGCCGGGATTATCCGCCATCGAGGGAAAATTCAGGCAATTATTGGTAATGCGCGGGCGTACCTGCAAATGGAACAGAACGGCGAACCGTTTGTCGGCt > 2:375775/1‑149 (MQ=255)
gTGAAGGTCGCAGCAATGAAGGAAGAGGATGTCGAAAGACTGGTACAGGACGCCGGGAGTATCCGCCATCGAGGGAAAATTCAGGCAATTATTGGTAATGCGCGGGCGTACCTGCAAATGGAACAGAACGGCGAACCGTTTGTCGACtt < 1:418072/149‑1 (MQ=255)
|
TGATAATGAGTGGGGCGTGCCTGAAACTGACAGTAAAAAACTGTTCGAAATGATCTGCCTTGAAGGGCAGCAGGCTGGATTATCGTGGATCACCGTCCTCAAAAAACGCGAAAACTATCGCGCCTGCTTTCATCAGTTCGATCCGGTGAAGGTCGCAGCAATGCAGGAAGAGGATGTCGAAAGACTGGTACAGGACGCCGGGATTATCCGCCATCGAGGGAAAATTCAGGCAATTATTGGTAATGCGCGGGCGTACCTGCAAATGGAACAGAACGGCGAACCGTTTGTCGACTT > CP009273/3706502‑3706795
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
