Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F20 I0 R1	91	60.0	1338914	94.3%	1262595	140.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,021,817	C→T	8.3%	V139I (GTT→ATT)	USA300HOU_1873 ←	acyl‑CoA hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,021,817	0	C	T	8.3%	203.3 / 6.2	74	V139I (GTT→ATT)	USA300HOU_1873	acyl‑CoA hydrolase
Reads supporting (aligned to +/- strand): ref base C (41/26); new base T (3/3); total (44/30)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01

CCTTAACAATCTGTCTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAATTCTAATTTGAACGACCACTT  >  CP000730/2021677‑2021954

ccTTAACAATCTGTCTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCCATTGTTTGTTTAATTTCCATGCGACGTGATTTTCTTCGTTCAACAAGTTGTGGTGCTGTATCATAAAACCATTTTTCAAc                                                                                                                                           >  1:300905/1‑141 (MQ=255)

ccTTAACAATCTGTCTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGTTGCTGTACCATAAAACCATTTTTCAAc                                                                                                                                           >  2:401374/1‑141 (MQ=255)

ccTTAACAATCTGTCTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAAc                                                                                                                                           >  2:130467/1‑141 (MQ=255)

 cTTAACAATCTGTCTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAAca                                                                                                                                          >  2:90510/1‑141 (MQ=255)

   tAACAATCTGTCTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAAcatc                                                                                                                                        <  2:516808/141‑1 (MQ=255)

    aaCAATCTGTCTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAAcatca                                                                                                                                       <  1:225234/141‑1 (MQ=255)

    aaCAATCTGTCTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAAcatca                                                                                                                                       >  1:568104/1‑141 (MQ=255)

       aaTCTGTCTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATATAACCCTTTTTTAACATCAtct                                                                                                                                    >  2:22058/1‑141 (MQ=255)

           tGTCTTTATATTTTTAGTCCCTAATCTGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGCGCTTTTCTTCGTTCAACTCGTTGCGTTGCTGTAGCATAAAGCCACTTTTCAACATCAgcttctt                                                                                                                                >  2:483929/1‑140 (MQ=255)

                ttATATTTTTAGTCTCTAATATGTTGCACTTGGGATCCCCAGTCAATTGTTTGTTAACTGTGAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAGAACCATTTTTCAACATCATCTTCTGGatat                                                                                                                           >  1:658595/1‑141 (MQ=255)

                ttATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGatat                                                                                                                           >  2:146987/1‑141 (MQ=255)

                ttATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGatat                                                                                                                           <  2:633408/141‑1 (MQ=255)

                 tataTTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGAtata                                                                                                                          <  2:444181/141‑1 (MQ=255)

                      ttttCGTCTCTAATATGTTGCACTTGAGCTAAACATTCAATTGTTTGGTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCa                                                                                                                     <  2:202414/141‑1 (MQ=255)

                      ttttAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCa                                                                                                                     >  1:20160/1‑141 (MQ=255)

                      tttgAGTCTCCAATATGTTGCACTTGAGCTAAATATTCAATTGCTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTGCCACACGTTGTGGTGCTGGATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCa                                                                                                                     <  1:181140/141‑1 (MQ=255)

                       tttAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACAcccg                                                                                                                    >  1:227721/1‑139 (MQ=255)

                         tAGTCAATAATATGTTGCACTTGAGATCAATGTTCAATTGATTGTTTACTTTCAATGCGACGTGCTCTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCACCATCATCTTCTTGATATACACCAGGt                                                                                                                  >  1:490897/1‑141 (MQ=255)

                          aGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTa                                                                                                                 >  1:234633/1‑141 (MQ=255)

                           gTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTAc                                                                                                                >  2:378560/1‑141 (MQ=255)

                            tctcTAATATGTTGCACTTGAGCTAAATATTCACTTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACa                                                                                                               >  1:602797/1‑141 (MQ=255)

                              tctAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATg                                                                                                             <  1:146987/141‑1 (MQ=255)

                              tctAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATg                                                                                                             <  2:20160/141‑1 (MQ=255)

                                tAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGtt                                                                                                           <  1:566778/141‑1 (MQ=255)

                                tAATATGTTGCACTTGAGCTAAATACTCAATTGTTTGTTTACTTCCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCAGGTACATGtt                                                                                                           <  1:166621/141‑1 (MQ=255)

                                     tGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGt                                                                                                      >  2:314511/1‑141 (MQ=255)

                                       ttGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGttt                                                                                                    <  2:258744/141‑1 (MQ=255)

                                           aCTTGAGCTAAATATTCCATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCt                                                                                                <  2:582202/141‑1 (MQ=255)

                                           aCTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCt                                                                                                >  1:523126/1‑141 (MQ=255)

                                               gAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCACCACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTtcat                                                                                            >  1:131965/1‑141 (MQ=255)

                                               gAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCATCATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTtcat                                                                                            <  1:10779/141‑1 (MQ=255)

                                               gAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTtcat                                                                                            <  1:111562/141‑1 (MQ=255)

                                                  cTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTcatcat                                                                                         >  1:34247/1‑141 (MQ=255)

                                                    aaaTATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCt                                                                                       <  1:119016/141‑1 (MQ=255)

                                                        atTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTaaag                                                                                   >  1:589731/1‑139 (MQ=255)

                                                        atTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACg                                                                                   <  2:286847/141‑1 (MQ=255)

                                                         ttCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGc                                                                                  >  1:492280/1‑141 (MQ=255)

                                                           cAATTGTTTGTCTACTTGCAGTGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAGCCATTTTTCGACATCATCTTCTGGGTATACACGAGGTACATGTTTAGGTTTGCCTTCATCATCTAACAcaa                                                                                >  2:6471/1‑141 (MQ=255)

                                                            agttttttgtttAGTTTGAATGCGACTGGCTTTTCTTCGTTCAAAAGTCTCGGGTGCTGTATCATAAAACCGTTGTTCAAGATCATCTTGTGGATATAAGCCATGTACATGTTTAGGTTTGCCGTCATCATCTAACGcaac                                                                               <  2:20719/136‑1 (MQ=255)

                                                            aaTTGTTTGTTTACTTTCAATGCTACGTGCTTTTTCTCTCTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGcaac                                                                               <  2:433733/141‑1 (MQ=255)

                                                             aTTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGcaaca                                                                              <  1:397746/141‑1 (MQ=255)

                                                              ttgtttgtTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGcaacaa                                                                             >  1:558403/1‑141 (MQ=255)

                                                              ttgtttgtTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGcaacaa                                                                             >  1:97010/1‑141 (MQ=255)

                                                                     tttACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTaa                                                                      >  1:212425/1‑141 (MQ=255)

                                                                        aCTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTa                                                                   >  1:359713/1‑141 (MQ=255)

                                                                        aCTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTa                                                                   >  2:22486/1‑141 (MQ=255)

                                                                          tttCAATGGGACGTGCTTTTCTTCGTTCAACCCGTTGTGGTGCTGGATCATAAAACCCTTTTTCAACATCATCTTCTGGATATACGCCAGGTACATGTTTAGGTTTGCCTTCATCATTTAACGCAACAAATGTTAAGTAAc                                                                 <  1:158864/141‑1 (MQ=255)

                                                                             cAATGCGACGTGCTTTGCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCAGGCACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAAATTa                                                              >  1:371437/1‑141 (MQ=255)

                                                                                tGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATg                                                           <  1:40113/141‑1 (MQ=255)

                                                                                  cGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCCTCTAACGCACCAAATGTTAAGTAACTTAATgag                                                         >  2:251740/1‑139 (MQ=255)

                                                                                        gCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTaaa                                                   >  1:256931/1‑141 (MQ=255)

                                                                                         cTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAAt                                                  <  2:476030/141‑1 (MQ=255)

                                                                                         cTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAAt                                                  >  2:576667/1‑141 (MQ=255)

                                                                                         cTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAAt                                                  <  2:602797/141‑1 (MQ=255)

                                                                                          ttttCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCCTCTTCTGGATATACACCAGGTCCATGTTTAGGTTTGCCTTCATCATCTAACGCAACCAATGTTAAGTAACTTAATGCAGCTAAATc                                                 >  2:125497/1‑141 (MQ=255)

                                                                                                       acacGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACTAGGTACATGTTTAGGTTTGCCTTCAGCATCTAACGCAACAAATGTTAAGTAACTTAATGCATCTAAATCATGCTTGttatta                                    <  1:420938/141‑1 (MQ=255)

                                                                                                       acacGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGttatta                                    >  1:663167/1‑141 (MQ=255)

                                                                                                         acGTTGTGGTGCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTaaa                                  >  1:116610/1‑141 (MQ=255)

                                                                                                            ttGTGGTGCTGACTCATAACACCATTTTTCAACATCATCTTCTGGATATACACCAGGTAGATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACATAATACAGCTAAATCATCCTAGTTATTAAATAc                               >  2:374104/1‑141 (MQ=255)

                                                                                                             tgtgGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATAATGCTTGTTATTAAATACa                              >  2:384480/1‑141 (MQ=255)

                                                                                                             tgtgGCGCTGTATCATAAAACCATTTTTCAACATCATCTTATGGATATACACCAGGTACATGTTTATGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAACTAAATCATGCTTGTTATTCAATaga                              >  1:490494/1‑139 (MQ=255)

                                                                                                              gtggtgCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACAt                             <  2:177564/141‑1 (MQ=255)

                                                                                                              gtggtgCTGTATCATAAAACCATTTTTCAATATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACAt                             <  2:177405/141‑1 (MQ=255)

                                                                                                               tggtgCTGTATCATAAAACCATTTTTCAACATCCTCTTCTGGATATACACAAACTACATGTCTATTTTTACCTTCATCATCTAACGCAACAAATGTGAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATc                            <  2:658595/141‑1 (MQ=255)

                                                                                                                  tgCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTc                         >  1:665589/1‑141 (MQ=255)

                                                                                                                  tgCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTc                         >  1:327739/1‑141 (MQ=255)

                                                                                                                  tgCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTc                         >  1:69986/1‑141 (MQ=255)

                                                                                                                    cTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCaa                       <  1:561118/141‑1 (MQ=255)

                                                                                                                     tGTAACATAAAACCATGTTTCCACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAAt                      <  1:270750/141‑1 (MQ=255)

                                                                                                                       tATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAATTc                    >  1:516973/1‑141 (MQ=255)

                                                                                                                       tATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGAACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAATTc                    >  2:193496/1‑141 (MQ=255)

                                                                                                                         tCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAATTCTa                  >  2:182277/1‑141 (MQ=255)

                                                                                                                          cATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAATTCTaa                 >  1:413676/1‑141 (MQ=255)

                                                                                                                              aacccttttttCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAATTCTAATTTg             <  2:25812/135‑1 (MQ=255)

                                                                                                                                aCCATTTTTCTACTTCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCTTCTAACGCAACATATGTTAAGTGGCTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTGAATTCTTATTTgga           >  2:50261/1‑139 (MQ=255)

                                                                                                                                aCCATTTTTCAACATCATCTTCTGGATATACACCAGGTACCTCTTTTGATTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAATTCTAATTTGaa           >  1:628240/1‑141 (MQ=255)

                                                                                                                                aCCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAATTCTAATTTGaa           >  1:3673/1‑141 (MQ=255)

                                                                                                                                    tttttCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCTGCTAAATCATGCTTGTTATTAAATACATCGTCAATTCTAATCTGAACGAc       <  2:492280/141‑1 (MQ=255)

                                                                                                                                        tCAATATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAATTCTAATTTGAACGACCACt   >  1:478341/1‑141 (MQ=255)

                                                                                                                                        tCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAATTCTAATTTGAACGACCACt   <  1:327856/141‑1 (MQ=255)

                                                                                                                                         cAACACCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAATTCTAATTTGAACGACAACtt  >  2:79262/1‑141 (MQ=255)

CCTTAACAATCTGTCTTTATATTTTTAGTCTCTAATATGTTGCACTTGAGCTAAATATTCAATTGTTTGTTTACTTTCAATGCGACGTGCTTTTCTTCGTTCAACACGTTGTGGTGCTGTATCATAAAACCATTTTTCAACATCATCTTCTGGATATACACCAGGTACATGTTTAGGTTTGCCTTCATCATCTAACGCAACAAATGTTAAGTAACTTAATGCAGCTAAATCATGCTTGTTATTAAATACATCGTCAATTCTAATTTGAACGACCACTT  >  CP000730/2021677‑2021954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A