Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A21 F117 I0 R1	36	24.6	540534	94.7%	511885	140.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,486,381	G→T	100%	Q5215K (CAA→AAA)	ebh ←	extracellular matrix binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,486,381	0	G	T	100.0%	63.9 / NA	20	Q5215K (CAA→AAA)	ebh	extracellular matrix binding protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (8/12); total (8/12)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AAGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTGATTAATGATTGCTTTTGCTGCAGTAACTGCACTGTCGTACTCGTTACGATTATTTGGACTTGCGTCAGTGTAGTTTTGACCTGCTTTAATTGTTGCTTCATTCGCAATACTATCTCTTAAGCCTTTCATCGCTGTAT  >  CP000730/1486254‑1486518

aaGTTGTTTTTCGCAGTTGTCTTAGTTTGGGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTTATTAATGATTGCt                                                                                                                              <  2:140896/141‑1 (MQ=255)

 aGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTTATTAATGATTGCtt                                                                                                                             >  1:238407/1‑141 (MQ=255)

                    cTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTTATTAATGATTGCTTTTGCTGCAGTAACTGCACt                                                                                                          <  2:109254/141‑1 (MQ=255)

                                 aaGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTTATTAATGTTTGCTTTTGCTGCAGTAACTGCACTGCCGTACTCGttc                                                                                             >  2:228120/1‑140 (MQ=255)

                                    tttCGCGCAGCATTTTATGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTTATTAATGATTGCTTTTGCTGCAGTAACTGCACTGTCGTACTCGTTACGa                                                                                          <  2:178656/141‑1 (MQ=255)

                                    tttCGCGCACCATTTACTGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTTATTAATGATTGCTTTTGCTGCAGTAACTGCACTGTCGTACTCGTTACGa                                                                                          <  1:70976/141‑1 (MQ=255)

                                        gcgcACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTTATTAATGATTGCTTTTGCTGCAGTAACTGCACTGTCGTACTCGTTACGattat                                                                                      >  2:89831/1‑141 (MQ=255)

                                               aTTTAATGCCTGTTCTTTAGTTTTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTTATTAATGATTGCTTTTGCTGCAGTAACTGCACTGTCGTACTCGTTACGATTATTTGGACt                                                                               <  1:147548/141‑1 (MQ=255)

                                                  tAATGCCTGTTCTTTAGTTGTCCCTTGTGATTTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTTATTAATGATTGCTTTTGCTGCAGTAACTGCACTGTCGTACTCGTTACGATTATTTGGACTTGc                                                                            <  2:5170/141‑1 (MQ=255)

                                                                    tGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTTATTAATGATTGCTTTTGCTGCAGTAACTGCACTGTCGTACTCGTTACGATTATTTGGACTTGCGTCAGTGTAGTTTTGAcc                                                          >  1:52788/1‑141 (MQ=255)

                                                                     ttCACTTGTGATTTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTTATTAATGATTGCTTTTGCTGCAGTAACTGCACTGTCGTACTCGTTACGATTATTTGGACTTGCGTCAGTGTAGTTTTGACCt                                                         <  1:200212/140‑1 (MQ=255)

                                                                     ttCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTTATTAATGATTGCTTTTGCTGCAGTAACTGCACTGTCGTACTCGTTACGATTATTTGGACTTGCGTCAGTGTAGTTTTGACCt                                                         <  1:193338/140‑1 (MQ=255)

                                                                        ccTTGTGATGTTACTTGCGTAATTGTATTTGGTTCCATCGTTGGGTTCGATGTTTTATTAATGATTGCTTTTGCTGCAGTAACTGCACTGTCGTACTCGTTACGATTATTTGGACTTGCGTCAGTGTAGTTTTGACCTGCt                                                      <  2:108989/140‑1 (MQ=255)

                                                                              gATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTTATTAATGATTGCTTTTGCTGCAGTAACTGCACTGTCGTACTCGTTACGATTATTTGGACTTGCGTCGGTGTAGTTTTGACCTGCTTTAAtt                                                >  2:203997/1‑141 (MQ=255)

                                                                               aTGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTTATTAATGATTGCTTTTGCTGCAGTAACTGCACTGTCGTACTCGTTACGATTTTTTGGGCTTTCGTCAGTGTTGTTTTGACCTGCTTTAAttt                                               >  1:208148/1‑140 (MQ=38)

                                                                                  ttACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTTATTAATGATTGCTTTTGCTGCAGTAACTGCACTGTCGTACTCGTTACGATTATTTGGACTTGCGTCAGTGTAGTTTTGACCTGCTTTAAttgttg                                            <  2:238407/141‑1 (MQ=255)

                                                                                                   tttGGTGCCATTGTTGGGTTCGATGTTTTATTAATGATTGCTTTTGCGGCAGTAACTGCACTGTCGTACTCGTTACGATTATTTGGACTTGCGTCAGTGTAGTTTTGACCTGCTTTAATTGTTGCTTCATTCGCAATACTa                           <  2:65840/141‑1 (MQ=255)

                                                                                                            aTCGTTGGGTTCGATGTTTTATTAATGATTGCTTTTGCTGCAGTAACTGCACTGTCGTACTCGTTACGATTATTTGGACTTGCGTCAGTGTAGTTTTGACCTGCTTTAATTGTTGCTTCATTCGCAATACTATCTCTTAAg                  >  1:194848/1‑141 (MQ=255)

                                                                                                                 tGGGTTCGATGTTTTATTAATGATTGCTTTTGCTGCAGTAACTGCACTGTCGTACTCGTTACGATTATTTGGACTTGCGTCAGTGTAGTTTTGACCTGCTTTAATTGTTGCTTCATTCGCAATACTATCTCTTAGTCCttt             >  1:58175/1‑141 (MQ=255)

                                                                                                                    gTTCGATGTTTTATTAATGATTGCTTTTGCTGCAGTAACTGCACTGTCGTACTCGTTACGATTATTTGGACTTGCGTCAGTGTAGTTTTGACCTGCTTTAATTGTTGCTTCATTCGCAATACTATCTCTTAAGCCTTTCAt          <  1:89831/141‑1 (MQ=255)

                                                                                                                            ttttATTAATGATTGCTTTTGCTGCAGTAACTGCACTGTCGTACTCGTTACGATTATTTGGACTTGCGTCAGTGTAGTTTTGACCTGCTTTAATTGTTGCTTCATTCGCAATACTATCTCTTAAGCCTTTCATCGCTGTAt  <  1:21788/141‑1 (MQ=255)

AAGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTGATTAATGATTGCTTTTGCTGCAGTAACTGCACTGTCGTACTCGTTACGATTATTTGGACTTGCGTCAGTGTAGTTTTGACCTGCTTTAATTGTTGCTTCATTCGCAATACTATCTCTTAAGCCTTTCATCGCTGTAT  >  CP000730/1486254‑1486518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A