breseq  version 0.32.1  revision 5f2cb669173e
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 39,378 C→A 100% D586Y (GAT→TAT)  mecA ← penicillin binding protein 2 prime
RA 110,660 T→A 11.8% I173I (ATT→ATA USA300HOU_0109 → staphylococcal tandem lipoprotein
RA 111,512 A→G 19.0% S194S (TCA→TCG USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 111,516 C→A 21.0% Q196K (CAA→AAA)  USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 162,467 A→G 12.1% L63S (TTA→TCA)  USA300HOU_0153 ← ABC superfamily ATP binding cassette transporter, membrane protein
RA 478,502 C→A 100% D342E (GAC→GAA USA300HOU_0457 → NADH dehydrogenase (ubiquinone)
RA 512,643 A→G 53.5% intergenic (+711/‑150) recR → / → rrs1 recombination protein RecR/16S ribosomal RNA
RA 584,121 G→T 100% intergenic (+205/‑67) rplA → / → rplJ ribosomal protein L1/ribosomal protein L10
RA 612,076 A→G 12.8% T130A (ACA→GCA)  sdrC → Ser‑Asp rich fibrinogen/bone sialoprotein‑binding protein SdrC
RA 707,909 A→T 100% M1M (TTG→ATG) † USA300HOU_0653 ← ABC superfamily ATP binding cassette transporter, ABC protein
RA 938,546 G→T 17.5% intergenic (+1453/‑564) USA300HOU_0902 → / → USA300HOU_0903 NADH dehydrogenase/NhaC family sodium:proton (Na+:H+) antiporter
RA 973,213 C→A 100% E175* (GAG→TAG)  USA300HOU_0930 ← possible HAD superfamily hydrolase
RA 1,002,200 T→G 100% M173R (ATG→AGG)  mecA2 → competence negative regulator MecA
RA 1,105,994 T→C 100% T12T (ACT→ACC pdhD → dihydrolipoyl dehydrogenase
RA 1,108,996 T→C 19.9% L165L (TTA→CTA)  potA → spermidine/putrescine ABC superfamily ATP binding cassette transporter, ABC protein
RA 1,162,505 A→G 16.6% I95V (ATT→GTT)  murI → glutamate racemase
RA 1,196,508 C→A 100% intergenic (+2563/‑1229) ftsQ → / → USA300HOU_1125 cell division protein FtsQ/hypothetical protein
RA 1,232,052 G→T 100% D233Y (GAT→TAT)  USA300HOU_1156 → possible PP2C protein phosphatase
RA 1,398,333 G→A 100% Y101Y (TAC→TAT msrA1 ← peptide‑methionine‑(S)‑S‑oxide reductase
RA 1,416,458 A→T 100% I216N (ATT→AAT)  USA300HOU_1314 ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
RA 1,471,910 C→T 100% E10038E (GAG→GAA ebh ← extracellular matrix binding protein
RA 1,486,381 G→T 100% Q5215K (CAA→AAA)  ebh ← extracellular matrix binding protein
RA 1,513,930 A→T 100% L413F (TTA→TTT USA300HOU_1379 → hypothetical membrane protein
RA 1,539,250 C→G 100% intergenic (‑349/+37) aroC ← / ← USA300HOU_1405 chorismate synthase/hypothetical protein
RA 1,569,145 T→C 16.0% M447V (ATG→GTG)  USA300HOU_1439 ← hypothetical bacteriophage protein
RA 1,646,189 G→C 100% A155G (GCA→GGA)  USA300HOU_1531 ← M24B family Xaa‑Pro dipeptidase
RA 1,672,590 G→T 100% L135I (CTT→ATT)  rpoD ← DNA‑directed RNA polymerase sigma subunit RpoD
RA 1,744,473 C→T 100% A67T (GCT→ACT)  apt ← adenine phosphoribosyltransferase
RA 2,010,363 C→T 100% C146Y (TGT→TAT)  USA300HOU_1861 ← ABC superfamily ATP binding cassette transporter, membrane protein
RA 2,279,705 G→A 100% intergenic (+1373/+6659) mtlD → / ← glmM mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase
RA 2,289,324 G→T 100% Q56K (CAG→AAG)  USA300HOU_2153 ← hypothetical membrane protein
RA 2,337,959 C→T 100% intergenic (‑125/‑119) USA300HOU_2195 ← / → USA300HOU_2196 possible MerR family transcriptional regulator/hypothetical protein
RA 2,455,692 Δ1 bp 100% coding (1210/1260 nt) USA300HOU_2315 → hypothetical membrane protein
RA 2,455,698 2 bp→AT 100% coding (1216‑1217/1260 nt) USA300HOU_2315 → hypothetical membrane protein
RA 2,532,298 A→T 100% T15T (ACA→ACT USA300HOU_2392 → FemAB family peptidoglycan biosynthesis protein
RA 2,679,263 A→T 26.0% intergenic (+135/‑254) USA300HOU_2535 → / → USA300HOU_2536 hypothetical protein/possible secretory antigen

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 512620 =25 (1.090)19 (1.200)
+40 bp
18/180 NT 52.4% intergenic (+688/‑173) recR/rrs1 recombination protein RecR/16S ribosomal RNA
?CP000730 1998278 = NA (NA)intergenic (‑6/+5) USA300HOU_t0050/rrf4 tRNA‑Val/5S ribosomal RNA
* ? CP000730 782414 =20 (0.870)4 (0.190) 4/240 NT 18.4% intergenic (‑209/+133) USA300HOU_0730/USA300HOU_0731 hypothetical membrane protein/hypothetical lipoprotein
?CP000730 782453 = 17 (0.800)intergenic (‑248/+94) USA300HOU_0730/USA300HOU_0731 hypothetical membrane protein/hypothetical lipoprotein