Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F191 I0 R1	49	68.0	1456600	97.2%	1415815	140.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,856,087	T→A	100%	P80P (CCT→CCA)	USA300HOU_2698 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,856,087	0	T	A	100.0%	258.1 / ‑6.5	85	P80P (CCT→CCA)	USA300HOU_2698	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (47/37); minor base C (1/0); total (48/37)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GATGCATGGCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCTAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCAAATATTAA  >  CP000730/2855952‑2856220

gaTGCATGGCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAAc                                                                                                                                      <  1:689742/140‑1 (MQ=255)

 aTGCATGGCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACg                                                                                                                                     >  1:476881/1‑140 (MQ=255)

    cATGGCGGTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTCATAGAAGATGTGTCATGCAATAATGAGCCAAAAAGtta                                                                                                                                  >  2:392397/1‑138 (MQ=255)

     aTGGCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTcaa                                                                                                                                 <  2:531486/140‑1 (MQ=255)

      tGGCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCGGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGAAGAGGTGTCATGCAATCATGCGCCAAAACGTcaac                                                                                                                                >  1:421873/1‑140 (MQ=255)

       ggCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTcaaca                                                                                                                               >  2:718745/1‑140 (MQ=255)

          gTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAAt                                                                                                                            <  1:683648/140‑1 (MQ=255)

          gTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAAt                                                                                                                            <  1:614517/140‑1 (MQ=255)

            tGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGa                                                                                                                          <  2:279687/140‑1 (MQ=255)

             ggTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGAt                                                                                                                         >  1:394535/1‑140 (MQ=255)

                ccTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAGAACGTCAACAAATGATAGa                                                                                                                      >  1:182802/1‑140 (MQ=255)

                   aaaGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAGCAAATGATAGAttt                                                                                                                   >  2:427248/1‑140 (MQ=255)

                     aggcatatCAATATTGTAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAGGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTta                                                                                                                 <  2:487180/136‑1 (MQ=255)

                       cTATAACAATATTGGAACAATCTTTATTTCATCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTtata                                                                                                               <  1:227102/140‑1 (MQ=255)

                        tataTCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAg                                                                                                              <  2:379501/140‑1 (MQ=255)

                              aaTATTGGAACAAGCTTTATTTAAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTa                                                                                                        >  2:530248/1‑140 (MQ=255)

                               atatTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATACGCCAAAACGTCAACAAAAGATAGACTTTATAATTGATA‑‑‑c                                                                                                    >  1:148780/1‑140 (MQ=255)

                               atatTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTAATCATTACCTTTTAAGTTAACAGGAGGTGTGTCATGCAATCATGCGCCCAAACGCCAACAAATGATAGATTTTATAGTTGCTA‑‑‑c                                                                                                    >  2:562966/1‑140 (MQ=255)

                                tatTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑ca                                                                                                   >  1:201936/1‑140 (MQ=255)

                                 atTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑cag                                                                                                  <  2:222642/140‑1 (MQ=255)

                                    ggAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAAACATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑cagcag                                                                                               >  1:264442/1‑140 (MQ=255)

                                        catgCTTTATTTCAGCACCAACTATATTTTAAAACGGAAGTTCAGACATTAATATCTTATAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCACCAtt                                                                                           <  1:494784/137‑1 (MQ=255)

                                        cAAGCTTTATTTCATCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCAAGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTTTAGTTGTTA‑‑‑CAGCAGCAtt                                                                                           <  1:436563/140‑1 (MQ=255)

                                        cAAGCTTTATCTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCAtt                                                                                           <  1:488375/140‑1 (MQ=255)

                                           gCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTAA‑‑‑CAGCAGCATTAGa                                                                                        >  2:46284/1‑140 (MQ=255)

                                             tttATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGcta                                                                                      >  2:679418/1‑140 (MQ=255)

                                              ttATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CCGCAGCATTAGatat                                                                                     >  2:494272/1‑140 (MQ=255)

                                               tATTTCAGCACCAACTTCATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATAtt                                                                                    >  1:360860/1‑140 (MQ=255)

                                               tATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCAAAAACGTTAACAATTGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATAtt                                                                                    <  2:476881/140‑1 (MQ=255)

                                               tATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATACAATCATGCGCCAAAACGTCAACAATTGATAGATTTTCTAGTTGTTA‑‑‑CAGCAGCATTAAATAtt                                                                                    >  1:321193/1‑140 (MQ=255)

                                                      gCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAattat                                                                             >  1:125295/1‑140 (MQ=255)

                                                         ccAACTAAATTTTAAAACGGAAGTTTAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATGGGTGTGA‑‑‑CAGCAGCATTAGATATTGAATTATTAc                                                                          <  1:208111/140‑1 (MQ=255)

                                                               aCATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCAc                                                                    <  1:382637/140‑1 (MQ=255)

                                                                  ttttAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCAGCATTAGATATTGAATTATTACGTTCAc                                                                    <  2:54241/140‑1 (MQ=255)

                                                                   tttAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGt                                                                >  1:392555/1‑140 (MQ=255)

                                                                   tttAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATATAAAAGAAGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACATTCACTGGt                                                                >  1:280715/1‑140 (MQ=255)

                                                                     tAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTa                                                              >  2:186541/1‑140 (MQ=255)

                                                                       aaaCGGAAGTTCAGTCCGCATTACCTTTTAAGTTACAAGGAAATGTGTCATGCAAGCAGGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTGA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGTTTaca                                                            >  2:381272/1‑140 (MQ=255)

                                                                          cGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTacaaca                                                         >  1:565694/1‑140 (MQ=255)

                                                                           ggAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATAACGTTCACTGGTTACAACAg                                                        >  1:699253/1‑140 (MQ=255)

                                                                           ggAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTAAATTATTACGTTCACTGGTTACAACAg                                                        >  1:323839/1‑140 (MQ=255)

                                                                           ggAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATCTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTCCAAAAg                                                        >  1:276808/1‑140 (MQ=255)

                                                                              aGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAat                                                     >  1:668445/1‑140 (MQ=255)

                                                                                ttCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAattt                                                   >  1:211959/1‑140 (MQ=255)

                                                                                  cattcatttttACTTTTTAAGTTAACAGGAGATGTGTTATGCTATCATGCGCCAAAACGTCAACAAATGATAGACTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAatttat                                                 <  1:134534/131‑1 (MQ=255)

                                                                                  cAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTATAAATGAaattat                                                 >  2:346587/1‑140 (MQ=255)

                                                                                     tcatcaTTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTCTTTg                                              >  1:143080/1‑140 (MQ=255)

                                                                                      catcatTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTCAAACAGAAGTTCTTTgg                                             >  2:571712/1‑140 (MQ=255)

                                                                                         catTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGATTACAACAGAATTTATTTGGTCt                                          >  1:421024/1‑140 (MQ=255)

                                                                                           ttACCTTTTAAGTTACCAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGt                                        <  2:221784/140‑1 (MQ=255)

                                                                                           ttACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGt                                        <  2:641871/140‑1 (MQ=255)

                                                                                            tttcTTTTAAGTTAGCAGGAGATGTGGCATGCAATTATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTGCACTGGTTACAACAGAATGTATTTGGTCTGTc                                       <  2:147288/137‑1 (MQ=255)

                                                                                            tACCTTTTAAGTCAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAATAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTc                                       <  1:437898/140‑1 (MQ=255)

                                                                                              ccTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTccc                                     <  1:515385/140‑1 (MQ=255)

                                                                                               cTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCt                                    <  1:131885/140‑1 (MQ=255)

                                                                                               cTTGTAAGTTAACAGGAGATGTGTAGTGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCt                                    <  2:427924/140‑1 (MQ=255)

                                                                                                ttttAAGTTAACAGGAGATGTGTCATGCAATCATGCTCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTg                                   <  1:589956/140‑1 (MQ=255)

                                                                                                 tttAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAATAGAATTTATTTGGTCTGTCCCTgg                                  <  1:530248/140‑1 (MQ=255)

                                                                                                  ttAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGc                                 <  2:649775/140‑1 (MQ=255)

                                                                                                  ttAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGc                                 <  1:130963/140‑1 (MQ=255)

                                                                                                   tcaGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGcc                                <  1:362958/138‑1 (MQ=255)

                                                                                                    gaGTTAACAGGGGATGTGTCATGCAATCATGCTCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTGTTTGGTCTGTCCCTGGCCg                               <  2:407789/139‑1 (MQ=255)

                                                                                                       ttAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCtt                            <  1:718745/140‑1 (MQ=255)

                                                                                                       ttAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCtt                            <  1:576598/140‑1 (MQ=255)

                                                                                                         aaCAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGGTATTATAGTTTTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTg                          >  2:541/1‑140 (MQ=255)

                                                                                                         aaCAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTg                          >  2:379004/1‑140 (MQ=255)

                                                                                                         aaCAGGAGATGTGTCATGCAATCAAGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTg                          >  1:465951/1‑140 (MQ=255)

                                                                                                            aGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACa                       >  2:287046/1‑140 (MQ=255)

                                                                                                             ggAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACat                      >  2:635699/1‑140 (MQ=255)

                                                                                                             ggAGATGTGTCATGCAATCATGCGCCAAAACGGCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACat                      >  2:514562/1‑140 (MQ=255)

                                                                                                                  tgtgTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGCAAtatagg                 >  2:604168/1‑138 (MQ=255)

                                                                                                                  tgtgTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACg                 <  2:174412/140‑1 (MQ=255)

                                                                                                                  tgtgTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTAATTGATGCGTCCCTGGCCGCTTTGACATATACg                 <  1:533925/140‑1 (MQ=255)

                                                                                                                   gtgtCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGGCATATACgg                >  2:73659/1‑140 (MQ=255)

                                                                                                                   gtgtCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACgg                >  1:475086/1‑140 (MQ=255)

                                                                                                                    tttCATGCCATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTTGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGGCTGTCCCTGGCCGCTTTGACATATACGGa               <  1:392397/138‑1 (MQ=255)

                                                                                                                    tgtCATGCAATCATGCGCCAAAACGTCAACAAATGATAGAAATTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGa               >  1:319875/1‑140 (MQ=255)

                                                                                                                     gtCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGAc              >  2:259885/1‑140 (MQ=255)

                                                                                                                      tCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGAcc             >  1:701349/1‑140 (MQ=255)

                                                                                                                       cATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATCCGGACCt            >  2:208717/1‑140 (MQ=255)

                                                                                                                             aTCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCAAata      >  1:302097/1‑140 (MQ=255)

                                                                                                                             aTCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCAAata      <  1:104659/140‑1 (MQ=255)

                                                                                                                              tCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGAAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCAAatat     >  2:637102/1‑140 (MQ=255)

                                                                                                                               cATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCAAATAtt    <  1:186541/140‑1 (MQ=255)

                                                                                                                                 tGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTAACATATACGGACATCAAATATTaa  >  2:647558/1‑140 (MQ=255)

GATGCATGGCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCTAAACGTCAACAAATGATAGATTTTATAGTTGTTA‑‑‑CAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCAAATATTAA  >  CP000730/2855952‑2856220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A