Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F184 I0 R1	46	69.8	1527574	94.8%	1448140	140.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,064,254	Δ1 bp	100%	coding (235/1218 nt)	USA300HOU_1000 ←	LytR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,064,253	0	C	.	95.0%	343.2 / 7.8	78	coding (236/1218 nt)	USA300HOU_1000	LytR family transcriptional regulator
Reads supporting (aligned to +/- strand): ref base C (2/1); new base . (42/32); total (44/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGTAATCTTATCGTAATAGCCAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCCTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGACGATTAGCAT‑TTTTGCTGTCTTGAGAAGATTCGA  >  CP000730/1064114‑1064385

ggTAATCTTATCGTAATAGCCAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTc                                                                                                                                        >  1:236435/1‑140 (MQ=255)

ggTAATCTTATCGTAATAGCCAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTc                                                                                                                                        >  2:661622/1‑140 (MQ=255)

 gTAATCTTATCGTAATAGCCAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATGTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTct                                                                                                                                       <  1:252191/140‑2 (MQ=255)

  tAATCTTATCGTAATAGCCAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTctt                                                                                                                                      <  1:370442/140‑3 (MQ=255)

  tAATCTTATCGTAATAGCCAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTctt                                                                                                                                      >  1:634880/1‑138 (MQ=255)

    aTCTTATCGTAATAGCCAACTTTATGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCtttt                                                                                                                                    <  1:422511/140‑1 (MQ=255)

            gTAATAGCCAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTt‑tcttct                                                                                                                          <  2:610474/140‑1 (MQ=255)

               aTAGTCAACTTTAGGAATGTAGCTTATAGTATCACGTGGAATACTAAGCATTCTAATTTTATTTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTcc                                                                                                                       <  1:89759/140‑1 (MQ=255)

                 aGCCAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTGAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTAAGCATCCTGT‑CTTTTTT‑TTTTCTTCCAt                                                                                                                     >  2:720415/1‑140 (MQ=255)

                   ccAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTATGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTTTTCCTTCg                                                                                                                   >  2:272310/1‑140 (MQ=255)

                    cAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATAATAAGCCTTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTATAATCAAAGCGGCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGt                                                                                                                  <  1:440553/140‑1 (MQ=255)

                     aaCTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGtt                                                                                                                 >  1:740977/1‑140 (MQ=255)

                      aCTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAAAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTa                                                                                                                >  2:686598/1‑140 (MQ=255)

                        tttAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTCACTTAGAGTGTTCAGAATACTGT‑ATTTTTT‑TCTTCTTccgttcttttc                                                                                                              >  1:476066/1‑131 (MQ=255)

                         ttAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTatca                                                                                                             <  2:598845/140‑1 (MQ=255)

                          tAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTatcat                                                                                                            >  1:83126/1‑140 (MQ=255)

                            ggAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCg                                                                                                          <  2:475525/140‑1 (MQ=255)

                            ggAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTCTT‑TCTTCTTCCATCGTTATCATCg                                                                                                          <  1:486027/140‑1 (MQ=255)

                            ggAATGTAGCTGATAGTATCACGTGGAATACTAAGCATACTACTTTGATGTTTTGATTGATTAAAAGTAGTTAAAATAGTAGCGTATGATATCGAGAGTTAAGAATACTGT‑CTTTTTT‑TCTTGTTCCATCGTTATCATCg                                                                                                          >  1:463503/1‑140 (MQ=255)

                                 gTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAACAGTAGTTAAAATAATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCTTCGTTATCTTCGATaac                                                                                                     >  2:270181/1‑138 (MQ=255)

                                 gTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TTTTTTTTTATTGTTATCATCGATccc                                                                                                     >  2:298208/1‑140 (MQ=255)

                                    gCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTaa                                                                                                  <  2:710473/140‑1 (MQ=255)

                                      tGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAGAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TATTCTTCCATCGTTATTAGAGATACCTaaga                                                                                                >  1:99060/1‑140 (MQ=255)

                                       gATAGTATCATGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTaagaa                                                                                               <  1:564378/140‑1 (MQ=255)

                                       gATAGTATCAAGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTaagaa                                                                                               <  1:356243/140‑1 (MQ=255)

                                        aTAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAAAGAGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTaataaa                                                                                              >  1:5134/1‑140 (MQ=255)

                                          aGTATCACGTGGAATACTAAGCATGCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTaagaaaag                                                                                            <  2:740162/140‑1 (MQ=255)

                                            tATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTaagaaaagaa                                                                                          >  1:443784/1‑140 (MQ=255)

                                              tCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATa                                                                                        >  2:708313/1‑140 (MQ=255)

                                                 cGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATagag                                                                                     >  2:474484/1‑140 (MQ=255)

                                                  gTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAgaga                                                                                    <  1:122907/140‑1 (MQ=255)

                                                    ggAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATa                                                                                  >  1:106762/1‑140 (MQ=255)

                                                     gAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTCTCATCGATACCTAAGAAAAGAATAGAGATAg                                                                                 >  2:702735/1‑140 (MQ=255)

                                                       aTACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGgt                                                                               >  2:109223/1‑140 (MQ=255)

                                                       aTACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGgt                                                                               >  2:292309/1‑140 (MQ=255)

                                                        tACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGGGGGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGgtt                                                                              <  1:672066/140‑1 (MQ=255)

                                                        tACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGgtt                                                                              <  1:572123/140‑1 (MQ=255)

                                                        tACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGgtt                                                                              <  1:572113/140‑1 (MQ=255)

                                                        tACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTATTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGgtt                                                                              <  1:718083/140‑1 (MQ=255)

                                                          cTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCCTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTACGAACAGAATATAGATAGgttg                                                                             >  2:686701/1‑140 (MQ=255)

                                                            aaGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGttc                                                                          >  1:508517/1‑140 (MQ=255)

                                                            aaGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGAGTCAGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTAATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGttc                                                                          >  1:632460/1‑140 (MQ=255)

                                                                  tCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTGTTCCATCGTTATCATCGATACCAAAGAAAAGAATAGAGATAGGTTGTTCTTCggg                                                                    >  1:359946/1‑140 (MQ=255)

                                                                  tCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCggg                                                                    >  1:22599/1‑140 (MQ=255)

                                                                   cTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGa                                                                   <  2:296485/140‑1 (MQ=255)

                                                                   cTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAAAGATAGgtttttgcgcttaa                                                                   >  2:530271/1‑132 (MQ=255)

                                                                       tttGATTCTTTAATTGATTAAAGGTATTGAAAATCATAGCGTCTGATCTAGAGTGTTAATTATCCT‑TTCTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGa                                                               <  1:203360/140‑1 (MQ=255)

                                                                       tttGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAAAAAAGAATAGAGATAGGTTGTTCTTCGGGATTgc                                                               >  2:194301/1‑139 (MQ=255)

                                                                        ttGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTCCAGCGTTCAGCATCCTGTCTTTTTTTAACCTAATACTTCG‑‑ACCAGCCATACCTTAGAAAAGAATAGAGATAGGTTGTTCGTCGGGATTGAc                                                              >  2:730790/1‑140 (MQ=255)

                                                                        ttGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTGCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGAc                                                              >  2:544389/1‑140 (MQ=255)

                                                                         tGATGTTTTGATTGTTTAAAAGTAGTTAATATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACt                                                             <  1:116513/140‑1 (MQ=255)

                                                                         tGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACt                                                             <  1:570671/140‑1 (MQ=255)

                                                                           aTGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACttt                                                           >  1:29772/1‑140 (MQ=255)

                                                                            tGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTtta                                                          <  1:204593/140‑1 (MQ=255)

                                                                               tttGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTttatta                                                       <  1:756893/140‑1 (MQ=255)

                                                                                 tgattgatTAAAAGTAGTTAAAATCATAGCGGCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTAtc                                                     <  2:329459/140‑1 (MQ=255)

                                                                                        ttAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAg                                              <  2:490360/140‑1 (MQ=255)

                                                                                        ctAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAg                                              <  2:644522/139‑1 (MQ=255)

                                                                                             agtagtTAAAATCAGAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAAAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGa                                         >  1:397199/1‑140 (MQ=255)

                                                                                              gtagtTAATATCATAGCGTCTGATCTAGAGTGTTCCTCATCCTGT‑TTTTTTT‑TTTTCTTCCATCGTTATCATGGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGAt                                        <  1:236001/140‑1 (MQ=255)

                                                                                              gtagtTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGAt                                        <  1:696667/140‑1 (MQ=255)

                                                                                                 gtTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGa                                     >  1:657638/1‑140 (MQ=255)

                                                                                                  ttAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGAc                                    <  2:740977/140‑1 (MQ=255)

                                                                                                     aaaTCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TTTTTTTTTTTCGTTATCATCGATACCTAAGAAAAGAATAGTGATAGGTTGTTCTTCGGGATTGACTTTATTATCTATTAAGTTGGATAGACGAt                                 >  2:76297/1‑140 (MQ=255)

                                                                                                     aaaTCATAGCGTCTGATCTAGAGTGTTCAGCATACTGT‑CTTTTTT‑TTTTCTTTATTTATTATCAACAGTGCATAAGCAAAGAATAGAAATGGTTATTTCTTTGGATTTGACTTTTTTATCGATTAAGTTTGATAGACTAt                                 >  2:230013/1‑140 (MQ=255)

                                                                                                     aaaTCATACCGTCTGAACTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCGATCGTAATCATCTATACCTAAGAAAAGAATAATGATATGTTGTTGTTCTGGATTGACTTTATTATCTCTTCAGTTGGAttacagat                                 >  2:214983/1‑134 (MQ=255)

                                                                                                      aaTCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGACGAtt                                <  1:474484/140‑1 (MQ=255)

                                                                                                      aaTCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGACGAtt                                >  1:604453/1‑140 (MQ=255)

                                                                                                       aTCATAGCGTCTGATCTAGATTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGACGATTa                               <  2:179090/140‑1 (MQ=255)

                                                                                                        tCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTG‑TCTTCTTCCATCGTTATCATCGATACCCAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTACGTTGGATTGACGATTAg                              >  1:519914/1‑140 (MQ=255)

                                                                                                        ccATAGCGTCTGATCTAGCGTGTTCAGCCTCCTGT‑GTTTTTT‑TCTTCTTCCAGCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGACGATTAg                              <  2:135526/139‑1 (MQ=255)

                                                                                                          aTAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TTTTTTTTTATCGTTATAATCGGTGCCTAAGAAAAGAATAGAGATAGGTGGTTCTTCGGGGTTGACTTTCTTATCTCTTAAAAAGGATTGCCGAGTAGCa                            >  2:674194/1‑140 (MQ=255)

                                                                                                           tAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATTTTCGTTACTTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGACGATTAGCAt                           <  1:250173/140‑1 (MQ=255)

                                                                                                           tAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGACGATTAGCAt                           >  1:645444/1‑140 (MQ=255)

                                                                                                           tAGCGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGACGATTAGCAt                           >  1:447026/1‑140 (MQ=255)

                                                                                                              cGTCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGACGATTAGCAt‑ttt                       <  1:708313/140‑1 (MQ=255)

                                                                                                                tCTGATCTAGAGTGTTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGACGATTAGCAT‑TTTTg                     <  1:429597/140‑1 (MQ=255)

                                                                                                                           gtgtTCAGCATCCTGT‑CTTTTTT‑TTTTTTTTTTTTTTTATCATCGATACCTAAGAAAAGAATAGAGTTAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGACGATTAGCAT‑TTTTTCTTTCTTtagt          >  2:289047/1‑139 (MQ=255)

                                                                                                                             gtTCAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGACGATTAGCAT‑TTTTGCTGTCTTGagaag        >  1:627132/1‑140 (MQ=255)

                                                                                                                                cAGCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATAATCTCTTAAGTTGGATTGTCGCATA‑CATCTTTTTCTGTCTTgcacaaatt     >  1:511116/1‑132 (MQ=255)

                                                                                                                                  gCATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTTAGCAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGTATTGTCGATTAGCAT‑TTTTGCTTTCTTGTGAATATTCg   >  2:405094/1‑140 (MQ=255)

                                                                                                                                   cATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGACGATTAGCAT‑TTTTGCTGTCTTGAGAAGATTCGa  >  1:641098/1‑140 (MQ=255)

                                                                                                                                   cATCCTGT‑CTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGACGATTAGCAT‑TTTTGCTGTCTTGAGAAGATTCGa  >  1:539605/1‑140 (MQ=255)

GGTAATCTTATCGTAATAGCCAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCCTTTTTT‑TCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGACGATTAGCAT‑TTTTGCTGTCTTGAGAAGATTCGA  >  CP000730/1064114‑1064385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A