breseq  version 0.32.1  revision 5f2cb669173e
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 3,790 G→A 100% A32T (GCA→ACA)  USA300HOU_0003 → hypothetical protein
RA 39,378 C→A 100% D586Y (GAT→TAT)  mecA ← penicillin binding protein 2 prime
RA 76,969 G→A 100% intergenic (+1027/‑107) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 110,651 C→T 10.5% D170D (GAC→GAT USA300HOU_0109 → staphylococcal tandem lipoprotein
RA 110,660 T→A 13.1% I173I (ATT→ATA USA300HOU_0109 → staphylococcal tandem lipoprotein
RA 111,512 A→G 33.9% S194S (TCA→TCG USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 111,516 C→A 34.5% Q196K (CAA→AAA)  USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 195,286 T→A 100% intergenic (‑97/‑82) USA300HOU_0183 ← / → USA300HOU_0184 hypothetical protein/hypothetical protein
RA 355,466 G→A 100% P412S (CCA→TCA)  brnQ1 ← LIVCS family branched chain amino acid:cation symporter
RA 396,540 G→A 100% A208T (GCA→ACA)  USA300HOU_0364 → possible lipoprotein
RA 512,643 A→G 43.0% intergenic (+711/‑150) recR → / → rrs1 recombination protein RecR/16S ribosomal RNA
RA 607,884 G→A 100% R209* (CGA→TGA)  USA300HOU_0550 ← deoxynucleoside kinase
RA 618,567 T→C 10.3% D1223D (GAT→GAC sdrD → Ser‑Asp rich fibrinogen/bone sialoprotein‑binding protein SdrD
RA 629,681 C→A 100% L84I (CTA→ATA)  USA300HOU_0564 → possible 3‑hexulose‑6‑phosphate synthase
RA 665,392 T→G 100% L79* (TTA→TGA)  USA300HOU_0608 → possible acetytransferase
RA 707,910 A→G 100% intergenic (‑1/‑121) USA300HOU_0653 ← / → USA300HOU_0654 ABC superfamily ATP binding cassette transporter, ABC protein/possible metal‑dependent transcriptional regulator
RA 874,718 A→C 100% K541N (AAA→AAC clfA → fibrinogen‑binding protein
RA 1,056,257 C→A 17.1% intergenic (‑1758/+1909) sspA ← / ← USA300HOU_0997 S01 family glutamyl endopeptidase/bifunctional N‑acetylmuramoyl‑L‑alanine amidase/mannosyl‑glycoprotein endo‑beta‑N‑acetylglucosaminidase
RA 1,064,254 Δ1 bp 100% coding (235/1218 nt) USA300HOU_1000 ← LytR family transcriptional regulator
RA 1,217,669 T→A 10.1% *71K (TAA→AAA)  USA300HOU_1144 → hypothetical protein
RA 1,233,504 T→A 100% V470D (GTT→GAT)  USA300HOU_1157 → non‑specific serine/threonine protein kinase
RA 1,416,458 A→T 100% I216N (ATT→AAT)  USA300HOU_1314 ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
RA 1,440,085 G→T 100% intergenic (‑45/‑891) cspA ← / → xpaC cold shock protein CspA/5‑bromo‑4‑chloroindolyl phosphate hydrolysis protein XpaC
RA 1,513,930 A→T 100% L413F (TTA→TTT USA300HOU_1379 → hypothetical membrane protein
RA 1,646,189 G→C 100% A155G (GCA→GGA)  USA300HOU_1531 ← M24B family Xaa‑Pro dipeptidase
RA 1,662,436 G→A 9.6% P657L (CCT→CTT)  pbp3 ← penicillin‑binding protein 3
RA 1,744,362 G→A 100% H104Y (CAC→TAC)  apt ← adenine phosphoribosyltransferase
JC 1,747,382 (TAC)3→2 95.3% coding (2065‑2067/2280 nt) secDF ← RND superfamily resistance‑nodulation‑cell division protein:proton (H+) antiporter
RA 1,761,889 C→A 100% D388Y (GAT→TAT)  folC ← tetrahydrofolate synthase
RA 1,963,768 C→G 100% V365L (GTA→CTA)  hemG ← protoporphyrinogen oxidase
RA 2,010,363 C→T 100% C146Y (TGT→TAT)  USA300HOU_1861 ← ABC superfamily ATP binding cassette transporter, membrane protein
RA 2,027,672 C→A 100% G92V (GGC→GTC)  vraS ← sensor histidine kinase VraS
RA 2,241,539 T→C 60.8% intergenic (‑182/+284) murZ ← / ← fbaA UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase/fructose‑bisphosphate aldolase
RA 2,269,344 A→G 6.0% V55A (GTA→GCA)  USA300HOU_2143 ← possible HAD superfamily hydrolase
RA 2,279,705 G→A 100% intergenic (+1373/+6659) mtlD → / ← glmM mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase
RA 2,289,262 C→G 100% W76C (TGG→TGC USA300HOU_2153 ← hypothetical membrane protein
RA 2,352,815 G→A 100% A92V (GCA→GTA)  rpsI ← ribosomal protein S9
RA 2,409,590 A→G 5.9% Q127R (CAA→CGA)  ureG → urease accessory protein UreG
RA 2,454,626 A→T 100% L48L (CTA→CTT USA300HOU_2315 → hypothetical membrane protein
JC 2,501,544 Δ108 bp 9.8% coding (68‑175/2106 nt) USA300HOU_2364 → AraC transcriptional regulator
RA 2,532,298 A→T 100% T15T (ACA→ACT USA300HOU_2392 → FemAB family peptidoglycan biosynthesis protein
RA 2,544,276 T→C 8.5% Y168Y (TAT→TAC hlgC → gamma hemolysin component C
RA 2,544,339 C→T 100% V189V (GTC→GTT hlgC → gamma hemolysin component C
RA 2,614,662 A→G 7.8% I3I (ATT→ATC USA300HOU_2478 ← lipoprotein
RA 2,709,576 C→T 93.7% G451S (GGT→AGT)  USA300HOU_2562 ← possible acetyltransferase
RA 2,712,590 A→G 3.9% S105P (TCA→CCA)  isaA ← immunodominant antigen A

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 512620 =92 (1.430)50 (1.200)
+45 bp
35/168 NT 49.4% intergenic (+688/‑173) recR/rrs1 recombination protein RecR/16S ribosomal RNA
?CP000730 2176447 = 66 (1.020)noncoding (114/114 nt) rrf5 5S ribosomal RNA