Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F174 I0 R1 55 81.0 1740380 95.7% 1665543 141.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,963,768 C→G 100% V365L (GTA→CTA)  hemG ← protoporphyrinogen oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,963,7680CG100.0% 259.6 / ‑6.5 85V365L (GTA→CTA) hemGprotoporphyrinogen oxidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (37/46);  minor base T (1/0);  total (38/47)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTTTTCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATG  >  CP000730/1963632‑1963901
                                                                                                                                        |                                                                                                                                     
cTTTTCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATAc                                                                                                                                   >  1:258747/1‑141 (MQ=255)
    tCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTTATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCAc                                                                                                                               <  2:293388/141‑1 (MQ=255)
     cGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACg                                                                                                                              >  2:689174/1‑141 (MQ=255)
        ctaaCGATTGATAATTGTAACGTCAGGATCACCTTTAAGCGTCGTCATTTGACTTAAATCTCTACTTACAATCGATACTCATTCCTTATCTGTATGATCATCAACCCCAGTATCACCTGGTTTACCTAGGTACGCACGagt                                                                                                                           <  2:700675/139‑3 (MQ=255)
        cAAACGATTGACAATTGTAAATTCAGTATCACCTTTAAATGTCATCATTTGACTTAAATCTCTTCATACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAAt                                                                                                                           <  1:200187/141‑1 (MQ=255)
        cAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGCCTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCCCGAAt                                                                                                                           >  2:312460/1‑141 (MQ=255)
        cAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAAt                                                                                                                           >  2:249487/1‑141 (MQ=255)
         aaaCGATTGACAATTGTAAATTCAGGACCACCTTTAAATGTCATCATTTGACTTAAATCTCTACTTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATc                                                                                                                          <  2:315537/141‑1 (MQ=255)
         aaaCGAGTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGCACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACTAACCAATc                                                                                                                          <  2:315536/141‑1 (MQ=255)
          aaCGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTCGATACGCCCGAATCa                                                                                                                         >  2:277876/1‑141 (MQ=255)
               ttGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTATATAGCCTCGAATCAAAaca                                                                                                                    >  2:826233/1‑140 (MQ=255)
               ttGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAacc                                                                                                                    >  2:849910/1‑141 (MQ=255)
               ttGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAAGCAAAacc                                                                                                                    >  2:659142/1‑141 (MQ=255)
                tGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAacct                                                                                                                   >  1:762233/1‑141 (MQ=255)
                       tGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTc                                                                                                            <  2:827246/141‑1 (MQ=255)
                          aaaTTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCgg                                                                                                         >  1:343838/1‑141 (MQ=255)
                           aaTTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACACTCGACACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCgcg                                                                                                        >  1:240385/1‑139 (MQ=255)
                            aTTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGgtg                                                                                                       <  1:785221/141‑1 (MQ=255)
                            aTTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGgtg                                                                                                       <  2:412614/141‑1 (MQ=255)
                             ttCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGgtgt                                                                                                      <  1:234055/141‑1 (MQ=255)
                             ttCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGAATCGCAGGAATCAAAACCTTACCTTCCGgtgt                                                                                                      <  1:205900/141‑1 (MQ=255)
                                 ggATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTgtagta                                                                                                  >  2:54746/1‑141 (MQ=255)
                                      cccTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATgg                                                                                             <  1:644538/140‑1 (MQ=255)
                                       ccTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGc                                                                                            <  1:293827/141‑1 (MQ=255)
                                        ttttAAATTTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTTTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGcc                                                                                           <  2:144468/140‑1 (MQ=255)
                                          ttAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCAt                                                                                         >  1:37122/1‑141 (MQ=255)
                                            aaaTGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGTCCAttt                                                                                       <  1:710014/141‑1 (MQ=255)
                                              aTGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCAttttt                                                                                     <  1:693126/141‑1 (MQ=255)
                                                gTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGAACGAATCAAAACCTTACCTTCCGGCGTAGTAAATGGCCAttttttg                                                                                   >  2:10376/1‑140 (MQ=255)
                                                 tcatcaTTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCg                                                                                  <  2:304432/141‑1 (MQ=255)
                                                         tGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCaa                                                                          <  1:400208/141‑1 (MQ=255)
                                                         tGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCaa                                                                          <  1:272495/141‑1 (MQ=255)
                                                         tGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCaa                                                                          >  2:833973/1‑141 (MQ=255)
                                                          gACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAg                                                                         <  2:484628/141‑1 (MQ=255)
                                                          gACTTAAATCTCTACGTACAATCGAGACTAATTCATTATCTGTATGATCATCACCCACAGTATCACCTGGTTTACCCAGATATGACCGAAACCAAAACTTACCTTCCGGTTTAGTAAATGTCCAGGTTTTTTATGTCCaat                                                                         <  2:724782/141‑2 (MQ=255)
                                                              tAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACa                                                                     <  2:106149/141‑1 (MQ=255)
                                                                   ccctACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTACCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCgg                                                                <  1:28998/139‑1 (MQ=255)
                                                                    tctACGTACAAACGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATTAAAACCTTACCTTCCGGTGTAGTAAATGGCCATGTTTTCGATGTCCAAGTACATGCGGt                                                               <  2:480861/141‑1 (MQ=255)
                                                                      tACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGAATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTaa                                                             >  2:745621/1‑141 (MQ=255)
                                                                         gTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACTTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGt                                                          <  2:742755/141‑1 (MQ=255)
                                                                           aCAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCt                                                        >  2:365248/1‑141 (MQ=255)
                                                                              aTCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTa                                                     >  2:412888/1‑141 (MQ=255)
                                                                                cGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATc                                                   <  2:9400/141‑1 (MQ=255)
                                                                                cGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATc                                                   <  1:404744/141‑1 (MQ=255)
                                                                                cGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATc                                                   <  2:132714/141‑1 (MQ=255)
                                                                                  ataataATTCATTATCTGTATGATAATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAACCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTGTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCAc                                                 <  2:757952/141‑1 (MQ=255)
                                                                                   tACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGTTGTCCAAGTACATGAGGTAATGTCTGTTTCACt                                                >  2:740435/1‑141 (MQ=255)
                                                                                   tACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACt                                                >  2:633904/1‑141 (MQ=255)
                                                                                    aCTAATTCAGTATCTGTATGATCCTCACCCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTc                                               <  2:343838/141‑1 (MQ=255)
                                                                                     cTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCCCGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAACTACCTGCCGCAATGTCAGTATCACTCg                                              >  2:469132/1‑141 (MQ=255)
                                                                                        aTTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTtc                                           >  2:704111/1‑141 (MQ=255)
                                                                                          tCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATCCGGTACTGTCTGTATCACTCGTtctc                                         >  2:47695/1‑141 (MQ=255)
                                                                                          tCATTATCTGTACGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTtctc                                         <  1:818227/141‑1 (MQ=255)
                                                                                            aTTATCTTTCTGTTCATCAACCACATTATCACCTGGTTTTCCTAGATACGCACGCATCAAAACCTTACCTTCCTGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCTCTCGTTCTCGc                                       <  2:297130/141‑1 (MQ=255)
                                                                                                tCTGTATGATCATCAACCACAGTATCTCCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAAtt                                   <  1:783273/141‑1 (MQ=255)
                                                                                                tCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTTTCGCAAtt                                   >  2:668812/1‑141 (MQ=255)
                                                                                                tCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAAtt                                   >  2:668831/1‑141 (MQ=255)
                                                                                                  tttATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTAc                                 <  2:495638/139‑1 (MQ=255)
                                                                                                        atcatcAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGcc                           <  2:445931/141‑1 (MQ=255)
                                                                                                           atccACCACAGTATCACCTGGTTTACCTAGATTCGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGt                        <  2:578161/141‑1 (MQ=255)
                                                                                                           atcaACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATCTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTTTCGTAATTACGAAGCCAGt                        <  1:646653/141‑1 (MQ=255)
                                                                                                           accaACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGCCTGTATCACTCGTTCTCGCAATTACGAAGCCAGt                        <  2:575179/139‑1 (MQ=255)
                                                                                                             caACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTAc                      <  2:719488/141‑1 (MQ=255)
                                                                                                              aaCCACAGTATCACCTGGTTTACCTCGATACGCACGAATCAAAACCTTCCCTTCCGGTGTAGTAAATGGCCCTTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTAcc                     <  1:354699/141‑1 (MQ=255)
                                                                                                               aCCACAGTATCACCTGGTGTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGGCTGTATCACTCGTTCTCGCAGTTACGAAGCCAGTACca                    >  2:488663/1‑141 (MQ=255)
                                                                                                                ccACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGTGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACcat                   >  2:444071/1‑141 (MQ=255)
                                                                                                                   ccggaTCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACcatcat                <  2:509242/137‑1 (MQ=255)
                                                                                                                   caGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTTTATTTCTAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGATGCCAGTACcatcat                >  2:504276/1‑141 (MQ=255)
                                                                                                                   caGTATCACCTGGTTTACCTAGACACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCCTTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACcatcat                <  2:509286/141‑1 (MQ=255)
                                                                                                                    aGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATg               >  1:689754/1‑141 (MQ=255)
                                                                                                                     gTATCACCTGGTTTACCTAGATACGCCCGAATCAACACCTTACCTTCCGGTGAAGTACATGACAATTTTTTCGATGTCCACGACCATACGGACAGGTATGTAACACTCGGTCTCGCAATTGCGAAGCCGGCACCATCAggg              >  2:256161/1‑141 (MQ=255)
                                                                                                                      tATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATggg             <  1:434361/141‑1 (MQ=255)
                                                                                                                        tCACCTGGTTTACCTAGATATGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGtt           >  1:666651/1‑140 (MQ=255)
                                                                                                                        tCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTa           >  1:617233/1‑141 (MQ=255)
                                                                                                                          aCCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTTTTTTTTTTTTTACATTCGGTAATGTCTGTGTTACTCGTTTTCGCAATTACGAAGCCAGTAGGATCATGGGTAtt         >  2:164822/1‑141 (MQ=255)
                                                                                                                          aCCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTCtt         >  1:208190/1‑141 (MQ=255)
                                                                                                                          aCCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTAtt         >  1:207829/1‑141 (MQ=255)
                                                                                                                          aCCTAGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTAtt         >  2:529680/1‑141 (MQ=255)
                                                                                                                           ccTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAGTGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTAttt        >  2:49895/1‑141 (MQ=255)
                                                                                                                             tGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTc      <  2:258747/141‑1 (MQ=255)
                                                                                                                              ggTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACACGTTCTCGCAATTACGAAGCCAGTACCACCATGGGTATTTTCa     >  2:415600/1‑141 (MQ=255)
                                                                                                                              ggTTTACCTAGATACGCACGAATCAAAACCCTCCCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCa     <  1:220885/141‑1 (MQ=255)
                                                                                                                               ttttACCTAAATACGAACGCATGAAAACTTTACCTTCCGTTGTAGTAAATGGCCACTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCaa    <  2:845864/140‑1 (MQ=255)
                                                                                                                                 ttACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATg  <  1:108136/141‑1 (MQ=255)
                                                                                                                                 ttACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGACACTATTTGCAGTTGTATTTTCAATg  <  2:837900/141‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                     
CTTTTCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATG  >  CP000730/1963632‑1963901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A