Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F123 I0 R1 46 52.8 1143652 95.1% 1087613 141.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,963,768 C→G 100% V365L (GTA→CTA)  hemG ← protoporphyrinogen oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,963,7680CG100.0% 223.6 / ‑6.5 66V365L (GTA→CTA) hemGprotoporphyrinogen oxidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (34/30);  minor base T (1/1);  total (35/31)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATG  >  CP000730/1963635‑1963901
                                                                                                                                     |                                                                                                                                      
ttCGGCAAACTATCGACAATTGAAAAGTCAGTATCACCTTTAAATGTCATCATTTTACTTAAATCTCTACGTACAATCGATACTAATTCAGTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCa                                                                                                                                 <  2:356556/141‑1 (MQ=255)
       aaCGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCCCG‑AATCa                                                                                                                         >  1:330443/1‑141 (MQ=255)
               aCAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑ACTCAAAaccttc                                                                                                                 >  2:551936/1‑140 (MQ=255)
               aCAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAacctta                                                                                                                 >  2:553737/1‑141 (MQ=255)
               aCAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAacctta                                                                                                                 >  1:471892/1‑141 (MQ=255)
                  aTTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAaccttacct                                                                                                              <  2:456628/141‑1 (MQ=255)
                    tGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTc                                                                                                            >  2:323023/1‑141 (MQ=255)
                        aaTTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCggg                                                                                                        >  2:437075/1‑140 (MQ=255)
                         aTTCAGGCTCACCTTTAAGTGTCATCATTTGACTTAACTCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGgtg                                                                                                       <  1:464887/141‑1 (MQ=255)
                          ttCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACGATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGgtgt                                                                                                      <  1:361225/141‑1 (MQ=255)
                          ttCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAATCCTTACCTTCCGgtgt                                                                                                      <  2:551133/141‑1 (MQ=255)
                          ttCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATAACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGgtgt                                                                                                      >  1:267371/1‑141 (MQ=255)
                          ttCAGGATCACCTCTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGgtgt                                                                                                      <  2:427414/141‑1 (MQ=255)
                            cAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTgtag                                                                                                    >  2:182767/1‑141 (MQ=255)
                            cAGGACCACCTTTAAATTTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTgtag                                                                                                    <  2:192120/141‑1 (MQ=255)
                              ggATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTGATTCATTCTCTGTATGATCATCAAACACAGTATGACCTCGTGTACATAGATACG‑ACGTAATCAAAACCTTACCGTACggagcaaga                                                                                                  >  2:268232/1‑134 (MQ=255)
                                  cACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATg                                                                                              >  2:436128/1‑141 (MQ=255)
                                   cccTTTAAACGTAATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATgg                                                                                             <  2:38411/140‑1 (MQ=255)
                                    ccTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGc                                                                                            <  2:372784/141‑1 (MQ=255)
                                    ccTTTAAATGTCATCATTTGACTTAAATCTCCACGTACAAGCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGc                                                                                            <  2:507571/141‑1 (MQ=255)
                                     cTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGCTACTCATAAATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTTCCTATATACGCACG‑AATCAAAACCGTACCTTCCGGGGTAGTAAATGGcc                                                                                           >  1:474461/1‑141 (MQ=255)
                                       ttAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCAt                                                                                         >  2:338635/1‑141 (MQ=255)
                                         aaaTGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCAttt                                                                                       <  1:553543/141‑1 (MQ=255)
                                             ttcatcaTTTGACTTAAATCTTTACGTACAATCGATACTAATTCATTATCTGTATGATCCTCAACCACATTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCATACCGTCCGGTGTAGTAAAGGGCCATTTTTTc                                                                                   <  2:238133/140‑1 (MQ=255)
                                             gTCATCGTGTGACTGAAATCTCTACGTACAACCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTc                                                                                   <  1:411924/141‑1 (MQ=255)
                                             gTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCAttttttt                                                                                   >  2:519989/1‑140 (MQ=255)
                                              tcatcaGTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCg                                                                                  <  1:3373/141‑1 (MQ=255)
                                               catcatTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGa                                                                                 >  1:500030/1‑141 (MQ=255)
                                                 tcatTTGACTTAAAACTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATg                                                                               <  1:437075/141‑1 (MQ=255)
                                                 ccatTTGACTTAAATCTCTGCCCCCAATGCATACTAATTCATTACGTGCATGACCATCAACCACAGTATCACCTGGTTTACCTATATACGCACG‑AATCAAACCCTTACCTTCCGGTGTAGTAAACGGCCTTTTTTTCGATg                                                                               <  2:557500/140‑1 (MQ=255)
                                                 acatTTGACTTAAATCACTACGTACACTCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGTTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATg                                                                               <  1:291924/140‑1 (MQ=255)
                                                       gACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCACCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAg                                                                         <  2:287748/141‑1 (MQ=255)
                                                        aCTTAAATCCCTACGTCCAATCGATACTAAGTCATTATCTGTATGATCATCACCCACAGTATCACCTGGTTTACCTAGATACGCACG‑ACTCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGt                                                                        <  2:161127/141‑1 (MQ=255)
                                                                ctctACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCgg                                                                >  1:382557/1‑141 (MQ=255)
                                                                  ctACGTATAATCGATANTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTa                                                              <  1:480829/141‑1 (MQ=255)
                                                                   tACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTaa                                                             >  1:365503/1‑141 (MQ=255)
                                                                     cGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGGGGTAATg                                                           >  1:395030/1‑141 (MQ=255)
                                                                       tACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTc                                                         >  2:212684/1‑141 (MQ=255)
                                                                          aaTCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGt                                                      >  2:36449/1‑141 (MQ=255)
                                                                          aaTCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGt                                                      <  1:554491/141‑1 (MQ=255)
                                                                          aaTCGATACTAATTCATTATCTGTATGAACATCAACCACAGTATCACCTGGTTTACCTAGATCCGCACG‑AATCAAAACCTTCCCTACCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGt                                                      <  1:198905/141‑1 (MQ=255)
                                                                               atgcTAATTCATTATCTGTATGATCATGAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCAc                                                 <  2:77528/138‑1 (MQ=255)
                                                                               aTACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCAc                                                 <  2:117475/141‑1 (MQ=255)
                                                                               aTACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCAc                                                 <  2:380902/141‑1 (MQ=255)
                                                                                   tAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTGATGTTTGTATCACTCGt                                             >  1:43384/1‑141 (MQ=255)
                                                                                          ttATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCa                                      >  2:384906/1‑141 (MQ=255)
                                                                                             tCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAAtt                                   <  1:436128/141‑1 (MQ=255)
                                                                                               tGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTAc                                 >  1:459579/1‑141 (MQ=255)
                                                                                                   tGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATAACTCGTTCTCGCAATTACGAAg                             >  1:232156/1‑141 (MQ=255)
                                                                                                    gATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGc                            >  2:247354/1‑141 (MQ=255)
                                                                                                      tcatcaACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTCATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCa                          <  2:234434/141‑1 (MQ=255)
                                                                                                        atcaACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCTCAATTACGAAGCCAGt                        <  1:338635/141‑1 (MQ=255)
                                                                                                        atcaACCACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGGATTAAATAGCCATTTTTTCGATTTCCAAGTACATGCGGTAATGTCTGTCTCCCTCGTTCTCGCACTTACAAAGCCAGt                        >  2:162971/1‑141 (MQ=255)
                                                                                                             ccACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACcat                   >  1:404640/1‑141 (MQ=255)
                                                                                                             ccACAGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATATCCACGTACATGCGGTAATGTCTGTATCACTCGTTCCCGCAATTACGAAGCCAGTACcac                   >  2:252121/1‑140 (MQ=255)
                                                                                                             ccACAGTATCACCTGGTTGACCTAGATACGCACG‑AATCAAATCCTTACCTTCCTGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACGCGTTCTCGCAATTACGAAGCCAGTACcat                   <  2:71851/141‑1 (MQ=255)
                                                                                                              cacaGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACcatc                  >  1:545740/1‑141 (MQ=255)
                                                                                                               acaGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATACGGTAATGTCTGTATCAATCGTTCTCGCAATTACGAAGCCAGTACcatca                 >  1:121935/1‑141 (MQ=255)
                                                                                                                 aGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATg               >  2:39338/1‑141 (MQ=255)
                                                                                                                 aGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATg               >  2:344150/1‑141 (MQ=255)
                                                                                                                 aGTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATg               >  2:48127/1‑141 (MQ=255)
                                                                                                                  gTATCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATgg              >  1:462531/1‑141 (MQ=255)
                                                                                                                     tCACCTGGTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTa           >  2:199910/1‑141 (MQ=255)
                                                                                                                      cACCTGGTTTACCTAGATACGCACG‑ATGCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACGTGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTAt          <  2:383738/141‑1 (MQ=255)
                                                                                                                            gTTTACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCaa    <  1:551936/141‑1 (MQ=255)
                                                                                                                              ttACCTAGATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATg  >  2:73094/1‑141 (MQ=255)
                                                                                                                                     |                                                                                                                                      
TTCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACG‑AATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATG  >  CP000730/1963635‑1963901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A