Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A19 F166 I0 R1	50	63.8	1389156	94.9%	1318309	140.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,744,473	C→T	100%	A67T (GCT→ACT)	apt ←	adenine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,744,473	0	C	T	100.0%	136.3 / ‑6.5	47	A67T (GCT→ACT)	apt	adenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (28/18); minor base G (0/1); total (28/19)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATAT  >  CP000730/1744337‑1744607

tGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAATCATTTGTACCATATTCTAGGTCTTACTCATAACGTATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGTTCCAAAGCCATTCCCCATTGACTAAGTTAcc                                                                                                                                    >  1:564334/1‑140 (MQ=255)

   ccTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTCGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCGTAGCCAATCCCGATTGACTAATTTACAGGa                                                                                                                                 >  1:261973/1‑141 (MQ=255)

   ccTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGa                                                                                                                                 <  1:154492/141‑1 (MQ=255)

    cTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGAc                                                                                                                                <  1:100696/141‑1 (MQ=255)

     tGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACa                                                                                                                               >  1:248699/1‑141 (MQ=255)

       gTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGc                                                                                                                             <  2:56258/141‑1 (MQ=255)

                       ttgCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCAcg                                                                                                             <  2:519911/140‑1 (MQ=255)

                          cATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACgcgc                                                                                                          <  1:530976/141‑1 (MQ=255)

                                    aCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATCAAGCCACGCGCTTCAGGTCCt                                                                                                >  1:204689/1‑141 (MQ=255)

                                    aCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTCAATAAGTTACAGGACAGCCAATGATAAAGCCACCCGCATCCGCTccc                                                                                                >  2:657973/1‑140 (MQ=255)

                                       tttGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACa                                                                                             >  1:638651/1‑141 (MQ=255)

                                        ttGTACTATATTCTAGGGCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAAGCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACaa                                                                                            <  2:59164/141‑1 (MQ=255)

                                         tGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTCCAAc                                                                                           >  2:667278/1‑141 (MQ=255)

                                           tACCATATTCTAGGTCATACTCATAACGAATGACTTCACGCGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGa                                                                                         >  2:395256/1‑141 (MQ=255)

                                           tACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCACTGATAAAGCCACGCGCTTCAGGTCCTACCACGa                                                                                         >  2:35116/1‑141 (MQ=255)

                                           tACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGCCCTACAACGa                                                                                         >  2:549543/1‑141 (MQ=255)

                                             ccATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATCAAGCCACGCGCTTCAGGTCCTACAACGata                                                                                       >  2:336142/1‑141 (MQ=255)

                                                   tCTAGGTCATACTCATAACGAATGACTTCGCGAGGTAATTTCCATCCTGTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACGGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAGAGATATCaaaa                                                                                 >  2:595747/1‑139 (MQ=255)

                                                      aGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGACAATGCCACGCGCTTCAGGTCCTACAACGATATCAACAtct                                                                              <  2:456795/141‑1 (MQ=255)

                                                       ggTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAtctc                                                                             >  2:569212/1‑141 (MQ=255)

                                                          cATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGt                                                                          >  1:198658/1‑141 (MQ=255)

                                                          cATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGt                                                                          >  1:658517/1‑141 (MQ=255)

                                                          cATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGt                                                                          >  1:462590/1‑141 (MQ=255)

                                                                       aaTGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTc                                                             <  2:638651/141‑1 (MQ=255)

                                                                        aTGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCACAGCCAATCCCCATTGGATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACATCGATATCAACATCTCTGTCTTTTGAGTATTcc                                                            >  1:610805/1‑140 (MQ=255)

                                                                          gACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTaa                                                          >  1:192297/1‑140 (MQ=255)

                                                                             ttCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGCTATCAACATCTCGGTCATTTGCGTATTCTCCAAt                                                       >  1:81634/1‑141 (MQ=255)

                                                                               cACGAGGTAATTTCCCTTCTTTTCTAACAGGGGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCGTCAGGTCCGATAACGATACCAACACCACCGTCTTTTGCGTATTCtgaaaatt                                                     <  2:496493/141‑8 (MQ=255)

                                                                                     gTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCtt                                               >  2:1897/1‑140 (MQ=255)

                                                                                      tAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGt                                              <  2:356617/141‑1 (MQ=255)

                                                                                       aaTTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGtt                                             <  1:581156/141‑1 (MQ=255)

                                                                                            cccTTCTTTTCTCACAGGTGCAAAGCCAATCCCCATTGAGTAAGTAACCGGACAGCCAATGATAAAGCCACGCCGTTCGGGTCCTACAACGATATCAACATCTCTGACTTCTGCGTATTCTAACATTTTATCTGTTGCATa                                        >  2:265865/1‑141 (MQ=255)

                                                                                            cccTTCTTTTCTAACAGGTGCGAGCTTAATTCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATa                                        >  1:686825/1‑141 (MQ=255)

                                                                                                  ttttCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCata                                  <  1:97989/141‑1 (MQ=255)

                                                                                                   tttCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCatat                                 >  1:587583/1‑141 (MQ=255)

                                                                                                    ttCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTCACCGGAGTGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATg                                <  1:190722/141‑1 (MQ=255)

                                                                                                      cTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCCTTTGCt                              >  2:38058/1‑141 (MQ=255)

                                                                                                          cagtcgcgaaGCCAATCCCCATGGAATAAGGTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCAc                          <  2:610805/133‑1 (MQ=255)

                                                                                                           aGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTTCATAGCCATATGCTTCAcc                         >  1:555824/1‑141 (MQ=255)

                                                                                                                  aaGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATcc                  >  2:482966/1‑141 (MQ=255)

                                                                                                                  aaGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATcc                  >  2:531103/1‑141 (MQ=255)

                                                                                                                  aaGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATATGTTGCATAGCCATATGCTTCACCATTATcc                  >  2:35488/1‑141 (MQ=255)

                                                                                                                    cccAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCAt                <  1:110208/140‑1 (MQ=255)

                                                                                                                      cAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATaa              <  1:403942/141‑1 (MQ=255)

                                                                                                                         tCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTg           <  1:569212/141‑1 (MQ=255)

                                                                                                                           cccATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTgta         <  1:438770/141‑1 (MQ=255)

                                                                                                                                  aaTAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAatat  <  1:395256/141‑1 (MQ=255)

TGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATAT  >  CP000730/1744337‑1744607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A