Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A19 F14 I0 R1	97	70.6	1554972	93.6%	1455453	141.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,744,473	C→T	100%	A67T (GCT→ACT)	apt ←	adenine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,744,473	0	C	T	100.0%	190.1 / NA	61	A67T (GCT→ACT)	apt	adenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (33/28); total (33/28)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATA  >  CP000730/1744335‑1744606

gTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTATAAAGCCAATCCCCATTGAGTAAGTTa                                                                                                                                     >  1:282337/1‑141 (MQ=255)

  tGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACa                                                                                                                                   >  2:70897/1‑141 (MQ=255)

  tGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGCTGCAAAGCCAATCCCCATTGAATAAGTTACa                                                                                                                                   >  1:187747/1‑141 (MQ=255)

     ccTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGa                                                                                                                                >  2:292151/1‑141 (MQ=255)

          tttAATTGCATCTTTGTGTATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACCTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGcc                                                                                                                           <  2:311144/141‑1 (MQ=255)

           ttAATTGCATTTTTGTGCATTGTTAAAACATTTGTACCATATCCTAGGTCATACTCATAACGAATGACCTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCa                                                                                                                          <  1:49065/141‑1 (MQ=255)

            tAATTTCATTTTTTTGCATTGTTAAAACATTTGTACCATTTTCAAGGTCATACTCATAACGAATGCCTTCACCAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCaa                                                                                                                         <  2:703932/141‑1 (MQ=255)

            tAATTGCATCTTTTGGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCaa                                                                                                                         <  2:406445/141‑1 (MQ=255)

            tAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCaa                                                                                                                         <  2:260323/141‑1 (MQ=255)

                        tgtgCATTGTTAAAACATTTGTACCATATTCTAGTTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCAc                                                                                                             >  1:182741/1‑141 (MQ=255)

                            cATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACgcgc                                                                                                         <  1:652118/141‑1 (MQ=255)

                               tGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAACCAATGATAAAGCAACGCGCTTc                                                                                                      >  2:181300/1‑141 (MQ=255)

                                      aCATTTGTACCATATTCTAGGTCATACTAATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCAAATGATAAAGCCACGCGCTTCAGGTCCt                                                                                               >  2:630680/1‑141 (MQ=255)

                                          ttGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACaa                                                                                           <  2:655626/141‑1 (MQ=255)

                                             tACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTTCAACGa                                                                                        >  1:311200/1‑141 (MQ=255)

                                                   atTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAACGCCACGCGCTTCAGGTCCTACCACGATACCaa                                                                                  >  2:365292/1‑141 (MQ=255)

                                                   atTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCTCTTCAGGTCCTACAACGATATCaa                                                                                  >  1:252622/1‑141 (MQ=255)

                                                      cTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAt                                                                               >  1:298792/1‑141 (MQ=255)

                                                        aGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAtct                                                                             <  1:696047/141‑1 (MQ=255)

                                                        aGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATCGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAtct                                                                             >  2:495902/1‑141 (MQ=255)

                                                         ggTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGTCAGCCAATGATAAAGCCACGCGCTTCTGGCCCTACAACGATATCAAAAtcta                                                                            >  1:437442/1‑140 (MQ=255)

                                                         ggTAATACTCATAACGAATGAATCACCGAGGTAATTTCCCTTCTCTTCTTACACAAACACAGCCCTCCCCCCTTCAATACGTAACAGCACATCCAACGAACACGCCACGCGCTCCAGGTCCTACAACGATATCACCAtccc                                                                            >  2:29406/1‑139 (MQ=255)

                                                           tCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTCCAACGATATCAACATTTCTg                                                                          >  1:168762/1‑141 (MQ=255)

                                                           tCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTg                                                                          <  1:292151/141‑1 (MQ=255)

                                                             aTACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTc                                                                        <  2:139531/141‑1 (MQ=255)

                                                                 tCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCGATTGAATAAGTTACAGGAGAGCCAATGATAAAGCCACGCGCTTCAGGTGCTAAAAGTATATAAACATGTCTGTCtttt                                                                    >  2:256569/1‑141 (MQ=255)

                                                                 tCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCAAAACCCATTTGAATAAGTTCCAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCACAGTCtttt                                                                    >  1:297362/1‑141 (MQ=255)

                                                                  cATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAGCAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGCACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTg                                                                   <  2:11163/141‑1 (MQ=255)

                                                                  cATAACGAATGACTTCACGAGGTAATGTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCATCAGGTCCTACAACGATATCAACATCTCTGTCTTTTg                                                                   >  2:481005/1‑141 (MQ=255)

                                                                   aTAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTCCAACGATATCAACATCTCTGTCttttcg                                                                  >  2:184928/1‑139 (MQ=255)

                                                                        gAATGACGTCACGAGATAATTTCCCTCCTTTTCTGACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATAATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAATATCTCTGTCTTTTGCGTAtt                                                             <  1:468678/141‑1 (MQ=255)

                                                                          gtgatgtCACGAGGTAATTTCCCTTCTGTTCTAACAGGTGCAAAGCCAAGCCCCATTGAATAAGTTACAGTACAGCCAATGATAAAGCCACGCGCTGCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCt                                                           <  2:589271/135‑1 (MQ=255)

                                                                          ctGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCt                                                           <  1:515991/140‑1 (MQ=255)

                                                                          aTGACTTCACGAGTTAATTTCCCTTCTTTTCTAACACGTGCAAAGCCAATCCCCGTTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTTCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCt                                                           <  1:432491/141‑1 (MQ=255)

                                                                           tGACTTCACGCGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTa                                                          <  2:49084/141‑1 (MQ=255)

                                                                                 cACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAAttt                                                    >  2:526270/1‑141 (MQ=255)

                                                                                 cACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAAttt                                                    >  2:574011/1‑141 (MQ=255)

                                                                                 cACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAAttt                                                    <  2:721753/141‑1 (MQ=255)

                                                                                   cGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGCACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTa                                                  <  2:285685/141‑1 (MQ=255)

                                                                                     aGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTTTTTTGCGTATTCTTCAAttttgtt                                                >  2:62972/1‑138 (MQ=255)

                                                                                      ggTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCt                                               >  1:285800/1‑141 (MQ=255)

                                                                                        tAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTAACTGt                                             <  1:526270/141‑1 (MQ=255)

                                                                                        gaaTTTCCCTTCTCTGCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGGCAGCCAATGATAAAGCCACGCGCTTCATGCCCTACAACGATATCCACATCTCTGGCTTTTGCGTATTCTACAATTTTATCTGt                                             <  2:4169/140‑1 (MQ=255)

                                                                                          aTTTCCCTTCTTTGCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACTACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTg                                           <  2:204123/141‑1 (MQ=255)

                                                                                              cccTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCCTa                                       >  1:248308/1‑141 (MQ=255)

                                                                                                      ttCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACCGCCCATGCTAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACACCCCTGGCCTTTGCTTATTCTACAATTTTATCTGTTGCATAGGCctatc                               >  1:251078/1‑140 (MQ=255)

                                                                                                          aaCCGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCTTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTc                           <  2:221046/141‑1 (MQ=255)

                                                                                                          aaCAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTTTTGCATGGCCATAttcttc                           >  1:349482/1‑141 (MQ=255)

                                                                                                          aaCAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTc                           <  1:165193/141‑1 (MQ=255)

                                                                                                          aaCAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTc                           <  1:70897/141‑1 (MQ=255)

                                                                                                           aCAGGTGCAAAGCGAATCCCCGTTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCa                          <  2:650423/141‑1 (MQ=255)

                                                                                                           aCAGGTGCAAAGCCAATGCCCACGGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCa                          <  2:728964/141‑1 (MQ=255)

                                                                                                               gTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCAt                      >  2:442789/1‑141 (MQ=255)

                                                                                                               gTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCGTTTGCATAGCCATATGCTTCACCAt                      >  2:607498/1‑141 (MQ=255)

                                                                                                                    aaGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATcc                 <  1:495902/141‑1 (MQ=255)

                                                                                                                    aaGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGAGAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATCCTTCACCATTATcc                 >  1:552170/1‑141 (MQ=255)

                                                                                                                     aGCCAATCCCCATTGAATAAGTTACAGTACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCa                <  2:493842/141‑1 (MQ=255)

                                                                                                                         aaTCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCAGCATTATCCATAAt            >  1:356469/1‑141 (MQ=255)

                                                                                                                           tCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAAttt          >  1:187750/1‑140 (MQ=255)

                                                                                                                                aTTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTTTCCATAATTttagta     >  1:308228/1‑141 (MQ=255)

                                                                                                                                   gAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATATTATCTGTTGCATAGCCATAGGCTGCCCCATTATCCATAATTGTAGTAata  >  2:512922/1‑141 (MQ=255)

GTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATA  >  CP000730/1744335‑1744606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A