breseq  version 0.32.1  revision 5f2cb669173e
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 11,042 A→G 7.6% V41V (GTA→GTG hutH → histidine ammonia‑lyase
RA 35,814 Δ1 bp 34.3% intergenic (+45/+63) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase
RA 35,817 A→T 35.2% intergenic (+48/+60) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase
RA 35,850 C→T 40.2% intergenic (+81/+27) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase
RA 35,858 T→A 44.1% intergenic (+89/+19) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase
RA 35,876 C→T 54.8% intergenic (+107/+1) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase
RA 35,913 C→G 21.3% S213S (TCG→TCC USA300HOU_0026 ← IS431mec transposase
RA 35,940 G→A 24.5% R204R (CGC→CGT USA300HOU_0026 ← IS431mec transposase
RA 35,970 T→C 63.1% E194E (GAA→GAG USA300HOU_0026 ← IS431mec transposase
RA 35,997 T→C 29.1% T185T (ACA→ACG USA300HOU_0026 ← IS431mec transposase
RA 36,015 C→T 28.0% R179R (AGG→AGA USA300HOU_0026 ← IS431mec transposase
RA 36,087 A→G 59.8% C155C (TGT→TGC USA300HOU_0026 ← IS431mec transposase
RA 36,119 C→T 31.1% V145I (GTA→ATA)  USA300HOU_0026 ← IS431mec transposase
RA 36,124 G→A 57.7% A143V (GCT→GTT)  USA300HOU_0026 ← IS431mec transposase
RA 36,133 A→G 31.6% V140A (GTA→GCA)  USA300HOU_0026 ← IS431mec transposase
RA 36,161 T→C 35.0% I131V (ATT→GTT)  USA300HOU_0026 ← IS431mec transposase
RA 36,174 T→C 34.0% K126K (AAA→AAG USA300HOU_0026 ← IS431mec transposase
RA 36,269 C→T 56.0% A95T (GCA→ACA)  USA300HOU_0026 ← IS431mec transposase
RA 36,315 G→A 56.1% Y79Y (TAC→TAT USA300HOU_0026 ← IS431mec transposase
RA 36,378 C→T 85.3% L58L (TTG→TTA USA300HOU_0026 ← IS431mec transposase
RA 36,413 C→T 54.8% V47I (GTC→ATC)  USA300HOU_0026 ← IS431mec transposase
RA 36,444 C→T 42.9% R36R (AGG→AGA USA300HOU_0026 ← IS431mec transposase
RA 36,471 A→G 28.3% S27S (AGT→AGC USA300HOU_0026 ← IS431mec transposase
RA 36,479 T→C 17.5% T25A (ACA→GCA)  USA300HOU_0026 ← IS431mec transposase
RA 36,581 C→T 40.4% intergenic (‑30/‑493) USA300HOU_0026 ← / → USA300HOU_0028 IS431mec transposase/hypothetical protein
RA 76,180 C→T 36.7% intergenic (+238/‑896) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,185 G→A 19.5% intergenic (+243/‑891) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,265 C→T 31.0% intergenic (+323/‑811) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,295 T→C 54.0% intergenic (+353/‑781) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,322 G→A 53.3% intergenic (+380/‑754) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,353 G→A 49.2% intergenic (+411/‑723) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 110,651 C→T 15.7% D170D (GAC→GAT USA300HOU_0109 → staphylococcal tandem lipoprotein
RA 110,660 T→A 27.0% I173I (ATT→ATA USA300HOU_0109 → staphylococcal tandem lipoprotein
RA 111,512 A→G 34.4% S194S (TCA→TCG USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 111,516 C→A 31.5% Q196K (CAA→AAA)  USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 293,940 C→T 25.0% intergenic (+57/‑471) USA300HOU_0261 → / → ispD2 zinc (Zn2+)‑binding dehydrogenase/2‑C‑methyl‑D‑erythritol 4‑phosphate cytidylyltransferase
RA 297,176 C→T 6.0% S337L (TCA→TTA)  tagB1 → glycosyl/glycerophosphate transferase
RA 478,502 C→A 100% D342E (GAC→GAA USA300HOU_0457 → NADH dehydrogenase (ubiquinone)
RA 479,096 T→C 5.5% F41F (TTT→TTC USA300HOU_0458 → hypothetical protein
RA 512,643 A→G 44.4% intergenic (+711/‑150) recR → / → rrs1 recombination protein RecR/16S ribosomal RNA
RA 538,142 T→C 9.6% F151F (TTT→TTC mfd → transcription‑repair coupling factor helicase
JC 567,301 (GGT)4→3 5.5% coding (196‑198/462 nt) ctsR → CtsR family transcriptional regulator
RA 584,121 G→T 100% intergenic (+205/‑67) rplA → / → rplJ ribosomal protein L1/ribosomal protein L10
RA 707,909 A→T 100% M1M (TTG→ATG) † USA300HOU_0653 ← ABC superfamily ATP binding cassette transporter, ABC protein
RA 737,162 A→G 7.6% R548R (AGA→AGG vraE → ABC superfamily ATP binding cassette transporter, membrane protein
RA 788,134 A→T 5.7% K100N (AAA→AAT pabC → possible aminodeoxychorismate lyase
RA 873,442 A→G 5.1% E116G (GAA→GGA)  clfA → fibrinogen‑binding protein
RA 973,213 C→A 100% E175* (GAG→TAG)  USA300HOU_0930 ← possible HAD superfamily hydrolase
RA 1,002,200 T→G 100% M173R (ATG→AGG)  mecA2 → competence negative regulator MecA
RA 1,105,994 T→C 100% T12T (ACT→ACC pdhD → dihydrolipoyl dehydrogenase
RA 1,135,045 G→A 5.3% R55C (CGT→TGT)  USA300HOU_1063 ← possible nucleotidyltransferase
RA 1,196,508 C→A 100% intergenic (+2563/‑1229) ftsQ → / → USA300HOU_1125 cell division protein FtsQ/hypothetical protein
RA 1,333,789 T→G 13.7% Y64D (TAT→GAT)  USA300HOU_1237 → GTP‑binding protein
RA 1,416,458 A→T 100% I216N (ATT→AAT)  USA300HOU_1314 ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
RA 1,513,930 A→T 100% L413F (TTA→TTT USA300HOU_1379 → hypothetical membrane protein
RA 1,532,200 G→A 6.9% R226C (CGT→TGT)  USA300HOU_1398 ← hypothetical membrane protein
RA 1,539,250 C→G 100% intergenic (‑349/+37) aroC ← / ← USA300HOU_1405 chorismate synthase/hypothetical protein
RA 1,646,189 G→C 100% A155G (GCA→GGA)  USA300HOU_1531 ← M24B family Xaa‑Pro dipeptidase
RA 1,744,473 C→T 100% A67T (GCT→ACT)  apt ← adenine phosphoribosyltransferase
RA 1,789,619 T→A 9.1% intergenic (‑100/+70) gapB ← / ← coaE glyceraldehyde‑3‑phosphate dehydrogenase (phosphorylating)/dephospho‑CoA kinase
RA 1,929,796 T→C 33.9% intergenic (‑879/‑289) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,797 G→A 33.9% intergenic (‑880/‑288) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,799 C→A 33.9% intergenic (‑882/‑286) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,804 A→G 36.1% intergenic (‑887/‑281) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,812 G→A 50.4% intergenic (‑895/‑273) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,842 Δ1 bp 100% intergenic (‑925/‑243) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,843 Δ1 bp 61.8% intergenic (‑926/‑242) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,848 C→A 63.3% intergenic (‑931/‑237) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,861 T→C 66.1% intergenic (‑944/‑224) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,863:1 +T 47.1% intergenic (‑946/‑222) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,864 A→T 17.3% intergenic (‑947/‑221) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,865 A→C 48.6% intergenic (‑948/‑220) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,865:1 +C 10.0% intergenic (‑948/‑220) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,866 G→T 64.1% intergenic (‑949/‑219) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,866:1 +T 2.9% intergenic (‑949/‑219) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,867 G→T 68.1% intergenic (‑950/‑218) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,868:1 +T 4.7% intergenic (‑951/‑217) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,870 A→C 67.9% intergenic (‑953/‑215) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,872 G→A 65.4% intergenic (‑955/‑213) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,873 C→A 66.7% intergenic (‑956/‑212) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,875 T→C 67.6% intergenic (‑958/‑210) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,917 C→T 58.8% intergenic (‑1000/‑168) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,918 A→G 58.2% intergenic (‑1001/‑167) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,925 T→A 59.6% intergenic (‑1008/‑160) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,928 T→A 59.8% intergenic (‑1011/‑157) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,930 T→A 59.3% intergenic (‑1013/‑155) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,941 A→T 50.3% intergenic (‑1024/‑144) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,946 G→A 43.4% intergenic (‑1029/‑139) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 2,010,363 C→T 100% C146Y (TGT→TAT)  USA300HOU_1861 ← ABC superfamily ATP binding cassette transporter, membrane protein
RA 2,024,507 C→A 10.1% A37E (GCA→GAA)  USA300HOU_1878 → hypothetical protein
RA 2,279,705 G→A 100% intergenic (+1373/+6659) mtlD → / ← glmM mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase
RA 2,337,959 C→T 100% intergenic (‑125/‑119) USA300HOU_2195 ← / → USA300HOU_2196 possible MerR family transcriptional regulator/hypothetical protein
RA 2,343,438 C→T 48.7% intergenic (+114/+250) USA300HOU_2200 → / ← alsD hypothetical protein/acetolactate decarboxylase
RA 2,455,692 Δ1 bp 100% coding (1210/1260 nt) USA300HOU_2315 → hypothetical membrane protein
RA 2,455,698 2 bp→AT 100% coding (1216‑1217/1260 nt) USA300HOU_2315 → hypothetical membrane protein
RA 2,532,298 A→T 100% T15T (ACA→ACT USA300HOU_2392 → FemAB family peptidoglycan biosynthesis protein
RA 2,728,368 A→G 4.7% M124V (ATG→GTG)  pyrD → dihydroorotate oxidase

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 512620 =84 (1.290)52 (1.160)
+40 bp
35/180 NT 47.3% intergenic (+688/‑173) recR/rrs1 recombination protein RecR/16S ribosomal RNA
?CP000730 1998278 = NA (NA)intergenic (‑6/+5) USA300HOU_t0050/rrf4 tRNA‑Val/5S ribosomal RNA
* ? CP000730 2082368 =82 (1.260)3 (0.050) 3/238 NT 3.9% intergenic (+9/+616) USA300HOU_1940/mapW2 aminotransferase/cell surface protein MapW2
?CP000730 2082406 = 73 (1.230)intergenic (+47/+578) USA300HOU_1940/mapW2 aminotransferase/cell surface protein MapW2