Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A21 F63 I0 R1	92	32.2	718920	92.2%	662844	140.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,539,250	C→G	100%	intergenic (‑349/+37)	aroC ← / ← USA300HOU_1405	chorismate synthase/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,539,250	0	C	G	97.6%	47.0 / ‑6.3	17	intergenic (‑349/+37)	aroC/USA300HOU_1405	chorismate synthase/hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (7/9); minor base A (0/1); total (7/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CATCATATAATCATTAATTAAAAATGATTAGCCGTTTTAATATAATCGTATCGCAACTTTAGCAAATAATATACTGACTACATTTCTTAATACAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAGTCCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAATATAATCAATTCTTTTACTAATACATAAAAAATATAAATGAGAGTCAGTATAAAAATAATTTTTGT  >  CP000730/1539118‑1539385

catcatATAATCATTAATTAAAAATGATTAGCCGTTTTAATATAATCGTATCGCAACTTTAGCAAATAATATACTGACTACATTTCTTAATACAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAGTGCGTTTaaa                                                                                                                                 >  2:160323/1‑141 (MQ=255)

                  taaAAATGATTAGCCGTTTTAATATAATCGTATCGCAACTTTAGCAAATAATATACTGACTACATTTCTTAATACAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATATTaaaa                                                                                                               >  1:282044/1‑141 (MQ=255)

                     aaaTGATTAGCCGTTTTAATATAATCGTATCGCAACTTTAGCAAATAATATACTGACTACATTTCTTAATACAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAatat                                                                                                            >  1:293640/1‑141 (MQ=255)

                                                                  taataTACTGACTACATTTCTTAATACAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAATATaa                                                               <  1:59325/141‑1 (MQ=255)

                                                                   aataTACTGACCACATTTCTTAATACAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAATATAAt                                                              <  1:294483/141‑1 (MQ=255)

                                                                             cTACATTTCTTAATACAAAAGAACGAACTTTTAATTATTAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAATATAATCAATTCtttt                                                    <  2:24803/141‑1 (MQ=255)

                                                                             cTACATTTCTTAATACAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAATATAATCAATTCtttt                                                    <  1:236001/141‑1 (MQ=255)

                                                                                  tttCTTAATACAAAAGAACAAACTGGTAATTATTAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGATAAGCGCTTTTGATATACTTTGACTTCAAAAATATAATCAATTCTTTTACTaa                                               >  1:180182/1‑141 (MQ=255)

                                                                                       tAATACAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAATATAATCAATTCTTTTACTAATACAt                                          <  1:83522/141‑1 (MQ=255)

                                                                                          aaaaaaaGAACGAACTTTTAAATATTAATAAAGTTAATCAGAAAGTATAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAATATAATCAATTCTTTTACTAATACATaaa                                       <  2:181081/138‑1 (MQ=255)

                                                                                            cAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAATATAATCAATTCTTTTACTAATACATaaaaa                                     >  2:222833/1‑141 (MQ=255)

                                                                                                         tGTTAATTATTAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAATATAATCAATTCTTTTACTAATACATAAAAAATATAAATGagag                        <  2:24589/141‑1 (MQ=255)

                                                                                                              attattAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAATATAATCAATTCTTTTACTAATACATAAAAAATATAAATgaacatcagt                   >  2:283866/1‑133 (MQ=255)

                                                                                                                    aataaAGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAATATAATCAATTCTTTTAAAAAAAAAAAAAAAATATAAATGAGAGTCAGTATaaaa             <  2:282044/141‑1 (MQ=255)

                                                                                                                      taaAGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAATATAATCAATTCTTTTACTAATACATAAAAAATTTAAATGAGAGTCAGTATAAAaat           >  1:197858/1‑141 (MQ=255)

                                                                                                                              aTCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAATATAATCAATTCTTTTACTAATACATAAAAAATATAAATGAGAGTCAGTATAAAAATAATTTTTg   <  2:10871/141‑1 (MQ=255)

                                                                                                                               tCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAATATAATCAATTCTTTTACTAATACATAAAAAATATAAATGAGAGTCAGTATAAAAATAATTTTTGt  <  2:202515/141‑1 (MQ=255)

CATCATATAATCATTAATTAAAAATGATTAGCCGTTTTAATATAATCGTATCGCAACTTTAGCAAATAATATACTGACTACATTTCTTAATACAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAGTCCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAATATAATCAATTCTTTTACTAATACATAAAAAATATAAATGAGAGTCAGTATAAAAATAATTTTTGT  >  CP000730/1539118‑1539385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A