Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F63 I0 R1	31	53.1	1128482	96.8%	1092370	140.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,744,454	C→T	100%	G73D (GGC→GAC)	apt ←	adenine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,744,454	0	C	T	100.0%	124.3 / ‑6.5	45	G73D (GGC→GAC)	apt	adenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (26/18); total (26/19)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCAT  >  CP000730/1744318‑1744586

atcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATACTAGGTCTTACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAAt                                                                                                                                  >  2:2708/1‑141 (MQ=255)

 tcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATc                                                                                                                                 <  2:75509/141‑1 (MQ=255)

        ttAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTg                                                                                                                          <  1:267439/141‑1 (MQ=255)

        ttAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTg                                                                                                                          <  1:267534/141‑1 (MQ=255)

         tAACACACGTTGACCTGATTTTGGCGCAGCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGa                                                                                                                         >  2:542743/1‑141 (MQ=255)

           acacacGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAAt                                                                                                                       <  2:102812/141‑1 (MQ=255)

            cacacGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACAAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATa                                                                                                                      >  1:236427/1‑141 (MQ=255)

             acacGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATaa                                                                                                                     >  1:544871/1‑141 (MQ=255)

                       cTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTGTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGAc                                                                                                           >  1:494493/1‑141 (MQ=255)

                                  gCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATa                                                                                                >  1:60854/1‑141 (MQ=255)

                                    aTCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGTATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTCCAGGACAGCCAATGATaaa                                                                                              >  1:74678/1‑141 (MQ=255)

                                    aTCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCCTTGAATAAGCTACAGGACAGCCAATGATaaa                                                                                              >  1:74677/1‑141 (MQ=255)

                                    aTCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCATTCTTTCTTAACAGATATAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATaaa                                                                                              >  2:170587/1‑141 (MQ=255)

                                         tgtgCATTGTTAAAACATTTGTACCAAATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGCTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCACAGAGAAAGCCAc                                                                                         >  1:536630/1‑141 (MQ=255)

                                                      aaCATTTCTGCCATATTCTAGGTCATACTCATAACGAATGGCTGCACGAGGTAATTTCCCTTCTTTTCTAACAGTTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACTGCCAATGATAAAGCCACGCGCTTCAGGTca                                                                            <  2:346748/141‑2 (MQ=255)

                                                       aCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCt                                                                           <  1:515141/141‑1 (MQ=255)

                                                        cATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAAGGTGAAAGCCACGCGCTTCAGGTCCTa                                                                          <  2:541850/141‑1 (MQ=255)

                                                         aTTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTAc                                                                         <  2:129930/141‑1 (MQ=255)

                                                         aTTGGTACCAGATTCTAGGGCATACTCATAACGAATGACTTCACGATGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCTATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTAc                                                                         <  2:87217/141‑1 (MQ=255)

                                                           ttGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACaa                                                                       >  2:492150/1‑141 (MQ=255)

                                                               aCCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGat                                                                   >  2:331801/1‑141 (MQ=255)

                                                                ccATATTCTAGGTCATACTCATAACGAATGACTACACGAGCTAATTTCCTTTCTATTCTAACAGGTGCAAAGTCAATCCCCAGTGAATAATCTACAGGACAGCCAATGATAAAGCCACTCGCATCAGGTACTACAGCGaga                                                                  >  2:370295/1‑139 (MQ=255)

                                                                         aGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTATAGCAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAAGGGTAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAtct                                                         >  1:324904/1‑141 (MQ=255)

                                                                          ggTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAtctc                                                        >  2:43303/1‑141 (MQ=255)

                                                                             cATACTCATAACGAATGACTTCACGAGGTAATTTTTTTTTTTTTTTTTTTTCTGCAAAGTAAGGCCCCATTGAATAAGCAAAATGACAGCCAATGATAAAGTCACGCGCTTCAGGTCCTTCACGGATATCACCATCTCTGt                                                     >  2:229614/1‑141 (MQ=255)

                                                                                    aTAACGAATGACTTCACGAGGTACTTTCCCTTCTTTTCTAACAGCTGCAAAGTCAATCACCATTGAATAAGCTACAGGACAGCCAATGCTAAAGCCACGCGCTTCAGGTCCTACAACGACATAACCATCTCTGTCTTTTGc                                              >  2:150150/1‑141 (MQ=255)

                                                                                    aTAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGTTATCAACATCTCTGTCTTTTGc                                              >  1:143803/1‑141 (MQ=255)

                                                                                         gAATGACTTCACGAGGTAATTTCCCTTTTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCtttttcatctt                                         >  1:496494/1‑134 (MQ=255)

                                                                                         gAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTATGTCTTTTGCGTAtt                                         >  1:496490/1‑141 (MQ=255)

                                                                                         gAATGACATCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAtt                                         >  1:353033/1‑141 (MQ=255)

                                                                                           aTGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCt                                       >  1:7280/1‑141 (MQ=255)

                                                                                             gACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTAc                                     >  1:40378/1‑141 (MQ=255)

                                                                                              aCTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACa                                    >  1:509691/1‑141 (MQ=255)

                                                                                              aCTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAACCCATTTTTAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACa                                    <  1:494586/141‑1 (MQ=255)

                                                                                                    cGAGGTAATTTCCCTTCTTTTCTAACCGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTa                              <  2:431246/141‑1 (MQ=255)

                                                                                                        gTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTg                          <  1:191302/141‑1 (MQ=255)

                                                                                                        gTAATTTCCCTTCTTTTCCAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTg                          <  2:74014/141‑1 (MQ=255)

                                                                                                         tAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGt                         <  1:439450/141‑1 (MQ=255)

                                                                                                         tAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGt                         <  2:544871/141‑1 (MQ=255)

                                                                                                                  ttcttTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGcc                <  1:170587/141‑1 (MQ=255)

                                                                                                                      tttCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGACATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCAAAGCCatat            >  2:29954/1‑141 (MQ=255)

                                                                                                                          tAACAGGTCCAAAGTCAATCCCCATGGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCtt        <  1:125931/141‑1 (MQ=255)

                                                                                                                            aCAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGCCAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGCCATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCa      <  1:518251/141‑1 (MQ=255)

                                                                                                                             cAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCAc     >  2:337933/1‑141 (MQ=255)

                                                                                                                                gTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCACTGGTAGAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTCATCTGTTGCATAGCCATATGCTTCACCAt  <  1:43303/141‑1 (MQ=255)

ATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCAT  >  CP000730/1744318‑1744586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A