Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F20 I0 R1	91	60.0	1338914	94.3%	1262595	140.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,744,454	C→T	100%	G73D (GGC→GAC)	apt ←	adenine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,744,454	0	C	T	100.0%	167.8 / ‑6.5	57	G73D (GGC→GAC)	apt	adenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (27/29); minor base A (1/0); total (28/29)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTA  >  CP000730/1744319‑1744588

tcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATc                                                                                                                                   <  1:354804/141‑1 (MQ=255)

      aTTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCAtt                                                                                                                             <  2:251205/141‑1 (MQ=255)

      aTTAACACACGGTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCAtt                                                                                                                             <  1:211076/141‑1 (MQ=255)

          acacacGTTGACCTGGTTTAATTGCCTCTTTGTGCGTTGTTAAAACATTTGTAGCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATGTCCCTTCTTTTCTAACAGGTGCAAAGACAATCCCCCTTgcac                                                                                                                         >  2:126446/1‑138 (MQ=255)

            acacGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATaa                                                                                                                       >  2:311544/1‑141 (MQ=255)

             cacGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAg                                                                                                                      >  1:181864/1‑141 (MQ=255)

              ccGTTGACCTGGTTTAATTGCATCTTCGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGc                                                                                                                     <  2:115413/140‑1 (MQ=255)

               cGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCt                                                                                                                    >  2:653478/1‑141 (MQ=255)

                gTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTa                                                                                                                   >  1:209922/1‑141 (MQ=255)

                   gACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAg                                                                                                                <  1:311544/141‑1 (MQ=255)

                    aCCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCCAAGTCAATCCCCATTGAATAAGCTACAgg                                                                                                               >  2:377413/1‑141 (MQ=255)

                            tAATTGCATCTTTGTGCATTGTTTAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCaa                                                                                                       <  1:302449/141‑1 (MQ=255)

                               ttGCATCTTTGAGCATTGTTAAAGCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGa                                                                                                    <  1:522480/141‑1 (MQ=255)

                                    tCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAg                                                                                               >  2:438320/1‑141 (MQ=255)

                                      tttGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGcc                                                                                             <  2:287920/141‑1 (MQ=255)

                                       ttGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCGTACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAGCAGGTGCAAAGTCAATCCACATTGAATAAGCTACAGGGCAGCCAGTGGTAAAGCCa                                                                                            >  1:610288/1‑141 (MQ=255)

                                         gtgCATTGTTAAAACATTTGTACCATCTTCTAGGTCATACTCATAACGAATGACTTCACGAGATAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATCAAGCCAcg                                                                                          >  1:265532/1‑141 (MQ=255)

                                          tgCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTGAATCCCCATTGAATAAGATCCAGGACAGCCAATGATAAAGCAAcca                                                                                         >  2:155358/1‑139 (MQ=255)

                                          tgCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCAcgc                                                                                         >  2:316877/1‑141 (MQ=255)

                                           gCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATCATAAAGCCAcgcg                                                                                        >  2:481221/1‑141 (MQ=255)

                                           gCATTGCTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTACAAAGTGACTCCCCAGTGGATAAGCTACCGGACAGCAAGTGATACAGCTCcgcg                                                                                        >  2:602987/1‑141 (MQ=255)

                                              ttGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCtt                                                                                     <  2:622260/141‑1 (MQ=255)

                                              ttGTTAAAACATTTGCACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCtt                                                                                     <  2:622322/141‑1 (MQ=255)

                                                 ttAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAg                                                                                  <  1:549823/141‑1 (MQ=255)

                                                     aaCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTcc                                                                              <  1:556910/141‑1 (MQ=255)

                                                            gTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACg                                                                       >  1:308024/1‑141 (MQ=255)

                                                                     tCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACa                                                              <  2:181864/141‑1 (MQ=255)

                                                                     tCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACa                                                              <  1:355301/141‑1 (MQ=255)

                                                                      cttgctCATACTCATAACGAACGACTTCACGCGTTATTTTCCCTTCTTTTCTAACAGGTGCGAAGTCAATCCCCATTGAAGAAGCTACAGGACAGCCAATGATATAGCCTCGCGCTTCAGGTCCTTCAACGATAGCAACAt                                                             <  2:175527/136‑1 (MQ=255)

                                                                      ctcggTCATGCTCATAACGAATGACTTCACGATGTCATTTCCCTTCTTTGCTAACAGGTGCAAAGTCAATCCCCTTTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAt                                                             <  2:610288/138‑1 (MQ=255)

                                                                      cTAGGTCATCCTCCTAACGAATGCCTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAt                                                             <  1:523829/141‑1 (MQ=255)

                                                                           tCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTg                                                        <  1:94210/141‑1 (MQ=255)

                                                                                 tCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATACCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACACGCTTCAGGTCCTACAACGATATCAACAGCTCTGACtttt                                                  >  2:514026/1‑141 (MQ=255)

                                                                                 tCATAACGAATGACTGCACGAGGTACTTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCAGTGACTAAGCTACAGGACAGCCAATGATAAAGCCCCGCGCTTCAGATCCTACAACGATATCAAccactcgacctttt                                                  >  2:2692/1‑128 (MQ=255)

                                                                                  cATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTAAATCCCAATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTg                                                 >  2:238601/1‑141 (MQ=255)

                                                                                   aTAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTATCAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGc                                                <  2:341285/141‑1 (MQ=255)

                                                                                     aaCGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACATGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGt                                              >  1:459580/1‑141 (MQ=255)

                                                                                       cGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAt                                            <  2:487948/141‑1 (MQ=255)

                                                                                           tGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTa                                        <  1:303898/141‑1 (MQ=255)

                                                                                            gccTTAACGAGGTAATTTCCCTTCTTTTCAAACAGAGGCAAAGTCAATCCTCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTAc                                       <  2:459580/139‑1 (MQ=255)

                                                                                             aCTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACa                                      >  2:450694/1‑141 (MQ=255)

                                                                                                 cACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGCATACGCACCAGGCCAGTCATTGATAAAGCCACTGTCTTTACGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAAttt                                  <  1:296954/141‑1 (MQ=255)

                                                                                                 cACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAAttt                                  <  1:592389/141‑1 (MQ=255)

                                                                                                    gAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCGACGCGCTTCAGGTCCTACAAGGATATAAACATCTTTGTGTTTTGCGTAGTCTACAATTTTAt                               >  1:287850/1‑141 (MQ=255)

                                                                                                    gAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCCCTGTCTTTTGCGTATTCTACAATTTTAt                               >  2:102594/1‑141 (MQ=255)

                                                                                                    gAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCACTTCCTACAACGATATCAACATCCCCGTCTATGCCGCACGCCACAATTTTAt                               >  1:138446/1‑141 (MQ=255)

                                                                                                      tgTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAGCTCTGTCTTTTGCGTATTCTACAATTTTATCt                             <  1:260447/140‑1 (MQ=255)

                                                                                                      ggTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAACCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTCGCGTATTATACAATTCGATCt                             >  1:33719/1‑141 (MQ=255)

                                                                                                      ggTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATACCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTACTACAACGATATCAACATCTCTGTCTTTTGCGTGTTCTACAATTCTATCt                             >  2:623429/1‑141 (MQ=255)

                                                                                                       gTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTTTTTTTTACTTGTTATATCATTTTATATg                            >  1:2176/1‑141 (MQ=255)

                                                                                                       gTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCtt                            >  1:447023/1‑140 (MQ=255)

                                                                                                        tAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGt                           <  1:438320/141‑1 (MQ=255)

                                                                                                           tttCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGTATAAACTACCGGACAGCCAGTGATAAAGCTGCCCGCAACAAGGAATGCAACGTAAACAACTCCGCGGTCTTTCGCGTATTCTCCAATTTTTTCTGtttc                        >  2:140173/1‑139 (MQ=255)

                                                                                                                       tCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAACGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTATACAATTTTATCTGTTGCATAGCCATATGc            >  2:491555/1‑141 (MQ=255)

                                                                                                                            cAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACACCGACATCAACATCTCCGTCCTTCGCGTCTTCTCCAATCTTATCTGTTGCACAGCCATATGCTTCAc       <  2:490459/141‑1 (MQ=255)

                                                                                                                            cAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCAc       <  1:537219/141‑1 (MQ=255)

                                                                                                                                 gCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTGTATGCAACATAGCCATATGCTTCACCATTa  <  2:308024/141‑1 (MQ=255)

TCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTA  >  CP000730/1744319‑1744588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A