Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F67 I0 R1	54	74.6	1619566	95.7%	1549924	141.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,454,626	A→T	100%	L48L (CTA→CTT)	USA300HOU_2315 →	hypothetical membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,454,626	0	A	T	100.0%	262.7 / NA	85	L48L (CTA→CTT)	USA300HOU_2315	hypothetical membrane protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (43/42); total (43/42)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTACCTTTAATTATTCT  >  CP000730/2454491‑2454763

aaaTAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCAcc                                                                                                                                      <  1:733519/140‑1 (MQ=255)

aaaTAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCAcc                                                                                                                                      <  1:624483/140‑1 (MQ=255)

 ataaaacTTGCTGGTTTTCAATGGGCAATGACGATTTTCTTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCa                                                                                                                                     <  2:700296/138‑1 (MQ=255)

 aaTAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTAACGATCTTGCACCa                                                                                                                                     >  2:141858/1‑141 (MQ=255)

  aTAAAGTTTCTGGTTCTCAATGCGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCAt                                                                                                                                    <  1:640256/141‑1 (MQ=255)

   tAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCAtt                                                                                                                                   >  2:43540/1‑141 (MQ=255)

   tAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCAtt                                                                                                                                   >  2:545954/1‑141 (MQ=255)

   tAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGAGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTAACAGATCTTGCACAAtt                                                                                                                                   >  2:541454/1‑141 (MQ=255)

     aaaTTGCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTTGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTaa                                                                                                                                 <  1:479424/141‑1 (MQ=255)

       aTTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAtg                                                                                                                               >  1:368570/1‑140 (MQ=255)

              gTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCaa                                                                                                                        <  1:401540/141‑1 (MQ=255)

                 ttCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCCGTCTATAATTGGTGTCAAACACTTTATATTTGAATTTAAAGATCTTGCACGATTAATTGATGGATTCa                                                                                                                     >  2:378267/1‑141 (MQ=255)

                 ttCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTACTTGCTGCAATCa                                                                                                                     >  2:78005/1‑141 (MQ=255)

                 ttCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCa                                                                                                                     >  2:260593/1‑141 (MQ=255)

                 ttCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCa                                                                                                                     >  2:726345/1‑141 (MQ=255)

                 ttCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCa                                                                                                                     >  2:327544/1‑141 (MQ=255)

                 ttCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCAATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTAAAGATCTTGCACCATTAATTGCTGCAATCa                                                                                                                     >  2:328428/1‑141 (MQ=255)

                    aaTGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCAttt                                                                                                                  <  1:648515/141‑1 (MQ=255)

                        ggCAATGACGATTTTCTTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTACTGCAATCATTTGtat                                                                                                              >  1:402171/1‑141 (MQ=255)

                             tGACGCTTTTCGTCTTCTTTGTCATTACAATGGCGTTAGCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTGGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCg                                                                                                         <  1:651014/141‑1 (MQ=255)

                             tGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATATTct                                                                                                         >  2:563482/1‑140 (MQ=255)

                             tGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCg                                                                                                         >  2:546820/1‑141 (MQ=255)

                              gACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGt                                                                                                        >  1:244596/1‑141 (MQ=255)

                                    tttCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGAAATCACTTGTATACTCGttttaca                                                                                                  >  1:679552/1‑138 (MQ=255)

                                      tCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAat                                                                                                <  1:119338/141‑1 (MQ=255)

                                       tgTCTGCTTTGGGATAAAAAGGGCGTTATCCATTATGCTCAAAAATTTCCAGTCTATAATTAGTGGCAAACCATTTATATTTGAAGGTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAata                                                                                               <  2:309318/140‑1 (MQ=255)

                                       ctgcttcttTTTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAata                                                                                               <  2:635125/138‑1 (MQ=255)

                                       cGTCTTCTTTGTCATTACTATTGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAata                                                                                               <  2:599681/141‑1 (MQ=255)

                                       cGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCACTCATTTGTATACTCGTTTTCAAata                                                                                               <  2:615923/141‑1 (MQ=255)

                                       cGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAata                                                                                               <  2:277977/141‑1 (MQ=255)

                                       cGTCTTCTTTGTCATCACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAata                                                                                               <  1:90681/141‑1 (MQ=255)

                                                   cATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCa                                                                                   >  1:692070/1‑141 (MQ=255)

                                                   cATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCa                                                                                   >  1:124543/1‑141 (MQ=255)

                                                      tACAATGGCGTTTTCCTTTATGCTCAGAGATTTCCAGTTTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATTTTGTACCATTAATTGCTGCACTCATTTGTATGTTGGTGTGCAAATATAAAAAGGTCCAAcg                                                                                <  2:174861/141‑2 (MQ=255)

                                                                ttATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTa                                                                      <  2:621859/141‑1 (MQ=255)

                                                                ttATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAAATTGCAGGTTTa                                                                      >  2:421621/1‑141 (MQ=255)

                                                                 tATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTaa                                                                     >  2:115354/1‑141 (MQ=255)

                                                                  aTCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTaaa                                                                    <  1:534671/141‑1 (MQ=255)

                                                                    ccATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAAt                                                                  >  2:105787/1‑141 (MQ=255)

                                                                            cTCAGAGAGTGCCAGGCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATc                                                          <  1:131381/141‑1 (MQ=255)

                                                                                     ttCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGaaa                                                 >  2:185651/1‑141 (MQ=255)

                                                                                     ttCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGAACTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCCACTTGCAGGTTTAAAACTCTCAATCAGCCTGaaa                                                 >  2:518975/1‑141 (MQ=255)

                                                                                     ctCCAGTCTATAATTGGTTTCGAACACTTTATATTTGAAGTTGCAGATCTTGCACCATTAATGGCTGCCATCATTTGTATACTCGATTTCATATATAAAAAGGTCCGACTTGCAGGTTTAAAATTCTCAATCAGccgggaa                                                 >  2:255897/2‑136 (MQ=255)

                                                                                      tCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCCCCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTACAGGTTTAAAATTCTCAATCAGCATGAAAg                                                >  2:667883/1‑141 (MQ=255)

                                                                                      tCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAg                                                <  2:550377/141‑1 (MQ=255)

                                                                                      tCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCGGAAAg                                                >  2:336855/1‑141 (MQ=255)

                                                                                      tCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATGGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAg                                                >  2:708987/1‑141 (MQ=255)

                                                                                      accAGTCTATAATTGGTGTCAAACACTTTATACTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAACCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTCGCAGGTTTAAAATTCTCAATCAGCCTGAAAg                                                <  2:408166/140‑1 (MQ=255)

                                                                                        cAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTa                                              <  1:188266/141‑1 (MQ=255)

                                                                                        cAGTCTATAATTGAGGTCAAACACTCTATAATTGAAGCTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTa                                              <  1:32315/141‑1 (MQ=255)

                                                                                          gTCTATAATTGGTGTCAAACCCTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTGTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAAt                                            <  1:153394/141‑1 (MQ=255)

                                                                                          gTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAACAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAAt                                            >  2:131982/1‑141 (MQ=255)

                                                                                          gTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGTAAGTAAt                                            >  2:684948/1‑141 (MQ=255)

                                                                                          atCTATAATTGGTGTCAAACTCTTTATATTTGAAGTTACAGTTCTTTCACCATTAATTGCTGTTATCATTTGTATTCTCGTTTTCAAATATAAAAAGGTCCATCTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAAt                                            <  2:756210/140‑1 (MQ=255)

                                                                                           tCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAAtt                                           <  2:223869/141‑1 (MQ=255)

                                                                                           tCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAAtt                                           <  2:683607/141‑1 (MQ=255)

                                                                                             tataATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGa                                         <  2:244596/141‑1 (MQ=255)

                                                                                                      tgtCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAGTGCTGCAATCATTTGTATACTCGTTTTCAAATATAATAAGGTCCTACTTGCAGGTTTAAAATTCTCAATCAACCTGAAAGTAATTGAACGTCTAtt                                >  2:351785/1‑141 (MQ=255)

                                                                                                        tCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGc                              <  1:558605/141‑1 (MQ=255)

                                                                                                           aaCACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAg                           >  1:56337/1‑141 (MQ=255)

                                                                                                           aaCACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATAGAACGTCTATTGCAAg                           >  2:301295/1‑141 (MQ=255)

                                                                                                            acacTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACg                                      <  2:329551/129‑1 (MQ=255)

                                                                                                            acacTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACg                                      >  1:329551/1‑129 (MQ=255)

                                                                                                             cacTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAACCGTCTCAGTCATCCTGAAAGTAATTGAACGTCTATTGCTAGCt                         >  1:752637/1‑141 (MQ=255)

                                                                                                               cTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCttt                       <  1:335718/141‑1 (MQ=255)

                                                                                                                   atatTTGAAGTTACAGATCTTGCACCATTGATTGTTGCAATCATGTTTATACGCTTTTTCGAGTTTAAAAAGGTCCAACTTGCAGGTTTAAAATTGTCAATCAGCGTGCAAGTAAGTGAAGGTCCATTGCTAGCCTTAAtt                   >  2:193093/1‑141 (MQ=255)

                                                                                                                        tGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTGCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTAcc              <  2:446455/141‑1 (MQ=255)

                                                                                                                         gAAGTTACAGATCTTGCGCCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTACCt             >  2:800781/1‑141 (MQ=255)

                                                                                                                         gAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAGATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTACCt             >  1:350532/1‑141 (MQ=255)

                                                                                                                         gAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCACTCAGCCTGAAAGTAATTGAACGTATATTGCTAGATTTAATTTTACCt             >  1:54488/1‑141 (MQ=255)

                                                                                                                         gAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACTTCTATTGCTAGCTTTAATTTTCCCt             >  1:229425/1‑141 (MQ=255)

                                                                                                                         gAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTACCt             >  2:692767/1‑141 (MQ=255)

                                                                                                                          aaGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCATGCAAGTAATCGAACGTCTATTGCTAGCCTTAATTTTACCtt            <  2:676004/141‑1 (MQ=255)

                                                                                                                           aagtcctgATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTGAAATATAAAAAGGTCCAACTTGCAGGTTTCGAATGCTCAATCAGCCTGAAAGTAACTGAACGTCTATTGCTAGCTTTAATTTTACCttt           <  1:352534/134‑1 (MQ=255)

                                                                                                                           aGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTACCttt           <  1:210555/141‑1 (MQ=255)

                                                                                                                           aGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTACCttt           <  2:500973/141‑1 (MQ=255)

                                                                                                                            gTTACAGATCTTGCAGCATTAATTGCCGCAATCACTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAACATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGTTAGCTTTAATTTTACCTTTa          <  1:490738/141‑1 (MQ=255)

                                                                                                                            gTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGCACGTCTATTGCTCGCTTTAATTTTACCTTTa          >  2:8604/1‑141 (MQ=255)

                                                                                                                            gTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTACCtttt          >  2:763045/1‑140 (MQ=255)

                                                                                                                            gTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTACCTTTa          <  1:424832/141‑1 (MQ=255)

                                                                                                                               aCAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTACCTTTAatt       >  1:665981/1‑141 (MQ=255)

                                                                                                                                  gATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTACCTTTAattatt    <  1:728120/141‑1 (MQ=255)

                                                                                                                                   aTCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTACCTTTAATTATTc   <  1:233675/141‑1 (MQ=255)

                                                                                                                                    tCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTACCTTTAATTATTCt  <  2:124543/141‑1 (MQ=255)

                                                                                                                                    tCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTACCTTTAATTATTCt  <  2:692070/141‑1 (MQ=255)

CAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTACCTTTAATTATTCT  >  CP000730/2454491‑2454763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A