breseq  version 0.32.1  revision 5f2cb669173e
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 66,465 T→C 5.6% R14R (AGA→AGG USA300HOU_0062 ← hypothetical protein
RA 76,969 G→A 100% intergenic (+1027/‑107) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 94,785 A→G 6.9% T212T (ACA→ACG USA300HOU_0093 → hypothetical protein
RA 96,144 A→G 8.6% intergenic (+660/+819) USA300HOU_0093 → / ← USA300HOU_0095 hypothetical protein/hypothetical protein
RA 110,651 C→T 10.5% D170D (GAC→GAT USA300HOU_0109 → staphylococcal tandem lipoprotein
RA 110,660 T→A 13.0% I173I (ATT→ATA USA300HOU_0109 → staphylococcal tandem lipoprotein
RA 111,512 A→G 42.8% S194S (TCA→TCG USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 111,516 C→A 40.0% Q196K (CAA→AAA)  USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 111,560 T→C 9.9% R210R (CGT→CGC USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 195,286 T→A 100% intergenic (‑97/‑82) USA300HOU_0183 ← / → USA300HOU_0184 hypothetical protein/hypothetical protein
RA 312,003 A→G 5.8% K165E (AAA→GAA)  bglA → 6‑phospho‑beta‑glucosidase
RA 396,540 G→A 100% A208T (GCA→ACA)  USA300HOU_0364 → possible lipoprotein
RA 426,904 T→A 4.3% E140V (GAA→GTA)  USA300HOU_0401 ← hypothetical protein
RA 456,707 T→A 6.1% S88R (AGT→AGA set25 → staphylococcal exotoxin
RA 512,643 A→G 52.6% intergenic (+711/‑150) recR → / → rrs1 recombination protein RecR/16S ribosomal RNA
RA 607,884 G→A 100% R209* (CGA→TGA)  USA300HOU_0550 ← deoxynucleoside kinase
RA 629,681 C→A 100% L84I (CTA→ATA)  USA300HOU_0564 → possible 3‑hexulose‑6‑phosphate synthase
RA 637,932 G→A 5.1% V241V (GTC→GTT thiD ← phosphomethylpyrimidine kinase
RA 665,392 T→G 100% L79* (TTA→TGA)  USA300HOU_0608 → possible acetytransferase
RA 667,735 A→C 5.4% intergenic (+767/‑281) USA300HOU_0609 → / → adh phosphohydrolase/alcohol dehydrogenase
RA 707,910 A→G 100% intergenic (‑1/‑121) USA300HOU_0653 ← / → USA300HOU_0654 ABC superfamily ATP binding cassette transporter, ABC protein/possible metal‑dependent transcriptional regulator
RA 712,088 T→C 7.9% L134S (TTA→TCA)  tagG → teichoic acid ABC superfamily ATP binding cassette transporter, membrane protein
RA 835,012 G→T 6.9% M229I (ATG→ATT prfB → peptide chain release factor 2
RA 903,873 A→G 6.3% intergenic (+148/‑523) USA300HOU_0861 → / → USA300HOU_0862 possible DNA primase/hypothetical protein
RA 1,210,010 G→C 4.8% E146Q (GAA→CAA)  pyrB → aspartate carbamoyltransferase
RA 1,233,504 T→A 100% V470D (GTT→GAT)  USA300HOU_1157 → non‑specific serine/threonine protein kinase
RA 1,416,458 A→T 100% I216N (ATT→AAT)  USA300HOU_1314 ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
RA 1,435,069 A→C 23.2% T142T (ACA→ACC dapD → 2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑succinyltransferase
RA 1,438,346 C→T 6.2% Q204* (CAG→TAG)  lysA2 → diaminopimelate decarboxylase
RA 1,440,085 G→T 100% intergenic (‑45/‑891) cspA ← / → xpaC cold shock protein CspA/5‑bromo‑4‑chloroindolyl phosphate hydrolysis protein XpaC
RA 1,507,565 G→A 6.3% A4V (GCA→GTA)  ald1 ← alanine dehydrogenase
RA 1,513,930 A→T 100% L413F (TTA→TTT USA300HOU_1379 → hypothetical membrane protein
RA 1,646,189 G→C 100% A155G (GCA→GGA)  USA300HOU_1531 ← M24B family Xaa‑Pro dipeptidase
RA 1,677,731 A→G 5.5% E290G (GAG→GGG)  glyS → glycine‑‑tRNA ligase
RA 1,744,362 G→A 100% H104Y (CAC→TAC)  apt ← adenine phosphoribosyltransferase
RA 1,761,889 C→A 100% D388Y (GAT→TAT)  folC ← tetrahydrofolate synthase
RA 1,783,940 T→C 6.6% M381V (ATG→GTG)  thrS ← threonine‑‑tRNA ligase
RA 1,794,352 A→T 5.4% Y431N (TAT→AAT)  USA300HOU_1678 ← hypothetical membrane protein
RA 1,857,700 A→G 7.3% I225T (ATA→ACA)  USA300HOU_1728 ← hypothetical protein
RA 1,874,676 T→C 5.4% E178G (GAA→GGA)  rsuA ← pseudouridylate synthase
RA 1,963,768 C→G 100% V365L (GTA→CTA)  hemG ← protoporphyrinogen oxidase
RA 2,010,363 C→T 100% C146Y (TGT→TAT)  USA300HOU_1861 ← ABC superfamily ATP binding cassette transporter, membrane protein
RA 2,018,834 G→C 11.2% P29A (CCA→GCA)  USA300HOU_1869 ← transglycosylase
RA 2,026,958 C→T 5.2% G330D (GGT→GAT)  vraS ← sensor histidine kinase VraS
RA 2,186,126 G→T 7.1% S12* (TCA→TAA)  fliA ← DNA‑directed RNA polymerase sigma subunit FliA
RA 2,279,705 G→A 100% intergenic (+1373/+6659) mtlD → / ← glmM mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase
RA 2,352,815 G→A 100% A92V (GCA→GTA)  rpsI ← ribosomal protein S9
RA 2,399,409 C→G 4.8% E154Q (GAA→CAA)  USA300HOU_2263 ← acetyltransferase
RA 2,454,626 A→T 100% L48L (CTA→CTT USA300HOU_2315 → hypothetical membrane protein
RA 2,458,481 T→C 6.5% R32R (AGA→AGG USA300HOU_2319 ← aldose 1‑epimerase
RA 2,532,298 A→T 100% T15T (ACA→ACT USA300HOU_2392 → FemAB family peptidoglycan biosynthesis protein
RA 2,707,179 A→G 5.3% F51F (TTT→TTC USA300HOU_2559 ← phytoene dehydrogenase
RA 2,775,053 A→G 6.6% D711D (GAT→GAC clfB ← clumping factor B
RA 2,857,614 T→G 5.5% intergenic (+10/+55) USA300HOU_2699 → / ← USA300HOU_2700 NiCoT family nickel (Ni2+)‑cobalt (Co2+) transporter/hypothetical protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 512620 =60 (0.860)47 (1.040)
+45 bp
28/170 NT 55.0% intergenic (+688/‑173) recR/rrs1 recombination protein RecR/16S ribosomal RNA
?CP000730 2176447 = 58 (0.830)noncoding (114/114 nt) rrf5 5S ribosomal RNA
* ? CP000730 514600 =NA (NA)3 (0.050) 3/246 NT NA intergenic (+268/‑106) rrs1/rrl1 16S ribosomal RNA/23S ribosomal RNA
?CP000730 514618 = NA (NA)intergenic (+286/‑88) rrs1/rrl1 16S ribosomal RNA/23S ribosomal RNA
* ? CP000730 2085296 =64 (0.920)31 (0.500) 24/232 NT 36.5% intergenic (‑558/‑400) mapW2/USA300HOU_1944 cell surface protein MapW2/hypothetical protein
?CP000730 = 2128343 51 (0.820)intergenic (+57/+1062) int/USA300HOU_2011 bacteriophage integrase/possible leukocidin subunit