Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F52 I1 R1	32	41.0	931009	90.8%	845356	141.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	396,851	G→A	G28S (GGT→AGT)	ywbN →	iron (Fe2+)‑dependent Dyp family peroxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	396,851	0	G	A	100.0%	137.2 / NA	47	G28S (GGT→AGT)	ywbN	iron (Fe2+)‑dependent Dyp family peroxidase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (28/19); total (28/19)

GATGCTGTTAATGCATTAGGAGAGCCACTAAGTAAAATGGCTGTGATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCGGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGTTCAATACACCAGAAGATCCGGAAAAAGATGCGTATGAATTTTATGGTAAAGTGCAACCAGGCATTACCACACCCACGCAA  >  CP000730/396717‑396985

gATGCTGTTAATGCATTAGGAGAGCCACTAAGTAAAATGGCTGTGATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTg                                                                                                                                  >  1:618972/1‑141 (MQ=255)

  tGCTGTTAATGCATTAGGAGAGCCACTAAGTAAAATGGCTGTGATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCa                                                                                                                                >  1:314216/1‑141 (MQ=255)

      gTTAATGCATTAGGAGAGCCACTAAGTAAAATGGCTGTGATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTg                                                                                                                            >  1:196084/1‑141 (MQ=255)

       ttAATGCATTAGGAGAGCCACTAAGTAAAATGGCTGTGATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCGATTgg                                                                                                                           >  1:232363/1‑141 (MQ=255)

       ttAATGCATTAGGAGAGCCACTAAGTAAAATGGCTGTGATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTgg                                                                                                                           >  1:796642/1‑141 (MQ=255)

       ttAATGCATTAGGAGAGCCACTAAGTAAAATGGCTGTGATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTgg                                                                                                                           >  1:385746/1‑141 (MQ=255)

       ttAATGCATTAGGAGAGCCACTAAGTAAAATGGCTGTGATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTAGCAATTgg                                                                                                                           >  1:188684/1‑141 (MQ=255)

                 aGGAGAGCCACTAAGTAAAATGGCTGTGATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTCAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAgtggt                                                                                                                 <  1:874827/141‑1 (MQ=255)

                 aGGAGAGCCACTAAGTAAAATGGCTGTGATTACTGAATGACAAATTATGAACAAGTTAAAGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAgtggt                                                                                                                 <  1:65370/141‑1 (MQ=255)

                   gagaGCCACTAAGTAAAATGGCTGTGATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGCCGGTGCCAGTGTTGCAATTGGCGCAAGTGgcgt                                                                                                               >  1:713807/1‑141 (MQ=255)

                        ccACTAAGTAAAATGGCTGTGATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTa                                                                                                          >  1:375291/1‑141 (MQ=255)

                              aGTAAAATGGCGGTGATTACCGAACGACAAATTATAAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATCGGCGCTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCAtgt                                                                                                    <  1:30576/141‑1 (MQ=255)

                               gTAAAATGGCTGTGATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCAtgtg                                                                                                   <  1:125228/141‑1 (MQ=255)

                               gTAAAATGGCTGTGATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCAtgtg                                                                                                   <  1:634282/141‑1 (MQ=255)

                                   aaTGGCTGTGATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCt                                                                                               <  1:135684/141‑1 (MQ=255)

                                       gCTGTGATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTGGCATGTGGTCTTTCa                                                                                           >  1:846587/1‑141 (MQ=255)

                                       gCTGTGATTACTGAATGACAAATTAGGAATAAGTTAGCGATAGTACGCAATCTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCa                                                                                           <  1:671299/141‑1 (MQ=255)

                                        cTGTGATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCCATTGGGGCAAGTGGTGTTGGTAGCATGTGGTCTTTCaa                                                                                          >  1:210907/1‑141 (MQ=255)

                                        cTGTGATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCaa                                                                                          <  1:408404/141‑1 (MQ=255)

                                         tgtgATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTAGATTTTTGAAAATGTTAGGTATTGGAGGTGCCAGTGTTGCGATTGGCGCAACTGGTGTTAGTAGCATGTGGTCTTTCaaa                                                                                         <  1:599670/141‑1 (MQ=255)

                                         tgtgAATACCGAAGCACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCaaa                                                                                         <  1:411079/141‑1 (MQ=255)

                                           tgATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATc                                                                                       <  1:813511/141‑1 (MQ=255)

                                           tgATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATc                                                                                       <  1:163405/141‑1 (MQ=255)

                                              ttACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTATTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAACTCAAt                                                                                    >  1:734963/1‑141 (MQ=255)

                                                aCTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGt                                                                                  >  1:361237/1‑141 (MQ=255)

                                                aCTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGt                                                                                  <  1:800171/141‑1 (MQ=255)

                                                aCTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATAAATGt                                                                                  >  1:649318/1‑141 (MQ=255)

                                                 cTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGtt                                                                                 >  1:707716/1‑141 (MQ=255)

                                                          aaaTTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGTTCAATACAcc                                                                        >  1:356852/1‑141 (MQ=255)

                                                                   aCAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGTTCAATACACCAGAAGATcc                                                               >  1:804620/1‑141 (MQ=255)

                                                                          aaCGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCACTGTTCAATACACCAGAAGATCCGGaaaaa                                                        >  1:15378/1‑141 (MQ=255)

                                                                           aCGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGTTCAATACCCCAGAAGATCCGGAAAAAg                                                       >  1:113569/1‑141 (MQ=255)

                                                                           aCGATAGTACGCAATTTTCAAGACGGACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGTTCAATACACCAGAAGATCCGGAAAAAg                                                       <  1:271341/141‑1 (MQ=255)

                                                                                aGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGTTCAATACAACAGAAGATCAGGAAAAAGATGCg                                                  >  1:616519/1‑141 (MQ=255)

                                                                                aGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGATATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCACATCAATGTTCAATACACGAGAAGGGCCGGAAAAAGATGCg                                                  >  1:229141/1‑141 (MQ=255)

                                                                                    cGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGTTCAATACACCAGTATATCCGGAAAAAGATGCGTATg                                              <  1:542703/141‑1 (MQ=255)

                                                                                          tttCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAACACGATGTTCAATACACCAGAAGATCCGGAAAAAGATGCGTATGACtttt                                        >  1:123695/1‑141 (MQ=255)

                                                                                          tttCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGTTCAATACACAGGAAGGTCAGGAAAAAGATGCGTATGAAtttt                                        >  1:886746/1‑141 (MQ=255)

                                                                                              aaGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGTTCAATACACCAGAAGATCCGGAAAAAGATGCGTATGAATTTTATgg                                    >  1:135739/1‑141 (MQ=255)

                                                                                                         ttttGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGTTCAATACACCAGAAGATCCGGAAAAAGATGCGTATGAATTTTATGGTAAAGTGCAAc                         <  1:702672/141‑1 (MQ=255)

                                                                                                         tgttGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGTTCAATACACCAGAAGATCCGGAAAAAGATGCGTATGAATTTTATGGTAAAGTGCAAc                         <  1:299432/139‑1 (MQ=255)

                                                                                                            tGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGTTCAATACACCAGAAGATCCGGAAAAAGATGCGTATGAATTTTATGGTAAAGTGCAACCAg                      <  1:74059/141‑1 (MQ=255)

                                                                                                                      aGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGTTCAATACACCAGAAGATCCGGAAAAAGATGCGTATGAATTTTATGGTAAAGTGCAACCAGGCATTACcac            >  1:431639/1‑141 (MQ=255)

                                                                                                                      aGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGTTCAATACACCAGAAGATCCGGAAAAAGATGCGTATGAATTTTATGGTAAAGTGCAACCAGGCATTACcac            >  1:616923/1‑141 (MQ=255)

                                                                                                                            tGGCGGGGCCAGTGTTGAAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTATTTTAATTCAATGGTCAATACACCAGAAGATCCGGAAAAAGATGCGTATGAATTTTAAGGTAAAGTGCAACCAGGCATTACCACACCCAc      <  1:212977/141‑1 (MQ=255)

                                                                                                                              gCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGTTCAATACACCAGAAGATCCGGAAAAAGATGCGTATGAATTTTATGGTAAAGTGCAACCAGGCATTAccacgcccacgc    >  1:182555/1‑141 (MQ=255)

                                                                                                                                ggTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAAGGTTCAATACACCAGAAGATCCGGAAAAAGATGCGTATGAATTTTATGGTAAAGTGGAACCAGGCATTACCACACCCACGCaa  >  1:559890/1‑141 (MQ=255)

GATGCTGTTAATGCATTAGGAGAGCCACTAAGTAAAATGGCTGTGATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCGGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGTTCAATACACCAGAAGATCCGGAAAAAGATGCGTATGAATTTTATGGTAAAGTGCAACCAGGCATTACCACACCCACGCAA  >  CP000730/396717‑396985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A