Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A17 F172 I1 R2	29	34.5	745914	96.0%	716077	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,709,297	T→A	K544* (AAA→TAA)	oatA ←	possible acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,709,297	0	T	A	100.0%	78.6 / NA	24	K544* (AAA→TAA)	oatA	possible acetyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (11/13); total (11/13)

GCGCTTTACTACCTGCATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTTATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACA  >  CP000730/2709168‑2709427

gcgcTTTACTACCTGCATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTAc                                                                                                                         >  2:136021/1‑141 (MQ=255)

  gcTTTACTACCTGCATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCt                                                                                                                       <  2:134715/141‑1 (MQ=255)

     ttACTACCTGCATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCa                                                                                                                    <  2:189170/141‑1 (MQ=255)

        ctacCTGCATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATaa                                                                                                                 >  1:191899/1‑141 (MQ=255)

                 tatTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGt                                                                                                        <  2:87267/141‑1 (MQ=255)

                           gAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAg                                                                                              >  2:252920/1‑141 (MQ=255)

                                   tCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTaaa                                                                                      <  2:298025/141‑1 (MQ=255)

                                            aaGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCt                                                                             >  1:280532/1‑141 (MQ=255)

                                                  tCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCtttt                                                                       >  1:172456/1‑141 (MQ=255)

                                                    cGGATGACCTGCAGAAGCTTTATACCAATCGAATAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTcc                                                                     <  2:59238/141‑1 (MQ=255)

                                                           ccTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTa                                                              >  1:69010/1‑141 (MQ=255)

                                                                    gCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGt                                                     >  1:260879/1‑141 (MQ=255)

                                                                     cTTTATACCAATCGACTAGATGTACAATAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCGTAATCTCTAGGTACTCTAATAGATACTATACATATGTCTGCTTTTCCAAAACTATCCAATAGtt                                                    <  2:109317/141‑1 (MQ=255)

                                                                          taCCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCAttt                                               >  2:102250/1‑141 (MQ=255)

                                                                             cAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAAt                                            <  2:167927/141‑1 (MQ=255)

                                                                             cAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAAt                                            <  2:156317/141‑1 (MQ=255)

                                                                              aaTCGACTATATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAAtt                                           <  1:74863/141‑1 (MQ=255)

                                                                                              aTTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGc                           >  1:172658/1‑141 (MQ=255)

                                                                                                tAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGccc                         >  2:346648/1‑141 (MQ=255)

                                                                                                 agagCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGcccc                        >  1:310869/1‑141 (MQ=255)

                                                                                                    gcgcTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCAtt                     <  2:286239/141‑1 (MQ=255)

                                                                                                         tttCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTAc                <  1:19346/141‑1 (MQ=255)

                                                                                                         tttCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTAc                <  1:98475/141‑1 (MQ=255)

                                                                                                                       tAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACa  <  1:97764/141‑1 (MQ=255)

GCGCTTTACTACCTGCATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTTATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACA  >  CP000730/2709168‑2709427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A