Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F22 I0 R1	151	71.0	1572916	92.9%	1461238	140.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,455,692	Δ1 bp	10.4%	coding (1210/1260 nt)	USA300HOU_2315 →	hypothetical membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,455,692	0	G	.	10.4%	246.9 / 24.4	77	coding (1210/1260 nt)	USA300HOU_2315	hypothetical membrane protein
Reads supporting (aligned to +/- strand): ref base G (36/32); new base . (5/3); total (42/35)
Fisher's exact test for biased strand distribution p-value = 7.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

AGAATCAGCAGAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAACA  >  CP000730/2455553‑2455829

aGAATCAGCAGAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGa                                                                                                                                           <  1:628580/141‑1 (MQ=255)

 gAATCAGCAGAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGaa                                                                                                                                          >  1:770095/1‑141 (MQ=255)

    tCAGCAGAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGaaaaa                                                                                                                                       >  2:463990/1‑141 (MQ=255)

      agcagAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACa                                                                                                                                     >  2:380885/1‑141 (MQ=255)

      agcagAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACa                                                                                                                                     >  2:278187/1‑141 (MQ=255)

       gcagAATCAGTTAAACAAGCACCACAAAGAGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAaaaaaatta                                                                                                                                    >  2:77608/1‑138 (MQ=255)

        cagAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACata                                                                                                                                   >  1:28513/1‑141 (MQ=255)

        cagAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACata                                                                                                                                   >  1:758896/1‑141 (MQ=255)

           aaTCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGCAAAATAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCg                                                                                                                                <  1:410182/141‑1 (MQ=255)

           aaTCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCg                                                                                                                                <  2:38816/141‑1 (MQ=255)

            aTCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAAGCAATAAAAGAACCTGCGACATATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACCTATCGa                                                                                                                               <  2:18643/141‑1 (MQ=255)

            aTCAGTTAAACAAGCACCACAAAGGGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGa                                                                                                                               <  1:625581/141‑1 (MQ=255)

               aGTTAAACAAGCACCACAAAGTGACGATTTTACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGa‑aaaa                                                                                                                          >  2:597156/1‑141 (MQ=255)

                gTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGa‑aaaa                                                                                                                          >  2:117673/1‑141 (MQ=255)

                gTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGa‑aaaa                                                                                                                          >  1:423358/1‑141 (MQ=255)

                  tAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAAc                                                                                                                        <  2:456755/141‑1 (MQ=255)

                   aaaCAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACt                                                                                                                       >  1:398087/1‑141 (MQ=255)

                      cAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTgaa                                                                                                                    >  2:43005/1‑141 (MQ=255)

                      cAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGA‑AAAAACTgaag                                                                                                                   >  2:274275/1‑141 (MQ=255)

                       aaGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTgaag                                                                                                                   >  2:596370/1‑141 (MQ=255)

                        cgCAACACTAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTgcaga                                                                                                                  <  2:424758/140‑1 (MQ=255)

                        aGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTgaaga                                                                                                                  <  2:171070/141‑1 (MQ=255)

                          caccacAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTGAAGAAc                                                                                                                >  1:54569/1‑141 (MQ=255)

                          caccacAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTGAAGAAc                                                                                                                >  1:65527/1‑141 (MQ=255)

                            ccacAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGA‑AAAAACTGAAGAACAAt                                                                                                             >  2:700424/1‑141 (MQ=255)

                               caAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCt                                                                                                           >  2:637434/1‑141 (MQ=255)

                                aaaGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTgaagattgttctt                                                                                                          <  2:221297/141‑9 (MQ=255)

                                aaaGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCtt                                                                                                          >  2:531333/1‑141 (MQ=255)

                                     gACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGAt                                                                                                     <  1:302929/141‑1 (MQ=255)

                                      aCGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATa                                                                                                    >  1:153612/1‑141 (MQ=255)

                                          tttAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATaaaaa                                                                                                <  1:292944/141‑1 (MQ=255)

                                            tAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACa                                                                                              <  1:560252/141‑1 (MQ=255)

                                               cAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAaat                                                                                           <  1:265376/141‑1 (MQ=255)

                                                aaaCGATAAAAATGAAGATGAAAAAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAAAAAAAAAA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAaata                                                                                          <  2:151648/141‑1 (MQ=255)

                                                     aTTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAAc                                                                                     <  2:287046/141‑1 (MQ=255)

                                                      ttCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGA‑AAAACCTGAAGAACAATCTTCAGATAAAAACAAATAATAACaa                                                                                   <  1:415383/141‑1 (MQ=255)

                                                       tCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACaa                                                                                   >  2:556921/1‑141 (MQ=255)

                                                       tCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACaa                                                                                   <  2:335243/141‑1 (MQ=255)

                                                           aTGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAgcg                                                                               >  2:719823/1‑141 (MQ=255)

                                                                    aaaTAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGt                                                                      >  1:775435/1‑141 (MQ=255)

                                                                    aaaTAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGt                                                                      >  1:775436/1‑141 (MQ=255)

                                                                               gcATTAAAAGAGCCGGCGACTTATAAAGAAGACAGACGTTCAGCAGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaaaa                                                           <  2:657957/140‑1 (MQ=255)

                                                                                       aGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGatgatg                                                   >  2:37541/1‑141 (MQ=255)

                                                                                            cTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGc                                              <  1:767633/141‑1 (MQ=255)

                                                                                            cTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCa                                             <  1:202059/141‑1 (MQ=255)

                                                                                             tGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCa                                             >  1:453255/1‑141 (MQ=255)

                                                                                             tGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCa                                             <  2:337664/141‑1 (MQ=255)

                                                                                              gCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCaa                                            <  1:278187/141‑1 (MQ=255)

                                                                                              gCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCaa                                            >  2:572628/1‑141 (MQ=255)

                                                                                                  cTTATAAAGAAGACAGACGTTCATCATTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgcgc                                        <  2:623813/141‑1 (MQ=255)

                                                                                                     ataAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCttt                                     >  1:518749/1‑141 (MQ=255)

                                                                                                      taAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCtttt                                    <  2:40414/141‑1 (MQ=255)

                                                                                                         agaagaCAGACGTTCATCAGTTGTAATTGATGCAAAAAAA‑TTATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctat                                >  1:131243/1‑141 (MQ=255)

                                                                                                            agacagacGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctatac                              <  1:591689/141‑1 (MQ=255)

                                                                                                            agacagacGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAAAAAAAACAAAGCGCAAGCTAGTACCTCTTAAAAAACTGATGCGAGCAAGCGCTATTctctaa                              >  2:745094/1‑140 (MQ=255)

                                                                                                              acagacGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctatactat                           >  1:318768/1‑141 (MQ=255)

                                                                                                                agacGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctatactata                          >  2:598204/1‑141 (MQ=255)

                                                                                                                 gacGTTCATCAGTTGTAATTGATGCA‑AAAAAATTATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACtatata                        >  2:223375/1‑141 (MQ=255)

                                                                                                                 gacGGTCATCAGGTGTAAGTGATGCAGATAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACtatat                         <  2:68395/141‑1 (MQ=255)

                                                                                                                   cGTTCATCAATTGTCATTACTCAAGAAAACCATATAGACAAAAACTCAAGAAAAACCTTCACATCACCACAAACCCAAACAACGCGCTTGCTAGTACCTCTTACAAAGATGATGCTAGCAAGCGCTTTTCTATACtatata                        <  1:589287/141‑1 (MQ=255)

                                                                                                                       catcaGTTGTAATTGATGCAGAAAAACATATCGA‑CAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATattatt                   >  2:77973/1‑141 (MQ=255)

                                                                                                                       catcaGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATattatt                   >  2:14260/1‑141 (MQ=255)

                                                                                                                       catcaGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATattatt                   <  2:38565/141‑1 (MQ=255)

                                                                                                                       catcaGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATattatt                   <  1:277652/141‑1 (MQ=255)

                                                                                                                        atcaGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTAttt                  <  1:463990/141‑1 (MQ=255)

                                                                                                                        atcaGTTGTAATTAATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTAttt                  <  2:507987/141‑1 (MQ=255)

                                                                                                                         tcaGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTAtttt                 >  1:89812/1‑141 (MQ=255)

                                                                                                                               gTAATTGATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTaaa           >  1:145618/1‑141 (MQ=255)

                                                                                                                                  aTTGATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCGAAAAtt        >  2:749365/1‑141 (MQ=255)

                                                                                                                                   ttGATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAAttt       <  2:573995/141‑1 (MQ=255)

                                                                                                                                    tGATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAAtttt      >  1:251092/1‑141 (MQ=255)

                                                                                                                                     gATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTa     >  1:368034/1‑141 (MQ=255)

                                                                                                                                     gATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTa     >  2:645558/1‑141 (MQ=255)

                                                                                                                                     gATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTa     >  2:467025/1‑141 (MQ=255)

                                                                                                                                     gATGCA‑AAAAAATTATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTaa    <  1:274275/141‑1 (MQ=255)

                                                                                                                                      aTGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTaa    >  2:473440/1‑141 (MQ=255)

                                                                                                                                        gCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAaca  <  2:725448/141‑1 (MQ=255)

                                                                                                                                        gCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAaca  <  2:31002/141‑1 (MQ=255)

AGAATCAGCAGAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGA‑AAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAACA  >  CP000730/2455553‑2455829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A