Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F59 I1 R1	27	28.6	614024	91.3%	560603	148.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	1,977,348	G→C	L368V (CTA→GTA)	sbcD ←	DNA repair exonuclease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,977,348	0	G	C	100.0%	95.9 / NA	27	L368V (CTA→GTA)	sbcD	DNA repair exonuclease
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (12/15); total (12/15)

TTCAATTTTACGTTGAACAAATTGACCGTAACCATAAATTTCAAGTGATTTAATTATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTAGGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAGTTTGATCGACTAATAATTCCGCTGAAAATTCATTAACTAAAGGTGACTCATCATTTTGTGCATATTGTATTTTTAACTCATC  >  CP000730/1977204‑1977486

ttCAATTTTACGTTGAACAAATTGACCGTAACCATAAATTTCAAGTGATTTAATTATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGaa                                                                                                                                         <  2:4733/148‑1 (MQ=255)

 ttaaTTTTACGTTGAACAGATTGACCGTATCCATAAATTTCAAGTGATTTAATTATCATTTTGTTCACCTCTCATTTCAGCTTTTCGTATTTCTTCAGCACGATTAACTAATGCTGCATGGTCGAATGTTCCATAATCGTCTACGAAc                                                                                                                                        <  2:45740/146‑1 (MQ=255)

 tCAATTTTACGTTGAACATATTGACCGTAACCATAAATTTCAAGTGATTTAATTATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAAc                                                                                                                                        <  1:233800/148‑1 (MQ=255)

 tCAATTTTACGTTGAACAAATTGACCGTAACCATAAATTTCAAGTGATTTAATTATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAAc                                                                                                                                        <  1:204668/148‑1 (MQ=255)

 tCAATTTTACGTTGAACAAATTGACCGTAACCATAAATTTCAAGTGATTTAATTATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAAc                                                                                                                                        <  1:172131/148‑1 (MQ=255)

  cAATTTTACGTTGAACAAATTGACCGTAACCATAAATTTCAAGTGATTTAATTATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACt                                                                                                                                       <  2:174540/148‑1 (MQ=255)

    aTTTTACGTTGAACAAATTGACCGTAACCATAAATTTCAAGTGATTTAATTATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTa                                                                                                                                     >  2:38926/1‑148 (MQ=255)

          cGTTGAACAAATTGACCGTAACCATACATTTCAAGTGATTTCATTATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGcc                                                                                                                               <  1:124057/148‑1 (MQ=255)

               aaCAAATTGACCGTAACCATAAATTTCAAGTGATTTAATTATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTgg                                                                                                                          <  1:218222/148‑1 (MQ=255)

                 cAAATTGACCGTAACCATAAATTTCAAGTGATTTAATTATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGAt                                                                                                                        <  2:142648/148‑1 (MQ=255)

                            tAACCATAAATTTCAAGTGATTTAATTATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGATTTAAATATaaa                                                                                                             >  2:172634/1‑148 (MQ=255)

                             aaCCATAAATTTCAAGTGATTTAATTATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAAt                                                                                                            <  2:112748/148‑1 (MQ=255)

                                               aTTTAATTATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCaa                                                                                          >  1:140863/1‑148 (MQ=255)

                                                     ttATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAg                                                                                    >  2:59987/1‑148 (MQ=255)

                                                        tCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAGttt                                                                                 >  1:213861/1‑148 (MQ=255)

                                                        tCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAGttt                                                                                 >  1:217487/1‑148 (MQ=255)

                                                        tCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAGttt                                                                                 >  1:213881/1‑148 (MQ=255)

                                                                 tCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAGTTTGATCGACta                                                                        >  1:10214/1‑148 (MQ=255)

                                                                 tCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAGTTTGATCGACta                                                                        >  1:193704/1‑148 (MQ=255)

                                                                                                     cGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAGTTTGATCGACTAATAATTCCGCTGAACATTCAGTAACTAAAGGTGACt                                    >  2:273678/1‑148 (MQ=255)

                                                                                                     cGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAGTTTGATCGACTAATAATTCCGCTGAAAATTCATTAACTAAAGGTGACt                                    >  2:189220/1‑148 (MQ=255)

                                                                                                       aTTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAGTTTGATCGACTAATAATTCCGCTGAAAATTCATTAACTAAAGGTGACtca                                  <  2:117904/148‑1 (MQ=255)

                                                                                                            cTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAGTTTGATCGACTAATAATTCCGCTGAAAATTCATTAACTAAAGGTGACTCATCAtt                             <  1:102593/148‑1 (MQ=255)

                                                                                                                         gTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAGTTTGATCGACTAATAATTCCGCTGAAAATTCATTAACTAAAGGTGACTCATCATTTTGTGCATATTGt                <  1:109501/148‑1 (MQ=255)

                                                                                                                                       aaTCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAGTTTGATCGACTAATAATTCCGCTGAAAATTCATTAACTAAAGGTGACTCATCATTTTGTGCATATTGTATTTTTAACtcatc  <  2:285517/148‑1 (MQ=255)

                                                                                                                                       aaTCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAGTTTGATCGACTAATAATTCCGCTGAAAATTCATTAACTAAAGGTGACTCATCATTTTGTGCATATTGTATTTTTAACtcatc  >  2:192172/1‑148 (MQ=255)

                                                                                                                                       aaTCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGAAAAAAAAAAAAAAAAAACAGTTTGATCGACTAATAATTCCGCTGAAAATTCATTAACTAAAGGTGACTCATCATTTTGTGCATATTGTATTTTTAACtcatc  <  2:207656/148‑1 (MQ=255)

TTCAATTTTACGTTGAACAAATTGACCGTAACCATAAATTTCAAGTGATTTAATTATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTAGGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAGTTTGATCGACTAATAATTCCGCTGAAAATTCATTAACTAAAGGTGACTCATCATTTTGTGCATATTGTATTTTTAACTCATC  >  CP000730/1977204‑1977486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A