Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F169 I1 R1	59	38.6	831760	90.8%	755238	148.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	505,514	C→T	A469V (GCG→GTG)	treP →	PTS family trehalose (tre) porter component IIBC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	505,514	0	C	T	100.0%	120.3 / NA	38	A469V (GCG→GTG)	treP	PTS family trehalose (tre) porter component IIBC
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (17/21); total (17/21)

GTTGGTGGCGTGCCAGCATTCATTTCAATTCAAAAAGAATTTTGGCCAGTATATCTTATTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGCGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAAGAAATAGATTGGAGAAAATCCGTTGTATATCAAATTT  >  CP000730/505384‑505645

gttggtgGCGTGCCAGCATTCATTTCAATTCAAAAAGAATTTTGGCCAGTATATCTTATTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAg                                                                                                                    >  2:41326/1‑148 (MQ=255)

gttggtgGCGTGCCAGCATTCATTTCAATTCAAAAAGAATTTTGGCCAGTATATCTTATTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAg                                                                                                                    >  2:216943/1‑148 (MQ=255)

gttggtgGCGTGCCAGCATTCATTTCAATTCAAAAAGAATTTTGGCCAGTATATCTTATTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAg                                                                                                                    >  1:286073/1‑148 (MQ=255)

   ggtggCGTGCCAGCATTCATTTCAATTCAAAAAGAATTTTGGCCAGTATATCTTATTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGAtt                                                                                                                 >  1:261338/1‑148 (MQ=255)

     tggCGTGCCAGCATTCATTTCAATTCAAAAAGAATTTTGGCCAGTATATCTTATTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATtaa                                                                                                               <  1:72680/148‑1 (MQ=255)

        cGTGCCAGCATTCATTTCAATTCAAAAAGAATTTTGGCCAGTATATCTTATTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATtaataa                                                                                                            <  2:97144/148‑1 (MQ=255)

        cGTGCCAGCATTCATTTCAATTCAAAAAGAATTTTGGCCAGTATATCTTATTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATtaataa                                                                                                            <  1:151949/148‑1 (MQ=255)

        cGTGCCAGCATTCATTTCAATTCAAAAAGAATTTTGGCCAGTATATCTTATTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATtaataa                                                                                                            <  1:373108/148‑1 (MQ=255)

        cGTGCCAGCATTCATTTCAATTCAAAAAGAATTTTGGCCAGTATATCTTATTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATtaataa                                                                                                            <  2:43413/148‑1 (MQ=255)

        cGTGCCAGCATTCATTTCAATTCAAAAAGAATTTTGGCCAGTATATCTTATTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATtaataa                                                                                                            <  2:144172/148‑1 (MQ=255)

                        tcaattcaaAAAGAATTTTGGCCAGTATATCTTATTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGcgt                                                                                            >  1:97088/1‑148 (MQ=255)

                                 aaaGAATTTTGGCCAGTATATCTTATTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTAttt                                                                                   <  1:174374/148‑1 (MQ=255)

                                  aaGAATTTTGGCCAGTATATCTTATTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTg                                                                                  <  1:327567/148‑1 (MQ=255)

                                           ggCCAGTATATCTTATTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCtt                                                                         >  2:341950/1‑148 (MQ=255)

                                           ggCCAGTATATCTTATTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCtt                                                                         <  2:251605/148‑1 (MQ=255)

                                            gCCAGTATATGTTATTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAAAAAAAAAGGGGCGTTCGTTATTTGGACGTCCttt                                                                        <  2:29954/148‑1 (MQ=255)

                                                         aTTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAg                                                           <  2:28771/148‑1 (MQ=255)

                                                                  gctattgctattGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAAtt                                                  <  1:284144/148‑1 (MQ=255)

                                                                    tattgctattgTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATtgt                                                >  1:198204/1‑148 (MQ=255)

                                                                       tgctattgTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATtgtgtg                                             <  2:261338/148‑1 (MQ=255)

                                                                               ttgtACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCgaaaga                                     <  1:370301/148‑1 (MQ=255)

                                                                                   aCCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAAGAAATa                                 >  2:260335/1‑148 (MQ=255)

                                                                                    ccATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAAGAAATAg                                >  2:317696/1‑148 (MQ=255)

                                                                                             cTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAAGAAATAGATTGGAGaa                       >  1:40769/1‑148 (MQ=255)

                                                                                                    ttGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAAGAAATAGATTGGAGAAAATCCGt                <  2:316181/148‑1 (MQ=255)

                                                                                                    ttGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAAGAAATAGATTGGAGAAAATCCGt                <  1:79811/148‑1 (MQ=255)

                                                                                                    ttGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAAGAAATAGATTGGAGAAAATCCGt                <  1:230280/148‑1 (MQ=255)

                                                                                                      gtgATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAAGAAATAGATTGGAGAAAATCCGTTg              <  1:2833/148‑1 (MQ=255)

                                                                                                      gtgATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAAGAAATAGATTGGAGAAAATCCGTTg              <  1:67463/148‑1 (MQ=255)

                                                                                                        gATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAAGAAATAGATTGGAGAAAATCCGTTGta            >  2:162577/1‑148 (MQ=255)

                                                                                                         aTGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAAGAAATAGATTGGAGAAAATCCGTTGtat           >  1:233988/1‑148 (MQ=255)

                                                                                                         aTGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAAGAAATAGATTGGAGAAAATCCGTTGtat           >  2:192023/1‑148 (MQ=255)

                                                                                                         aTGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAAGAAATAGATTGGAGAAAATCCGTTGtat           >  2:41557/1‑148 (MQ=255)

                                                                                                             ctcAGTTTAGGAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAAGAAATAGATTGGAGAAAATACGTTCTAAATCa       >  2:327931/1‑148 (MQ=255)

                                                                                                                aTTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATCAAATAAGAAAGGGGCGTTCGTTATTTGGACGCCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAAGAAATAGATTGGAGAAAATCCGTTGTATATCAAAt    >  1:357729/1‑148 (MQ=255)

                                                                                                                aTTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAAGAAATAGATTGGAGAAAATCCGTTGTATATCAAAt    >  1:236995/1‑148 (MQ=255)

                                                                                                                  tttAGTAAACAAAAAGTGAAAGAAATTGTTGAATATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAATAAATAGATTGGAGAAAATCCGTTGTATATCAAAttt  <  2:229736/148‑1 (MQ=255)

                                                                                                                  tttAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAAGAAATAGATTGGAGAAAATCCGTTGTATATCAAAttt  <  1:391536/148‑1 (MQ=255)

GTTGGTGGCGTGCCAGCATTCATTTCAATTCAAAAAGAATTTTGGCCAGTATATCTTATTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGCGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAAGAAATAGATTGGAGAAAATCCGTTGTATATCAAATTT  >  CP000730/505384‑505645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A