Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F169 I1 R1	59	38.6	831760	90.8%	755238	148.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,018,612	G→A	R103* (CGA→TGA)	mgt ←	transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,018,612	0	G	A	100.0%	87.9 / NA	28	R103* (CGA→TGA)	mgt	transglycosylase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (13/15); total (13/15)

GAACGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAAT  >  CP000730/2018473‑2018754

gAACGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCtt                                                                                                                                        >  1:70963/1‑148 (MQ=255)

             cATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATaaa                                                                                                                           <  2:165618/148‑1 (MQ=255)

                       atTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGCTTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTa                                                                                                                 >  2:387675/1‑148 (MQ=255)

                       atTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTa                                                                                                                 >  2:116463/1‑148 (MQ=255)

                             gACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATAc                                                                                                           <  2:28842/148‑1 (MQ=255)

                             gACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATAc                                                                                                           <  1:249682/148‑1 (MQ=255)

                               cAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATActc                                                                                                         >  2:229536/1‑148 (MQ=255)

                                         tgtAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTa                                                                                               <  2:298330/148‑1 (MQ=255)

                                         tgtAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTa                                                                                               <  2:367589/148‑1 (MQ=255)

                                                         ccTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAac                                                                               >  2:188681/1‑148 (MQ=255)

                                                         ccTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAac                                                                               >  2:389325/1‑148 (MQ=255)

                                                           ttGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGCACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAacta                                                                             >  1:300561/1‑148 (MQ=255)

                                                                caTCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACtttt                                                                        >  1:189712/1‑148 (MQ=255)

                                                                    tctGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTAttt                                                                    <  1:8328/148‑1 (MQ=255)

                                                                    tctGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTAttt                                                                    <  1:11719/148‑1 (MQ=255)

                                                                    tctGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTAttt                                                                    <  1:266810/148‑1 (MQ=255)

                                                                                cGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCtt                                                        <  2:9127/148‑1 (MQ=255)

                                                                                cGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCtt                                                        >  2:346156/1‑148 (MQ=255)

                                                                                                  aGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATcg                                      <  1:229536/148‑1 (MQ=255)

                                                                                                  aGTTGTACCTTTCAAATCAAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATcg                                      <  1:316390/148‑1 (MQ=255)

                                                                                                    ttGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTTATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATcgcg                                    <  1:189540/148‑1 (MQ=255)

                                                                                                                aaTCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATaaaaa                        <  1:235056/148‑1 (MQ=255)

                                                                                                                 aTCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATaaaaaa                       >  2:217701/1‑148 (MQ=255)

                                                                                                                          atgatgATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATa              <  1:81966/148‑1 (MQ=255)

                                                                                                                               gatTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAAt         >  1:262041/1‑148 (MQ=255)

                                                                                                                                atTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATa        <  1:372404/148‑1 (MQ=255)

                                                                                                                                 ttGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATaa       >  1:342315/1‑148 (MQ=255)

                                                                                                                                      gAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAAt  >  2:258506/1‑148 (MQ=255)

GAACGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAAACAAT  >  CP000730/2018473‑2018754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A