Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F104 I1 R1	32	52.7	1196080	90.6%	1083648	140.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,018,612	G→A	R103* (CGA→TGA)	mgt ←	transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,018,612	0	G	A	100.0%	168.9 / NA	53	R103* (CGA→TGA)	mgt	transglycosylase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (30/23); total (30/23)

CGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAA  >  CP000730/2018476‑2018749

cGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCa                                                                                                                                       >  1:904034/1‑141 (MQ=255)

agaccataagcATAAAAAAAATTTTGCACAACCTGCTGTCTAATGGTCCTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCa                                                                                                                                       <  1:410555/131‑1 (MQ=255)

 gATCATTATCATAAAAATAATTTATGACAACTGGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAAtcattcat                                                                                                                                      >  1:286950/1‑141 (MQ=255)

       tATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCa                                                                                                                                >  1:980590/1‑141 (MQ=255)

              aaaaTAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAAt                                                                                                                         <  1:401184/141‑1 (MQ=255)

              aaaaTAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAAt                                                                                                                         <  1:1157767/141‑1 (MQ=255)

                aataatTTTTGACAACGTGTTGTGTAATGGTACTACCACCTTGTACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATaa                                                                                                                       <  1:377011/141‑1 (MQ=255)

                 ataatTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATaaa                                                                                                                      <  1:1094647/141‑1 (MQ=255)

                   aatTTTTGACAACTTGTTGTGTAATGGTACTAGCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGCTGTACCGTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAgg                                                                                                                    <  1:338748/141‑1 (MQ=255)

                   aatTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAgg                                                                                                                    >  1:850069/1‑141 (MQ=255)

                   aatTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAgg                                                                                                                    >  1:755483/1‑141 (MQ=255)

                   aatTTTTGACAACTTGTTGTATAGTGGTCCTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGTTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAgg                                                                                                                    <  1:828463/141‑1 (MQ=255)

                     tttttGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATGTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGGATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCa                                                                                                                  >  1:820030/1‑141 (MQ=255)

                     tttttGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCa                                                                                                                  >  1:204778/1‑141 (MQ=255)

                       tttGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCAcc                                                                                                                <  1:146650/141‑1 (MQ=255)

                              ccctgtcgtGTAATGCTACTACCCCCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAGGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACa                                                                                                         <  1:10514/138‑1 (MQ=255)

                                ttgttgTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATa                                                                                                       <  1:882549/141‑1 (MQ=255)

                                    tgtgTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAACATAAAGCGCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATCctct                                                                                                   >  1:628948/1‑141 (MQ=255)

                                          aTGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGCAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAGCATACTCTGGCAtt                                                                                             >  1:853224/1‑140 (MQ=255)

                                                   ccaccTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCt                                                                                    >  1:227909/1‑141 (MQ=255)

                                                      ccTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTGGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCGTGTTATCAGCTggc                                                                                 >  1:249519/1‑139 (MQ=255)

                                                      ccTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCCCCTTTAACATACACTGGCATGTTGTCAGcgacc                                                                                 >  1:197411/1‑137 (MQ=255)

                                                      ccTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGac                                                                                 >  1:913129/1‑141 (MQ=255)

                                                      ccTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAAGCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGATTGTTATCAGCTGac                                                                                 >  1:348808/1‑141 (MQ=255)

                                                      ccTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTCCCAAATTGAATCCATGATGATGGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGac                                                                                 >  1:226959/1‑141 (MQ=255)

                                                          gCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCGGCTGACACaa                                                                             >  1:315272/1‑141 (MQ=255)

                                                          gCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACaa                                                                             >  1:642739/1‑141 (MQ=255)

                                                          gCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACaa                                                                             >  1:806483/1‑141 (MQ=255)

                                                               tctcTGTCGCTAATCGATGCAAATAAAGCTCTAGTTGTGCCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATGGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAacta                                                                        >  1:668953/1‑141 (MQ=255)

                                                                       gCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTAtt                                                                >  1:791644/1‑141 (MQ=255)

                                                                       gCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTAtt                                                                >  1:791520/1‑141 (MQ=255)

                                                                        cTACTCGTTGAAAATAAAGCTCTATTTGCACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTAttt                                                               <  1:219453/141‑1 (MQ=255)

                                                                             cGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTCTCAGCTGACACAAAACTACTTTTATTTTCAAt                                                          >  1:825070/1‑141 (MQ=255)

                                                                                    aaTAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTAAATTTTTCtt                                                   >  1:239360/1‑141 (MQ=255)

                                                                                        aaGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGtt                                               <  1:1089854/141‑1 (MQ=255)

                                                                                        aaGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTACATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGtt                                               <  1:319349/141‑1 (MQ=255)

                                                                                               aGTTGTACCTTTCAAATCGAATCCCTGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCcac                                        <  1:497116/141‑1 (MQ=255)

                                                                                                   gTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACAttt                                    >  1:641196/1‑140 (MQ=255)

                                                                                                   gTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTa                                    >  1:735436/1‑141 (MQ=255)

                                                                                                   gTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTa                                    >  1:128454/1‑141 (MQ=255)

                                                                                                    tACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTAt                                   <  1:906484/141‑1 (MQ=255)

                                                                                                     aCCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATc                                  <  1:843724/141‑1 (MQ=255)

                                                                                                      cccttCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATcg                                 <  1:471331/138‑1 (MQ=255)

                                                                                                            aaaTCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCCCATTATcgtgcgtt                           >  1:463955/1‑135 (MQ=255)

                                                                                                            aaaTCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTa                           >  1:74906/1‑141 (MQ=255)

                                                                                                            aaaTCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTa                           >  1:464061/1‑141 (MQ=255)

                                                                                                              aTCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGa                         <  1:907200/141‑1 (MQ=255)

                                                                                                              aTCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGa                         <  1:715866/141‑1 (MQ=255)

                                                                                                               tCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCCCCTGTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACACTATCGCGTGTAGAt                        <  1:624917/141‑1 (MQ=255)

                                                                                                                 gAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATaa                      >  1:229979/1‑141 (MQ=255)

                                                                                                                      cATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAt                 <  1:1046173/141‑1 (MQ=255)

                                                                                                                       atgatgATTGTAGAATCATTCATCTTCCATTGAAATAGAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATa                <  1:1110919/141‑1 (MQ=255)

                                                                                                                       atgatgATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATa                >  1:1179237/1‑141 (MQ=255)

                                                                                                                                     aTCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATaa  <  1:810356/141‑1 (MQ=255)

CGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATACCAATAA  >  CP000730/2018476‑2018749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A