breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 36,378 C→T L58L (TTG→TTA tpn ← IS431mec transposase
RA 39,378 C→T D586N (GAT→AAT)  mecA ← penicillin binding protein 2 prime
RA 76,969 G→A intergenic (+1027/‑107) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 129,796 G→C intergenic (‑173/+212) spa ← / ← sarH1 immunoglobulin G binding protein A/staphylococcal accessory regulator H1
RA 195,286 T→A intergenic (‑97/‑82) USA300HOU_0183 ← / → USA300HOU_0184 hypothetical protein/hypothetical protein
RA 396,540 G→A A208T (GCA→ACA)  USA300HOU_0364 → possible lipoprotein
RA 536,758 C→T P202L (CCA→CTA)  rplY → ribosomal protein L25
RA 607,884 G→A R209* (CGA→TGA)  dck ← deoxynucleoside kinase
RA 629,681 C→A L84I (CTA→ATA)  hxlA → possible 3‑hexulose‑6‑phosphate synthase
RA 665,392 T→G L79* (TTA→TGA)  iolS → possible acetytransferase
RA 707,910 A→G intergenic (‑1/‑121) mntB ← / → sirR ABC superfamily ATP binding cassette transporter, ABC protein/possible metal‑dependent transcriptional regulator
RA 1,063,805 +T coding (684/1218 nt) brpA ← LytR family transcriptional regulator
RA 1,125,066 G→C E729Q (GAG→CAG)  pycA → pyruvate carboxylase
RA 1,233,504 T→A V470D (GTT→GAT)  prkC → non‑specific serine/threonine protein kinase
RA 1,298,108 T→A intergenic (+4/‑232) pnp → / → rnjB polyribonucleotide nucleotidyltransferase/beta‑lactamase
RA 1,416,458 A→T I216N (ATT→AAT)  oppD ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
RA 1,440,085 G→T intergenic (‑45/‑891) cspA ← / → xpaC cold shock protein CspA/5‑bromo‑4‑chloroindolyl phosphate hydrolysis protein XpaC
RA 1,499,376 C→A G883V (GGT→GTT)  ebh ← extracellular matrix binding protein
RA 1,513,930 A→T L413F (TTA→TTT USA300HOU_1379 → hypothetical membrane protein
RA 1,624,673 T→A K192I (AAA→ATA)  malA ← alpha‑glucosidase
RA 1,646,189 G→C A155G (GCA→GGA)  pepP ← M24B family Xaa‑Pro dipeptidase
RA 1,744,362 G→A H104Y (CAC→TAC)  apt ← adenine phosphoribosyltransferase
RA 1,761,889 C→A D388Y (GAT→TAT)  folC ← tetrahydrofolate synthase
RA 1,851,260 C→A A115S (GCG→TCG)  acsA1 ← acetate‑‑CoA ligase
RA 1,963,768 C→G V365L (GTA→CTA)  hemG ← protoporphyrinogen oxidase
RA 2,010,363 C→T C146Y (TGT→TAT)  ygaD ← ABC superfamily ATP binding cassette transporter, membrane protein
RA 2,018,612 G→A R103* (CGA→TGA)  mgt ← transglycosylase
RA 2,279,705 G→A intergenic (+1373/+6659) mtlD → / ← glmM mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase
RA 2,352,815 G→A A92V (GCA→GTA)  rpsI ← ribosomal protein S9
RA 2,454,626 A→T L48L (CTA→CTT USA300HOU_2315 → hypothetical membrane protein
RA 2,532,298 A→T T15T (ACA→ACT murN → FemAB family peptidoglycan biosynthesis protein
RA 2,543,088 G→A R283K (AGA→AAA)  hlgA → gamma‑hemolysin component A

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 512620 =55 (1.050)38 (1.020)
+40 bp
27/200 0.2 49.2% intergenic (+688/‑173) recR/rrs1 recombination protein RecR/16S ribosomal RNA
?CP000730 1998278 = NA (NA)intergenic (‑6/+5) USA300HOU_t0050/rrf4 tRNA‑Val/5S ribosomal RNA