Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F169 I1 R1	59	38.6	831760	90.8%	755238	148.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,679,462	(A)_8→7	intergenic (+334/‑55)	USA300HOU_2535 → / → cbpD	hypothetical protein/possible secretory antigen

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,679,455	0	A	.	96.9%	140.6 / ‑1.5	32	intergenic (+327/‑62)	USA300HOU_2535/cbpD	hypothetical protein/possible secretory antigen
Reads supporting (aligned to +/- strand): ref base A (0/1); new base . (12/19); total (12/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

TGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGTTTATTAATTGCTTTTGCAGTACTTTTCTCAGCAGATTTCA  >  CP000730/2679308‑2679583

tGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTa                                                                                                                                  >  2:335978/1‑148 (MQ=255)

  aCATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTaaa                                                                                                                                >  2:326692/1‑148 (MQ=255)

  aCATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTaaa                                                                                                                                >  2:326705/1‑148 (MQ=255)

        ttGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTaaaaaaatt                                                                                                                          >  1:59593/1‑146 (MQ=255)

         tGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTaaaaaaattt                                                                                                                         <  2:271971/148‑4 (MQ=255)

            aTCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTTTTTTTTTACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCT‑AAAAAAATTTGtt                                                                                                                     >  2:386193/1‑148 (MQ=255)

                 tATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTATTTTTTTTTTCTTTATATTATAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCT‑AAAATAATTTGTTATCAt                                                                                                                >  2:179893/1‑148 (MQ=255)

                       aattaattGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCT‑AAAAAAATTTGTTATCATAATTaa                                                                                                          >  1:128654/1‑148 (MQ=255)

                               gTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCT‑AAAAAAATTTGTTATCATAATTAACGTTGAAt                                                                                                  >  2:71006/1‑148 (MQ=255)

                                 tAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCT‑AAAAAAATTTGTTATCATAATTAACGTTGAATaa                                                                                                <  2:62268/148‑1 (MQ=255)

                                 tAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCT‑AAAAAAATTTGTTATCATAATTAACGTTGAATaa                                                                                                >  1:375689/1‑148 (MQ=255)

                                       cATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTCCTTTTATTGTTGATTGCTTACATTGTTTTCT‑AAAAAAATTTGTTATCATAATTAACGTTGAATAAAGaaaa                                                                                          >  2:394421/1‑148 (MQ=255)

                                          gAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCT‑AAAAAAATTTGTTATCATAATTAACGTTGAATAAAGaaaaaaa                                                                                       <  1:273362/148‑1 (MQ=255)

                                            aTTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCT‑AAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAAtt                                                                                     >  1:326527/1‑148 (MQ=255)

                                                  aCATTTTATGAAAATATCCATTTTTATTACAATTAAACAATTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCT‑AAAAAAATTTGTTATCATAATTAACATTAAAAAAAAAAAAAAATTAACTTg                                                                               <  2:188415/148‑1 (MQ=255)

                                                  aCATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCT‑AAAAAGATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTg                                                                               <  1:349806/148‑1 (MQ=255)

                                                  aCATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCT‑AAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTg                                                                               <  2:45523/148‑1 (MQ=255)

                                                                aaTCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCT‑AAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATgg                                                                 <  2:130017/148‑1 (MQ=255)

                                                                aaTCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCT‑AAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATgg                                                                 <  2:1961/148‑1 (MQ=255)

                                                                aaTCCATTTTTATTACAAGTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCT‑AAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATgg                                                                 <  1:335509/148‑1 (MQ=255)

                                                                aaTCCATTTTTAATACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCT‑AAAAAAATTTGTTATCTTAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATgg                                                                 <  2:58198/148‑1 (MQ=255)

                                                                   ccATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCT‑AAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAat                                                              <  2:95117/148‑1 (MQ=255)

                                                                   ccATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTAT‑AAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAat                                                              <  2:274905/148‑1 (MQ=255)

                                                                         ttattaCAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCT‑AAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATaaaa                                                        <  1:13181/148‑1 (MQ=255)

                                                                              aCAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCT‑AAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATa                                                   <  1:324668/148‑1 (MQ=255)

                                                                                         cTTTATATTACAAATTCATTACAGATACTACAAATATTGATTGCTTACAATGTATTCTAAAAAAAATTT‑TTATCATTATTAAAATAAAAAAAAAAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGttt                                        <  2:198157/148‑1 (MQ=255)

                                                                                           ttATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCT‑AAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGTttat                                      >  1:397706/1‑148 (MQ=255)

                                                                                                tGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCT‑AAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGTTTATTAAtt                                 <  1:286766/148‑1 (MQ=255)

                                                                                                   cAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCT‑AAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGTTTATTAATTGCt                              >  1:319856/1‑148 (MQ=255)

                                                                                                            tACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCT‑AAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGTTTATTAATTGCTTTTGCAGTa                     <  1:261903/148‑1 (MQ=255)

                                                                                                             aCAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCT‑AAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGTTTATTAATTGCTTTTGCAGTAc                    <  1:168446/148‑1 (MQ=255)

                                                                                                                 ttACTTTTATTGTTGATTGCTTACATTGTTTTCT‑AAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACGTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGTTTATTAATTGCTTTCGCAGTACtttt                >  2:48860/1‑148 (MQ=255)

                                                                                                                           tgttgATTGCTTACATTGTTTTCT‑AAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGTTTATTAATTGCTTTTGCAGTTATTTTCTCAGCAGAt      >  1:127797/1‑148 (MQ=255)

                                                                                                                            gttgATTGCTTACATTGTTTTCT‑AAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGTTTATTAATTGCTTTTGCAGTACTTTTCTCAGCAGAtt     >  1:5270/1‑148 (MQ=255)

                                                                                                                              tgATTGCTTACATTGTTTTCT‑AAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGTTTATTAATTGCTTTTGCAGTACTTTTCTCAGCAGATTTc   <  2:170073/148‑1 (MQ=255)

                                                                                                                              tgATTGCTTACATTAATTTCT‑AAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGTTTATTAATTGCTTTTGCAGTACTTTTCTCAGCAGATTTc   <  2:218950/148‑1 (MQ=255)

                                                                                                                               gATTGCTTACATTGTTTTCT‑AAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGTTTATTAATTGCTTTTGCAGTACTTTTCTCAGCAGATTTCa  <  1:397394/148‑1 (MQ=255)

TGACATCCTTGTATCACTATGACAATTAATTGTTAAATACATGAATTTCTACATTTTATGAAAAAATCCATTTTTATTACAATTCAACACTTTATATGACAACTTCATTACAGTTACTTTTATTGTTGATTGCTTACATTGTTTTCTAAAAAAAATTTGTTATCATAATTAACGTTGAATAAAGAAAAAAATTAACTTGGGAGATAAAAATGGAATATAAAAAGATACTAATTCGTTTATTAATTGCTTTTGCAGTACTTTTCTCAGCAGATTTCA  >  CP000730/2679308‑2679583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A