Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F169 I1 R1
|
59 |
38.6 |
831760 |
90.8% |
755238 |
148.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
CP000730 |
2,787,510 |
T→C |
intergenic (+56/‑184) |
USA300HOU_2639 → / → USA300HOU_2640 |
hypothetical protein/BglG family transcriptional antiterminator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP000730 | 2,787,510 | 0 | T | C | 100.0%
| 77.4
/ NA
| 23 | intergenic (+56/‑184) | USA300HOU_2639/USA300HOU_2640 | hypothetical protein/BglG family transcriptional antiterminator |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (9/14); total (9/14) |
AATAAATCAAATAATACCAATACGCCTTCTAAACAAACACCTCAAGCACAATCACCTAAAAATTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCAT > CP000730/2787389‑2787626
|
aataaATCAAATAATACCAATACGCCTTCTAAACAAACACCTCAAGCACAATCACCTAAAAATTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGCGAGATATTTTTCTATTTGAAATATCt < 2:56116/148‑1 (MQ=255)
ataaATCAAATAATACCAATACGCCTTCTAAACAAACACCTCAAGCACAATCACCTAAAAATTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGCGAGATATTTTTCTATTTGAAATATCtt > 1:238162/1‑148 (MQ=255)
aaTCAAATAATACCAATACGCCTTCTAAACAAACACCTCAAGCACAATCACCTAAAAATTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGCGAGATATTTTTCTATTTGAAATATCTTAct < 1:54011/148‑1 (MQ=255)
tCAAATAATACCAATACGCCTTCTAAACAAACACCTCAAGCACAATCACCTAAAAATTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGCGAGATATTTTTCTATTTGAAATATCTTActgc > 2:74478/1‑148 (MQ=255)
taataCCAATACGCCTTCTAAACAAACACCTCAAGCACAATCACCTAAAAATTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGCGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTc < 1:198410/148‑1 (MQ=255)
taataCCAATACGCCTTCTAAACAAACACCTCAAGCACAATCACCTAAAAATTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGCGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTc < 1:323319/148‑1 (MQ=255)
aCCAATACGCCTTCTAAACAAACACCTCAAGCACAATCACCTAAAAATTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGCGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTa > 1:311093/1‑148 (MQ=255)
cAATACGCCTTCTAAACAAACACCTCAAGCACAATCACCTAAAAATTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGCGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTAtt > 1:250936/1‑148 (MQ=255)
tCTAAACAAACACCTCAAGCACAATCACCTAAAAATTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGCGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTc > 2:66004/1‑148 (MQ=255)
cacaATCACCTAAAAATTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACCGCAGCGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAATATCata < 2:354712/148‑1 (MQ=255)
aaataTTCATCAATTGCCCGACACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGCGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGc < 2:129759/148‑1 (MQ=255)
aaaaTTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGCGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCt > 2:46067/1‑148 (MQ=255)
aaaaTTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGCGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCt > 2:208620/1‑148 (MQ=255)
aaaaTTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGCGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCt < 2:270676/148‑1 (MQ=255)
aaaTTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGCGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCtt < 2:53186/148‑1 (MQ=255)
aaaTTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGCGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCtt < 1:339360/148‑1 (MQ=255)
gCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGCGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGAc < 1:172036/148‑1 (MQ=255)
tGATACGTCCACAAGTACTATTTGCATAAATTCAGAAAAACAGCAGCGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACgta < 1:22997/148‑1 (MQ=255)
tGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGCGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACgta < 2:196605/148‑1 (MQ=255)
aTACGTCCACAAGTACCAGATGCATAAATTCAGAACAACAGCAGCGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACgtatg < 2:175080/148‑1 (MQ=255)
gTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGCGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGtata > 1:184676/1‑148 (MQ=255)
gTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGCGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGtata > 1:273617/1‑148 (MQ=255)
tACCAGATGCATAAATTCAGAAAAACAGCAGCGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCAt < 1:319508/148‑1 (MQ=255)
|
AATAAATCAAATAATACCAATACGCCTTCTAAACAAACACCTCAAGCACAATCACCTAAAAATTAATCATTTGCCTGATACGTCCACAAGTACCAGATGCATAAATTCAGAAAAACAGCAGTGAGATATTTTTCTATTTGAAATATCTTACTGCTGTTCTTTATTTGCATAATTCGCACTCTGTTAAAATCATATAAAAGTTCGCTTTAATGTCATTGACGTATGTATAAGCTCTCAT > CP000730/2787389‑2787626
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
