Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F184 I1 R1 31 33.8 680504 95.8% 651922 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 2,544,339 C→T V189V (GTC→GTT)  hlgC → gamma hemolysin component C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,544,3390CT100.0% 147.8 / NA 41V189V (GTC→GTT) hlgCgamma hemolysin component C
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/18);  total (23/18)

GGATACAATATCGGTGGTAATTTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTCAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAACCTCATAGTAAAGATCCTAGAGATTATTTCGTTCCAGACAGTGAGTTACCACCTCTTGTACAAAGTGGATT  >  CP000730/2544193‑2544482
                                                                                                                                                  |                                                                                                                                               
ggATACAATATCGGTGGTAATTTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTaa                                                                                                                                               >  1:207/1‑149 (MQ=255)
ggATACAATATCGGTGGTAATTTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTaa                                                                                                                                               >  1:196516/1‑149 (MQ=255)
 gATACAATATCGGTGGTAATTTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTaaa                                                                                                                                              >  2:176534/1‑149 (MQ=255)
  aTACAATATCGGTGGTAATTTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAg                                                                                                                                             >  2:23355/1‑149 (MQ=255)
   tACAATATCGGTGGTAATTTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGc                                                                                                                                            <  2:29493/149‑1 (MQ=255)
         aacGGTGGTAATTTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAattc                                                                                                                                      <  2:72037/147‑1 (MQ=255)
           cGGTGGTAATTTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAattcat                                                                                                                                    >  2:17222/1‑149 (MQ=255)
                       ccAATCAGCCCCAGCACTAGGTCGTAATGGATCATTTAACTATTCTAAATCCATTAGCTATACACAACAAAATTATGTAAGTAAAATAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAAt                                                                                                                        <  2:42971/149‑1 (MQ=255)
                        cAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACGATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATc                                                                                                                       <  1:252037/149‑1 (MQ=255)
                            cAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGt                                                                                                                   >  2:120887/1‑149 (MQ=255)
                                      aCTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAg                                                                                                         <  1:80268/149‑1 (MQ=255)
                                           gtggtAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCttt                                                                                                    <  2:228613/149‑1 (MQ=255)
                                           gtggtAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCttt                                                                                                    >  1:326167/1‑149 (MQ=255)
                                            tggtAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTg                                                                                                   <  2:226926/149‑1 (MQ=255)
                                            tggtAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTg                                                                                                   <  2:55145/149‑1 (MQ=255)
                                                     aTCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGatt                                                                                          <  2:137523/149‑1 (MQ=255)
                                                        aTTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGatttat                                                                                       <  2:333000/149‑1 (MQ=255)
                                                                  tCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTa                                                                             >  2:312460/1‑149 (MQ=255)
                                                                       aTCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAAc                                                                        >  1:34280/1‑149 (MQ=255)
                                                                        tagATGAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAAACAAGTAAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAAcc                                                                       <  2:184487/147‑1 (MQ=255)
                                                                        tCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAAcc                                                                       <  2:292904/149‑1 (MQ=255)
                                                                          gATTAGCTATACACAACAATATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAACCTc                                                                     <  2:257267/149‑1 (MQ=255)
                                                                          gATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAACCTc                                                                     <  2:280964/149‑1 (MQ=255)
                                                                             tAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAACCTCATa                                                                  <  1:295801/149‑1 (MQ=255)
                                                                              aGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAACCTCATAg                                                                 <  2:26994/149‑1 (MQ=255)
                                                                                   taCACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAACCTCATAGTAAAg                                                            >  1:65234/1‑149 (MQ=255)
                                                                                      acaacaAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAACCTCATAGTAAAGATc                                                         >  1:83526/1‑149 (MQ=255)
                                                                                                  tGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAACCTCATAGTAAAGATCCTAGAGattatt                                             >  2:10344/1‑149 (MQ=255)
                                                                                                      aGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAACCTCATAGTAAAGATCCTAGAGATTATTTCGt                                         >  1:58597/1‑149 (MQ=255)
                                                                                                        tGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGCCTACAAACCTCATAGTAAAGATCCTAGAGATTATTTCGTTc                                       <  1:176534/149‑1 (MQ=255)
                                                                                                         gAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAACCTCATAGTAAAGATCCTAGAGATTATTTCGTTcc                                      >  2:93124/1‑149 (MQ=255)
                                                                                                          aaGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAACCTCATAGTAAAGATCCTAGAGATTATTTCGTTCCa                                     >  1:14238/1‑149 (MQ=255)
                                                                                                            gTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAACCTCATAGTAAAGATCCTAGAGATTATTTCGTTCCAGa                                   >  1:107989/1‑149 (MQ=255)
                                                                                                             tAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAACCTCATAGTAAAGATCCTAGAGATTATTTCGTTCCAGAc                                  >  1:214686/1‑149 (MQ=255)
                                                                                                                aacaacAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGCTTTATTTGTAGGATCCACAGCTCATGGTAAAGATCCTAGAGATTATTTCGTTCCAGACAGt                               >  1:220143/1‑149 (MQ=255)
                                                                                                                         aCTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAACCTCATAGTAAAGATCCTAGAGATTATTTCGTTCCAGACAGTGAGTTacca                      <  1:91219/149‑1 (MQ=255)
                                                                                                                                         aTGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAACCTCATAGTAAAGATCCTAGAGATTATTTCGTTCCAGACAGTGAGTTACCACCTCTTGTACAAAGTg      >  1:126658/1‑149 (MQ=255)
                                                                                                                                           ggggCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAACCTCATAGTAAAGATCCTAGAGATTATTTCGTTCCAGACAGTGAGTTACCACCTCTTGTACAAAGTGGa    >  2:192692/1‑149 (MQ=255)
                                                                                                                                           ggggCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAACCTCATAGTAAAGATCCTAGAGATTATTTCGTTCCAGACAGTGAGTTACCACCTCTTGTACAAAGTGGa    >  2:120044/1‑149 (MQ=255)
                                                                                                                                             ggCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAACCTCATAGTAAAGATCCTAGAGATTATTTCGTTCCAGACAGTGAGTTACCACCTCTTGTACAAAGTGGAtt  >  2:144305/1‑149 (MQ=255)
                                                                                                                                             ggCGTTAAAGCGAATCCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAACCTCATAGTAAAGATCCTAGAGATTATTTCGTTCCAGACAGTGAGTTACCACCTCTTGTACAAAGTGGAtt  >  2:8164/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
GGATACAATATCGGTGGTAATTTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTCAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAACCTCATAGTAAAGATCCTAGAGATTATTTCGTTCCAGACAGTGAGTTACCACCTCTTGTACAAAGTGGATT  >  CP000730/2544193‑2544482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A