breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 3,790 G→A A32T (GCA→ACA)  yaaA → hypothetical protein
RA 39,378 C→A D586Y (GAT→TAT)  mecA ← penicillin binding protein 2 prime
RA 76,969 G→A intergenic (+1027/‑107) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 195,286 T→A intergenic (‑97/‑82) USA300HOU_0183 ← / → USA300HOU_0184 hypothetical protein/hypothetical protein
RA 355,466 G→A P412S (CCA→TCA)  brnQ1 ← LIVCS family branched chain amino acid:cation symporter
RA 396,540 G→A A208T (GCA→ACA)  USA300HOU_0364 → possible lipoprotein
RA 607,884 G→A R209* (CGA→TGA)  dck ← deoxynucleoside kinase
RA 629,681 C→A L84I (CTA→ATA)  hxlA → possible 3‑hexulose‑6‑phosphate synthase
RA 665,392 T→G L79* (TTA→TGA)  iolS → possible acetytransferase
RA 707,910 A→G intergenic (‑1/‑121) mntB ← / → sirR ABC superfamily ATP binding cassette transporter, ABC protein/possible metal‑dependent transcriptional regulator
RA 874,718 A→C K541N (AAA→AAC clfA → fibrinogen‑binding protein
RA 1,064,254 Δ1 bp coding (235/1218 nt) brpA ← LytR family transcriptional regulator
RA 1,233,504 T→A V470D (GTT→GAT)  prkC → non‑specific serine/threonine protein kinase
RA 1,416,458 A→T I216N (ATT→AAT)  oppD ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
RA 1,440,085 G→T intergenic (‑45/‑891) cspA ← / → xpaC cold shock protein CspA/5‑bromo‑4‑chloroindolyl phosphate hydrolysis protein XpaC
RA 1,513,930 A→T L413F (TTA→TTT USA300HOU_1379 → hypothetical membrane protein
RA 1,646,189 G→C A155G (GCA→GGA)  pepP ← M24B family Xaa‑Pro dipeptidase
RA 1,744,362 G→A H104Y (CAC→TAC)  apt ← adenine phosphoribosyltransferase
MC JC 1,747,382 (TAC)3→2 coding (2065‑2067/2280 nt) secDF ← RND superfamily resistance‑nodulation‑cell division protein:proton (H+) antiporter
RA 1,761,889 C→A D388Y (GAT→TAT)  folC ← tetrahydrofolate synthase
RA 1,963,768 C→G V365L (GTA→CTA)  hemG ← protoporphyrinogen oxidase
RA 2,010,363 C→T C146Y (TGT→TAT)  ygaD ← ABC superfamily ATP binding cassette transporter, membrane protein
RA 2,027,672 C→A G92V (GGC→GTC)  vraS ← sensor histidine kinase VraS
RA 2,241,539 T→C intergenic (‑182/+284) murZ ← / ← fbaA UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase/fructose‑bisphosphate aldolase
RA 2,279,705 G→A intergenic (+1373/+6659) mtlD → / ← glmM mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase
RA 2,289,262 C→G W76C (TGG→TGC ybbP ← hypothetical membrane protein
RA 2,352,815 G→A A92V (GCA→GTA)  rpsI ← ribosomal protein S9
RA 2,454,626 A→T L48L (CTA→CTT USA300HOU_2315 → hypothetical membrane protein
RA 2,532,298 A→T T15T (ACA→ACT murN → FemAB family peptidoglycan biosynthesis protein
RA 2,544,339 C→T V189V (GTC→GTT hlgC → gamma hemolysin component C
RA 2,709,576 C→T G451S (GGT→AGT)  oatA ← possible acetyltransferase

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 512620 =37 (1.110)38 (1.560)
+40 bp
24/216 0.1 58.5% intergenic (+688/‑173) recR/rrs1 recombination protein RecR/16S ribosomal RNA
?CP000730 1998278 = NA (NA)intergenic (‑6/+5) USA300HOU_t0050/rrf4 tRNA‑Val/5S ribosomal RNA
* ? CP000730 2085296 =21 (0.630)6 (0.200) 6/268 2.9 21.1% intergenic (‑558/‑400) mapW2/USA300HOU_1944 cell surface protein MapW2/hypothetical protein
?CP000730 = 2128343 26 (0.860)intergenic (+57/+1062) int/hlgB bacteriophage integrase/possible leukocidin subunit