Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F22 I1 R1	21	115.3	2512058	90.2%	2265876	148.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,454,626	A→T	L48L (CTA→CTT)	USA300HOU_2315 →	hypothetical membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,454,626	0	A	T	99.2%	374.8 / NA	117	L48L (CTA→CTT)	USA300HOU_2315	hypothetical membrane protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base T (51/65); total (51/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 117 total aligned reads displayed.

AATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTACCTTTAATTATTCTAA  >  CP000730/2454482‑2454765

   gaagaaCAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCa                                                                                                                                        >  1:881855/1‑148 (MQ=255)

   gaagaaCAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCa                                                                                                                                        >  1:383811/1‑148 (MQ=255)

    aagaaCAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATTTTTGAAGTTACAGATCTTGCACCAt                                                                                                                                       >  1:1154511/1‑148 (MQ=255)

    aagaaCAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCAt                                                                                                                                       >  1:56077/1‑148 (MQ=255)

     agaaCAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTGCAGATCTTGCACCAtt                                                                                                                                      >  2:680523/1‑148 (MQ=255)

     agaaCAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCAtt                                                                                                                                      >  2:1215755/1‑148 (MQ=255)

     agaaCAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCAtt                                                                                                                                      >  2:501860/1‑148 (MQ=255)

      gaaCAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTa                                                                                                                                     >  1:71183/1‑148 (MQ=255)

      gaaCAATAAAATTTCTGGTTTTCAATGGGCAATGAAGATTTCAGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATAATTGAAGTTACAGCTCTTGCACCATTa                                                                                                                                     >  1:790729/1‑148 (MQ=255)

        acaatacaatTTCTCGTTTTCAATGGTCAATGACGATTTTCGTCGTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAt                                                                                                                                   <  2:436857/148‑1 (MQ=255)

        aCAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAt                                                                                                                                   <  2:331921/148‑1 (MQ=255)

        aCAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAt                                                                                                                                   <  1:524160/148‑1 (MQ=255)

        aCAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAt                                                                                                                                   <  1:944120/148‑1 (MQ=255)

        aCAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAt                                                                                                                                   <  1:141147/148‑1 (MQ=255)

           aTAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑tgc                                                                                                                               <  1:636483/148‑1 (MQ=255)

           aTAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑tgc                                                                                                                               <  1:481523/148‑1 (MQ=255)

           aTAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑tgc                                                                                                                               <  1:249234/148‑1 (MQ=255)

           aTAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCCTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGGGACAGATCTTGCACCATTAAT‑tgc                                                                                                                               <  2:1076819/148‑1 (MQ=255)

               aaTTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCa                                                                                                                           >  1:263813/1‑148 (MQ=255)

               aaTTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCa                                                                                                                           >  1:30670/1‑148 (MQ=255)

               aaTTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCa                                                                                                                           <  2:1183222/148‑1 (MQ=255)

                aTTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCaa                                                                                                                          >  1:1106778/1‑148 (MQ=255)

                    cTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCAt                                                                                                                      <  2:79778/148‑1 (MQ=255)

                       cttttCAATGGGCAATGACGTTTTGCGTCTTCTTTGTCATTACAATGTCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTg                                                                                                                   <  1:151305/147‑1 (MQ=255)

                         tttCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCAttttga                                                                                                                 >  2:187263/1‑145 (MQ=255)

                         tttCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TACTGCACTCATTTGta                                                                                                                 >  2:1030013/1‑148 (MQ=255)

                          ttCAATGGGCAATGACCATTTTCGTCTTCTTTGTCAGTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGtat                                                                                                                <  2:1110158/148‑1 (MQ=255)

                            cAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATAc                                                                                                              >  2:744474/1‑148 (MQ=255)

                             agtGGGCTATGACGATTTTCGTCTGCGTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACt                                                                                                             <  2:889989/146‑1 (MQ=255)

                             aaTGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACt                                                                                                             <  2:2445/148‑1 (MQ=255)

                              aTGGGCAATGACGATTTTCGTCTTCTTTGTCCATACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTCAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTc                                                                                                            >  2:572151/1‑148 (MQ=255)

                                 gccAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGtt                                                                                                         >  2:1087554/3‑148 (MQ=255)

                                  gCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGttt                                                                                                        >  1:100084/1‑148 (MQ=255)

                                   cAATGACTCTTTTCGTCTTCTTTGTTATTACAATGGCGTTATCCATTATTCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGtttt                                                                                                       <  2:42589/148‑1 (MQ=255)

                                     aTGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCa                                                                                                     >  1:415040/1‑148 (MQ=255)

                                     aTGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCa                                                                                                     <  1:1142012/148‑1 (MQ=255)

                                     aTGACCATCTTCGTCTTCTTTGTCATTACAATGGCGGTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGTTCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCa                                                                                                     <  2:1068531/148‑1 (MQ=255)

                                                   cttcttTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTc                                                                                       >  1:123331/1‑148 (MQ=255)

                                                   cttcttTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTc                                                                                       >  1:671594/1‑148 (MQ=255)

                                                        ttGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACt                                                                                  >  2:1160731/1‑148 (MQ=255)

                                                           tCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCATTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGc                                                                               <  1:885295/148‑1 (MQ=255)

                                                           tCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGc                                                                               <  1:885503/148‑1 (MQ=255)

                                                                  aaTGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTa                                                                        >  1:1015963/1‑148 (MQ=255)

                                                                  aaTGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTa                                                                        >  1:1187505/1‑148 (MQ=255)

                                                                   aTGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTaa                                                                       >  1:390405/1‑148 (MQ=255)

                                                                   aTGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTaa                                                                       >  2:439390/1‑148 (MQ=255)

                                                                        gTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATtc                                                                  <  1:775858/148‑1 (MQ=255)

                                                                         ttATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATtct                                                                 >  2:515620/1‑148 (MQ=255)

                                                                         ttATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATtct                                                                 <  1:411066/148‑1 (MQ=255)

                                                                          tATCCATTATGCTCAGAGATTTCCAGACTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACAATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATtctc                                                                >  2:26663/1‑148 (MQ=255)

                                                                             ccATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTGGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAAt                                                             >  1:73720/1‑148 (MQ=255)

                                                                                 tATGCTCAGAGATTTCCCGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGc                                                         <  1:38199/148‑1 (MQ=255)

                                                                                  aTGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGcc                                                        >  2:1114431/1‑148 (MQ=255)

                                                                                   tGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTGCAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCt                                                       <  1:680523/148‑1 (MQ=255)

                                                                                   tGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCTTTTTCAAAGATACAACGGTCCAACTTGCAGTTTTGAACTTCTCAATCAGCCt                                                       >  1:283930/1‑148 (MQ=255)

                                                                                   tGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCt                                                       <  1:501860/148‑1 (MQ=255)

                                                                                   tGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCAGTAAT‑TGCTTCAAGCATTTGTATACTCGTTTGCAAATATAAAAAGGTCCACCTGTCAGGTTTAAAAATATCAAGCGGCCt                                                       >  1:258552/1‑148 (MQ=255)

                                                                                   tGCTCAGAGATGTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCt                                                       <  1:1215755/148‑1 (MQ=255)

                                                                                     cTCAGAGATTTCCAGTCCATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGTAGGTTTAAAATTCTCAATCAGCCTGa                                                     <  1:661323/148‑1 (MQ=255)

                                                                                     cTCAGACATTTCCAGTCTACATTTGTTGTTAAATACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGa                                                     <  2:615936/148‑1 (MQ=255)

                                                                                      tCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCGAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGaa                                                    <  1:546906/148‑1 (MQ=255)

                                                                                        agagATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACACGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAg                                                  >  2:1014241/1‑148 (MQ=255)

                                                                                          agaTTTCCATTCTATACTTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTa                                                <  2:1166688/148‑1 (MQ=255)

                                                                                          agaTTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTa                                                <  2:415040/148‑1 (MQ=255)

                                                                                               tCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGa                                           <  2:774436/148‑1 (MQ=255)

                                                                                               tCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGa                                           <  1:524153/148‑1 (MQ=255)

                                                                                                  aGTCTATAATTGGTGTCAAACATTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACg                                        <  2:383847/148‑1 (MQ=255)

                                                                                                  aGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACg                                        <  2:451838/148‑1 (MQ=255)

                                                                                                   gTCTATAATTGGTTTCAAACATTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGt                                       <  2:160824/148‑1 (MQ=255)

                                                                                                   gTCTATAATTGGTGTCAAACACTTTATATTTGACGTTCACGATCTTGCTCCATTAAT‑TGCTGCCGTCGTTTTTATACTCGTTGTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGt                                       <  2:1235982/148‑1 (MQ=255)

                                                                                                   gTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTCCAGATC‑ATGAGCATTTTTAGGCTTGTCTCATTTGTATACTCGTTTTTAAATATAAAATGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGt                                       <  2:879857/148‑1 (MQ=255)

                                                                                                   gTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCCCCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGTTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGt                                       <  2:1236649/148‑1 (MQ=255)

                                                                                                   gTCTAGAATTGGTTGCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTATAAAAAAAAAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGt                                       <  2:899920/148‑1 (MQ=255)

                                                                                                   gTCCATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATCCTCGTCTTAAAATATAAAAAGTTCCAACTTGCGTGTTTAAAATTCTAAATCAGCTTGTAAGTAATTGAACGt                                       <  2:1202979/148‑1 (MQ=255)

                                                                                                    tctttcaTTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTAGACTCGTTTTCAAATATAAAAAGTTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTc                                      <  1:1051401/142‑1 (MQ=255)

                                                                                                    tCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTc                                      >  1:767744/1‑148 (MQ=255)

                                                                                                      tataATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGTTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTa                                    <  2:30670/148‑1 (MQ=255)

                                                                                                      tataATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTa                                    <  2:18954/148‑1 (MQ=255)

                                                                                                        tacttGGTGGCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTAtt                                  <  1:268852/145‑1 (MQ=255)

                                                                                                          aTTGGTGTCAAACACTTTATAGTTGAAGTTACAGATCCTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAATTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGc                                <  2:666597/148‑1 (MQ=255)

                                                                                                              gtgtCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAACTTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGc                            <  2:881855/148‑1 (MQ=255)

                                                                                                              gtgtCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGCAAGTAATTGAACGTCTCTTGCTAgt                            >  2:76863/1‑147 (MQ=255)

                                                                                                                gtCAAACACTTTATATTTGAAGTTACAGATCTTGCACCCTTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGACAGTAATTGAACGTCTATTGCTAGcgt                          >  1:358300/1‑146 (MQ=255)

                                                                                                                gtCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCtt                          >  2:440519/1‑148 (MQ=255)

                                                                                                                gtCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCtt                          >  2:1246741/1‑148 (MQ=255)

                                                                                                                gtCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCtt                          >  1:924233/1‑148 (MQ=255)

                                                                                                                 tCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCttt                         >  1:478313/1‑148 (MQ=255)

                                                                                                                  caaattcTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTa                        <  2:737863/142‑1 (MQ=255)

                                                                                                                  cAAACACTTTATAGTTTAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAATTTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCttgattgg                        <  2:770574/148‑8 (MQ=255)

                                                                                                                     acacTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAAtt                     >  2:218396/1‑148 (MQ=255)

                                                                                                                      cacTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAAttt                    <  2:123331/148‑1 (MQ=255)

                                                                                                                      cacTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAAttt                    <  2:671594/148‑1 (MQ=255)

                                                                                                                       ccTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGCTCCCACTTGCGGGTTAGCAACTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAAtttt                   <  2:1003741/147‑1 (MQ=255)

                                                                                                                        cTTTATATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTa                  <  2:100084/148‑1 (MQ=255)

                                                                                                                          ttATATTGGAAGTTACAGATCGTGCATCATGAAG‑TGCGGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGGAAGTAATTGTATGGCTATTGCTAGCTTTAATTTTAcc                <  1:1184121/148‑1 (MQ=255)

                                                                                                                          ttAAATTTGAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGCAATTGAACGTCTATTGCTAGCTTTAATTTTAcc                <  2:688905/148‑1 (MQ=255)

                                                                                                                                  taaGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTACCTTTAatta        <  1:885119/147‑1 (MQ=255)

                                                                                                                                  gAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTACCTTTAatta        <  1:699233/148‑1 (MQ=255)

                                                                                                                                  gAAGTTACAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTACCTTTAatta        >  2:908909/1‑148 (MQ=255)

                                                                                                                                        aCAGATCTTGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTACCTTTAATTGTTCTaa  >  1:589351/1‑148 (MQ=255)

AATGAAGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAAT‑TGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTACCTTTAATTATTCTAA  >  CP000730/2454482‑2454765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A