Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A19 F166 I0 R1	49	63.8	1389156	94.9%	1318309	140.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	597,770	A→T	100%	V168V (GTA→GTT)	tufA →	elongation factor EF1A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	597,770	0	A	T	98.6%	231.5 / ‑5.4	77	V168V (GTA→GTT)	tufA	elongation factor EF1A
Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (43/32); minor base G (1/1); total (44/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCACGTAACGTTGGTGTACCAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTACCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATTCCAACTCCAGAACGTGATTCTGACAAACCATTCATGATGC  >  CP000730/597633‑597906

tCACGTAACGTTGGTGTACCAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCt                                                                                                                                       >  2:209460/1‑141 (MQ=255)

tCAAGTAACGTTGGTGTACCAGCATTAGTAGTATTCTTAAACAAAGTTGAGGTGGTTGGCGGTGAAGAGTGATTAGAATTAGTAGAAATGGAAGTGCGTGACTTATTAAGCGAATATGACTTCCTAGGTGGCGGTGTTCCt                                                                                                                                       >  2:39453/1‑141 (MQ=255)

   cGTAACGTTGGTGTACCAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATGATTAGAATTAGTAGAAATGGAAGGTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTGCCTTTa                                                                                                                                    >  1:43869/1‑141 (MQ=255)

       aCGTTGGTGTACCAGCATTATTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCg                                                                                                                                <  2:556044/141‑1 (MQ=255)

        cGTTGGTGTACCAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGc                                                                                                                               >  1:236060/1‑141 (MQ=255)

        cGTTGGTGTACCAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGc                                                                                                                               >  2:575566/1‑141 (MQ=255)

        cGTTGGTGTACCAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGc                                                                                                                               <  2:29083/141‑1 (MQ=255)

        cGTTGGTGTACCAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGc                                                                                                                               >  2:596981/1‑141 (MQ=255)

        agTTGGTGTACCAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGc                                                                                                                               <  2:417597/140‑1 (MQ=255)

           acgctcttccgatctagtagTATTCTTAAACAACGTTGCACTGGTTGAGGCTACCCTAGCCGTTGAATTAGTAGAAATGGAAGTTCGTTACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTgg                                                                                                                            <  2:146514/127‑1 (MQ=255)

            ggTGTACCAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCACAGGCGACGATGTTCCTGTAATCGCTGGt                                                                                                                           >  2:344331/1‑141 (MQ=255)

              tgtACCAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTc                                                                                                                         >  1:323551/1‑141 (MQ=255)

                 aCCAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCGGc                                                                                                                      >  2:53511/1‑141 (MQ=255)

                 aCCAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGc                                                                                                                      <  2:373257/141‑1 (MQ=255)

                  ccAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCa                                                                                                                     <  2:689454/141‑1 (MQ=255)

                  ccAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAAGATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCa                                                                                                                     <  1:305844/141‑1 (MQ=255)

                   cAGCCTTTGTAGTATTATGACAAAAAGGTGACATGGTTGACGATGAAGAATTATTAGAATTATTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCAt                                                                                                                    <  2:653007/141‑1 (MQ=255)

                   cAGCATTAGTAGTATTCTGAAACAAAGTTGACATGGTTGCCGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCAt                                                                                                                    <  2:167040/141‑1 (MQ=255)

                          agtagtATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGc                                                                                                             >  1:314362/1‑141 (MQ=255)

                            tagtaTTCTTAAACAAAGTTGACATGGTTGGCGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCtt                                                                                                           <  1:97039/141‑1 (MQ=255)

                            tagtaTTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCtt                                                                                                           >  1:145384/1‑141 (MQ=255)

                            tagtaTTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCtt                                                                                                           <  1:463947/141‑1 (MQ=255)

                             agtaTTCGTAAACAAAGTTGACATGGTTGATGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCttt                                                                                                          <  2:69987/141‑1 (MQ=255)

                              gtaTTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTa                                                                                                         <  2:467540/141‑1 (MQ=255)

                                aTTCTTAAACAAAGTTTCCATGGTTGACGATGAAGAATTATTAGCATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCACAGTGCCAGCAGTAAAAGCTTTAGa                                                                                                       <  2:668963/141‑1 (MQ=255)

                                    ttAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTaagatcggaagagcac                                                                                                   >  1:146514/1‑127 (MQ=255)

                                    ttAAACAAAGTGTACATTGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATTGCTGGTTCAGCATTAAAAGCTTTAGAAGGc                                                                                                   <  1:432347/141‑1 (MQ=255)

                                        aCAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATg                                                                                               >  1:407677/1‑141 (MQ=255)

                                          aaaGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGcg                                                                                             >  1:520084/1‑140 (MQ=255)

                                           aagtgtaCATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTCATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTc                                                                                            <  2:497801/135‑1 (MQ=255)

                                             gTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTATAAGCTTTAGAAGGCGATGCTcta                                                                                          >  2:99613/1‑139 (MQ=255)

                                             gTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGGTCGTGACTTATTGAGCGTATATGACTTCGGAGGTGACGAGGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGcgcca                                                                                          >  2:592881/1‑137 (MQ=255)

                                              ttGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAAt                                                                                         >  2:101948/1‑141 (MQ=255)

                                                gACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATAc                                                                                       >  1:595892/1‑141 (MQ=255)

                                                  cATGGGTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACga                                                                                     <  1:612202/141‑1 (MQ=255)

                                                   aTGGTTTACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACgaa                                                                                    <  1:270615/141‑1 (MQ=255)

                                                   aTGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACgaa                                                                                    <  1:587327/141‑1 (MQ=255)

                                                   aTGGTTGAAGATGAATAATGATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACgaa                                                                                    <  1:261403/141‑1 (MQ=255)

                                                   aTGGCTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAATTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACgaa                                                                                    <  1:261366/141‑1 (MQ=255)

                                                      gTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACgaagaa                                                                                 >  1:617555/1‑141 (MQ=255)

                                                      gTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACgaagaa                                                                                 <  1:669257/141‑1 (MQ=255)

                                                          aCGATGAAGCATTATTAGAATTAGTAGAAATGGAAGGTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGaaaaaa                                                                             <  2:72055/141‑1 (MQ=255)

                                                                  gaaTTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGaa                                                                     >  2:676192/1‑141 (MQ=255)

                                                                  gaaTTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAAGCTTAGaa                                                                     >  1:600793/1‑141 (MQ=255)

                                                                        ttggAAGTAGTGGAAATGGAAGTTGCTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTGCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATg                                                               <  2:20271/138‑1 (MQ=255)

                                                                          aGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGa                                                             >  1:431046/1‑141 (MQ=255)

                                                                          aGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGa                                                             >  1:522720/1‑141 (MQ=255)

                                                                          aGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCACAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGa                                                             >  1:336247/1‑141 (MQ=255)

                                                                          aGAAGTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGa                                                             <  1:215797/141‑1 (MQ=255)

                                                                             aTTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGc                                                          <  2:497575/141‑1 (MQ=255)

                                                                             aTTAGTAGAAATGGAAGTGCGTGTCTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGc                                                          <  2:155832/141‑1 (MQ=255)

                                                                              ttAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCCGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAgat                                                         >  2:80374/1‑139 (MQ=255)

                                                                               tagtagAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTg                                                        >  2:10809/1‑141 (MQ=255)

                                                                                 gtagAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTa                                                      >  1:78837/1‑141 (MQ=255)

                                                                                 gtagAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTa                                                      >  1:653426/1‑141 (MQ=255)

                                                                                 gtagAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTa                                                      >  1:151853/1‑141 (MQ=255)

                                                                                   agAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGa                                                    >  1:256106/1‑141 (MQ=255)

                                                                                    gAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGCTCAGCATTAAAAGCTTTGGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTCTAGAt                                                   >  2:77757/1‑141 (MQ=255)

                                                                                           aaGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAACGACACTCTCATTACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGGTACTTACa                                            >  1:193282/1‑141 (MQ=255)

                                                                                             gTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGACGCAAAAATCTTAGAATTAAGAGCAGCTGTAGCGACTTGCAtt                                          >  2:677804/1‑141 (MQ=255)

                                                                                              ttCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGGTGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACAtta                                         >  2:138707/1‑140 (MQ=255)

                                                                                                cGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGTTGCTCAAGACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATTCCa                                       >  1:159832/1‑141 (MQ=255)

                                                                                                cGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATTCCa                                       >  1:406775/1‑141 (MQ=255)

                                                                                                   gACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATTCCAACt                                    >  2:597538/1‑141 (MQ=255)

                                                                                                         ttaAGCGAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATTCCAACTCCAGaa                              <  1:545072/141‑1 (MQ=255)

                                                                                                         ttaAGCGAATATGACTGCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATTCCAACTCCAGaa                              <  1:285200/141‑1 (MQ=255)

                                                                                                             gCGAATATGACTTCCCAGGCGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATTCCAACTCCAGAACGTg                          <  2:133657/141‑1 (MQ=255)

                                                                                                               gAATATGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATTCCAACTCCAGAACGTGAt                        >  1:56292/1‑141 (MQ=255)

                                                                                                                   atGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATTCCAACTCCAGAACGTGATTCTg                    <  2:520084/141‑1 (MQ=255)

                                                                                                                    tGACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGAGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATCCAAACTCCAGCAAGTGATTCTGa                   >  2:502020/1‑141 (MQ=255)

                                                                                                                     gACTTCCCAGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATGTTAGAATTAAGGGAAGCTGTAGATACTTACAATCCAACTCCAGCACgggtctgagaa                  >  2:52900/1‑131 (MQ=255)

                                                                                                                             aGGTGACGATGTTCCTGTAATCGCTGGTTCAGCCTTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAAACGTAGAATTAATGGAAGCTGCAGATACTTACATTCCAACTCCACAACGTGCTTTTGGCAAAGCAtt          >  2:79515/1‑141 (MQ=255)

                                                                                                                             aGGTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGGATTAATGGAAGCTGTAGATACTTACATTCCAACTACAGAACGTGATTCTGACaaattatt          >  2:171581/1‑136 (MQ=255)

                                                                                                                               gTGACGATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATTCCAACTCCAGAACGTGATTCTGACAAACCATTCa        >  1:375000/1‑141 (MQ=255)

                                                                                                                                  accaTGTTCCGGTAATCGCTGGTTCAGAATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATTCCAACTCCAGAACGTGTTTCTGACAAACCATTCatga     <  1:621567/138‑1 (MQ=255)

                                                                                                                                    gATGTTCCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATTCCAACTCCAGAACGTGATTCTGACAAACCATTCatgatg   >  2:13686/1‑141 (MQ=255)

                                                                                                                                     aTGTTCCTGTAATCGCTGGTTCAGCATCAAACGCTTGAGGGGGCGATTGTCAATACGAAGAAATAATCTTAGAATTAATGGAAGCTGTAGATACTTACATTCCAACTCCAGAACGTGATTCTGACAAACCATTCATGATGc  <  1:470215/141‑1 (MQ=255)

TCACGTAACGTTGGTGTACCAGCATTAGTAGTATTCTTAAACAAAGTTGACATGGTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAATATGACTTCCCAGGTGACGATGTACCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAAGGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATTCCAACTCCAGAACGTGATTCTGACAAACCATTCATGATGC  >  CP000730/597633‑597906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A