Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F67 I1 R1	13	62.5	1286300	92.5%	1189827	135.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	603,961	G→A	M465I (ATG→ATA)	araB →	ribulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	603,961	0	G	A	100.0%	221.7 / NA	63	M465I (ATG→ATA)	araB	ribulokinase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/41); total (22/41)

ACTAAGATGATTATGCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATGGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTTATACGAAATTAATCCAGAAGCGGAAAA  >  CP000730/603824‑604095

aCTAAGATGATTATGCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGAt                                                                                                                                     <  2:254087/141‑1 (MQ=255)

 cTAAGATGATTATGCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATa                                                                                                                                    >  1:56141/1‑141 (MQ=255)

 cTAAGATGATTATGCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATa                                                                                                                                    >  1:154397/1‑141 (MQ=255)

 cTAAGATGATTATGCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTAATGAATAAAAAACTAATTGTTATAGATa                                                                                                                                    <  1:576932/141‑1 (MQ=255)

      atgatTATGCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAg                                                                                                                               <  1:172555/141‑1 (MQ=255)

         atTATGCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTAt                                                                                                                            >  1:97360/1‑141 (MQ=255)

             tGCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCAc                                                                                                                        <  2:535716/141‑1 (MQ=255)

              gCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCAcc                                                                                                                       <  2:610317/141‑1 (MQ=255)

              gCAAAAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCAcc                                                                                                                       <  2:610389/141‑1 (MQ=255)

                  cAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCa                                                                                                                   >  1:177805/1‑141 (MQ=255)

                   aGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCaa                                                                                                                  <  2:318912/141‑1 (MQ=255)

                     tATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATa                                                                                                                >  1:32601/1‑141 (MQ=255)

                         aaGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTACAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGgcg                                                                                                            >  1:176130/1‑141 (MQ=255)

                           gATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCgca                                                                                                          <  2:92749/141‑1 (MQ=255)

                            agtgacATATAGAAATAAAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTGCTGATGGATATCTATGCGAATGCACTGAATAAAAAACTAGTTGTTATAGATAGTGAGTAGGCACCAGCAATAGGCgcag                                                                                                         <  2:154102/136‑1 (MQ=255)

                            aTTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCgcag                                                                                                         <  2:225894/141‑1 (MQ=255)

                            aTTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCgcag                                                                                                         <  2:149194/141‑1 (MQ=255)

                            aTTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGCACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCgcag                                                                                                         <  2:593193/141‑1 (MQ=255)

                              tGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCgcagca                                                                                                       <  1:359651/141‑1 (MQ=255)

                               ggCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCaa                                                                                                      <  2:40182/141‑1 (MQ=255)

                                   tatGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATAtt                                                                                                  <  2:359120/141‑1 (MQ=255)

                                     tGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAg                                                                                                <  1:639791/141‑1 (MQ=255)

                                           tAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGcag                                                                                          <  2:185014/141‑1 (MQ=255)

                                           tAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGcag                                                                                          <  2:412646/141‑1 (MQ=255)

                                              aaaaGGTACTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAAGAAAAAACTAATTGTTATAGATAGTGAGTATGTACCAGCAATAGGCGCAGCAATATTAGGTGcagtca                                                                                       <  1:554310/141‑1 (MQ=255)

                                                aaGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGcagtcagt                                                                                     <  2:608849/141‑1 (MQ=255)

                                                aaGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGcagtcagt                                                                                     >  1:198225/1‑141 (MQ=255)

                                                     aTTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGtggtgg                                                                                >  2:309580/1‑141 (MQ=255)

                                                        tGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGgcgc                                                                             >  1:45345/1‑141 (MQ=255)

                                                                      taCCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTa                                                               >  2:429038/1‑141 (MQ=255)

                                                                      taCCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTa                                                               >  2:374534/1‑141 (MQ=255)

                                                                        ccTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAAt                                                             <  2:633718/141‑1 (MQ=255)

                                                                        ccTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAAt                                                             <  2:32601/141‑1 (MQ=255)

                                                                         cTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATg                                                            >  2:51767/1‑141 (MQ=255)

                                                                         cTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATg                                                            >  2:51761/1‑141 (MQ=255)

                                                                         cTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATg                                                            >  1:376699/1‑141 (MQ=255)

                                                                                   tGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTTAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGa                                                  <  1:104801/141‑1 (MQ=255)

                                                                                   tGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGa                                                  <  1:429038/141‑1 (MQ=255)

                                                                                   tGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGa                                                  >  1:599861/1‑141 (MQ=255)

                                                                                    gCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGAt                                                 <  2:129412/141‑1 (MQ=255)

                                                                                      tGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGc                                               <  1:305228/141‑1 (MQ=255)

                                                                                           tgatgGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGa                                          >  1:642572/1‑141 (MQ=255)

                                                                                             atgGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGaaa                                        <  2:167929/141‑1 (MQ=255)

                                                                                             atgGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGaaa                                        <  1:555879/141‑1 (MQ=255)

                                                                                                gATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAagag                                     <  2:229880/141‑1 (MQ=255)

                                                                                                gATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAagag                                     <  1:301061/141‑1 (MQ=255)

                                                                                                 atatCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGc                                    >  2:636048/1‑141 (MQ=255)

                                                                                                 atatCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGc                                    <  1:368292/141‑1 (MQ=255)

                                                                                                    tCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAAt                                                                      >  2:329933/1‑104 (MQ=255)

                                                                                                    tCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAAt                                                                      <  1:329933/104‑1 (MQ=255)

                                                                                                      tATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAAtt                               <  2:176130/141‑1 (MQ=255)

                                                                                                       aTGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAAttt                              <  2:325240/141‑1 (MQ=255)

                                                                                                         gCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTta                            <  2:262167/141‑1 (MQ=255)

                                                                                                          cGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTtat                           <  1:620306/141‑1 (MQ=255)

                                                                                                          cGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTtat                           <  1:425074/141‑1 (MQ=255)

                                                                                                              tGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTTATACGa                       <  2:428661/141‑1 (MQ=255)

                                                                                                                  cTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTTATACGAAAtt                   <  2:222255/141‑1 (MQ=255)

                                                                                                                    gAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTTATACGAAATTaa                 <  2:45345/141‑1 (MQ=255)

                                                                                                                             aCTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTTATACGAAATTAATCCAGAAGc        >  1:182527/1‑141 (MQ=255)

                                                                                                                                aaTTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTTATACGAAATTAATCCAGAAGCGGa     >  1:487626/1‑141 (MQ=255)

                                                                                                                                   tGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACAt                                                                         >  2:394706/1‑70 (MQ=255)

                                                                                                                                   tGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACAt                                                                         <  1:394706/70‑1 (MQ=255)

                                                                                                                                   tGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTTATACGAAATTAATCCAGAAGCGGaaaa  >  1:2281/1‑141 (MQ=255)

ACTAAGATGATTATGCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATGGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTTATACGAAATTAATCCAGAAGCGGAAAA  >  CP000730/603824‑604095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A