Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F67 I1 R2 13 27.5 561530 92.1% 517169 135.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 603,961 G→A M465I (ATG→ATA)  araB → ribulokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730603,9610GA100.0% 83.0 / NA 25M465I (ATG→ATA) araBribulokinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/10);  total (15/10)

TAAGATGATTATGCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATGGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTTATACGAAATTAATCCAGAAGCGGA  >  CP000730/603826‑604092
                                                                                                                                       |                                                                                                                                   
tAAGATGATTATGCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTatagatag                                                                                                                                <  1:15842/141‑1 (MQ=255)
     tgatTATGCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGt                                                                                                                           >  2:152051/1‑141 (MQ=255)
          aTGCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCa                                                                                                                      >  2:184303/1‑141 (MQ=255)
                    aTCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAg                                                                                                            >  1:17910/1‑141 (MQ=255)
                     tCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAgg                                                                                                           >  1:270550/1‑141 (MQ=255)
                         gATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCgca                                                                                                       <  1:184303/141‑1 (MQ=255)
                         gATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCgca                                                                                                       <  1:272075/141‑1 (MQ=255)
                           ttGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCgcagc                                                                                                     <  2:78091/141‑1 (MQ=255)
                                atatGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAatat                                                                                                >  1:142758/1‑141 (MQ=255)
                                          aGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGcagt                                                                                      >  2:125083/1‑141 (MQ=255)
                                                     ttGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGgcg                                                                           <  2:17910/141‑1 (MQ=255)
                                                      tGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGgcgc                                                                          >  1:36926/1‑141 (MQ=255)
                                                                    taCCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTa                                                            >  1:10389/1‑141 (MQ=255)
                                                                                 tGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGa                                               <  2:270550/141‑1 (MQ=255)
                                                                                  gCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGAt                                              >  1:58909/1‑141 (MQ=255)
                                                                                   cTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATg                                             <  1:240247/141‑1 (MQ=255)
                                                                                              gATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAagag                                  <  1:152051/141‑1 (MQ=255)
                                                                                               atatCGATGTGAATGTACTGTATAACAAACTAATTGTTATAGATAGTGAGAATGCACAAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGCTGCTATGaaggtgc                                 <  2:185229/141‑6 (MQ=255)
                                                                                                tatCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGcc                                <  2:142758/141‑1 (MQ=255)
                                                                                                   cTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAAt                             >  2:3780/1‑141 (MQ=255)
                                                                                                     aTGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAAttt                           >  1:141349/1‑141 (MQ=255)
                                                                                                             gTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGGGGTGGCGCATATAATTCAATTAATCACGCAGTTGATGCTATGAAAGAGCCAATTTTAAACGaa                   >  2:6421/1‑141 (MQ=255)
                                                                                                             gTACTGAATAAAAAACTAATTGTAATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTTATACGaa                   >  2:6428/1‑141 (MQ=255)
                                                                                                              tACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTTATACGaaa                  <  2:277017/141‑1 (MQ=255)
                                                                                                                          aaCTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTAAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTTATACGAAATTAATCCAGAAg      >  1:60578/1‑141 (MQ=255)
                                                                                                                              aaTTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGACGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTTATACGAAATTAATCCAGAAGCGGa  >  2:18732/1‑141 (MQ=255)
                                                                                                                                       |                                                                                                                                   
TAAGATGATTATGCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATGGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTTATACGAAATTAATCCAGAAGCGGA  >  CP000730/603826‑604092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A