Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A15 F177 I1 R2	35	38.3	831348	95.5%	793937	139.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,709,600	(T)_5→4	coding (1327/1812 nt)	oatA ←	possible acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,709,596	0	T	.	100.0%	121.6 / NA	26	coding (1331/1812 nt)	oatA	possible acetyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (14/12); total (14/12)

TATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAGTGCAT  >  CP000730/2709456‑2709728

tataTTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACt                                                                                                                                      <  2:331207/141‑1 (MQ=255)

     tGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACttttaa                                                                                                                                 >  1:338984/1‑139 (MQ=255)

     tGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACttttaa                                                                                                                                 <  1:328741/141‑3 (MQ=255)

      gCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACttttaat                                                                                                                                >  1:276296/1‑138 (MQ=255)

       cGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACttttaata                                                                                                                               <  1:287700/141‑5 (MQ=255)

                 aTTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGc                                                                                                                    >  1:217578/1‑141 (MQ=255)

                   tGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTa                                                                                                                  >  1:144142/1‑141 (MQ=255)

                      tgtAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTc                                                                                                               <  2:203136/141‑1 (MQ=255)

                                   aGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTGTAATATCCTCTTTGCTATTCGCTGTCTGCTTAt                                                                                                  <  2:266089/141‑1 (MQ=255)

                                    gTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATc                                                                                                 >  2:41719/1‑141 (MQ=255)

                                          gTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTg                                                                                           <  1:275876/141‑1 (MQ=255)

                                           tCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGc                                                                                          <  2:277019/141‑1 (MQ=255)

                                              aaCTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTa                                                                                       >  1:158799/1‑141 (MQ=255)

                                                       aTCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTa                                                                              >  1:126349/1‑141 (MQ=255)

                                                        tCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTaa                                                                             >  1:273906/1‑141 (MQ=255)

                                                          aaTTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACg                                                                           <  1:407614/141‑1 (MQ=255)

                                                             ttGTGCATTTGGTATTCTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTACCGACt                                                                        <  2:7337/141‑1 (MQ=255)

                                                                   aTTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTttc                                                                  <  2:223633/141‑1 (MQ=255)

                                                                        gTATTTTCTTAGTAAAGACATGACCAATATCCACCATGACCGATTCACCATTTAAGAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGTCTTTTttcttctt                                                             >  2:246625/1‑139 (MQ=255)

                                                                            tttCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCgttgtt                                                         >  1:361854/1‑141 (MQ=255)

                                                                             ttCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGttt                                                        >  1:373682/1‑141 (MQ=255)

                                                                                 tAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTaaa                                                    >  1:287765/1‑141 (MQ=255)

                                                                                             tACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGtcttctt                                        <  2:239267/141‑1 (MQ=255)

                                                                                             tACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGtcttctt                                        >  2:21019/1‑141 (MQ=255)

                                                                                             tACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTCTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGtcttctt                                        >  2:390011/1‑141 (MQ=255)

                                                                                                   tatCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCtcttt                                  <  1:215096/141‑1 (MQ=255)

                                                                                                   tatCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCtcttt                                  <  1:408834/141‑1 (MQ=255)

                                                                                                                  aGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCCTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCAtg                   <  2:7096/141‑1 (MQ=255)

                                                                                                                               tAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAGt      >  1:275895/1‑141 (MQ=255)

                                                                                                                               tAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAGt      >  1:130838/1‑141 (MQ=255)

                                                                                                                                   ggtgATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAGTGCAt  <  2:151019/141‑1 (MQ=255)

TATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCCAAGTGCAT  >  CP000730/2709456‑2709728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A