Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A15 F59 I1 R1	25	48.3	927160	99.3%	920669	151.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,018,733	(A)_6→7	coding (186/810 nt)	mgt ←	transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,018,727	1	.	A	100.0%	129.4 / NA	38	L64F (TTA→TTT)	mgt	transglycosylase
Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (18/20); total (18/20)

CCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGAT‑AAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCA  >  CP000730/2018578‑2018869

ccTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGAT‑a                                                                                                                                               <  1:314376/151‑1 (MQ=255)

ccTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGAT‑a                                                                                                                                               <  1:163192/151‑1 (MQ=255)

   ttCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGAT‑aaaa                                                                                                                                            >  2:2515/1‑151 (MQ=255)

   ttCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGAT‑aaaa                                                                                                                                            <  2:429794/151‑1 (MQ=255)

         tCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGAT‑aaaaaaatac                                                                                                                                      <  2:264187/151‑5 (MQ=255)

          cGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGAT‑aaaaaaataca                                                                                                                                     <  2:414724/151‑6 (MQ=255)

                cATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATAc                                                                                                                                >  1:234126/1‑151 (MQ=255)

                  tgatgaTTGTAGAATCGTTCATATTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATAGAAAAAAAAAAAAAAAATACATGATACCa                                                                                                                              <  2:148296/151‑1 (MQ=255)

                   gatgatTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGAT‑aaaaaaataaatcttatcaa                                                                                                                            >  2:365195/1‑137 (MQ=255)

                          gTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACaa                                                                                                                      >  1:128871/1‑151 (MQ=255)

                          gTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACaa                                                                                                                      >  1:246110/1‑151 (MQ=255)

                           tAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAAt                                                                                                                     <  1:2515/151‑1 (MQ=255)

                                 cGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATa                                                                                                               <  1:454670/151‑1 (MQ=255)

                                       tCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGAtt                                                                                                         <  2:263288/151‑1 (MQ=255)

                                        cTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTa                                                                                                        >  1:103408/1‑151 (MQ=255)

                                        cTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTa                                                                                                        >  1:409058/1‑151 (MQ=255)

                                        cTTCCATTGAAATAAAGGCACCTTTAACATACTCGGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTa                                                                                                        <  1:296873/151‑1 (MQ=255)

                                              ttGAAATAAAGTCACCTGTAGCATACCCTGACATGTTATCAGCTGAACCAAAACTACTTTTACTTTCAATTTTTCTTCGTTCATCCTCATTATGTCGTGTAGATAAAAAAATACATGGTCCCAATTATCAATGCGATAATGATTAGAATgg                                                                                                  <  2:305193/151‑1 (MQ=255)

                                              ttGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATgg                                                                                                  <  2:384186/151‑1 (MQ=255)

                                                gAAATAGAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGtt                                                                                                <  1:179415/151‑1 (MQ=255)

                                                 aaaTAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTta                                                                                               <  2:333823/151‑1 (MQ=255)

                                                 aaaTAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTta                                                                                               <  1:212439/151‑1 (MQ=255)

                                                   aTAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTtaat                                                                                             >  2:368368/1‑151 (MQ=255)

                                                   aTAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTtaat                                                                                             <  1:111976/151‑1 (MQ=255)

                                                                  aCATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGt                                                                              >  2:229303/1‑151 (MQ=255)

                                                                      aCTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTc                                                                          >  2:37782/1‑151 (MQ=255)

                                                                         ctGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGtt                                                                       >  1:454398/1‑151 (MQ=255)

                                                                            gCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAc                                                                    >  2:153821/1‑151 (MQ=255)

                                                                            gCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAc                                                                    >  2:258180/1‑151 (MQ=255)

                                                                            gCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAc                                                                    >  1:268621/1‑151 (MQ=255)

                                                                            gCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTAc                                                                    >  2:295143/1‑151 (MQ=255)

                                                                                          gACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAATATTCGTTTACTTtttttctttttt                                                      >  2:163098/1‑150 (MQ=255)

                                                                                                  actactTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGgtt                                              >  2:182513/1‑151 (MQ=255)

                                                                                                                       tCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGt                         <  1:260175/151‑1 (MQ=255)

                                                                                                                          tAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCGAATTGGTTGATAATACGtatt                      <  2:95299/151‑1 (MQ=255)

                                                                                                                          tAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGtatt                      <  2:53721/151‑1 (MQ=255)

                                                                                                                          tAGTACAGCCACATTATAGCGTGAAGATAAAAAAATACATGATACCAATAAGCAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGtatt                      <  2:14516/151‑1 (MQ=255)

                                                                                                                           aGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGtatta                     >  1:21802/1‑151 (MQ=255)

                                                                                                                           aGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGtatta                     >  1:233156/1‑151 (MQ=255)

                                                                                                                                          tCGCGTGTAGATAAAAAAATACATGTTACCAATAACCCATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCgtgt      <  1:210716/151‑1 (MQ=255)

                                                                                                                                          tCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCgtgt      <  1:313182/151‑1 (MQ=255)

                                                                                                                                          tCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCgtgt      <  1:202747/151‑1 (MQ=255)

                                                                                                                                           cgcgTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGtt     >  2:293341/1‑151 (MQ=255)

                                                                                                                                             cgTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTc   <  2:127035/151‑1 (MQ=255)

                                                                                                                                              gtgtAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCa  <  2:289188/151‑1 (MQ=255)

CCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGAT‑AAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGAGGTTCGTGTTTCA  >  CP000730/2018578‑2018869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A