Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A15 F177 I1 R2	35	38.3	831348	95.5%	793937	139.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,018,733	(A)_6→7	coding (186/810 nt)	mgt ←	transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,018,727	1	.	A	100.0%	128.3 / NA	37	L64F (TTA→TTT)	mgt	transglycosylase
Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (14/23); total (14/23)

CGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGAT‑AAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGA  >  CP000730/2018588‑2018856

cGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCTTTTTTTTTTTGTTCATCCACATTATCTCGTGTAGAT‑a                                                                                                                                  >  2:271203/1‑141 (MQ=255)

  aaTCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGAT‑aaa                                                                                                                                <  1:136910/141‑1 (MQ=255)

                gTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATATTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATAc                                                                                                                   >  1:298098/1‑141 (MQ=255)

                 tAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATAcc                                                                                                                  >  2:386552/1‑141 (MQ=255)

                    caTCGTTCATCTTCCACTGACATCAAGGCACCTTTAACATACTATGGCCTGTTATCAGCTGCCACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAAt                                                                                                               <  2:86652/140‑1 (MQ=255)

                     aTCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATa                                                                                                              <  1:65953/141‑1 (MQ=255)

                          aaaaaatacATTGAAATAAAGGCACCTTTAACATAATCTGGCATGTTATCAGCCGACACAAAACTACTTTTAATTTAAAATTTTCTTAGTTCATCCACATTATAGAAAAAAAAAAAAAAAATACATGATACCAATAAACaa                                                                                                         <  2:301738/133‑1 (MQ=255)

                               ttCCATTGAAATAAAGGCACCTTTAACATCCTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGa                                                                                                    <  1:413861/141‑1 (MQ=255)

                               ttCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGa                                                                                                    <  1:149902/141‑1 (MQ=255)

                                      aaaaTAAAGGAAGCTTTAAGATACTTTGGCATGATATCAGCAGACACAAAACTACTTTTATTTTAAATTTTTCTTAGTTCATCCACATTATAAAAAAAAAAAAAAAAAATACATGATACCAATAAACAATGCGATAATGAt                                                                                             <  2:300622/140‑1 (MQ=255)

                                         aTAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAg                                                                                          >  2:227015/1‑141 (MQ=255)

                                         aTAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAg                                                                                          <  1:54279/141‑1 (MQ=255)

                                            aaGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAAt                                                                                       >  1:110315/1‑141 (MQ=255)

                                                  ccTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGCTTAGAATGGTtaa                                                                                 >  1:353236/1‑141 (MQ=255)

                                                            aCTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTTTTTTTTTTTCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATAttttt                                                                       >  2:98914/1‑141 (MQ=255)

                                                             ctctGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTa                                                                      <  1:180664/141‑1 (MQ=255)

                                                                  gCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTTTTTTTTTTTCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGt                                                                 >  2:262831/1‑141 (MQ=255)

                                                                      gTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTc                                                             <  1:302610/141‑1 (MQ=255)

                                                                       ttATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTct                                                            >  2:9868/1‑140 (MQ=255)

                                                                       ttATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCg                                                            >  1:169147/1‑141 (MQ=255)

                                                                         aTCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTtaataa                                                                              >  2:113698/1‑121 (MQ=255)

                                                                         aTCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTtaataa                                                                              <  1:113698/121‑1 (MQ=255)

                                                                          tCAGCTGACACAAGACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGttt                                                         <  2:5340/141‑1 (MQ=255)

                                                                                 acacAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACttttt                                                  >  2:330803/1‑141 (MQ=255)

                                                                                      aaaCTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTtttttctt                                             >  1:207635/1‑141 (MQ=255)

                                                                                        actactTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTtttttctttt                                           <  1:138568/141‑1 (MQ=255)

                                                                                         ttactTTTATTTTCAATTTTTCTTATTTCATCCACATTATCGCTTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTTATAGTATTCGTTTACTTtttttcttgtt                                          <  2:350358/140‑1 (MQ=255)

                                                                                                       aaTTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTTGGCGGTTTGCCaa                            >  2:116333/1‑141 (MQ=255)

                                                                                                        aTTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAAc                           <  1:330803/141‑1 (MQ=255)

                                                                                                            ttCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGt                       <  1:269805/141‑1 (MQ=255)

                                                                                                            ttCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGt                       <  1:73415/141‑1 (MQ=255)

                                                                                                                tAGTTCATCCACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGat                   <  1:11610/141‑1 (MQ=255)

                                                                                                                        ccACATTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGta           <  1:85992/141‑1 (MQ=255)

                                                                                                                         cacaTTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGtat          <  2:110315/141‑1 (MQ=255)

                                                                                                                           caTTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGtatta        <  1:156442/141‑1 (MQ=255)

                                                                                                                           caTTATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGtatta        >  1:239251/1‑141 (MQ=255)

                                                                                                                             ttATCGCGTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATtata      <  1:236896/141‑1 (MQ=255)

                                                                                                                               aTCGCGTGTAAATAAAAAAATATATGATACCAATAAATGTTGGGATAATGTTTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTATTTTTTTTCTTTTTTCGCGGTTAGGCAACTATTTTATGATATGTATTTTAGt    <  2:186306/141‑1 (MQ=255)

                                                                                                                                 cgcgTGTAGATAAAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGa  <  2:146361/141‑1 (MQ=255)

CGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGAT‑AAAAAATACATGATACCAATAAACAATGCGATAATGATTAGAATGGTTAATAATATTTTTAATAGTATTCGTTTACTTTTTTTCTTTTTCGGCGGTTTGCCAACTGGTTGATAATACGTATTATAGTGA  >  CP000730/2018588‑2018856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A