Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F109 I1 R1
|
27 |
23.1 |
511165 |
92.8% |
474361 |
140.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
CP000730 |
134,393 |
G→A |
L12L (TTG→TTA) |
sbnA → |
possible pyridoxal‑phosphate dependent enzyme |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP000730 | 134,393 | 0 | G | A | 100.0%
| 56.6
/ NA
| 20 | L12L (TTG→TTA) | sbnA | possible pyridoxal‑phosphate dependent enzyme |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (12/8); total (12/8) |
TTACTTGATAGTGAGAATCATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTGTTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATGAATCCTGGAGGCAGCATGAAAGATCGACCTGCCAAG > CP000730/134281‑134513
|
ttACTTGATAGTGAGAATCATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATgg > 2:182774/1‑141 (MQ=255)
ttGATAGTGAGAATCATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCa > 2:256246/1‑141 (MQ=255)
aaaacATTACCAATTAAGTAACACACAAAAAGATAGAATTTGAAATCTGAGGAGGAAGCTAAAAAAAAAGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCaa < 2:481444/137‑1 (MQ=255)
cATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCGACTAt > 2:361339/1‑141 (MQ=255)
tcagatAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGCAGCCCTTTTGATTGAATAAAGTCAAGCATTGCACGATTAATTATTAGATCCTGTAGGGCAAACACCTATGGGTCAACTTCATCAACTATTTCCg < 2:73022/136‑1 (MQ=255)
tAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATCTCCGAAACa > 2:467576/1‑141 (MQ=255)
tAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCTCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTATATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGaa < 2:469787/141‑1 (MQ=255)
acacaATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAgtgt < 2:119082/141‑1 (MQ=255)
aatttaataGAAATTTAAATCTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGc < 2:215943/135‑1 (MQ=255)
attatAGAATTTGAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTGCATCAACTATTTCCGAAACATGAAGTGTTTGCaaa < 2:374029/141‑1 (MQ=255)
aaaTTTGAGGAGGCAGCGCTATTGATTGAAAAAAGTCAAGCACGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATg > 2:470915/1‑141 (MQ=255)
aaaTTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATg > 2:150128/1‑141 (MQ=255)
aaaTTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATg > 2:156400/1‑141 (MQ=255)
aTTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCGACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAGGTTAGAGAATATGaa > 2:199015/1‑141 (MQ=255)
aTTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATGaa > 2:401944/1‑141 (MQ=255)
gaggaAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGAATATGAATCCTGGa > 2:409050/1‑141 (MQ=255)
attgaAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATGAATCCTGGAGGCAGCATGAAAGAt > 2:495728/1‑141 (MQ=255)
attgaAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATGAATCCTGGAGGCAGCATGAAAGAt < 2:6049/141‑1 (MQ=255)
aaaaaGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATGAATCCTGGAGGCAGCATGAAAGATCGAcc < 2:486301/141‑1 (MQ=255)
cAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATGAATCCTGGAGGCAGCATGAAAGATCGACCTGCCAAg > 2:191848/1‑141 (MQ=255)
|
TTACTTGATAGTGAGAATCATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTGTTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATGAATCCTGGAGGCAGCATGAAAGATCGACCTGCCAAG > CP000730/134281‑134513
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
