Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F109 I1 R1
|
27 |
23.1 |
511165 |
92.8% |
474361 |
140.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
CP000730 |
2,352,815 |
G→A |
A92V (GCA→GTA) |
rpsI ← |
ribosomal protein S9 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP000730 | 2,352,815 | 0 | G | A | 94.4%
| 33.9
/ ‑3.2
| 19 | A92V (GCA→GTA) | rpsI | ribosomal protein S9 |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (10/7); total (11/8) |
| Fisher's exact test for biased strand distribution p-value = 4.44e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.90e-01 |
CCGACAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTAAGTC > CP000730/2352684‑2352945
|
ccGACAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCtaata < 1:2723/141‑1 (MQ=255)
aCAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTGCTAATACTg > 2:345305/1‑141 (MQ=255)
gagttAGCGTTTTGAGAATGGGGGGGAACGACGAGCTGCGTTAAGAGCTGGTTTTTTGCGTTTGTTCAGACGTGGGTGACGAGGAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGc < 2:425559/138‑1 (MQ=255)
cAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTCCTTTCATACGTGGGTCACGAGTAAGTACTCCAGCGCGGTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAGTAGTGc > 2:71176/1‑141 (MQ=38)
tttGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTCCGTTCTTTCCTACCTGGGTCCCCACTAAGTCATCCAGCGCGTTTTCCAGAACCTCTGTATTCAGGCTCTACTTCTAATCATGCACGAGCGAtt > 2:177/1‑141 (MQ=37)
aGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTTTTTTTTTTTTTACGTAGGACACGCGTAAGTAATCCAACGCTGGTTAAAGAATCTCTATCTTCTGGATCTCCTCCCAATAATGCACGAGCGATTCCGTGACggct > 2:235766/1‑139 (MQ=255)
aGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAAGATCTACTTCTAATAATGCACGAGCGATTCCATGagcgat > 2:476664/1‑136 (MQ=255)
tGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAaccacc < 2:408338/141‑1 (MQ=255)
gatctttACGTGCCTGCATACGCGGGTCACGAGCAAGTAATCCAGCGCGTTTTAAAGAACAGCTGGATTCAGGATCTACTGCTAATAAGGCACGAGCCAGCCCGTGACGGATAGCTTGAGCTTGACCAGCGGAacaacccc > 2:231912/5‑139 (MQ=255)
ttttttACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAaccaccccc > 2:461833/1‑141 (MQ=255)
tttttACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAAccaccacca > 2:417347/1‑141 (MQ=255)
gTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAAcat < 2:213841/141‑1 (MQ=255)
gtaagtaaTCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTCCCt > 2:37299/1‑141 (MQ=255)
taagtaaTCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCtt < 2:423654/141‑1 (MQ=255)
cgcgTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGCAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTa > 2:469581/1‑141 (MQ=255)
gTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCGGTTACAt > 2:153260/1‑141 (MQ=255)
tgtAAAGATCCTGTGTATGCAGGATCTACTTTGTAGTATGCACGAGCGATTCCGTGACGGATAGTTTGAGCTTGTCCCGTGAGACCACCACCATGAACGTTAGCTAAAACATCATAGTTAGCTTTAGTTTCAGTTACATCa < 2:331967/139‑1 (MQ=255)
tcactacccTCTGTATTCAGGATCTACTTGTAATAATGCACGATCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTATCTTTAGTTTCAGTTACATCaaa < 2:275104/134‑1 (MQ=255)
ttCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTAAGTc < 2:455090/141‑1 (MQ=255)
|
CCGACAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTAAGTC > CP000730/2352684‑2352945
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
