Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A19 F34 I0 R1 470 116.5 4231616 98.1% 4151215 142.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 894,952 T→A 5.5% intergenic (+56/‑39) potF → / → potG putrescine ABC transporter periplasmic binding protein/putrescine ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913894,9520TA 5.5% 312.3 / 7.3 91intergenic (+56/‑39)potF/potGputrescine ABC transporter periplasmic binding protein/putrescine ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base T (45/41);  new base A (3/2);  total (48/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01

TTCGTGCGAAGCTGTTCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGAT  >  NC_000913/894805‑895098
                                                                                                                                                   |                                                                                                                                                   
ttCGTGCGAAGCTGTTCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTa                                                                                                                                                    >  2:339554/1‑149 (MQ=255)
  cGTGCGAAGCTGTTCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATg                                                                                                                                                  <  4:826290/149‑1 (MQ=255)
       gAAGCTGTTCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTGGCACCATTATGGTgcg                                                                                                                                             >  4:865424/1‑149 (MQ=255)
       gAAGCTGTTCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTgcg                                                                                                                                             >  3:663222/1‑149 (MQ=255)
        aaGCTGTTCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTgcg‑c                                                                                                                                           >  1:1036067/1‑149 (MQ=255)
         aGCTGTTCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGGGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATAATGGTgcgaa                                                                                                                                           <  3:1027906/149‑3 (MQ=255)
          gCTGTTCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑Ctt                                                                                                                                         >  1:757076/1‑149 (MQ=255)
            tGTTCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGc                                                                                                                                       <  1:471587/149‑1 (MQ=255)
                 aCTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGcacaca                                                                                                                                  >  3:445242/1‑149 (MQ=255)
                   tctGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACTCCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACAtt                                                                                                                                >  1:871944/1‑149 (MQ=255)
                      gAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCaa                                                                                                                             <  1:794313/149‑1 (MQ=255)
                         aGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGc                                                                                                                          >  4:56265/1‑149 (MQ=255)
                           tGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAACATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCg                                                                                                                        >  1:808153/1‑149 (MQ=255)
                           tGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGc                                                                                                                          >  2:221715/1‑147 (MQ=255)
                           tGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGc                                                                                                                          <  1:221715/147‑1 (MQ=255)
                           tGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCg                                                                                                                        <  4:453140/149‑1 (MQ=255)
                           tGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCg                                                                                                                        <  2:404761/149‑1 (MQ=255)
                             cAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGga                                                                                                                      <  2:822799/149‑1 (MQ=255)
                                    cGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGagcagc                                                                                                               <  4:251997/149‑1 (MQ=255)
                                    cGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGAGAAGAGAGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGagcagc                                                                                                               <  4:445242/149‑1 (MQ=255)
                                     gAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGcaca                                                                                                                                    >  4:1030301/1‑127 (MQ=255)
                                     gAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGcaca                                                                                                                                    <  3:1030301/127‑1 (MQ=255)
                                     gAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGagcag                                                                                                                <  4:602732/147‑1 (MQ=255)
                                     gAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGagcag                                                                                                                >  3:602732/1‑147 (MQ=255)
                                     gAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGcc                                                                                                              >  3:985290/1‑149 (MQ=255)
                                     gAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGcc                                                                                                              >  1:417602/1‑149 (MQ=255)
                                     gAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGcc                                                                                                              >  4:104652/1‑149 (MQ=255)
                                     gAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGcc                                                                                                              >  4:698780/1‑149 (MQ=255)
                                     gAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGcc                                                                                                              <  1:154315/149‑1 (MQ=255)
                                           cGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAAt                                                                                                        <  1:604648/149‑1 (MQ=255)
                                           cGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAAt                                                                                                        <  2:96037/149‑1 (MQ=255)
                                           cGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAAt                                                                                                        >  2:22404/1‑149 (MQ=255)
                                             aCCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATAATGGTgcgaa                                                                                                                                           >  3:89507/1‑111 (MQ=255)
                                             aCCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATAATGGTgcgaa                                                                                                                                           <  4:89507/113‑3 (MQ=255)
                                               cGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACg                                                                                                    <  1:1016082/149‑1 (MQ=255)
                                                 tgtgCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATg                                                                                                                                                  <  1:166081/102‑1 (MQ=255)
                                                 tgtgCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATg                                                                                                                                                  >  2:166081/1‑102 (MQ=255)
                                                         cccGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCg                                                                                          >  2:794532/1‑149 (MQ=255)
                                                                gTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAg                                                                                   >  2:634990/1‑149 (MQ=255)
                                                                     ccAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGaa                                                                              >  1:1002909/1‑149 (MQ=255)
                                                                      cAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGaaa                                                                             >  1:624466/1‑149 (MQ=255)
                                                                        aaGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAAc                                                                           >  1:161687/1‑149 (MQ=255)
                                                                        aaGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGCGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAAc                                                                           >  3:643840/1‑149 (MQ=255)
                                                                          gTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACTTTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAAccc                                                                         <  2:871944/149‑1 (MQ=255)
                                                                            gAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGaa                                                                                                         <  3:730867/115‑1 (MQ=255)
                                                                            gAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGaa                                                                                                         >  4:730867/1‑115 (MQ=255)
                                                                             aaGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTa                                                                      >  1:52759/1‑149 (MQ=255)
                                                                              agagCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTaa                                                                     >  2:10915/1‑149 (MQ=255)
                                                                               gagCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAg                                                                    <  1:634990/149‑1 (MQ=255)
                                                                                agCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAgg                                                                   >  1:931524/1‑149 (MQ=255)
                                                                                   ggAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCCCGCCTGCAGGCGAAAACCCGTAAGgcgc                                                                <  4:645573/149‑1 (MQ=255)
                                                                                    gAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCt                                                               >  1:679312/1‑149 (MQ=255)
                                                                                     aaaaTAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTg                                                              >  2:908294/1‑149 (MQ=255)
                                                                                          aatCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGAcgcc                                                         <  4:606492/149‑1 (MQ=255)
                                                                                          aatCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGAcgcc                                                         <  1:218466/149‑1 (MQ=255)
                                                                                            tCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATc                                                                                               <  4:26877/109‑1 (MQ=255)
                                                                                            tCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATc                                                                                               >  3:26877/1‑109 (MQ=255)
                                                                                                 aGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTa                                                  <  3:616658/149‑1 (MQ=255)
                                                                                                 aGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTa                                                  <  2:679312/149‑1 (MQ=255)
                                                                                                  gTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAg                                                 <  1:195309/149‑1 (MQ=255)
                                                                                                     gTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGaaa                                              >  3:370395/1‑149 (MQ=255)
                                                                                                        gATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATcc                                           <  1:274424/149‑1 (MQ=255)
                                                                                                            ccGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCaa                                       <  4:151122/149‑1 (MQ=255)
                                                                                                             cGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAAc                                      <  1:339554/149‑1 (MQ=255)
                                                                                                              ggAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAAcc                                     >  4:732809/1‑149 (MQ=255)
                                                                                                                 ggggCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGa                                  <  3:732809/149‑1 (MQ=255)
                                                                                                                  gggCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGAc                                 <  1:924751/149‑1 (MQ=255)
                                                                                                                        accacACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATc                           >  2:512047/1‑149 (MQ=255)
                                                                                                                             acCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGAcgc                                                          >  4:422384/1‑113 (MQ=255)
                                                                                                                             acCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGAcgc                                                          <  3:422384/113‑1 (MQ=255)
                                                                                                                                  ccgGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGt                 <  1:272619/149‑1 (MQ=255)
                                                                                                                                    gtgcaagcGCACCATAATGGTGCGAATTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTc                >  1:369710/8‑149 (MQ=255)
                                                                                                                                      cAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAAc             >  3:957762/1‑149 (MQ=255)
                                                                                                                                       aaTTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATAACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACa            >  1:576640/1‑149 (MQ=255)
                                                                                                                                        aTTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACAt           >  4:1005172/1‑149 (MQ=255)
                                                                                                                                         ttCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATg          >  1:548518/1‑149 (MQ=255)
                                                                                                                                          tCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGc         <  1:345531/149‑1 (MQ=255)
                                                                                                                                            gCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTa                                                  >  1:282366/1‑106 (MQ=255)
                                                                                                                                            gCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTa                                                  <  2:282366/106‑1 (MQ=255)
                                                                                                                                            gCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCgg       <  2:271163/149‑1 (MQ=255)
                                                                                                                                            gCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCgg       >  1:875314/1‑149 (MQ=255)
                                                                                                                                            gCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCgg       <  1:888709/149‑1 (MQ=255)
                                                                                                                                            gCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCgg       >  2:254015/1‑149 (MQ=255)
                                                                                                                                             cACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGt      >  4:463951/1‑149 (MQ=255)
                                                                                                                                             cACCATTATGGTGCG‑CTTGCACACATTCAATGCCGAAGAGCAGCCGTGAACGCCGCTCCCCCTCGCCCCCAGGCGAAAACCCGTAAGGCACTGACGACGCTAATAAAAATACGCAACCCGACCACAGACTACGATGGAAAACATCCGGt      <  2:825135/149‑1 (MQ=255)
                                                                                                                                              aCCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTg     <  2:1036067/149‑1 (MQ=255)
                                                                                                                                                cATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGga   >  1:982278/1‑149 (MQ=255)
                                                                                                                                                cATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGga   <  2:417602/149‑1 (MQ=255)
                                                                                                                                                cATAATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGga   >  2:925467/1‑149 (MQ=255)
                                                                                                                                                 aTTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGgat  <  4:259513/149‑1 (MQ=255)
                                                                                                                                                 aTTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGgat  >  2:361908/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TTCGTGCGAAGCTGTTCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCG‑CTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGAT  >  NC_000913/894805‑895098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A