Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A13 F69 I0 R1 1426 148.0 5875178 97.4% 5722423 123.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,318,470 G→A 9.1% A440V (GCA→GTA) ‡ trpC ← indole‑3‑glycerolphosphate synthetase and N‑(5‑phosphoribosyl)anthranilate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,318,4700GA 9.1% 197.9 / 10.2 66A440V (GCA→GTA) ‡trpCindole‑3‑glycerolphosphate synthetase and N‑(5‑phosphoribosyl)anthranilate isomerase
Reads supporting (aligned to +/- strand):  ref base G (30/30);  new base A (3/3);  total (33/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.98e-01

TTCCAGCTGGCGCAGAGCAGGCATCAGGATTTGTGGCACGTACATGCCGCCAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAACGTTGCCAAGCGATTGACCATTTAATAGTGACCAG  >  NC_000913/1318329‑1318616
                                                                                                                                             |                                                                                                                                                  
ttcCAGCTGGCGCAGAGCAGGCATCAGGATTTGTGGCACGTACATGCCGCCAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCttt                                                                                                                                             >  2:504738/1‑149 (MQ=255)
  ccAGCTGGCGCAGAGCAGGCATCAGGATTTGTGGCACGTACATGCCGCCAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGa                                                                                                                                           <  1:504738/149‑1 (MQ=255)
     gCTGGCGCAGAGCAGGCATCAGGATTTGTGGCACGTACATGCCGCCAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGATGc                                                                                                                                        >  3:715845/1‑149 (MQ=255)
         gcgcAGAGCAGGCATCAGGATTTGTGGCACGTACATGCCGCCAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCgg                                                                                                                                    >  3:989444/1‑149 (MQ=255)
           gcAGAGCAGGCATCAGGATTTGTGGCACGTACATGCCGCCAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGa                                                                                                                                           >  3:190451/1‑140 (MQ=255)
           gcAGAGCAGGCATCAGGATTTGTGGCACGTACATGCCGCCAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGa                                                                                                                                           <  4:190451/140‑1 (MQ=255)
           gcAGAGCAGGCATCAGGATTTGTGGCACGTACATGCCGCCAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGCACGTCtttt                                                                                                                                            >  1:42482/1‑138 (MQ=255)
           gcAGAGCAGGCATCAGGATTTGTGGCACGTACATGCCGCCAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGCACGTCtttt                                                                                                                                            <  2:42482/139‑2 (MQ=255)
                    gCATCAGGATTTGTGGCACGTACATGCCGCCAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGc                                                                                                                                                   >  4:155079/1‑123 (MQ=255)
                    gCATCAGGATTTGTGGCACGTACATGCCGCCAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGc                                                                                                                                                   <  3:155079/123‑1 (MQ=255)
                      aTCAGGATTTGTGGCACGTACATGCCGCCAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGa                                                                                                                                           >  2:1243653/1‑129 (MQ=255)
                      aTCAGGATTTGTGGCACGTACATGCCGCCAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGa                                                                                                                                           <  1:1243653/129‑1 (MQ=255)
                       tCAGGATTTGTGGCACGTACATGCCGCCAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGATGccc                                                                                                                                      >  2:1414123/1‑133 (MQ=255)
                       tCAGGATTTGTGGCACGTACATGCCGCCAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGATGccc                                                                                                                                      <  1:1414123/133‑1 (MQ=255)
                       tCAGGATTTGTGGCACGTACATGCCGCCAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGtt                                                                                                                                  <  4:423603/137‑1 (MQ=255)
                       tCAGGATTTGTGGCACGTACATGCCGCCAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGtt                                                                                                                                  >  3:423603/1‑137 (MQ=255)
                       tCAGGATTTGTGGCACGTACATGCCGCCAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTAcag                                                                                                                      >  3:649503/1‑149 (MQ=255)
                       tCAGGATTTGTGGCACGTACATGCCGCCAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTAcag                                                                                                                      >  1:451750/1‑149 (MQ=255)
                         aGGATTTGTGGCACGTACATGCCGCCAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTAcagca                                                                                                                    <  2:451750/149‑1 (MQ=255)
                          ggATTTGTGGCACGTACATGCCGCCAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTAcagcag                                                                                                                   <  1:97291/149‑1 (MQ=255)
                           gATTTGTGGCACGTACATGCCGCCAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGc                                                                                                                                                   >  2:708925/1‑116 (MQ=255)
                           gATTTGTGGCACGTACATGCCGCCAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGc                                                                                                                                                   <  1:708925/116‑1 (MQ=255)
                            aTTTGTGGCACGTACATGCCGCCAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGaa                                                                                                                 <  2:1362919/149‑1 (MQ=255)
                                gtgGCACGTACATGCCGCCAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTaa                                                                                                             <  1:288799/149‑1 (MQ=255)
                                     aCGTACATGCCGCCAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTaaaa                                                                                                           >  4:23665/1‑146 (MQ=255)
                                     aCGTACATGCCGCCAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTaaaa                                                                                                           <  3:23665/146‑1 (MQ=255)
                                      cGTACATGCCGCCAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCaa                                                                                                       <  4:649503/149‑1 (MQ=255)
                                       gTACATGCCGCCAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAg                                                                                                      <  4:989444/149‑1 (MQ=255)
                                          cATGCCGCCAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTcc                                                                                                   <  4:715845/149‑1 (MQ=255)
                                           aTGCCGCCAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCg                                                                                                                                                  <  3:784013/101‑1 (MQ=255)
                                           aTGCCGCCAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCg                                                                                                                                                  >  4:784013/1‑101 (MQ=255)
                                           aTGCCGCCAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTa                                                                                                              <  4:270627/137‑1 (MQ=255)
                                           aTGCCGCCAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTa                                                                                                              >  3:270627/1‑137 (MQ=255)
                                                 ccAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTAca                                                                                                                       <  2:636452/122‑1 (MQ=255)
                                                 ccAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTAca                                                                                                                       >  1:636452/1‑122 (MQ=255)
                                                 ccAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAg                                                                                            >  2:1282675/1‑149 (MQ=255)
                                                       tCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGCACGTCtttt                                                                                                                                            <  3:820484/95‑2 (MQ=255)
                                                       tCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGCACGTCtttt                                                                                                                                            >  4:820484/1‑94 (MQ=255)
                                                                  ggggTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTc                                                                           >  4:1357376/1‑149 (MQ=255)
                                                                     gTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCAc                                                                        <  3:1357376/149‑1 (MQ=255)
                                                                         aGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAg                                                                    <  1:1282675/149‑1 (MQ=255)
                                                                           tAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCaa                                                                                                       >  3:113034/1‑112 (MQ=255)
                                                                           tAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCaa                                                                                                       <  4:113034/112‑1 (MQ=255)
                                                                           tAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGtt                                                                  >  1:1345871/1‑149 (MQ=255)
                                                                                ttgtCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTg                                                             >  1:481443/1‑149 (MQ=255)
                                                                                  gtCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTgcg                                                           >  2:391209/1‑149 (MQ=255)
                                                                                   tCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTgcgc                                                          <  2:481443/149‑1 (MQ=255)
                                                                                     aTTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGc                                                                                           <  2:822884/114‑1 (MQ=255)
                                                                                     aTTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGc                                                                                           >  1:822884/1‑114 (MQ=255)
                                                                                       tGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCt                                                                                                                                               >  3:1440379/1‑60 (MQ=255)
                                                                                       tGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCt                                                                                                                                               <  4:1440379/60‑1 (MQ=255)
                                                                                                  ttAATATGCGCGCAGCGTCTGGAAAACCGAGGTCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCa                                                                                                        <  1:1277627/88‑1 (MQ=255)
                                                                                                  ttAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCa                                                                                                        >  2:1277627/1‑88 (MQ=255)
                                                                                                           gcgcAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAACGt                                  <  1:391209/149‑1 (MQ=255)
                                                                                                                                  ccAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAACGtt                                 >  1:648794/1‑127 (MQ=255)
                                                                                                                                  ccAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAACGtt                                 <  2:648794/127‑1 (MQ=255)
                                                                                                                                  atcaaaGACGCACGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGtt                                                                                      >  3:336725/4‑74 (MQ=255)
                                                                                                                                  atcaaaGACGCACGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGtt                                                                                      <  4:336725/71‑1 (MQ=255)
                                                                                                                                   cAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGtt                                                                  <  4:208027/93‑1 (MQ=255)
                                                                                                                                   cAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGtt                                                                  >  3:208027/1‑93 (MQ=255)
                                                                                                                                    aaaaGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCaa                                                                                                       >  4:57460/1‑55 (MQ=255)
                                                                                                                                    aaaaGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCaa                                                                                                       <  3:57460/55‑1 (MQ=255)
                                                                                                                                    aaaaGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAACGTTGCCAAGCGATTGACCATTTAATa          >  1:523337/1‑148 (MQ=255)
                                                                                                                                    aaaaGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAACGTTGCCAAGCGATTGACCATTTAATa          <  2:523337/148‑1 (MQ=255)
                                                                                                                                    aaaaGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAACGTTGCCAAGCGATTGACCATTTAATAg         >  4:822946/1‑149 (MQ=255)
                                                                                                                                           gTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGGATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAACGTTGCCAAGCGATTGACCATTTAATAGTGACCAg  >  4:1067743/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                  
TTCCAGCTGGCGCAGAGCAGGCATCAGGATTTGTGGCACGTACATGCCGCCAAACTCACCAAAATAGGGGTTAAGTAATGTTGTCATTGTTCCTTTCCTTAATATGCGCGCAGCGTCTGGAAAACCGAGGCCAAAAGACGTGCGTCTTTGATGCCCGGTTGCGACTCTACAGCAGAATTAAAATCAAGTCCGGCGCAGCCGGTTTGTGCCGCTTCCACGCAGTTATCTGCGCCTAAGCCCCCCGCCAGCAGAACGTTGCCAAGCGATTGACCATTTAATAGTGACCAG  >  NC_000913/1318329‑1318616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A